Pomegranate Extract Research Articles

Effect of Adding Aqueos Extract of Pomegranate (Punica Granatum) Peel and Moringa (Moringa Oleifer) Dried Leaves Powder to Drinking Water in Carcass Characteristics and Some Immunological and Microbial Traits of Broiler Chickens

Conducted this study in a field Poultry Birds, Animal Production Division -faculty of Agriculture-Karbala University, For the period 10/8/2020 until 30/11/2020, To find out Effect of adding aqueous extract of pomegranate peel powder and dried moringa leaves to drinking water in the Carcass traits and some immunological and microbial traits for broiler meat. used in this experiment210 broiler chicks of the breedRoss308 is not naturalize done day old, the chicks were fed on a starter ration for 1-14 days from the age of the bird and a rationa second15 -28nodding and a bush Final for 29-42 days, and a starting weight 40g/chick, and distributed birds distribution randomly on seven tretments Each transaction contained 30bird With three replications Each repeat contained 10 birds. If it includes two parameters: the first transaction T1 control treatment without any addition, second treatment T2 addition of aqueous extract to Pomegranate peels, at a concentration of 25 ml / liter, Third transaction T3 Addition of aqueous extract to Pomegranate peels, at a concentration of 37.5 ml / liter, fourth treatment T4 Addition of aqueous extract to Pomegranate peels, at a concentration of 50 ml / liter, the fifth treatment T5 Adding aqueous extract of moringa leaf powder at a concentration of 50 ml/L, sixth treatment T6 adding aqueous extract of moringa leaf powder at a concentration of 75 ml/L of water, seventh treatment T7 adding aqueous extract of moringa leaf powder at a concentration of 100 ml/L. Results are shown moral improvement (p≤0.05) in the characteristics of the carcass with a decrease in the logarithmic numbers in for bacteria E. colli, high morale in logarithmic numbers for bacteria Lactobacillus in the contents of the jejunum and the ileum Having a moral high (<0.05) in the volumetric standard of antibodies against Newcastle disease and in the relative weight of the Fabricia gland, the thymus gland, the spleen and the Fabricia index compared to the control treatment Tretments are recorded T4T6 the best results in all the studied traits.

Effect of Adding Aqueos Extract of Pomegranate (Punica Granatum) Peel and Moringa (Moringa Oleifera) Dried Leaves Powder and their Mixture to Drinking Water in Carcass Characteristics and Some Immunological Tarits of Broiler Chickens

Conducted this study in a field Poultry Birds, Animal Production Division-faculty of Agriculture-Karbala University for the period 3/27/2021 until 9/5/2021. To find out Effect of adding aqueous extract to pomegranate peel powder and leaves dried moringa and their combination in drinking water in characteristics of the carcass Some of the immunological characteristics for broiler used in this experiment180 broiler chicks of the breedRoss308 is not naturalized One day old The chicks were fed on a starter ration for 1-14 days from the age of the bird and a rationa second 15 -28 nodding and a bush Final for 29-42 days and a starting weight 41grumble/chick, and distributed bird distribution randomly on seven transactions Each transaction contained 30bird With three replications Each repeat contained 10 birdsIf the experimental parameters include the following: the first transactionT1 control treatment without any addition, second treatment T2 addition of aqueous extract to Pomegranate peels at a concentration of 50 ml / liter of water, the third treatmentT3 Addition of aqueous extract of moringa leaf powder at a concentration of 75 ml / liter of water, Fourth treatmentT4 add 75% aqueous extract of pomegranate peel powder Ml/liter with aqueous extract for powder Moringa leaves 25% ml/liter, Fifth treatment T5 Adding the aqueous extract of pomegranate peel powder at a concentration of 50% at a concentration (25 ml/L) with the aqueous extract of dried moringa leaf powder at a concentration of 50% at a concentration/L., Sixth treatment T6 add 25% aqueous extract of pomegranate peel powder With 75% aqueous extract of Moringa leaf powder. Results are shown moral improvement (p≤0.05) in carcass traits with morale high in the standard volumetric For antibodies directed against Newcastle disease and in the relative weight of the Fabricia gland, Thames, spleen and the Fabricia index. Compared to the control treatment Transactions are recorded T2T5 The best results in all the studied traits.

Isolation, Purification, and Characterization of Pecan Nut Lipase and Studying its Affinity towards Pomegranate Extracts and 1, 4-Diacetoxybenzene

This study included partial purification of the lipase enzyme from the karnel of the pecan nut. After applying ion exchange chromatography technique using CM-Cellulose, two lipase isoenzymes were observed with specific activity values of 2.66 and 1.7 units/mg protein. The highest activity of both isoenzymes appeared at the optimum pH values of 8 and 6 and at temperatures of 40 and 50 °C, respectively. A pure single band was obtained by using electrophoresis technique and it was found that the approximate molecular weight was 42 kDa for the two isoenzymes. The study dealt with the preparation of the diester compound 1,4-diacetoxybenzene, which was diagnosed by GC-MS and 1H NMR spectrometry. When studying the inhibition mode, the above compound showed a competitive inhibition of the lipase, as the value of Km increased from 0.013 to 2.857 mM, and Vmax was 0.25 enzymatic units / ml / min. Inhibition constant Ki was calculated and its value was 0.0319 mM. Pomegranate peel extracts with chloroform and ethyl acetate revealed an activating effect on lipase. This research aims to detect the presence of lipase in the pecan nut karnel and purify it by biochemical methods to know its properties and molecular weight. Then we attempted to inhibit it with a laboratory-prepared compound that has a structure similar to the basic substance on which the enzyme works.

Plant Extract and Essential Oil Application against Food-Borne Pathogens in Raw Pork Meat.

Herbal and plant extracts are being applied for a wide range of foods against different types of food-borne pathogens. In the present study, ethanolic and aqueous extracts (2% w/v) from cranberry (Vaccinium macrocarpon) and pomegranate (Punica granatum L.) plants were applied alone or in combination with two essential oils (thyme and oregano in a concentration of 0.150 μg/g) in pork meatballs and their antimicrobial activity was estimated. The extracts exhibited promising results (aqueous and ethanolic extracts of pomegranate and cranberry in a food-compatible concentration of 2% w/v) were applied to raw pork meatball production and their antimicrobial activity was recorded versus Enterobacteriaceae, total mesophilic bacteria, yeasts/molds, Staphylococcus spp., Pseudomonas spp. and lactic acid bacteria (LAB). The outcome demonstrated that meatballs containing aqueous extracts of pomegranate were more resistant to spoilage compared to all the other samples since they were preserved for more days. The chemical profiles of plant extracts were determined through LC-QTOF/MS and the chemical composition of the essential oils applied was determined with the use of GC/MS in order to identify the substances involved in the observed antimicrobial activity. Phenolic acids (quinic acid, chlorogenic acid), monoterpenes (p-cymene, carvacrol, thymol, limonene), organic acids (citric acid) and phenols were the main constituents found in the plant extracts and essential oils applied. These extracts of plant origin could be used as natural preservatives in meat products, even in low concentrations.

ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF ETHANOLIC EXTRACT OF LEAVES OF PUNICA GRANATUM L. ON EXPERIMENTAL ANIMAL MODELS

Objective: The aim of the study is to evaluate the analgesic and anti-inflammatory activities of the ethanolic extract of Punica granatum L. (EEPG) on experimental animal models. Methods: Tail-flick method was used to test the central analgesic activity, using Pethidine as standard drug. The tail flick latencies or the basal reaction time of the animals were assessed using an analgesiometer. Glacial acetic acid induced writhing response was used to test the peripheral analgesic activity, using Aspirin as standard drug. Number of writhing responses was counted for 20 min in each group and the percentage protection was calculated. And Carrageenan induced rat paw edema method was used to test anti-inflammatory activity of EEPG against acute inflammation, using Aspirin as standard drug. The inhibition of rat paw edema was calculated in percentage. Results: In central analgesic activity, the extract and pethidine showed significant increase in the reaction time. In peripheral analgesic activity, the extract and aspirin significantly reduced the number of writhes induced by acetic acid. And in anti-inflammatory activity, the extract produced significant reduction of the carrageenan induced paw edema. Conclusion: The EEPG has demonstrated significant analgesic and anti-inflammatory activity.

Preliminary studies of microencapsulation and anticancer activity of polyphenols extract from Punica granatum peels

AbstractThe purpose of this study was to prepare functional formulations using two different types of Punica granatum (pomegranate) peels extracts (the native and the enriched extract), as an important source of polyphenolic compounds such as punicalagin and ellagic acid. These two extracts were microencapsulated by a spray‐drying technique. Polyphenol constituents of P. granatum play a significant role in the prevention of different diseases from cancer to cardiovascular and neurodegenerative diseases. In general, polyphenols are sensitive and present low bioavailability in the human body. Microencapsulation could be a perfect alternative to modify the reactivity, durability, sensitivity, and photosensitivity of these natural compounds. Arabic gum, pectin, and modified chitosan were used as biopolymers‐based carriers in this research. The mean size of the microparticles prepared is between 2.55–6.86 μm (volume distribution). Release studies were implemented. The fastest release was observed for Arabic gum‐based carriers. The pectin‐based microparticles showed the slowest release profile. The Korsmeyer‐Peppas model was adjusted to the experimental release profiles. In addition, some anticancer activity studies were performed. When incubated with the human gastric cancer cell line AGS and human lung cancer cell line A549, both extracts elicited some loss of cancer cell viability, which increased in the case of the enriched extract. The bioactive pomegranate extracts microencapsulated seem to be a valid strategy to enhance their biological activity. A final powder formulation was obtained, which can improve the bioavailability and stability of these active constituents and overcome their limitations of application.

Effect of pomegranate and orange peel extracts on the quality of fish ham under frozen storage

Aim: The objective of the present study was to evaluate the effect of pomegranate and orange peel crude extract on fish ham quality in terms of biochemical, microbiological, and sensory parameters under cold storage (-18±2oC). Methodology: The crude extracts were extracted from dry powder of orange and pomegranate peel using absolute ethanol as solvents. The extracts were standardized in the mackerel cooked meat model system to determine the concentration for the final products. Fish ham was prepared using red meat of tuna (Thunnus albacares) and white meat of bull’s eye fish (Priacanthus hamrur). The prepared fish ham was treated with 2000 mg l-1 of pomegranate and orange peel extracts and stored under frozen conditions. Results: The results obtained were compared with a control sample containing synthetic antioxidant Butylated hydroxyanisole. The results showed that pomegranate and orange peel extracts significantly (p < 0.05) reduced the lipid oxidation and microbial activity. The results of biochemical studies revealed that there was a considerable increase in the quality parameter in all treated samples. The fish ham treated with pomegranate peel extract, orange peel extracts showed formation slower primary, secondary oxidation products and breakdown of nitrogenous compounds. Interpretation: Pomegranate and orange peel can be a substitute for synthetic antioxidants. These findings suggest that the pomegranate and orange peel serve as a choice to use as a food antioxidant for extending the shelf life of fish fishery products.

Comparative Evaluation of Antimicrobial Effects of Herbal Extracts Incorporated in Dentifrices among Adults: An In-vitro Study and a Double Blinded Randomized Control Trial

Introduction: A positive correlation has been observed between Streptococcus mutans, Candida albicans and periodontal diseases. Several substances including synthetic chemicals and natural agents have shown promising antimicrobial activity against the S. mutans- C. albicans complex. Extracts of Aloe Vera, Triphala, Tulsi, Pomegranate, and Acacia catechu have established therapeutic effects in medicine. The aim of the study was to evaluate the antimicrobial activity and the Minimum Inhibitory Concentration (MIC) of five herbal extracts in-vitro and to compare the antibacterial effects of 5 dentifrices containing the herbal extracts and a commercially available dentifrice on the plaque sample counts of S. mutans and C. albicans in-vivo
 Methodology: Extracts were obtained using a standardized method. Minimum inhibitory concentration was calculated for the test microorganisms using modified agar-well diffusion and macro broth dilution technique and the inhibition zone diameters were calculated. Dentifrices containing the extracts were formulated and the in vivo antimicrobial activity was evaluated by studying the CFU count from plaque samples of 420 participants. 
 Results: Highest antimicrobial activity against S Mutans and C albicans was shown by Acacia catechu and Tulsi respectively and least was by pomegranate. Mean reduction in microbial counts (CFU/ml) by inoculating nutrient broth from baseline to 60 days was highest in Acacia catechu and Triphala for S Mutans and C albicans respectively. All herbal extracts performed better than the commercially available toothpaste.
 Conclusion: Dentifrices with herbal extracts of Aloe Vera, Triphala, Tulsi, Pomegranate, and Acacia catechu could serve as an alternative to commercially available dentifrices for treating oral diseases whose causative organisms are S. mutans and C. albicans.

Analysis of structural and biomimetic characteristics of the green-synthesized Fe3O4 nanozyme from the fruit peel extract of Punica granatum

Analysis of structural and biomimetic characteristics of the green-synthesized Fe3O4 nanozyme from the fruit peel extract of Punica granatum

Phytochemical Investigation, Synthesis and Characterization of Iron Oxide Nanoparticles by Peel Extract of Punica Granatum

Iron oxide nanoparticles are gradually being studied for many applications in the field of medicine, photonics, laser, and biophysics. In this experiment, dark synthesis is used for obtaining iron oxide nanoparticles from Punica granatum fruit peel extract. Results confirmed that all synthesized iron oxide nanoparticles have a purity and high degree of crystallinity. The size of the nanoparticles which is synthesized by this root has a high degree of crystalline structure with a crystalline size of 37.71 nm. The highest peaks angle is 33.240, and 35.710 with the interplanar spacing (hkl) at (104), and (112) respectively, and the index hkl based on ICSD no. 01-079-0007. UV–vis absorption recorded a characteristic peak at 653.9 nm for iron oxide nanoparticles. The bandgap analysis at 1.81 eV.

Iron oxide nanoparticles synthesized using plant(Beta vulgaris and Punica granatum) extracts for a breast cancer cell line (MCF-7) cytotoxic assay

ABSTRACT We successfully developed a breast cancer cell line (MCF-7) cytotoxic assay using iron oxide nanoparticles (IONPs) prepared by mixing Beta vulgaris and Punica granatum extracts with iron chloride (III) salts at 200°C for 2 h using a simple chemical method. IONPs were diagnosed using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and photoluminescence (PL). XRD measurements exhibited a crystallite size of 38 and 37 nm with hexagonal and cubic structures for IONPs made using two plant (Beta vulgaris and Punica granatum) extracts, respectively. FE-SEM images revealed that the average IONP grain size was 44.81 and 29.77 nm for the two plant extracts, respectively, and PL measurements showed that the band edge emission was 2.64 and 2.60 eV, respectively, for IONPs. The IONPs were applied as a medical treatment for a human breast cancer cell line, and the inhibition rate% results were found to be relatively high and interesting (59.4% and 76.3%, respectively, for MCF-7).

Stabilization of PE with Pomegranate Extract: Contradictions and Possible Mechanisms.

Dry pomegranate peel was extracted with acetone and the extract was added to a Phillips type polyethylene. The concentration of the extract was changed from 0 to 1000 ppm in six steps and stabilization efficiency was checked by the multiple extrusion of the polymer followed by the characterization of chemical structure, processing, and residual stability. The results confirmed the excellent processing stabilization efficiency of the extract, but also the poor long-term stability of PE containing it in accordance with previously published results. The extract is amorphous and its solubility is relatively large in the polymer; thus, these factors cannot be the reason for the poor stabilization efficiency in an oxygen-rich environment. Chemical factors like the self-interaction of the polyphenol molecules, the stability of the radicals forming after hydrogen abstraction, and the lack of hydrogens with the necessary reactivity must be considered during the evaluation of the efficiency of the extract. These factors as well as the insufficient number of active hydrogens hinder the reaction of the additive molecules with oxygen-centered radicals, thus leading to inferior long-term stability. The extract can be used for the processing stabilization of polymers, but for applications requiring long-term stability, it must be combined with other natural antioxidants like flavonoids or Vitamin E.

Green Synthesis of Gold, Iron and Selenium Nanoparticles Using Phytoconstituents: Preliminary Evaluation of Antioxidant and Biocompatibility Potential.

This study aimed at fabricating gold (Au), iron (Fe) and selenium (Se) nanoparticles (NPs) using various natural plant extracts from the Fertile Crescent area and evaluating their potential application as antioxidant and biocompatible agents to be used in the pharmaceutical field, especially in drug delivery. The Au-NPs were synthesized using Ephedra alata and Pistacia lentiscus extracts, whereas the Fe-NPs and Se-NPs were synthesized using peel, fruit and seed extracts of Punica granatum. The phytofabricated NPs were characterized by the UV-visible spectroscopy, scanning electron microscope, Fourier transform infrared spectroscopy, X-ray diffraction (XRD) and energy-dispersive X-ray (EDS) spectroscopy. Scanning electron microscope technique showed that the synthesized NPs surface was spherical, and the particle size analysis confirmed a particle size of 50 nm. The crystalline nature of the NPs was confirmed by the XRD analysis. All synthesized NPs were found to be biocompatible in the fibroblast and human erythroleukemic cell lines. Se-NPs showed a dose-dependent antitumor activity as evidenced from the experimental results with breast cancer (MCF-7) cells. A dose-dependent, free-radical scavenging effect of the Au-NPs and Se-NPs was observed in the DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay, with the highest effect recorded for Au-NPs.

Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism.

Polyphenols show a spectrum of bioactive effects, including an influence on lipid metabolism. In this study, we performed activity-guided fractionations of black chokeberry (aronia), cranberry, and pomegranate extracts to identify the biologically active compounds. The extracts were prepared from fruit juice concentrates with the adsorbent resin Amberlite XAD-7 and were separated into a copigment and an anthocyanin fraction, followed by fractionation into a polymer and monomeric fraction by means of hexane precipitation. For further fractionation of the cranberry and pomegranate copigment fractions, high-performance countercurrent chromatography (HPCCC) was used. The compounds in each fraction were identified by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS), and the quantification was performed by ultra high-performance liquid chromatography-diode array detector (UHPLC-DAD) analyses. Each of the (sub-)fractions was tested in three in vitro assays: phosphodiesterase 3B (PDE) activity, lipid accumulation, and lipolysis in 3T3-L1 cells. The results showed that various fractions and subfractions can inhibit lipid accumulation and PDE activity as well as increase lipolysis, particularly copigments. Overall, our results indicate an influence of polyphenol-rich (sub-)fractions on the lipid metabolism.

Multivariate assessment for predicting antioxidant activity from clove and pomegranate extracts by MCR-ALS and PLS models combined to IR spectroscopy

This study evaluated the feasibility of infrared (MIR/NIR) spectroscopy coupled to chemometrics as an alternative method for determining the antioxidant activity (AA%) of pomegranate (Punica granatum) and clove (Syzygium aromaticum) alcoholic extracts versus the conventional DPPH method. Multivariate curve resolution with alternating least squares (MCR-ALS) and Partial least squares (PLS) regression were efficient to predict the AA%, thus providing good accuracy and low residuals compared to the standard method. The MCR-ALS combined with NIR data stood out among the other models with R2 ≥ 0.962 and RMSEP ≤ 3.38 %; furthermore, this technique presents the great feature of recovering the pure spectral profile of the analytes and identifying interferents in the sample. The application of chemometrics tools to predict the antioxidant activity of natural extracts resulted in a greener, low-cost and efficient process for the food industry.

BACTERIAL INHIBITORY EFFECT OF ALGERIAN POMEGRANATE (Punica Granatum L.) EXTRACTS (PEEL, JUICE, AND SEED) AGAINST MULTIDRUG RESISTANT BACTERIA

Punica granatum L. (pomegranate) is known popularly by its nutritional values and healthy benefits. The current study is aimed the evaluation of antibacterial effects of Punica granatum fruit extracts that cultivated in Algeria against environmental and clinical isolates of β-lactamase producing methicillin resistant Staphylococcus aureus (MRSA) and extended-spectrum beta-lactamase ESBL-producing Enterobacteriaceae by using agar diffusion methods and minimum inhibitory concentration (MIC)-determination. Moreover, the extracts were investigated for their phenolic content by the Folin-Ciocalteu method. The quantitative assays of total polyphenols revealed a richness of the different pomegranate extract in polyphenols, peel and seed ethanol extracts contain the highest values (880.06 ± 11.2 mg GAE/100 g and 1160.1 ± 45.20 mg GAE/100 g, respectively). Flavonoid contents were high in both of acetone, ethanol extracts of peel, followed by ethanol, and acetone extracts of juice. According to the disc diffusion data, all phenolic extracts had a bacterial inhibitory effect against all extended-spectrum beta-lactamase ESBL-producing Enterobacteriaceae with a strong inhibitor with MIC 512 g/mL strength. The pericarp extract is particularly effective against Escherichia coli, Citrobacter freundii, and Klebseilla pneumoniae. However, the extract Punica granatum (pericarp and juice) revealed broad-spectrum antibacterial activity with an inhibitory diameter zone size of 11 0.9 mm to 29 1.12 mm against β-lactamase generating methicillin resistant Staphylococcus aureus (MRSA) species.These results were similar in both of agar diffusion and MIC methods. From the above study, it can be concluded that Algerian pomegranate extracts possess remarkable antibacterial activity. This result could be related to the presence of bioactive metabolites in this fruit. Therefore, intensive future studies should be performed to characterize the antibacterial components that are behind for this biological property.

Potential of Polyherbal Formulation in Burn Wound Model

Polyherbal formula has been used everywhere in the global because of its medicinal and healing application. It has additionally referred to as polyherbal therapy or herb-herb combination. In an age of integrated healthcare where Ayush therapies are being accepted along side the fashionable medicine, we see that these are separate compartments in theory and practice. Wound healing begins at the time of injury and can last for a variety of times depending on the severity of the wound. The wound healing process can be divided into three stages: the inflammatory phase, the proliferative phase, and finally the remodelling phase, which determines the strength and appearance of the healed tissues. In the present work an attempt had been made to evaluate wound healing potential of Polyherbal formulation contains Punica granatum and Coleus aromaticus extract in burn wound model.

Chemical Characterization and Preliminary Evaluation of the Efficacy and Tolerability of a Food Supplement Based on Pomegranate Extract, B Vitamins, and Vitamin C against Prolonged Fatigue in Healthy Consumers

Fatigue, characterized by periods of exhaustion that interfere with normal activities and negatively affect quality of life, can be classified as prolonged fatigue (or short-term fatigue, SF) or chronic fatigue. Vitamin deficiency and chronic inflammation seem to be possible causes of fatigue. Due to the unavailability of effective remedies that attenuate fatigue and provide consumer satisfaction, the aim of this investigation is to assess whether a commercial food supplement consisting of pomegranate extract with known anti-inflammatory activity, B vitamins, and vitamin C can help fight SF. To this end, the chemical composition of whole fruit pomegranate dry extract was determined through a RP-UHPLC–HRMS method, which indicated the presence of ellagitannins, gallotannins, and organic and phenolic acids. In a survey of consumers of food supplements aimed at reducing fatigue, 78 subjects (21 men and 57 women) were recruited for one month to evaluate the efficacy and tolerability of this food supplement through validated questionnaires for assessing fatigue level and quality of life. The consumers reported a significant improvement in their conditions without adverse effects over this time period. Although further studies are needed, these preliminary data suggest the capacity of a combination of pomegranate extract and hydrosoluble vitamins to alleviate SF.

Reduction of some viral protein's gene expression of recombinant influenza A/H1N1-PR8 virus upon treatment with Punica granatum crude extract

BackgroundIt was we known that Flu infections have a place with the class Orthomyxoviridae furthermore, three distinct sorts (A, B and C sort infections) are known. Among these, influenza viral infections display the broadest host range (birds, people, and different well evolved creatures) also, can develop into profoundly pathogenic strains. So, the scientists tried to find a way to restrict this invasion which can cause certain death to human raise. Subjects & methodsDifferent concentrations of Punica granatum extract (2.5, 5, 10 and 20 μg/ml) prepared from the stock solution were used against human influenza A/Puerto Rico/8/34 H1N1 (IAV/PR8) to test its ability to prevent the virus to grow on MDCKII and A549 cells to check the effect of this plant extract on viral replication titter using plaque assay, gene expression of some viral proteins like non-structural protein NS1, nucleoprotein NP and Protein basic PB1 using reverse transcriptase quantitative real time polymerase chain reaction. Also, localization of NP protein was checked using immune-florescent staining technique. ResultsOur finding showed that there was significant decrease in IAV/PR8 ability to grow and replicate in MDCKII cells at multiplicity of infection (MOI) in both of 0.1 and 0.01 MOI. this finding was confirmed upon checking of expression of NS1, PB1 and NP proteins of IAV/PR8 that were reduced significantly at 10 and 20 μg/ml. Interestingly the localization of viral NP protein affected negatively upon the treatment of cells with elected concentration of plant extract by the weak localization outside the nucleus. ConclusionMethanolic extract of Punica granatum produced a strong anti-viral activity against IAV/PR8 replication ability and that was due to the severe effect on viral proteins expression especially on PB1 and NP which responsible for viral protein synthesis and NS1 protein which is a strong IFN antagonistic protein thus; weakening its replication ability in different cell lines.

Environmental-friendly extraction of Peepal (Ficus Religiosa) bark-based reddish brown tannin natural dye for silk coloration.

The present study aims to extract a natural reddish brown colorant from Peepal (Ficus religiosa) for silk dyeing using the microwave radiation process (MW). The colorant was isolated in aqueous and acidic media, and MW treatment for 1, 2, 3, 4, and 5min has been given to both fabric and extract to observe changes in color intensity. The dye variables have been optimized, and for sustainable shade making process with good fastness, 1.0-5.0g/100mL of sustainable chemical and bio-mordants has been employed. It has been found that after microwave treatment for 3min, under selected conditions, the irradiated aqueous extract has given high color intensity onto silk fabric. The utilization of 3% of Al, 4% of Fe, and 2% of tannic acid (T.A.) as pre chemical mordant whereas 4% of Al, 4% of Fe, and 3% of tannic acid as post chemical mordant have given good color characteristics. In comparison, 4% of acacia and 3% of turmeric and pomegranate while 3% of acacia and turmeric and 4% of pomegranate extracts as post-bio-mordant have given excellent color characteristics. It is concluded that MW treatment has an excellent sustainable efficacy to isolate colorant from Peepal bark for silk dyeing, whereas the inclusion of bio-mordants has not only made the process more sustainable and environmental friendly but also best K/S, and L*a*b* values have been acquired.