Moringa Oleifera Extract Research Articles

EXPLORING THE POTENTIAL OF MORINGA OLEIFERA EXTRACT AS A NATURAL TISSUE FIXATIVE FOR HISTOPATHOLOGICAL APPLICATIONS

Histopathology, crucial for disease diagnosis, heavily relies on effective tissue fixation to maintain cellular architecture. However, the carcinogenic nature of formalin has spurred interest in safer alternatives. Moringa oleifera, renowned for its medicinal properties, presents a promising natural fixative candidate. This study aimed to assess Moringa oleifera extract's viability as a fixative for histopathological applications, specifically focusing on its compatibility with subsequent histological analyses. Distilled water and normal saline solutions of Moringa extract were prepared at varying concentrations. Kidney tissue samples from euthanized rats were treated with these solutions alongside formalin. Tissue sections underwent manual processing, embedding, sectioning, and staining using Hematoxylin and Eosin (H&E) technique. Microscopic examination revealed varying degrees of tissue preservation across Moringa concentrations and solvents. Higher concentrations (15%) in distilled water exhibited optimal preservation, akin to formalin-fixed tissues, while lower concentrations and normal saline solutions showed suboptimal fixation. These findings highlight Moringa oleifera's potential as a tissue fixative, particularly at higher concentrations in distilled water. Further research is recommended to isolate active components and explore alternative Moringa extracts for enhanced tissue preservation. Comparative studies would elucidate the efficacy of Moringa-derived fixatives and inform their utilization in histopathological practice.

Investigating the Anticancer Properties of the Essential Oil and Aqueous Extract of Moringa oleifera and its Biosynthesized Metal Nanoparticles on MCF-7 and BT-549 Cell Lines

Investigating the Anticancer Properties of the Essential Oil and Aqueous Extract of Moringa oleifera and its Biosynthesized Metal Nanoparticles on MCF-7 and BT-549 Cell Lines

Microstructures and Dielectric Permittivity Properties of Fe3O4/Cdots Nanocomposites Synthesized by Green Route Utilizing Moringa Oleifera Extract and Watermelon Peel

Dielectric materials are beneficial for storing electrical energy due to their insulating and polarization properties in response to external electric fields. Magnetite has shown promise as a dielectric material among other materials due to its good magnetic properties, low toxicity, and biocompatibility. However, the weakness of Fe3O4, which has low stability and easy agglomeration, requires a modification on its surface by using Carbon dots (Cdots). This research investigates the dielectric properties of Fe3O4/Cdots obtained through the green synthesis method. Fe3O4 nanoparticles were synthesized using the co-precipitation method with Moringa oleifera leaf extract as a reducing and stabilizing agent. In contrast, Cdots were synthesized using the hydrothermal method with watermelon peel waste as a carbon source. The Fe3O4 composite nanoparticles were characterized using X-ray diffraction (XRD), scanning electron microscopy-energy dispersive X-ray (SEM-EDX), ultraviolet-visible spectroscopy (UV-Vis), and impedance spectroscopy. The XRD spectra revealed the existence of cubic inverse spinel and a reduction in crystal size as the concentration of Cdots increased, measuring 7.8 and 7.1 nm, respectively. SEM-EDX revealed that the sample is composed of Fe, O, and C elements and has a spherical shape with Cdots distributed on the surface of Fe3O4. The UV-Vis spectrum showed the absorption peak of Cdots at 282 nm. The Fe3O4 absorption peak is identical to the Fe3O4/Cdots absorption peak at 193 nm. The increase in band gap energy from 2.96 to 3.33 eV is related to the increase in Cdots concentration. In the 10-900 kHz frequency range, dielectric property tests demonstrated peak dielectric permittivity values (real and imaginary). A substantial decrease was observed between 10 kHz and 200 kHz, followed by a relatively stable pattern up to 900 kHz. The loss tangent value obtained has a tanδ value <0.5, which means that the addition of Cdots affects reducing the energy loss stored in Fe3O4.

Leaf Extracts of Moringa oleifera Cultivated in Baghdad: Characterization and Antimicrobial Potential against Endodontic Pathogens.

The use of medicinal plant preparations to clean and disinfect root canal infection is gaining popularity. The aim of this study was to evaluate the bioactive composition of leaf extracts of Moringa oleifera plants cultivated in Iraq (specifically Baghdad) and their antimicrobial activity against selected root canal pathogens for potential application in endodontic treatment. Materials and Methods. Moringa leaf extracts were prepared either through cold maceration or warm digestion techniques to perform an ethanolic or aqueous extraction, respectively. Phytochemical detection was performed before thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) to measure flavonoids and phenolic compounds within both extracts. Then, their antimicrobial activities were investigated against Streptococcus mutans, Enterococcus faecalis, and Candida albicans through minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), and agar well diffusion assay in comparison to NaOCl and Ca(OH)2. Results. Phytochemical screening showed several active ingredients but with higher expression of flavonoids and phenolic compounds. Also, different types of these compounds were detected through TLC and quantified by HPLC. MIC values for ethanolic extract against Streptococcus mutans, Enterococcus faecalis, and Candida albicans were 60, 65, and 55, respectively, while for aqueous extract, MIC values were 70, 80, and 50, respectively. Aqueous extract showed a higher inhibition zone than ethanolic extract for both Streptococcus mutans and Enterococcus faecalis with a statistically significant difference (p ≤ 0.001) for all tested materials except with NaOCl and Ca(OH)2 in Streptococcus mutans and Enterococcus faecalis, respectively. The ethanolic extract showed a higher inhibition zone against Candida albicans, with a statistically significant difference (p ≤ 0.001) for all tested materials. Conclusion. Ethanolic and aqueous extracts of Moringa oleifera leaves cultivated in Baghdad contain considerable quantities of phytochemicals, especially flavonoid and phenolic compounds, and demonstrated antimicrobial activities against selected endodontic pathogens. Therefore, Moringa leaf extracts could be suggested as an alternative antimicrobial material in endodontic treatment.

Green Synthesis of Magnetite Nanoparticles Using &lt;i&gt;Moringa oleifera&lt;/i&gt; and their Electro-Optic Surface Plasmon Resonance Properties

The application of an external electric field to the surface plasmon resonance (SPR) system of green-synthesized magnetite (Fe3O4) nanoparticles (MNPs) is very promising for increasing the SPR detection signal. Electro-optic surface plasmon resonance (EOSPR) behavior of MNPs has been successfully carried out. The EOSPR system was investigated using the Kretschmann configuration with the prism/Au thin film/MNPs/air layer arrangement and applying an electric voltage of 0 V, 2 V, 4 V, and 6 V. In this study, we synthesized MNPs using the green synthesis approach from moringa oleifera extract. The benefits of green synthesis include being safe, affordable, clean, and ecologically friendly processes. X-ray diffraction results obtained crystal size of the MNPs is about 9.2 nm with inverse spinel face-centered cubic crystal structure. Fourier transforms infrared characterization showed the presence of Fe-O bonds at wave numbers 569 cm-1 and 629 cm-1, indicating that MNPs were successfully formed. The saturation magnetization of the samples is 55.3 emu/g. The SPR angle of the SPR system Prism/Au thin film/air without the addition of MNPs is 44.66°. After being deposited by MNPs and induced by a voltage of 0 V, 2 V, 4 V, and 6 V, the SPR angles changed to 44.87°, 44.90°, 44.95° and 45.12°. The addition of MNPs and an external electric field causes the SPR angle to increase. The results of this study can provide new insights into the development of optical devices that can be manipulated electrically and have the potential for future biosensor applications.

Biopolymer zein nanoparticles loaded with Moringa Oleifera extract for improved wound healing activity: Development, Qbd based optimization and in vivo study

Recently, nanobiomaterials have been explored for improved biological activities and value addition to the herbal extracts. Zein is a natural biopolymer with excellent pharmaceutical characteristics for topical applications. Moringa oleifera plant possesses large number of phytopharmaceuticals and its leaves are used in wound healing since ancient time. In this study, we studied first time, encapsulation of Moringa oleifera leaves aqueous extract into zein nanoparticles which are developed and optimized using quality by design approach. Moringa oleifera leaves aqueous extract was characterized by FTIR and total phenolic content determination. Moringa oleifera leaves aqueous extract loaded zein nanoparticles were systematically characterized for particle size, PDI, zeta potential, transmission electron microscopy and loading capacity. Further, a gel having optimized formulation for topical application was prepared and characterized for pH, spreadability, extrudability and storage stability. The so developed formulation were tested for wound healing activities on animals and results clearly indicated significant activity (p < 0.05) in case of Moringa oleifera leaves aqueous extract loaded zein nanoparticles formulation than control and relatively improved wound healing than Moringa oleifera leaves aqueous extract only loaded gel . This study opens up new possibilities in exploring zein nanoparticles for herbal extract based therapeutic applications.

Green synthesis and characterizations of magnetic iron oxide nanoparticles using Moringa oleifera extract for improved performance in dye-sensitized solar cell

In this study, Moringa oleifera extract was used for the green synthesis of magnetic Iron Oxide (Fe3O4) nanoparticle. Natural dye from air-dried Carica papaya leaves was used as sensitizer along with commercially obtainable N719 Ruthenium dye. The optical absorptions between 350 and 650 nm confirmed the formation of the nanoparticle. After adding the nanoparticle to the TiO2 and loading it with dye, the absorbance in the visible range dramatically increased. The formation and stabilization of the nanoparticle may have been aided by the functional groups, according to the FTIR results. With an average crystallite size of 35.13 nm, the XRD patterns demonstrated the nanoparticle's crystalline nature. The morphologies of the nanoparticle demonstrated that it was well-formed and homogeneously dispersed. The presence of iron and other component elements was confirmed by the EDX spectra. The production of 40.83 nm-sized nanoparticles on average was further verified by the TEM micrograph, and the samples' crystallographic planes were identified by the SAED patterns. The photo-electrochemical performance of the DSSCs showed that the efficiencies increased significantly with percentage increase of 90.1% and 89.3% for Carica papaya and Ruthenium dyes, respectively. The green-synthesized Fe3O4 nanoparticle exhibited good properties for application in DSSC.

Sonochemically assisted fabrication of highly photoactive novel ZnO/MoO3 binary nanotubes anchored with Bi2O3 nanosheet for solar light-driven photocatalytic activity

In this work, we present the fabrication of a direct Z-scheme photocatalyst, ZnO/MoO3/Bi2O3 by using a sonochemical-assisted biochemical (leaf extract of Moringa oleifera) process. The structural, surface and morphological characteristics of the manufactured composite were comprehensively studied by using various techniques including UV–Visible spectroscopy, X-ray diffraction, FTIR, SEM/EDX analysis, HRTEM imaging, XPS for chemical composition investigation, and BET surface area analysis. Our findings reveal that the nanotubes of ZnO/MoO3 are exquisitely aligned and interconnected well with Bi2O3 sheets which can be ascribed to the effective prevention of photoinduced electron-hole (e−-h+) pairs recombination. Moreover, the ternary hybrid possesses low band gap energy of 2.26 eV which suggest that sensitivity toward visible light and a large surface area, thereby increasing the active sites on its surface. ZnO/MoO3 with Bi2O3 enhances interfacial electron translocation efficiency, leading to a slower recombination rate of hole-electron pairs. Consequently, the one-pot combinative synthesis demonstrates a synergistic effect on the separation rate of active charge carriers, resulting in improved production and transmission of photo-excited electrons consequently enhancing its photocatalytic activity. In contrast to ZnO, MoO3, Bi2O3 and ZnO/MoO3, the ZnO/MoO3/Bi2O3 composite exhibited an impressive 98.8 % degradation efficiency of malachite green (MG) dye within 48 min under direct sunlight radiation, under optimized conditions (pH 13, 75 mg photocatalyst dose). The photocatalytic degradation process exhibited a rate constant of 0.090 min−1, following first-order reaction kinetics. Additionally, inhibitor studies allowed us to propose a mechanism that highlights the significant contributions of h+ (holes) and •O2− in the photocatalytic process.

Effect of selected weeds and organic emulsions on seed germination, seedling growth and biochemical content in rice (Oryza sativa)

An experiment was conducted during 2021–22 at ICAR-National Institute of Biotic Stress Management, Raipur, Chhattisgarh, to understand the allelopathic impact of 4 selected weeds and 2 organic products on germination, seedling growth and biochemical content in rice in laboratory condition in a completely randomized design (CRD). The results indicate that 15% solution of Ascophyllum nodosum extract (T15) and 15% solution of Medicago denticulate extract (T18), inhibits the germination by 78.72 and 40.42%, respectively and 5% solution of Moringa oleifera extract (T4), 5% solution of Parthenium hysterophorus extract (T1) and 15% solution of vermiwash (T9) increased the germination by 25.53, 23.41, and 21.28% respectively as compared to control. Treatment T4 and T9 increased the seedling’s vigour by 112.8 and 109.08%, while among them treatments, viz. 15% solution of A. nodosum extract (T15) and 15% solution of M. denticulate extract (T18) decreased the vigourness by -88.60% and -59.05% respectively. Treatment T4 and 5% solution of vermiwash (T7) increased the biochemical content by 17.79% and 13.49% respectively, while 15% solution of panchgavya (T2), and treatment T15 were significantly decreased the biochemical content by 39.26 and 42.33% respectively. Based on findings, it may be concluded that negative correlation was found between the response of rice crop and increasing concentrations of weed extracts and panchgavya unlike vermiwash. But lower concentration of M. oleifera and vermiwash showed most positive impact on rice seedlings.

Efficacy of Hot Tea Infusion vs. Ethanolic Extract of Moringa oleifera for the Simultaneous Treatment of Nonalcoholic Fatty Liver, Hyperlipidemia, and Hyperglycemia in a Murine Model Fed with a High-Fat Diet.

Moringa oleifera (MO) is a native tree of Asia and is cultivated in some areas of Mexico as part of traditional horticulture. The aim of the present study was to compare the efficacy of MO infusion vs. MO ethanolic extract for the simultaneous treatment of nonalcoholic fatty liver (NAFLD), hyperlipidemia, and hyperglycemia in a murine model fed with a high-fat diet (HFD). BALB/c mice were fed a balanced diet (healthy control) or an HFD for 6 months. With this, the NAFLD model was established before starting a therapeutic intervention with MO for two months. The phytochemical analysis by nuclear magnetic resonance in 1H and 13C experiments showed signals for pyrrole alkaloids and triterpenes as the main constituents of the extract and infusion preparation. A significant reduction of SGPT, SGOT, lipids, urea, and glucose in blood among NAFLD groups treated with MO (infusion or extract) was found, when compared to the NAFLD-placebo group. Steatosis and liver inflammation were found to be decreased in the MO groups, as infusion or ethanolic extract. Infusion produced a better therapeutic effect than the extract in all parameters, except glycemic control, where the extract was better. As an additional finding, it is noteworthy that treatment with MO, particularly through infusion, resulted in improved motor activity. Moreover, a reduction in anxiety-like behavior was observed exclusively with the administration of infusion. These observations provide valuable insights into the potential broader effects of Moringa oleifera beyond the primary aim of the study.

DERAJAT APOPTOSIS SEL GANGLION RETINA PASCA PEMBERIAN TAMBAHAN EKSTRAK MORINGA OLEIFERA ORAL DIBANDINGKAN DENGAN TERAPI STANDAR Studi Eksperimental pada Tikus Wistar Model Methanol Toxic Optic Neuropathy

Abstract&#x0D; Introduction &amp; Objectives : Methanol toxic optic neuropathy can lead to retinal ganglion cell apoptosis which causes visual disturbance. Moringa oleifera has been reported to have an anti-inflamatorry effect that can prevent inflammatory mediators from being relased in methanol toxic optic neuropathy&#x0D; Methods : Twenty-one Wistar methanol toxic optic neuropathy were divided into 3 groups, namely base line group, control group that given intravenous methylprednisolone 4.5mg/6 hours for 3 days, and given standard theraphy and additional administration of oral Moringa oleifera 300mg/kgbodyweight/day for 4 weeks. Assessment of the degree of apoptosis by Anatomical Pathology with histopatolocigal scaoring. The study used Mann Whitney comparative hypothesis test (Significant p&lt;0.05)&#x0D; Results : The degree of retinal ganglion cell apoptosis was significantly lower in the treatment and standard therapy groups compared to the base line group. The treatment group compared to the standard therapy group was not significantly different (p = 1,000)&#x0D; Conclusion : The degree of retinal ganglion cell apoptosis in rat model methanol toxic optic neuropathy was not significantly different between the group that was given additional oral Moringa oleifera extract compared to the standard theraphy group

Evaluation of Antifungal Activity of Plant Extracts against Papaya Anthracnose (Colletotrichum gloeosporioides) under In-vitro Condition

The study aimed to assess the effectiveness of various plant extracts against Colletotrichum gloeosporioides in papaya. This investigation employed a completely randomized design and took place at the College of Agriculture in Pune-05 during the academic year 2021-22.Among the six plant extracts tested, garlic (Allium sativum) was found to be superior to the rest, exhibiting the minimum mycelium growth (5.30 mm) and the highest percentage inhibition (94.11%) at a 20% concentration. However, the drumstick leaf (Moringa oleifera) extract was the least effective, with the maximum radial mycelial growth of 44.00 mm (51.11% inhibition) observed. The study indicates that the radial mycelial growth of Colletotrichum gloeosporioides decreases with rise in the concentration of plant extracts.

Potential use of Polyphenol-Enriched Extract from Moringa oleifera Leaves as an Active Ingredient in Sunscreen

Moringa oleifera is commonly referred to as the “tree of life” due to its high nutrient content and valuable biological activities. Widely found in tropical zones, this plant is usually used in local cuisine, cosmetic products and nutraceuticals. In this paper, we explored the potential of Moringa oleifera leaf alcoholic extract as a bioactive ingredient in sunscreen. Characterizations of extract were firstly investigated, including total polyphenol content, antioxidant activity. Formulations of extract containing creams were then created in order to examine its ability to protect against UVB radiation in the presence of other ingredients. Various evaluations of cream were carried out, including stability testing, UV absorption capacity, in vitro SPF values and microbial testing. The results revealed that Moringa oleifera extract showed a promising active ingredient in sunscreen, with a moderate antioxidant activity. Cream containing 2% of extract incorporated with 2% of oxybenzone demonstrated good in vitro SPF values.

The Evaluation of the Nutritional Effectiveness of Nano-Alcoholic Extract of Moringa oleifera Leaves added to Drinking Water on the Productive Traits of Broilers

This experiment was conducted in the poultry farm of the Department of Animal Production at the College of Agriculture / Al-Qasim Green University to evaluate the high nutritional effectiveness of the nano-alcoholic extract of Moringa oleifera leaves added to drinking water on the productive characteristics of broiler chickens. 180 one-day-old Ross broiler chicks, unsexed, were used. They were randomly divided into four treatments, 45 birds for each treatment, and each treatment consisted of three replicates (15 birds for each replicate). The experimental treatments were as follows: the first treatment (control) without adding the nano-alcoholic extract of Moringa oleifera leaves to the drinking water. The second treatment: adding the nano-alcoholic extract of Moringa oleifera leaves at a dose of 10 ml/liter of drinking water at a concentration of 0.01%. The third treatment: adding the extract Nano-alcoholic extract of Moringa oleifera leaves, at a dose of 10 ml/liter of drinking water, at a concentration of 0.02%. The fourth treatment: adding the nano-alcoholic extract of Moringa oleifera leaves, at a dose of 10 ml/liter of drinking water, at a concentration of 0.03%. The experiment included studying the following characteristics: average live body weight, weight gain, feed consumption, feed conversion factor, Percentage of losses, production index, Villus length, crypts depth, and the ratio of villus length to crypts depth. The results indicated that adding the nano-alcoholic extract of Moringa oleifera leaves led to a significant improvement in all the productive traits studied. It can be concluded from the current experiment that adding the nano-alcoholic extract of Moringa oleifera leaves to drinking water can lead to improving the productive traits of broiler chickens.

Wound-healing potentiation in mice treated with phenolic extracts of Moringa oleifera leaves planted at different climatic areas

For years, Moringa oleifera has been known for possessing wound-healing properties. This study aimed to investigate the effect of two extracts: aqueous extract (AE) and ethanolic extract (EE) of Moringa oleifera leaves planted at two regions (Mssisi and Lamta) in Morocco for their anti-inflammatory and healing properties, for which mice were used as a biological model. Inflammation was monitored by assessing forepaw volume of mice, measured at 0 min, 1 h, 3 h, and 5 h, after its induction by carrageenan. Hind paw of mice were treated with extracts of M. oleifera, at a dose of 50 mg/kg, obtained from Mssisi region. This resulted in reduction of edema by 99.2% with EE and by 91.8% with AE, compared to controls and the phenolic extract of M. oleifera planted at Lamta region. Regarding healing of burns induced on rat’s dorsal region; results showed that application of Moringa-based ointment for 14 days, at a dose of 50 mg/kg on wounds, resulted in total healing, compared to controls (negative control: more than 22 days, and positive control: 22 days). M. oleifera extracts resulted in nearly complete tissue repair of 98.26% and 95.34% with EE and AE, respectively.

Repellent and larvicidal properties of selected indigenous plants in the control of Anopheles mosquitoes

Background &amp; objectives: Widespread pyrethroid resistance and plastic-feeding behaviour of most malaria vectors across Africa threaten the efficacy of current insecticide-based vector control interventions like Insecticide-Treated Nets (ITNs) and Indoor Residual Spraying (IRS). This study examined the larvicidal activity ofMorinda citrifolia against Anopheles gambiae larvae and the repellent properties of Morinda citrifolia (Noni), Moringa oleifera (Moringa), and Ocimum basilicum (Basil) as complementary vector control tools against Anopheles gambiae sensu lato (s.l.). Methods: Noni, Basil, and Moringa oil extracts were obtained with the extraction techniques; Soxhlet, steam distillation and maceration respectively, using hexane and ethanol. The effectiveness of the extracts was assessed using the WHO standard larval susceptibility bioassay and guidelines for repellent efficacy. Following bioassays, effective doses (ED) and lethal concentrations (LC) were determined. Gas Chromatography-Mass Spectroscopy analysis was performed to identify the bioactive chemical components of the extracts of Moringa oleifera and Ocimum basilicum. Results: Emulsified Morinda citrifolia seed oil had LC50=68.3, LC90=130.9 and LC99.9=222.5, and ED99. 9=308.3%v/v, the ethanolic extract of Moringa oleifera leaves had ED99.9= 1.25g/ml, and essential oil of Ocimum basilicum leaves had ED99.9=0.28g/ml against Anopheles gambiae. Interpretation &amp; conclusion: The results obtained indicated that seed oil of Morinda citrifolia, essential oil of Ocimum basilicum, and crude extract of Moringa oleifera have repellent activity against An. gambiae s.l. The complete protection time (CPT) of Morinda citrifolia, Moringa oleifera, and Ocimum basilicum was 120 min, 72 min and 84 min at ED99.9 respectively. Morinda citrifolia oil exhibited larvicidal effects against the larvae of An. gambiae s.l. The results provide valuable information for the use of the plants as biocides.

Comparing efficacy of ethanol extract of Moringa oleifera L. leaves with some of antibiotic on the growth of bacteria Staphylococcus aureus

Comparing efficacy of ethanol extract of Moringa oleifera L. leaves with some of antibiotic on the growth of bacteria Staphylococcus aureus

Molecular diagnosis and phytochemical analysis of alcoholic extracts of Moringa oleifera leaves by GC-MS

Molecular diagnosis and phytochemical analysis of alcoholic extracts of Moringa oleifera leaves by GC-MS

In vivo toxicity study in Sprague-Dawley rats receiving different doses of Moringa oleifera extract

Background: Protein deficiency poses a significant challenge during the growth and development of children. Moringa oleifera Lam (MO) leaves, renowned for their high protein content, present a potential solution to address amino acid imbalances in protein-deficient conditions. Aim: This study aimed to evaluate the toxicity of MO leaves extract in Sprague-Dawley Rats. Methods: Protein extraction from Moringa oleifera (MO) leaves was performed using ultrasonic-assisted extraction (UAE) with ethanol. The ethanol leaf extract of MO (EEMO) was then characterized for protein content, amino acids, minerals, phytic acid, and phytochemicals. Antioxidant activity was assessed using the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) test, while cytotoxicity was evaluated using the MTT (3-(4,5-dimethylthiazolyl-2)-2,5 diphenyltetrazolium bromide) assay on HepG2 and MDCK cells. EEMO was tested for acute toxicity in 60 healthy male and female Sprague-Dawley rats. The rats were divided into five groups of six rats each, receiving single oral doses of 5, 50, 300, 2,000, and 5,000 mg/kg body weight (BW) of EEMO. Observations was conducted daily till day 14 before necropsy of rats. Liver and kidney tissues were harvested and preserved in 10% formalin for histopathological analysis. Results: The extraction process revealed a protein content of 45.5%, with phenylalanine being the predominant essential amino acid at 22.25 mg/g, and glutamic acid as the dominant non-essential amino acid at 60.03 mg/g. Potassium (1174.23 mg/100g) and selenium (149 mg/100g) were identified as the primary macro and micro minerals, respectively. The IC50 and CC50 values for antioxidant and cytotoxic activities in HepG2 and MDCK were found to be 41.04 ppm,182.66 ppm and 121.04 ppm respectively. Toxicity testing on experimental animals resulted in an LD50 value of 5,000 mg/kg BW for EEMO, indicating its relative safety upon oral administration. Conclusion: MO extract produced by the UAE extraction method, containing high-quality food-grade protein, showed no cytotoxic effects on HepG2 and MDCK cells and exhibited no acute toxicity in rats.

Phytochemical Assessment and Potential Pharmacological Activity of Moringa Oleifera Extract

Phytochemical Assessment and Potential Pharmacological Activity of Moringa Oleifera Extract