Abstract
Secondary spinal cord injury (SCI) sets on immediately after trauma which results in vascular, morphological and biochemical changes at the site of lesion. Amongst these, the crucial events such as oxidative stress and apoptosis result in spreading the injury to adjacent tissues following the initial insult. Exposure to extremely low frequency magnetic field (ELFMF) is reported to modulate oxidative stress and cell death in vivo. Here, we investigated the influence of ELFMF on oxidative stress and cell death after SCI. Adult male Wistar rats were divided into Sham-SCI (Laminectomy only), Sham-SCI+MF (ELFMF exposure; 17.96 μT intensity, 50 Hz frequency, 2 h duration/day to Sham-SCI rats), SCI (complete transection of T13 spinal cord) and SCI+MF (ELFMF exposure to SCI rats) groups. They were sacrificed on day 2 for antioxidant assay and day 1, 2 and 3 for cell death study. The oxidative stress was assessed by antioxidant enzyme activities and the cell death was assessed by TUNEL assay and Caspase-3 immnuohistochemistry. Data revealed significant reduction in the lipid peroxidation and number of cells undergone cell death besides improvement in antioxidant levels in the spinal cord of ELFMF exposed SCI rats. These novel findings demonstrate the potential of ELFMF to attenuate oxidative stress and cell death in the early stage of secondary injury process after SCI.
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