Extremely Low Frequency Magnetic Fields Exposure Research Articles

Occupational exposure to electromagnetic fields from dental devices: A descriptive study

Abstract
 
 Objective: Electromagnetic Field sources which are widely used as part of the operating mechanism occupational exposure occurs with the electricity is generated, distributed or usage in the everyday environment. We aimed to obtain the Extremely Low Frequency-Magnetic Field exposure profile for dental health workers from electrical devices used in dentistry. 
 Methods: Measurements were performed while appliances under working conditions, 10 cm distance with the device for 6 minutes and for 7 days. The highest measured value in every 10 second was recorded. The mean ± SD of minimum and maximum Extremely Low Frequency-Magnetic Field for high-speed dental handpiece with LED, low-speed dental handpiece, model-trimming machine, automatic boil-out unit, steam machine, vacuum device, and polishing machine were recorded in milliGauss. 
 Results: The values which obtains from model-trimming machine and polishing machine were remarkable (809.17 ± 37.70 / 975.00 ± 10.27, 649.33 ± 201.37 / 1367.00 ± 32.17, respectively). Conclusion: The results show that periodic Electromagnetic Field exposure measurements should be conducted to obtain more detailed information about workplace exposures and sources.
 
 Keywords: Health, electromagnetic field, extremely low-frequency field, exposure, occupational

Can extremely low frequency magnetic field affect human sperm parameters and male fertility?

Exposure to extremely low frequency magnetic fields (ELF-MF) may have different effects on spermatozoa depending on the waveform, magnetic flux density, frequency of ELF-MF, and duration of exposure. In this study, we investigated the possible role of ELF-MF (50 Hz; 1 mT) exposure in altering sperm parameters. In this study we found that exposure to ELF-MF at the frequency of 50 Hz (1 mT) for two hours induces statistically significant alterations in progressive motility, morphology and reactive oxygen species (ROS) production of human spermatozoa, suggesting a role of ELF-MF in altering reproductive function of spermatozoa. Our results represent an important discovery in the field since occupational exposure to the sine waveform 1 mT 50 Hz ELF-MF used in our study is possible in workplace. Moreover, these electromagnetic fields are product by many electronic devices and household appliances. Thus, alterations of progressive motility and morphology of spermatozoa would be important consequences of human exposures to ELF-MF.

Proof of Concept of an ELF Magnetic Field Exposure System with Biphasic Magnetic Pulses: Effects on Human Dermal Fibroblast Proliferation

The aim of this work was to validate the usefulness of an Extremely Low Frequency (ELF) magnetic field exposure system with a magnetotherapy device of common use in clinical practice that generates Biphasic Magnetic Pulses (BMP), in order to study the effects of the BMP on the proliferation of Human Dermal Fibroblasts (HDF). In that regard, HDF were exposed 2h in the morning and 2h in the afternoon for 2 days to BMP of 1.41mT peak value at 5, 10, 25Hz continuous and 50Hz intermittent (2s on/ 1.5s off). MTT assay was performed to assess proliferation. The 10Hz BMP showed a significant decrease in proliferation of 6.6% (p = 0.001) with respect to controls, but no significant changes in proliferation were seen with the other BMP. In order to analyze whether these results could be related to the exposure protocol, a 50Hz power line intermittent signal (1s on/ 1s off) was generated and tested but exposure time was increased to 48h to cover the complete cells doubling time. A significant increase in proliferation of 9% (p < 0.001) was found in this case. The results validate the in vitro exposure system for its use with the BMP. Though the MTT proliferation assay alone is not enough to make definitive claims, the results might indicate that exposure time plays a key role in the outcome of the experiments. Therefore, special attention should be paid to the exposure time on in vitro protocols and how they relate to in vivo experiments and current treatments.

A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure

BackgroundThe exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF-10A breast cell line.Methods and resultsThe breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4–8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells.ConclusionsThe viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time.

ELF-MF Exposure, Actual and Perceived, and Associated Health Symptoms: A Case Study of an Office Building in Tel Aviv-Yafo, Israel

Empirical studies link exposure to extremely low frequency magnetic fields (ELF-MFs) to several health symptoms. However, it is unclear whether these symptoms are associated with actual or perceived exposure. In this study we attempted to answer this question by studying the health complaints of employees working in a multi-story office building located near a major high-voltage power line. ELF-MF measurements were conducted in the building using a triaxial sensor coil device on all 15 floors. In parallel, questionnaires were administered to evaluate the prevalence of various health symptoms among the employees. Multivariate logistic regressions were used next to quantify the associations between actual and perceived ELF-MF exposure and the employees’ health complaints. The analysis revealed that feelings of weakness, headache, frustration, and worry were associated with both measured and perceived ELF-MF exposure (p &lt; 0.01), while perceived ELF-MF exposure was also found to be associated with eye pain and irritation (OR = 1.4, 95% CI = 1.2–1.6), sleepiness (OR = 1.3, 95% CI = 1.1–1.5), dizziness and ear pain (OR = 1.2, 95% CI = 1.0–1.4). We conclude that high-voltage power lines produce both physiological and psychological effects in nearby workers, and, hence, proximity to such power lines should become a public health issue.

Effect of Short-time Exposure of Local Extremely Low-Frequency Magnetic Fields on Sleepiness in Male Rats.

Lack of high-quality sleep causes severe side effects like anxiety and changes in plasma concentration of oxalate. The current study investigated the impact of local extremely low-frequency magnetic fields (ELF-MFs) on inducing sleep (sleepiness) and anxiety in male rats. In this experimental study, 40 male rats were divided into four groups (n=10 for each group). The ELF-MF exposure (0, 10, and 18 Hz) was applied with an intensity of 200μT for three days (10 min/d). The sham-treated animal did not receive ELF-MF. Serum levels of oxalic acid (OA) and sleepiness were measured before and after the last exposure to ELF-MF or sham. Anxiety, sleepiness, and OA were measured using the elevated plus maze, open-field test (OFT), and ELISA test. A comparison of oxalate levels before and after exposure to ELF-MF revealed that ELF-MF (10 Hz) decreased the serum level of oxalate (P<0.05). Comparing open/closed arm entry (in an elevated plus maze) between before and after exposure to ELFMF revealed significant differences. Also, frequency, velocity, and distance moved were decreased in the open-field test. Results of the present study demonstrated that ELF-MF with short-time exposure may modulate the metabolism of OA and may modulate anxiety-like behavior or kind of induction of sleepiness in male rats. Oxalate acid concentration may reduce after short time ELF-MF exposure.Locomotor activity in male rats may decrease after the ELF-MF exposure.Short time ELF-MF exposure may induce sleepiness in male rats that may be used to treat sleep disorders. It is necessary for a person to have good sleep to feel happy during the day. The usual way to treat the patient's sleep disorders is drug therapy, but there are some non-pharmacological treatments such as cognitive behavioral therapy and proper diet. In this study we decided to evaluate the effect of ELF-MFs on sleep induction (sleepiness) in male rats by assessing behavioral tests and measuring oxalate acid density. The results showed that the activity of rats and oxalate acid concentration reduced after ELF-MF exposure. This was consistent with results of the plus maze test and the reduction of velocity, frequency and in the open-field test can be attributed to sleepiness. The results of this research showed that ELF-MF with short time exposure may modulate the anxiety-like behavior or kind of induction of sleepiness in male rats. This effect may be used to treat sleep disorders and requires further human studies.

Malignant lymphoma and occupational exposure to extremely low frequency magnetic fields and electrical shocks: a nested case-control study in a cohort of four Nordic countries

BackgroundExposure to extremely low frequency magnetic fields (ELF-MFs) and electric shocks is a common occupational risk factor in many workplaces. Recent investigations have highlighted a possible association between such exposures...

Extremely low frequency magnetic exposure attenuates oxidative stress and apoptotic cell death in injured spinal cord of rats

Secondary spinal cord injury (SCI) sets on immediately after trauma which results in vascular, morphological and biochemical changes at the site of lesion. Amongst these, the crucial events such as oxidative stress and apoptosis result in spreading the injury to adjacent tissues following the initial insult. Exposure to extremely low frequency magnetic field (ELFMF) is reported to modulate oxidative stress and cell death in vivo. Here, we investigated the influence of ELFMF on oxidative stress and cell death after SCI. Adult male Wistar rats were divided into Sham-SCI (Laminectomy only), Sham-SCI+MF (ELFMF exposure; 17.96 μT intensity, 50 Hz frequency, 2 h duration/day to Sham-SCI rats), SCI (complete transection of T13 spinal cord) and SCI+MF (ELFMF exposure to SCI rats) groups. They were sacrificed on day 2 for antioxidant assay and day 1, 2 and 3 for cell death study. The oxidative stress was assessed by antioxidant enzyme activities and the cell death was assessed by TUNEL assay and Caspase-3 immnuohistochemistry. Data revealed significant reduction in the lipid peroxidation and number of cells undergone cell death besides improvement in antioxidant levels in the spinal cord of ELFMF exposed SCI rats. These novel findings demonstrate the potential of ELFMF to attenuate oxidative stress and cell death in the early stage of secondary injury process after SCI.

Fermentation Process of Dry Cocoa Beans through Extremely Low Frequency (ELF) Magnetic Field Exposure

Several studies have found and proved that exposure to Extremely Low Frequency (ELF) magnetic fields can cause the proliferation of cells or bacteria. Meanwhile, bacteria play an essential part in the fermentation process. Thus, this study aims to examine the fermentation process of non-fermented dry cocoa beans using Extremely Low Frequency (ELF) magnetic field exposure. The sample of this study was 30 kg of non-fermented dry cocoa beans divided into 10 sample bags of 3kg each and grouped into 4. The control group (K) consisted of 1 bag while the rest bags were divided into 3 sample bags exposed to an ELF magnetic field with intensities of 100 μT (E-100), 200 μT (E-200), and 300 μT (E-300). ELF magnetic field exposure was carried out at the beginning of the fermentation process with variation in the exposure time of 15 , 45 , and 75 minutes. During the fermentation process, temperature measurements were conducted. Moreover, moisture content measurements were also conducted during the drying process in the sun for up to 3 days. At last, measurements of pH and alcohol content were carried out on dried cocoa beans. The findings showed that there was no significant temperature change (p &gt; 0.05) during the fermentation process. A decrease in the moisture content of cocoa beans during the drying process was proven to be faster in the sample group exposed to the ELF magnetic field compared to those in the control group. The alcohol and pH of dried cocoa beans from the sample group exposed to the ELF magnetic field were significantly higher than those in control (p &lt;0.05). The highest alcohol content was found in the sample group which was exposed to the intensity of the ELF of 200 μT for 45 minutes. Conclusion: Exposure to ELF magnetic fields can increase the fermentation activity of dry cocoa beans. This indicates that exposure to ELF magnetic fields in the fermentation process of cocoa beans might improve the quality of non-fermented dry cocoa beans.

Residential extremely low frequency magnetic fields and skin cancer

ObjectivePhotoinduced radical reactions have a fundamental role in skin cancer induced by ultraviolet radiation, and changes in radical reactions have also been proposed as a mechanism for the putative carcinogenic...

Investigating Bio-interface Effects of Chronic ELF-MF Exposure before and after Neonatal Life on Rat Offspring Using Spectroscopy and Biochemical Assays

Electromagnetic (EM) energy that generates extremely low-frequency magnetic fields (ELF-MF) impacts all living organisms' biological systems. To investigate the chronic impact of ELF-MF (50 Hz) we designed an externally applied electric field exposure to rat offspring during intrauterine and after extrauterine life. A total of 18 rat offsprings blood serum was analyzed via biochemical assays and ATR-FTIR (400 to 4000 cm-1) analysis. The changes in the samples' total antioxidants (TAS) and total oxidants level (TOS) were measured, and we calculated oxidative stress index (OSI) and TNF-α level in serum samples. To attribute diseases, chemometric models were validated using PCA-SVD analysis. Proteins, including the amide I (1631 cm−1), exhibit the strongest bands in the spectra depending on the secondary structure of the protein, and there was a degradation of lipids as a result of lipid peroxidation; also a significant increase in the lipid to protein ratio was concluded from the analysis (p&lt;0.001). Finally, EMF exposure statistically significantly interferes with lipids, protein, and DNA/RNA molecules. Our findings demonstrate that chronic EMF exposure is an environmental factor affecting blood serum parameters and could impair oxidant-antioxidant function, increase lipid peroxidation and oxidative stress depending on the continuity of ELF-MF exposure.

Exposure to 50 Hz Extremely-Low-Frequency Magnetic Fields Induces No DNA Damage in Cells by Gamma H2AX Technology.

The current results for extremely-low-frequency magnetic fields (ELF-MF) on DNA damage are still debated. A sensitive indicator and systematic research are needed to assess the effects of ELF-MF. In this study, we used γH2AX as an early and sensitive molecular marker to evaluate the DNA damage effects of ELF-MF in vitro. Human amnion epithelial cells (FLs), human skin fibroblast cells (HSFs), and human umbilical vein endothelial cells (HUVECs) were exposed to 50 Hz ELF-MF at 0.4, 1, and 2 mT for 15 min, 1 h, and 24 h, respectively. After exposure, cells were subjected to γH2AX immunofluorescence and western blot. The results showed no significant difference in the average number of foci per cell, the percentage of γH2AX foci-positive cells, or the expression of γH2AX between the sham and 50 Hz ELF-MF exposure groups (P > 0.05). In conclusion, 50 Hz ELF-MF did not induce DNA damage in FLs, HSFs, or HUVECs, which was independent of the intensity or duration of the exposure.

Extremely Low Frequency Magnetic Fields Increase the Expression of HIF-1 in Prostate Cancer Cells

Background: Increased levels of hypoxia-inducible factor 1α (HIF-1α) are related to poor prognosis and treatment failure in prostate cancer. The effect of extremely low frequency magnetic fields (ELF-MF), generally emitted from electronic devices on HIF-1α in cancer cells and, specifically, in prostate cancer is not well described. This study aimed to investigate the effects of ELF-MF on HIF- 1α protein expression and HIF-1 transcriptional activity in prostate cancer cells. Methods: Prostate cancer cells were irradiated with ELF-MF by means of a frequency generator at 875 MHz with an intensity of 0.07 mW/cm2 at various time periods and with an exposure system housed in a tissue culture incubator. The expression of HIF-1α protein and HIF-1 transcriptional activity at the different conditions were evaluated. Results: HIF-1α expression in different prostate cancer cells was higher after one hour of exposure to ELF-MF. All exposed cells also demonstrated a significant 50% increase in HIF-1 transcriptional activity as measured by an HRE-dependent reporter gene assay after ELF-MF irradiation. Cells exposed to ELF-MF exhibited 3-fold longer HIF-1α half-life compared to non-exposed control cells, while its mRNA levels were not affected. Immunofluorescence staining confirmed nuclear HIF-1α accumulation following exposure to ELF-MF. Cell proliferation was increased following ELF-MF exposure and significantly reduced when HIF-1α was silenced even after exposure to ELF-MF. Conclusions: Exposure of prostate cancer cells to ELF-MF increases HIF-1α protein stability and upregulates HIF-1 transcriptional activity. These findings provide new insights into the effects of ELF-MF on the hypoxic pathway which are important for guiding future implications.

Extremely Low-Frequency Magnetic Fields and the Risk of Childhood B-Lineage Acute Lymphoblastic Leukemia in a City With High Incidence of Leukemia and Elevated Exposure to ELF Magnetic Fields.

It is important to study the relationship between extremely low-frequency magnetic fields (ELF-MFs) and childhood leukemia, particularly in locations with a high incidence of this neoplasm in children and an elevated exposure to ELF-MF, such as Mexico City. The aim was to investigate the association between ELF-MF exposure and the risk of B-lineage acute lymphoblastic leukemia (B-ALL). A case-control study was conducted in Mexico City during the period from 2010 to 2011. Residential 24-h ELF-MF measurements were obtained for 290 incident B-ALL patients and 407 controls, aged less than 16 years. Controls were frequency-matched by sex, age (±18 months), and health institution. The adjusted odds ratios (aOR) and 95% confidence intervals (CIs) were calculated. ELF-MF exposure at <0.2 μT was used to define the reference group. ELF-MF exposure at ≥0.3 μT was observed in 11.3% of the controls. Different ELF-MF intensity cutoff values were used to define the highest exposure category; the highest exposure category for each cutoff value was associated with an increased risk of B-ALL compared with the corresponding lower exposure categories. The aORs were as follows: ≥0.2 μT = 1.26 (95% CI: 0.84-1.89); ≥0.3 μT = 1.53 (95% CI: 0.95-2.48); ≥0.4 μT = 1.87 (95% CI: 1.04-3.35); ≥0.5 μT = 1.80 (95% CI 0.95-3.44); ≥0.6 μT = 2.32 (95% CI: 1.10-4.93). ELF-MF exposure as a continuous variable (per 0.2 μT intervals) was associated with B-ALL risk (aOR = 1.06; 95% CI: 1.01-1.12). In the present study, the proportion of children exposed to ≥0.3 μT is among the highest reported worldwide. Additionally, an ELF-MF exposure ≥0.4 μT may be associated with the risk of B-ALL. Bioelectromagnetics. © 2020 Bioelectromagnetics Society.

Insights in the biology of extremely low-frequency magnetic fields exposure on human health.

The extremely low-frequency magnetic fields (ELF-EMF) are generated by electrical devices and power systems (1 to 300Hz). In recent decades, exposure to ELF-EMF has emerged potential concerns on public health. Here, we discuss recent progress in the understanding of ELF-EMF biology with a focus on mechanisms of ELF-EMF-mediated disease and summarize the results of more recent experimental and epidemiological studies of ELF-EMF exposure effects on cancer, neurological, cardiovascular, and reproductive disorders. Current views on genomic instability effects, as well as scientific evidence about ELF-EMF therapy, are put forth. According to our literature review, exposure to ELF-EMF has an adverse biological effect depending on the current intensity, strength of the magnetic field, and duration of exposure. Accumulated epidemiologic evidence indicates a correlation between exposure to ELF-EMF and childhood cancer incidence, Alzheimer's disease (AD), and miscarriage. However, adult cancer does not show augmented risk caused by the ELF-EMF. Also, no consistent evidence exists in cardiovascular disease mortality due to ELF-EMF exposure. There is a lack of comprehensive mechanisms for explaining the biological effect of ELF-EMF. Eventually, more studies are needed to clarify the mechanisms of these magnetic fields.

Optimizing lactobacillus growth in the fermentation process of artificial civet coffee using extremely- low frequency (ELF) magnetic field

This study aimed to analyze the fermentation process of Civet Coffee through Extremely Low Frequency (ELF) magnetic field radiation. The research method used a Completely Randomized Design (CRD) experimental. The sample of high quality pulped coffee that fermented by adding α- amylase and is grouped in seven. The six groups are exposed by ELF magnetic field in 100 μT and 300μT for 15 minutes, 30 minutes, and 45 minutes, and one group is served as the control. The result indicated that the population of Lactobacillus in the experiment groups increases significantly compared to the control. The exposed sample achieves the highest population in 300 μT intensity for 45 minutes. Also, the total of pH during the fermentation process did not show any significant changes. ELF magnetic field exposure in 300 μT optimized Lactobacillus proliferation drastically in the fermentation process of artificial Civet coffee. This research can contribute to developing fermentation technology based on ELF magnetic field radiation as well as improving the quality of artificial Civet coffee.

A potency of ELF magnetic field utilization to the process of milkfish preservation (chanos chanos)

This research aims to prove that Extremely Low Frequency (ELF) magnetic field radiation can be used in the process of milkfish preservation. The sampling consists of three groups, the first group is exposed by ELF magnetic field of 750 μT, the second group is exposed by ELF magnetic field of 900 μT, and these groups are exposed in 2x 30 minutes and 2x 45 minutes. The third group is the control, which does not need to be exposed by the ELF magnetic field. The research finding shows that the ELF magnetic field radiation exposure of 730-μT intensity during 2x30 minutes in the fresh milkfish has been proven to reduce bacterial growth up to 73% and the exposure of 880μT ELF magnetic field radiation can reduce the bacterial growth up to 62% in the fifth hour after the exposure than the control. Meanwhile, the ELF magnetic field exposure by the intensity of 730μT or 880μT during 2 x 45 minutes significantly shows the stability of bacteria number than the control. Therefore, the ELF magnetic field radiation in more than 700μT intensity in 2 x 30 minutes has the potential to be applied in the process of milkfish preservation.

Human Postural Responses to High Vestibular Specific Extremely Low-Frequency Magnetic Stimulations

Background: International agencies recognize the lack of knowledge to further establish standards and guidelines to protect the workers and the public from extremely low-frequency magnetic fields (ELF-MF). In that regard, postural control has been proposed as a biomarker of potential adverse effects in humans. Considering its crucial role in postural control and its specific neurophysiological characteristics, the vestibular system emerges as an ELF-MF likely target. However, postural modulation to vestibular ELF-MF exposure remains inconclusive. Previous studies led us to investigate stimulation orientation and point of application to clarify the ELF-MF impact on balance in humans. Objectives: This research aimed to investigate the acute postural impact of lateral vestibular-specific ELF-MF stimulations. Methods: Postural control of thirty eight healthy participants was analyzed with lateral vestibular-specific ELF-MF stimulations ranging from 20 Hz to 160 Hz, up to 142 T/s and vestibular electrical stimulations at the same frequencies. Both spatial orientation and quantity of movement variables were used to investigate postural modulations. Results: Despite a conclusive positive control effect, no significant effects of ELF-MF and alternating current stimulation exposures were found regardless of frequency conditions. Conclusions: Although important electric fields were generated, no postural modulation was found. However, at these frequencies, the potential vestibular activation did not translate into functional postural sway but might be observed with reflexive vestibular outcomes.

Human Postural Control Under High Levels of Extremely Low Frequency Magnetic Fields

Background: International agencies such as the International Commission on Non-Ionizing Radiation Protection (ICNIRP) and the International Committee on Electromagnetic Safety (ICES) of the Institute of Electrical and Electronics Engineers (IEEE) need further data to set international guidelines to protect workers and the public from potential adverse effects to Extremely Low-Frequency Magnetic Fields (ELF-MF). Interestingly, electromagnetic induction has been hypothesized to impact human vestibular function (i.e. through induced electric fields). To date, a theoretical 4 T/s vestibular threshold was proposed to modulate postural control, but data is lacking above this limit. Objectives: This research aimed to investigate the impact of full head homogeneous ELF-MF stimulations above the 4 T/s threshold on human postural control. Methods: Postural control of twenty healthy participants was analyzed while full head homogeneous ELF-MF stimulations (20 Hz, 60 Hz, and 90 Hz) up to 40 T/s were applied. Velocity, main direction and spatial dispersion of sway were used to investigate postural modulations. Results: Despite a conclusive positive control effect, no significant effects of ELF-MF exposures on velocity, spatial dispersion, and direction of the postural sway were found for our 3 frequency conditions. Conclusions: The homogeneous full head MF stimulations oriented vertically and delivered at high frequencies induced E-fields having a weaker impact than anticipated, possibly because they impacted only a small portion of the vestibular system. This resulted in an absence of effect on postural control outcomes.

Long-Term Monitoring of Extremely Low Frequency Magnetic Fields in Electric Vehicles

Extremely low frequency (ELF) magnetic field (MF) exposure in electric vehicles (EVs) has raised public concern for human health. There have been many studies evaluating magnetic field values in these vehicles. However, there has been no report on the temporal variation of the magnetic field in the cabin. This is the first study on the long-term monitoring of actual MFs in EVs. In the study, we measured the magnetic flux density (B) in three shared vehicles over a period of two years. The measurements were performed at the front and rear seats during acceleration and constant-speed driving modes. We found that the B amplitudes and the spectral components could be modified by replacing the components and the hubs, while regular checks or maintenance did not influence the B values in the vehicle. This observation highlights the necessity of regularly monitoring ELF MF in EVs, especially after major repairs or accidents, to protect car users from potentially excessive ELF MF exposure. These results should be considered in updates of the measurement standards. The ELF MF effect should also be taken into consideration in relevant epidemiological studies.