Beetroot Extract Research Articles

Bio-synthesized TiO2 nanoparticles and the aqueous binder-based anode derived thereof for lithium-ion cells.

Titanium dioxide nanoparticles (TiO2-NPs) are a promising anode material for Lithium-ion batteries (LIBs) due to their good rate capability, low cost, non-toxicity, excellent structural stability, extended cycle life, and low volumetric change (∼4%) during the Li+insertion/de-insertion process. In the present paper, anatase TiO2-NPs with an average particle size of ~ 12nm were synthesized via a green synthesis route using Beta vulgaris (Beetroot) extract, and the synthesized TiO2-NPs were evaluated as anode material in LIBs. Furthermore, we employed an aqueous binder (1:1 mixture of carboxy methyl cellulose and styrene butadiene) for electrode processing, making the process cost-effective and environmentally friendly. The results revealed that the Li/TiO2 half-cells delivered an initial discharge capacity of 209.7 mAh g-1 and exhibited superior rate capability (149 mAh g-1 at 20 C) and cycling performances. Even at the 5C rate, the material retained a capacity of 82.2% at the end of 100 cycles. The synthesis route of TiO2-NPs and the aqueous binder-based electrode processing described in the present work are facile, green, and low-cost and are thus practically beneficial for producing low-cost and high-performance anodes for advanced LIBs.

Antioxidant Activity Of Nanoemulsion Of Beet Fruit Extract Using The Dpph Method

Free radicals are one of many forms of reactive oxygen sorts known for having unpaired electrons. The presence of free radicals in the body can cause damage such as premature aging, characterized by the appearance of wrinkles. This damage can be countered with antioxidant compounds. Beetroot is one of the fruits with antioxidant properties due to the presence of betacyanin compounds from the flavonoid group. This research aims to identify the antioxidant activity and IC50 value of beetroot (Beta vulgaris L.) extract nanoemulsion formulations using the DPPH method. Nanoemulsion formulations were prepared with varying concentrations of beetroot extract at 1% (X1), 3% (X2), and 5% (X3) to determine which concentration has the best antioxidant activity. Quality evaluation of the nanoemulsion formulations was also conducted, including organoleptic tests, pH tests, and homogeneity tests. The results showed that the beetroot extract nanoemulsion formulations have antioxidant activity and meet the requirements for physical properties and formulation stability. Nanoemulsion formulations with 5% (X3) beetroot extract content exhibited the best antioxidant activity with an IC50 value of 136.475 ppm.

Encapsulation of beetroot extract (Beta vulgaris L.) obtained by internal and external ionic gelation: a comparative study

Natural pigments, like betalains found in beets, are sensitive to environmental conditions, which may impact their reactivity and shelf-life. Microencapsulation is an attractive alternative for delivering these compounds, offering protection through polymeric microcapsules. The aim of this study was to compare two ionic gelation methodologies, external (EG) and internal gelation (IG), in the microencapsulation of beet aqueous extract. Particles were obtained by mixing sodium alginate with the aqueous extract of beetroot and crosslinking with calcium chloride solution using the extrusion method. Encapsulation characteristics and physical, morphological features were evaluated. The particles showed 10.72 and 89.90% encapsulation efficiency for EG and IG, respectively. Loading capacity was 18.90% for EG and 25.60% for IG. Those IG particles showed superior water absorption capacity during rehydration. Texture analysis indicated that EG particles showed greater hardness. Release kinetics indicated that EG particles followed the Korsmeyer-Peppas model, while IG particles followed the Higuchi model. Thus, the appropriate encapsulation technique should be selected depending on the food matrix to be used and the specific objective of delivering the active encapsulated molecules.

Evaluation of the effect of Beetroot Dye on Banana Pseudo Stem and Pineapple Leaves Fibres

Extraction and characterization of dye from beetroot scientifically known as Beta vulgaris was achieved. The dye was extracted using two extraction method namely Soxhlet with methanol as solvent and water where the beetroot powder was soaked in water and heat at 30-400C for 2 hours. It was observed that due to instability of beetroot dye at high temperature, aqueous dye extraction is preferable over Soxhlet extraction as the one extracted with Soxhlet changed its color from purple to black brown. Moreso, the extracted dye was characterized. Qualitative photochemical analysis, Anti-bacterial activities, Fourier Transformer Infrared, Ultra Violet Spectroscopy are the analysis conducted. The photochemical analysis revealed the presence of Saponin, Flavonoids, Phenols, Betacyanin with the absence of Tanin and Glycosides. The anti-microbial activities of the dye was conducted using to microorganism namely, Escherichia coli and Staphylococcus aureus with Muller-Hinton Agar. The dye showed antimicrobial activities on Escherichia coli while it could not show any antimicrobial activities on Staphylococcus aureus microorganism. Also, UV-visible spectrum of the dye was conducted and the results showed the presence of betacyanins which are major compounds found in beetroot dye the major one being betanin. The presence of betanin in the dye was also confirmed by the FTIR as well as gas chromatography. Betanin is a major compound found in beetroot which is soluble in water due to the presence of polar groups such as COOH, OH, and NH2, thus the beetroot extract is a dye and not a pigment. Furthermore, dyeing of the extracted dye to treated banana pseudo stem and pineapple leaves fibres shows that it is feasible and the color fastness test conducted shows that both aluminum ammonium sulphate and aluminum acetate mordants can be used separately in simultaneous mordanting methods. Keywords: Beetroot, Extraction, Characterization, Dye, Banana pseudo-stem and pineapple leaves fibres and color fastness.

Natural Pigments-Based Two-Component White Light Emitting Systems.

In this paper, a new class of two component white light emitting systems viz, JaB (java plum + beetroot) {I}, and CaB (carrot + beetroot) {II} were developed through resonance energy transfer (RET) phenomenon by using a fruit (java plum) and two vegetable (carrot and beetroot) extracts. In these white light emitting systems, java plum and carrot are the donors while beetroot is the acceptor. The primary fluorescent pigments present in the natural extracts (i.e., anthocyanin in java plum, β-carotene in carrot, and betanin in beetroot) were responsible for the white light emission. The CIE (Commission Internationale d'Eclairage) coordinates for I and II were {0.32, 0.34} and {0.33, 0.33}, respectively, in solution phase. Interestingly, the white light emission (WLE) was also achieved in agar-agar gel medium. In gel medium, the CIE values were {0.31, 0.34} and {0.33, 0.32} for I and II, respectively. The donor-acceptor distance (r) for I and II were found to be 0.5 and 0.4nm, respectively. Furthermore, the rate of energy transfer was also quantified with the values of 2.78 × 109s-1 for JaB (I) and 1.02 × 108s-1 for CaB (II) systems. The mechanistic investigation of the two white light systems was further supported by DFT studies.

Introduksi Lipstik Cair dari Umbi Buah Bit (Beta vulgaris L.) di Pasantren Putri

A community service scheme for implementing science and technology has been carried out from Srwiijaya University funding sources. Community service is carried out at the Ittifaqiah Islamic Boarding School for women in Indralaya, Ogan Ilir, South Sumatra. Participants who attended were 60 Islamic boarding school students in the science class, 10 science teachers and 2 Ittifaqiah administrators. Community service was carried out using lecture methods and demonstrations of making liquid lipstick in front of female students and teachers. The natural ingredients used in making lipstick in this service activity are beet root extract, virgin coconat oil (VCO), soy lecithin, gum Arabic, food flavors and food preservatives. There were 20 organoleptic tests carried out on Unsri female students on the liquid lipstick made in this service activity. Fifteen people said they really liked it, 3 people said they liked it quite a lot and 2 people said they liked the product a little. The survey on the results of service activities showed that 50 female students said they really understood how to make liquid lipstick, 8 students said they understood quite well how to make liquid lipstick with the red color of beetroot and 2 female students said they understood a little.

Nephroprotective and Anti-Diabetic Potential of Beta vulgaris L. Root (Beetroot) Methanolic Extract in a Rat Model of Type 2 Diabetes Mellitus.

Background and Objectives: Diabetes mellitus is a chronic metabolic disease associated with several complications, including that of kidney disease. Plant-based dietary products have shown promise in mitigating these effects to improve kidney function and prevent tissue damage. This study assessed the possible favorable effects of beetroot extract (BE) in improving kidney function and preventing tissue damage in diabetic rats. Materials and Methods: Type 2 diabetes mellitus (T2DM) was induced using a low dose of streptozotocin (STZ). Both control and rats with pre-established T2DM were divided into six groups (each consisting of eight rats). All treatments were given by gavage and continued for 12 weeks. Fasting blood glucose levels, serum fasting insulin levels, Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), serum triglycerides, cholesterol, low-density lipoprotein-cholesterol, serum and urinary albumin, and creatinine and urea levels were measured. Apart from this, glutathione, malondialdehyde, superoxide dismutase, tumor necrosis factor-α, and interleukine-6 in the kidney homogenates of all groups of rats were measured, and the histopathological evaluation of the kidney was also performed. Results: It was observed that treatment with BE increased body weight significantly (p ≤ 0.05) to be similar to that of control groups. Fasting glucose, insulin, HOMA-IR levels, and lipid profile in the plasma of the pre-established T2DM rats groups decreased to p ≤ 0.05 in the BE-treated rats as the BE concentration increased. Treatment with BE also improved the renal levels of oxidative stress and inflammatory markers, urinary albumin, and serum creatinine and urea levels. Unlike all other groups, only the kidney tissues of the T2DM + BE (500 mg/kg) rats group showed normal kidney tissue structure, which appears to be similar to those found in the kidney tissues of the control rats groups. Conclusion: we found that streptozotocin administration disturbed markers of kidney dysfunction. However, Beta vulgaris L. root extract reversed these changes through antioxidant, anti-inflammatory, and antiapoptotic mechanisms.

Impact of a short-term nitrate and citrulline co-supplementation on sport performance in elite rowers: a randomized, double-blind, placebo-controlled crossover trial.

Citrulline (CIT) and beetroot extract (BR) have separately shown benefits in rowing performance-related outcomes. However, effects of combined supplementation remain to be elucidated. The main purpose of this research was to study the effects of 1week of daily co-supplementation of 3.5g BR (500mg NO3-) plus 6g CIT on aerobic performance, maximal strength, and high-intensity power and peak stroke in elite male rowers compared to a placebo and to a BR supplementation. 20 elite rowers participated in this randomized, double-blind, placebo-controlled crossover trial completing 1week of supplementation in each group of study: Placebo group (PLAG); BR group (BRG); and BR + CIT group (BR-CITG). 3 main physical tests were performed: aerobic performance, Wingate test and CMJ jump, and metabolic biomarkers and physiological outcomes were collected. The Wingate all-out test showed no between-condition differences in peak power, mean power, relative power, or fatigue index (P > 0.05), but clearance of lactate was better in BR-CITG (P < 0.05). In the performance test, peak power differed only between PLAG and BR-CITG (P = 0.036), while VO2peak and maximum heart rate remained similar. CMJ jumping test results showed no between-condition differences, and blood samples were consistent (P > 0.200). Supplementation with 3.5g of BR extract plus 6g of CIT for 7days improved lactate clearance after Wingate test and peak power in a performance test. No further improvements were found, suggesting longer period of supplementation might be needed to show greater benefits.

Effect of Beet Root (Beta vulgaris), White and Red Radish (Raphanus sativus and Raphanus longipinnatus) Juice Extract, on Salt Induced Hypertensive Wistar Rats

Background: Hypertension is one of the major global health challenges with high prevalence and undesirable complications, and is a major cause of premature death worldwide.&#x0D; Objective: This study was conducted to evaluate the effect of juice extracted from beetroot (BR), red radish (RR) and white radish (WR) tubers on experimentally induced hypertension in 10-week-old Wistar rats.&#x0D; Methodology: Total of 35 rats was randomly divided into seven (7) experimental groups A - G with 5 rats each. Rats in group A were not induced (normotensive group). All the rats in groups B-G were induced with hypertensive agent. Group B was not treated (negative control), groups C-G were treated simultaneously with a dose of 800 mg/kg body weight (orally) of BR, RR, WR, BR+RR combination, and standard drug (5mg Amlodipine) respectively. The study lasted for a period of 28 days during which the blood pressure and heart rate (HR) of each of the experimental rats were measured at 0, 7, 14, 21 and 28th day using Stethoscope technique. The interpretation of systolic blood pressure (SBP) and diastolic blood pressure (DBP) were made from pulse tracings using Aneroid Sphygmomanometer. The mean blood pressure (MBP) was calculated from SBP and DBP.&#x0D; Results: The BR, RR, WR and combination of BR+RR, did decrease the number of heart beats/minute from 192.00/minute to 208.80/minute which is within the normal base line, whereas the untreated rats recorded increase from 192.00/minute to 246.80/minute. The result showed that the hypertensive rats recorded higher (P&lt;0.05) mean values of SBP, the treated groups altered the rise from 110.40 mmHg to 100.40mmHg with the standard drugs, followed by the white radish treatment group, the combination of BR+RR, before RR, lastly the BR group. For DBP, at week 4, same impact reflected again, but week 2 and week 3 had WR to have the best effect, followed by BR +RR, then RR before the standard drug and then Beetroot. The BR + RR juice performed better than the individual RR and BR juice groups, suggesting possible synergistic effect.&#x0D; Conclusion: The 3 juice extracts had demonstrated varying degrees of protection, provided scientific evidence of their anti- hypertensive activities in an experimentally induced hypertension. Further studies are hereby recommended to isolate the specific biomolecule active agent(s) and investigate the mechanism of action.

Effect of beetroot extract on glucose-6-phosphate dehydrogenase, red blood cells, and their aggregation in a patient with Hereditary Spherocytosis: A case report

كثرة الكريات الحمر الوراثي هو مرض وراثي ينتج عنه تحلل كريات الدم الكروية وهذا يسبب فقر الدم الانحلالي وقد يكون مستخلص الشمندر ذات أهمية علاجية كبيرة بتأثيره على مرض كثرة الكريات الحمر وتكورها الوراثي. الهدف من الدراسة: تقييم اّثار استهلاك مستخلص الشمندر لمدة خمسة أسابيع على الشخص الحامل للمرض (المصاب) ومعرفة تأثيره على زيادة كريات الدم الحمراء وكثرة الكريات الحمر الوراثي (Hereditary Spherocytosis). المنهجية: تمت هذه الدراسة­ خلال شباط - نيسان ٢٠٢٣ في قسم الكيمياء في الجامعة المستنصرية وكان عدد المشتركين في البحث واحد فقط مصاب بتكور كريات الدم الحمراء ، اذ تم تشخيص محلول الشمندر بتقنية كروموتوغرافيا السائل عالي الأداء وبعدها أجراء تحليل الدم الكلي Complete Blood Count وقياس فعالية انزيم الكلوكوز -٦-فوسفات ديهيدروجينيز وتصوير كريات الدم الحمراء بالمجهر الالكتروني قبل وبعد تناول شراب الشمندر على مدى خمسة أسابيع كورس تجريبي للشخص المصاب.النتائج: أظهرت النتائج بنجاح تاثير مستخلص الشمندر على كريات الدم الحمراء لعينة المصاب ، اذ ظهرت زيادة بخلايا الدم والصفائح الدموية خلال الكورس التجريبي (خمسة أسابيع) ، كما لوحظ ارتفاع بنشاط الانزيم كلوكوز-٦-فوسفات ديهيدروجينيز داخل كريات الدم و داخل جزيئة الهيموغلوبين بعد مرور ٣ أسابيع. الأستنتاج: أن كورس مستخلص الشمندر الذي تم تطبيقه لخمسة أسابيع اعطى نتائج إيجابية في زيادة نسبة كريات الدم الحمراء بشكل ملحوظ لعينة المصاب مقارنة بالعينة السليمة، كما ان ارتفاع نشاط الانزيم G6PDH بعد تناول عصير الشمندر لمدة تتجاوز ثلاثة اسبيع يمكن ان يقلل من الاصابة بفقر الدم وبعض الامراض لما له من أهمية غذائية ومكونات رئيسية تساعد على تقوية المناعة وتعويض الدم المفقود.

Enhancing the accuracy, speed, and efficiency of kafirin-PEO electrospun bio-nanocomposite pH indicators with red beetroot extract using image processing.

Intelligent electrospun pH indicators were produced from bio-nanocomposite kafirin-polyethylene oxide (PEO) containing red beetroot extract. The aim was to evaluate the performance and stability of the electrospun pH indicators via image processing. Red beetroot extract was added to a mixture of kafirin and PEO at various concentrations. The mixtures were electrospun, and infrared Fourier transform spectroscopy confirmed the presence of kafirin, PEO, and red beetroot extract in the resulting pH indicator. The results showed that the pH indicators had high stability and reversibility at different temperatures, pHs, and environmental conditions. The results showed that the color of the indicators was significantly reversible after pH changes, with highly desirable reversibility observed at pH values of 1, 3, 4, 5, 7, 9, and 10. The findings proved that the red beetroot extract loaded bio-nanocomposite pH indicator accompanied by evaluation of color characteristics through image processing technique, can serve as a time-efficient, accurate tool for detecting and tracking pH changes caused by food spoilage.

THE INFLUENCE OF RED BEET VARIETIES AND CULTIVATION ON THEIR BIOCHEMICAL AND SENSORY PROFILE

Modern pharmacology shows that beetroot extracts exhibit antihypertensive and hypoglycaemic activity as well as excellent antioxidant activity. The promising results of the phytochemicals contained in the extracts suggest the opportunity for their use in functional foods.This study aimed to evaluate the biochemical profile (total ash and crude protein content, total polyphenols and betalains content) and sensory properties (5 points hedonic scale) of beetroot varieties from bio-B (B Taunus, B Boro, B Gesche) and traditional production-TP (TP Taunus, TP Boro, TP Rocket). The beetroots were obtained from a private producer from Austria. The crude protein content in evaluated red beetroots ranged from 1.54 % (B Taunus) to 5.32 % of fresh weight (TP Boro). The ash content range was 0.53 % (TP Rocket and TP Boro) to 0.63 % of fresh weight (TP Taunus). The total polyphenol content was the highest in sample of B Taunus – 3.29 mg GAE kg-1 of fresh weight (GAE – gallic acid equivalent). Betalains were determined separately as the red – violet betacyanins and the yellow betaxanthins. The concentration of betacyanins ranged from 5.7 mg.kg-1 (TP Boro) to 8.07 mg.kg-1 of fresh weight (B Gesche), while that of betaxanthins – from 2.77 mg.kg-1 (TP Boro) to 5.39 mg.kg-1 of fresh weight (B Gesche). Sensory properties (appearance, color, consistency, aroma, taste) of all the beetroot varieties were evaluated overall as good with the best score in a taste and overall appearance noted for TP Boro. The results improve our knowledge on beetroot as a valuable vegetable and indicate that further studies on the properties of different varieties of red beet are justified.

INVESTIGATING THE TOXICITY OF BETALAIN COMPOUNDS: IN SILICO ANALYSIS AND IN VIVO PREDICTIONS FOR STANDARDIZED BETA VULGARIS L. EXTRACT

Objective: Extensive research has been conducted on beetroot's antioxidant, hematoprotective, and cardioprotective properties. However, there currently needs to be more available evidence pertaining to the toxicity assessment of the extract. The toxicity assessment was conducted using both in silico and in vivo methods. Prior to testing, the extracts were standardized in accordance with the guidelines set by the Indonesian Food Drug Authority (BPOM), which is the regulatory authority for food and drugs in Indonesia. Methods: The experimental subjects consisted of 25 male Wistar rats in good health, weighing between 150 and 170 grams. These rats were separated into five groups, each including five rats. Group 1 will serve as the control group, while groups 2 through 5 will be designated as the treatment groups. The analysis of chemical toxicity was conducted using pK-CSM, SwissADME, and Pro-Tox II methodologies. Results: The results indicated that the standardized ethanol extract contained 4.341% water, 3.67 % total ash, and 1.53 % acid-insoluble ash. Lead (Pb) and cadmium (Cd) were absent at a concentration of 0 parts per million (ppm). Subsequently, the total plate count and yeast mould count were 0.47 5 x 10-4 (CFU/g) and a of 0.382 x 10-4 (CFU/g) respectively. This finding implies that the extract meets BPOM requirement. This study also measured the betalain content of red beetroot, yielding a total concentration of 11.34 0.37 mg/100 gram of sample. Haematological experiments showed that beetroot extract affected rat blood haematology. Compared to the control group, rats given the extract had higher red blood cell and platelet counts. Additionally, the Insilico toxicity test conducted on the active component derived from beetroot revealed LD50 of the compounds ranged from 305 mg/kg so that were categorized into classes IV and presence of hepatotoxic potential. During the in vivo experiment, there has been a notable rise in hepatic and renal parameters. Furthermore, one mortality event occurred in the test subject at a 5,000 mg/kg body weight dosage. Conclusion: Single oral administration of the extract at a dose larger than 5,000 mg per kilogram of body weight does not result in lethal effects, however showed potential toxicity to the liver.

Fermented Beetroot (Beta vulgaris L.) Extract by Kombucha as Energy Booster Produced via Cross-Flow Microfiltration Membrane

Beetroot extract (permeate) fermented by kombucha culture separated through cross-flow microfiltration (CFMF) with pore size 0.15 μm at TMP 2 and 6 bar for 0, 5, 15, 25, and 35 minutes, with a flow rate of 7.5 L/m3.hour, at room temperature potentially serves as a natural energy booster. The research results based on soluble protein show that the optimization process at TMP 2 and 6 bar was achieved at 15 minutes with permeate flux of 32.52 and 28.05 L/m3.h, respectively, and soluble protein content of 0.72 and 0.38 mg/mL, total solids of 3.40% and 3.36%, total sugar of 45.91 and 47.47 mg/mL, and acetic acid of 0.93 and 0.97%. Under these conditions, the CFMF system increased the soluble protein in permeate by 123.53% or 1.23 times, indicating partial rejection, and 22.58% or complete rejection. The identification of amino acids in the optimum condition was dominated by three threonine monomers with molecular weights of 120.11, 120.37, and 120.90 Da (M+), and six tryptophan monomers with molecular weights of 205.11, 205.21, 205.44, 205.60, 205.88, and 205.94 Da (M+). The permeate under optimum conditions had average particle sizes of 2788.0 nm and 2922.0 nm, with particle index of 1.523 and 1.795. The particle distribution in permeate at TMP 2 and 6 bar was less than 10,000 nm in size

Preparation and evaluation of natural lipstick from beetroot (Beta vulgaris) extract medicated with acyclovir

Preparation and evaluation of natural lipstick from beetroot (Beta vulgaris) extract medicated with acyclovir

Exploring beetroot (Beta vulgaris L.) for diabetes mellitus and Alzheimer's disease dual therapy: in vitro and computational studies.

This study explored the flavonoid-rich extract of beetroot (Beta vulgaris L.) for type 2 diabetes mellitus (T2D) and Alzheimer's disease (AD) dual therapy by using in vitro and molecular simulation studies. Flavonoid-rich extracts of B. vulgaris fruit were evaluated for their antidiabetic and anti-alzheimic activities. Molecular docking and dynamic simulation were performed to identify potential bioactive flavonoids with dual therapeutic effects on T2D and AD. Flavonoid-rich extracts of B. vulgaris fruit (IC50 = 73.062 ± 0.480 μg mL-1) had moderate activity against α-amylase compared to the standard acarbose (IC50 = 27.104 ± 0.270 μg mL-1). Compared with acarbose, flavonoid-rich extracts of B. vulgaris fruit had appreciable activity against α-glucosidase (IC50 = 17.389 ± 0.436 μg mL-1) (IC50 = 37.564 ± 0.620 μg mL-1). For AChE inhibition, flavonoid-rich extracts of B. vulgaris fruit exhibited (p < 0.0001) inhibitory activity (IC50 = 723.260 ± 5.466 μg mL-1), albeit weaker than that of the standard control, galantamine (IC50 = 27.950 ± 0.122 μg mL-1). Similarly, flavonoid-rich extracts of B. vulgaris fruit showed considerable (p < 0.0001) inhibitory effects on BChE (IC50 = 649.112 ± 0.683 μg mL-1). In contrast, galantamine (IC50 = 23.126 ± 0.683 μg mL-1) is more potent than the extracts of B. vulgaris fruit. Monoamine oxidase (MAO) activity increased in FeSO4-induced brain damage. In contrast, flavonoid-rich extracts of B. vulgaris fruit protected against Fe2+-mediated brain damage by suppressing MAO activity in a concentration-dependent manner. HPLC-DAD profiling of the extracts identified quercetrin, apigenin, rutin, myricetin, iso-quercetrin, p-coumaric acid, ferulic acid, caffeic acid, and gallic acid. Molecular docking studies revealed quercetrin, apigenin, rutin, iso-queretrin, and myricetin were the top docked bioactive flavonoids against the five top target proteins (α-amylase, α-glucosidase AchE, BchE, and MAO). Molecular dynamic simulations revealed that the complexes formed remained stable over the course of the simulation. Collectively, the findings support the prospect of flavonoid-rich extracts of B. vulgaris root functioning as a dual therapy for T2D and AD.

Microleakage Assessment of Hydrophilic Pit and Fissure Sealants Fortified with Green Synthesized Silver Nanoparticles-An in Vitro Study

Background and Aim: Pit and fissure sealants play a significant role in preventive dentistry. The sealant's physical properties lend a notable contribution to its long-term success. The invention of nanoscience has led to breakthrough discoveries in improving the mechanical properties of sealants. The current work aimed to evaluate the microleakage of Hydrophilic sealant fortified using silver nanoparticles compared to the Hydrophilic sealant and conventional sealant on permanent molars. Materials and methods: Silver nanoparticles were green synthesized using beetroot extract and incorporated into hydrophilic Ultraseal XT Hydro sealant. A sample of extracted third molars, thirty in number, were allocated randomly to three different groups as follows: Group I: conventional Clinpro sealant, Group II: hydrophilic Ultraseal XT hydro, and Group III: Ultraseal XT hydro fortified with silver nanoparticles. Microleakage assessment was performed using a stereomicroscope. The Kruskal-Wallis test was employed to compare the microleakage values of the three groups. Results: Group III had the lowest values about mean microleakage score , and the highest scores were noted for Group I ( . A statistically significant difference was observed in comparing the three groups employing the Kruskal-Wallis test. Conclusion: The results of the current study have established that the microleakage was the lowest in fortified UltraSeal XT Hydro (Group III) when compared to Ultraseal XT Hydro (Group II) and Clinpro sealant (Group I).

Enhancing beef sausage packaging with calcium alginate active film infused with nisin and ε-polylysine nanoparticles and beetroot extract

This study assessed the impact of calcium alginate (CA) film embedded with ε-polylysine nanoparticles (ε-PL-NPs) at concentrations of 500 and 1000 mg/L, or nisin nanoparticles (Ni-NPs) at 100 and 200 mg/L, both combined with beetroot extract (3 g/hg), on beef sausage quality. Compared to standard CA films, the active CA films with ε-PL-NPs demonstrated enhanced flexibility. The addition of Ni-NPs and ε-PL-NPs notably improved the CA films' barrier characteristics, with a reduction in water vapor permeability observed as nanoparticle concentration increased. CA films with ε-PL-NPs exhibited a more potent antimicrobial effect, effectively inhibiting a range of microbial growth, including total viable count, coliforms, Staphylococcus aureus, Clostridium perfringens, molds, and yeasts in beef sausages. Sausages packaged in CA films with ε-PL-NPs displayed reduced lipid oxidation and higher total phenolic content than those treated with Ni-NPs and the control samples. Consequently, CA films integrated with ε-PL-NPs at 1000 mg/L demonstrated promising capabilities in maintaining sausage quality, extending shelf life by 25%, and emerged as a novel packaging option for beef sausage with added beetroot extract. This innovative packaging approach also effectively decreased sausage nitrite levels, thereby addressing health concerns associated with nitrite consumption.

Comparative Efficacy of Red Beetroot Extract and Ozonated Olive Oil on Wound Healing in Rats

Comparative Efficacy of Red Beetroot Extract and Ozonated Olive Oil on Wound Healing in Rats

Smart packaging film prepared from subcritical water-modified oat starch and betalain of beetroot extract reinforced with cellulose nanofibrils

In this study, the smart food packaging films from subcritical water-modified native oat starch (NOS) were developed using betalain from beetroot as a pH biosensor was studied. NOS was modified by SCW at varying temperatures; henceforth modified oats starch (MOS) was designated as MOS@115, MOS@130, MOS@145, MOS@160, MOS@175, and MOS@190. FTIR, XRD, and gel permeation chromatography (GPC) results confirmed the crystallinity loss and molecular weight reduction of NOS. Improved cold water solubility and decreased viscosity were observed with increased temperature, which favored excellent film-forming features with improved elasticity. However, the modified starch's water-holding capacity and swelling rate increased until a certain temperature and afterward decreased. Improved physical and mechanical properties of the film were obtained after reinforcing with cellulose nanofibrils (CNF, 5 wt% of starch). Finally, MOS with betalain was efficiently demonstrated to monitor chicken spoilage. Thus, SCW treatment was investigated to be an efficient way to modify starch in an eco-friendly way for food packaging development.