Human Eyeblink Response Research Articles

Development of a Stimulation System for the Gustatory Eye-Blink Response in Humans

An inexpensive stimulation system for recording eye-blink responses elicited by taste stimuli has been developed using a manually controlled syringe and tasimetric sensor. The system requires neither an intra-oral device nor clamping of the head and tongue for natural eye-blink responses. The data recorded by the system have a high temporal resolution that is likely to be sufficient for the analysis of eye-blink responses based on video recordings.

The incremental stimulus intensity effect in the habituation of the eyeblink response in humans

Investigation of the eyeblink response of humans to auditory and tactile stimulation focused on the determinants of the so called incremental stimulus intensity effect. In Experiment 1, two groups of participants were exposed to 100 tones from 60 to 87-dB in intensity. In Group Incremental, the tones increased from 60- to 87-dB in 3-dB steps, whereas participants in Group Random received the same intensities in a pseudorandom order. The performance of the two groups was compared to Group Constant that received all 100 tones at 90-dB intensity and to a fourth group, named “Apparatus” that received no stimulation. In a subsequent testing block, the results indicated that there was less responding to a 90-dB tone in the Incremental than in the other three groups, which replicates the incremental stimulus intensity effect. The novel observation was that there was also significantly less responding in the Incremental than in the other three groups to an untrained tactile stimulus, suggesting that the differences between the conditions may have implicated differences in a more general sensitization, in addition to any differences in stimulus-specific habituation. Experiment 2 demonstrated that repetition of tones at a fixed intensity caused a decrease in responding to the tones, but also an increase in responding to a novel tactile stimulus, as compared to an untrained condition. These results are discussed in terms of the interpretation of the incremental stimulus intensity effect suggested by Davis and Wagner (1969) and the AESOP (Wagner & Brandon, 1989) treatment of habituation and sensitization.

Habituation of the eyeblink response in humans with stimuli presented in a sequence of incremental intensity.

In an experiment we examined whether the repeated presentation of tones of gradually increasing intensities produces greater decrement in the eyeblink reflex response in humans than the repetition of tones of constant intensities. Two groups of participants matched for their initial level of response were exposed to 110 tones of 100-ms duration. For the participants in the incremental group, the tones increased from 60- to 90- dB in 3-dB steps, whereas participants in the constant group received the tones at a fixed 90-dB intensity. The results indicated that the level of response in the last block of 10 trials, in which both groups received 90-dB tones, was significantly lower in the incremental group than in the constant group. These findings support the data presented by Davis and Wagner (7) with the acoustic response in rats, but differ from several reports with autonomic responses in humans, where the advantage of the incremental condition has not been observed unambiguously. The discussion analyzes theoretical approaches to this phenomenon and the possible involvement of separate neural circuits.

Habituation of the eyeblink response in humans with stimuli presented in a sequence of incremental intensity

In an experiment we examined whether the repeated presentation of tones of gradually increasing intensities produces greater decrement in the eyeblink reflex response in humans than the repetition of tones of constant intensities. Two groups of participants matched for their initial level of response were exposed to 110 tones of 100-ms duration. For the participants in the incremental group, the tones increased from 60- to 90- dB in 3-dB steps, whereas participants in the constant group received the tones at a fixed 90-dB intensity. The results indicated that the level of response in the last block of 10 trials, in which both groups received 90-dB tones, was significantly lower in the incremental group than in the constant group. These findings support the data presented by Davis and Wagner (7) with the acoustic response in rats, but differ from several reports with autonomic responses in humans, where the advantage of the incremental condition has not been observed unambiguously. The discussion analyzes theoretical approaches to this phenomenon and the possible involvement of separate neural circuits.

Eyeblink-related areas in human cerebellum as shown by fMRI.

Classical eyeblink conditioning is used frequently to study the role of the cerebellum in associative learning. To understand the mechanisms involved in learning, the neural circuits that generate the eyeblink response should be identified. The goal of the present study was to examine cerebellar regions that are likely to control the human eyeblink response using event-related functional magnetic resonance imaging (fMRI). In 14 healthy volunteers eyeblinks were evoked by unilateral air-puff stimulation (total of 30 stimuli, inter-trial interval 27-44 sec). With eyes closed throughout the experiment, eyeblinks were recorded using a video-based system with infrared reflecting markers being attached to the upper eyelids. From each subject 500 scans were taken (TR = 2.2 sec, 22 slices per scan, slice thickness = 3 mm) using an echo planar imaging sequence (EPI). The statistical parametric maps of the experimental volume images were estimated with SPM99 specifying an appropriate event-related design matrix. Two main regions of significant activation were found in the ipsilateral posterior lobe of the cerebellar hemisphere. In the more anterior region the maxima of activation were located in hemispheral lobules VI and Crus I, and in the more posterior region in hemispheral lobules VIIb, Crus II and VIIIa (nomenclature according to Schmahmann et al. [2000]: MRI Atlas of the Human Cerebellum). Although less pronounced, activity was found also in corresponding areas of the contralateral cerebellar hemisphere. These eyeblink-related areas agree with trigeminal projection areas and blink reflex control areas shown in previous animal studies.

The human eyeblink response: cerebellar and brainstem circuits as revealed by fMRI

The human eyeblink response: cerebellar and brainstem circuits as revealed by fMRI

The Effects of Scopolamine on Changes in Regional Cerebral Blood Flow during Classical Conditioning of the Human Eyeblink Response

We examined the effects of scopolamine on the functional anatomy of classical conditioning of the human eyeblink response. Ten healthy young normal female volunteers (mean age ± SEM: 26.7 ± 0.9 years) were administered 0.4 mg scopolamine intravenously 1 h before regional cerebral blood flow (rCBF) was measured with positron emission tomography (PET) and H₂¹⁵O. Scans occurred during three sequential phases: (1) explicitly unpaired presentations of the unconditioned stimulus (airpuff to the right eye) and conditioned stimulus (binaural tone), (2) paired presentations of the two stimuli (associative learning) and (3) explicitly unpaired presentation of the stimuli (extinction phase). Scopolamine impaired acquisition of the conditioned eyeblink response (54.7 ± 4.9%) relative to 18 untreated subjects from two previous PET studies. Regions that showed significant relative increases in rCBF during conditioning included the right lateral occipital cortex, the right inferior occipital cortex, the right lateral temporo-occipital cortex, the left medial temporo-occipital cortex, the posterior cingulate, the right cerebellum/brain stem area and the medial cerebellum. Significant relative decreases in rCBF were measured in the thalamus, the left putamen/insula area, the right putamen and the left and middle cerebellar cortex. The data partially replicate previous findings in unmedicated young volunteers of conditioning-specific rCBF changes in the cingulate cortex, the cerebellar cortex, the insula and the lateral temporo-occipital cortex. Our finding of decreased rCBF in the thalamus and increased rCBF in the occipital cortex may be attributable to effects of scopolamine per se rather than conditioning. Our data lend further support to the notion that classical conditioning involves distributed changes in multiple systems within the central nervous system.

The fear potentiated startle effect

A differential conditioning study examined whether an acoustic startle probe, presented during extinction of an aversively conditioned visual stimulus, potentiated the reflex eyeblink response in humans and whether this potentiation varied with the change in affective valence of the conditioned stimulus. Sixty college students were randomly assigned to view a series of two slides, depicting either unpleasant/highly arousing, unpleasant/moderate arousing, neutral/calm, pleasant/moderate arousing or pleasant/highly arousing scenes and objects (duration: 8 sec). During preconditioning (8 trials) and extinction (24 trials) acoustic startle probes (white noise bursts [50 ms; 95 dBA] were administered during and between slide presentation). During acquisition (16 trials) CS+ was reinforced by an electric shock. Startle response magnitudes significantly increased from preconditioning to extinction and were substantially larger to CS+. Conditioned startle reflex augmentation linearly increased with the pleasantness of the slides. Furthermore, subjects showed a greater post-conditioning increase of judged aversiveness to slides that they had previously reported to be more pleasant, exactly paralleling the startle reflex results.

Acquisition of the classically conditioned eyeblink response in humans over the life span.

Human subjects ranging in age from 18 to 85 years underwent classical conditioning of the eyeblink response to a tone conditioned stimulus (CS) and an air-puff unconditioned stimulus (UCS). There was a decline in percentage of conditioned responses with age. This decline was most noticeable in subjects over age 50. These conditioning deficits were not due to age-related changes in sensitivity to the tone CS or the air-puff UCS, nor could the conditioning deficits be attributed to an age-related decline in general cognitive abilities or to changes in spontaneous blink rates. The results are discussed in terms of using the classically conditioned eyeblink in humans in conjunction with the classically conditioned nictitating membrane response in rabbits as a model system for studying the neurobiology of age-related conditioning deficits.

Acquisition of the classically conditioned eyeblink response in humans over the life span.

Acquisition of the classically conditioned eyeblink response in humans over the life span.

Human operant eyelid conditioning: Auditory vs visual reinforcing signal

The human eyeblink response was conditioned as a free operant, employing a double-blind yoked-control procedure. High levels of conditioning were obtained with experimental Ss by employing either a visual or auditory reward signal. No significant differences in response level were associated with reward signal modality in conditioning nor, as previously hypothesized, for yoked controls. Evidence for a significant conditioning effect was obtained even when the data of only “unaware” Ss were considered.