The Eytex in vitro assay was used to evaluate 42 adult and baby shampoos. The assay, which is based on protein precipitation, was chosen because it provides rapid, quantitative results at a relatively low cost. All shampoos were diluted 1:10 in deionized water and tested with two different protocols: the rapid membrane assay (RMA) protocol for adult products and the high-sensitivity assay (HSA) for baby shampoos. All samples were tested in two and, in some cases, three separate experiments and the qualified Eytex scores were averaged. The correlation of the Draize scores with the Eytex scores was statistically significant (r2 = 0.90). The Eytex scores were used to establish irritation classes similar to the standard Draize classification scheme (minimal, mild, moderate, severe). When the Draize eye irritation class was compared to the Eytex irritation class for a given shampoo, the correlation for the baby shampoo data was 100% and 89% for the adult shampoo data. This study indicates that the Eytex in vitro assay for ocular irritancy can be highly predictive of Draize eye scores for shampoos. This suggests that such assays could be useful as screening tools in new shampoo development.