It is well known that a decline of arousal level causes of poor performance of movements or judgments. Our previous study indicates that microsaccade (MS) rates and pupil fluctuations change before slow eye movements (SEMs) (Honda et al. 2013). However, SEM detection of this study was obscure and insufficient. In this study, we propose a new SEM detection method and analyze MS rates and pupil fluctuations while subjects maintain their gaze on a target. We modified Shin et al.'s method, which is optimized for EOG (electrooculography) signals, to extract the period of sustaining SEMs using a general eye tracker. After SEM detection, we analyzed MS rates and pupil fluctuations prior to the initiation of SEMs. As a result, we were able to detect SEMs more precisely than in our previous study. Moreover, the results of eye movements and pupil fluctuations analyses show that gradual rise of MS rate and longitudinal miosis are observed prior to the initiation of SEMs, which is consistent with our previous study. These findings suggest that monitoring eye movements and pupil fluctuations may evaluate the arousal level more precisely. Further, we found that these tendencies become more significant when they are restricted to the initial SEMs.