Extraction Of Polysaccharides Research Articles

A highly efficient and environmentally friendly approach to utilizing Quercus suber pericarp subfractions to remove metallic cations from aqueous solutions

Increasing water contamination from metal cations is a significant environmental challenge for humans. This has sparked extensive research into the development of adsorbents with superior removal efficiencies. This study assessed the adsorption capacity of crude and lignocellulosic fractions obtained from the pericarp of Quercus suber acorns, specifically focusing on the removal of Pb(II), Cd(II), Ni(II), and Cu(II) ions from aqueous solutions. The lignocellulosic fraction was separated from the crude fraction after extraction of cell wall polysaccharides using water and alkaline solutions. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) were used to characterize the crude and lignocellulosic fractions. Data from equilibrium adsorption experiments were analyzed using the Langmuir and Freundlich isotherm models. The study found that the lignocellulosic fraction removed Pb(II) ions more efficiently than the crude fraction, with qmax values of 500.0 mg.g−1. Crude fractions exhibited a considerable retention potential for Cd(II), Ni(II), and Cu(II) ions with qmax values of 95.2 mg.g−1, 333.3 mg.g−1 and 200 mg.g−1 respectively. Crude and lignocellulosic fractions extracted from the acorn Q. suber pericarps could be environmentally friendly biosorbents for the removal of metal cations from aqueous solutions.

Extraction, Purification, Structural Characterization, and Antitumor Effects of Water-Soluble Intracellular Polysaccharide (IPSW-1) from Phellinus igniarius Mycelia.

Phellinus igniarius is a commonly used Chinese medicine fungus, and its polysaccharide is a valuable bioactive with antioxidant, antiaging, antitumor activities, etc. However, their bioactivities are influenced by their structural and physicochemical properties. Hence, this research isolated and purified homogeneous water-soluble intracellular polysaccharide (IPSW-1) from P. igniarius mycelia. A coherent study of its structural characteristics, conformation, and antitumor mechanisms was evaluated. The results showed IPSW-1 has no triple helical conformation according to the Congo red test. Based on FT-IR, periodate oxidation, Smith degradation, methylation analysis, 1H and 13C NMR spectroscopy data, and IPSW-1 consisted of α-d-glucopyranose (Glcp). The backbone of IPSW-1 consisted primarily of repeating three (1 → 6)-linked α-d-Glcp and one (1 → 3,4)-linked α-d-Glcp, with one terminal α-d-Glcp as side chains of 3-O-connected to the main chain for every four residues. The IPSW-1 had an inhibitory influence on HepG2 cell proliferation and inhibited the migration and invasion ability by down-regulating the expression levels of MMP-7 and RhoA. Moreover, IPSW-1 could inhibit the lysis of autophagosomes to inhibit autophagy and regulate mitochondrial membrane potential and pro-apoptotic protein Bax, which causes the caspase cascade to promote apoptosis, thereby inhibiting the role of tumor cells. These findings show IPSW-1 holds potential as an innovative functional food.

Evaluation of fibrous feed ingredients alternatives to oat hulls as a source of feed structure in broiler diets

This study aimed to evaluate several fibrous feed ingredients as potential substitutes for oat hulls (OH), assessing their efficacy in providing structural integrity to broiler feeds. A total of 4,160 day-old male Ross-308 broilers were allocated to eight dietary treatments, including a control group (CON) without additional fiber supplementation and 7 diets where 3% of the wheat content was replaced by either OH, soy hulls (SH), beet pulp (BP), carob bean (CB), wheat straw (WS), rice hulls (RH), or wheat bran (WB). The experimental design followed a complete randomized block design with 10 pens per treatment and 52 birds each. Growth performance and gut development indices were monitored, and the coefficients of total tract apparent retention (CTTAR) of nutrients were measured at 28 d. The OH improved feed conversion ratio (FCR) during the entire growth period (1-36 d) compared to the CON, SH, CB, WS, RH, and WB (P < 0.05). Conversely, BP diets reduced the final BW and ADFI compared to OH (P < 0.05) but were not different from the CON (P > 0.05). However, the FCR in birds fed with BP was similar to OH but lower than the CON group. In addition, BP-fed birds had higher CTTAR of ether extract and non-starch polysaccharides and relative weight of empty proventriculus and gizzard to BW at 14 and 28 d compared to CON. The WS, RH, and WB yielded similar final BW to OH and CON but higher FCR (P < 0.05). The CB, on the other hand, resulted in the highest FCR when contrasted with the other substitutes and CON (P < 0.05). Finding an alternative to OH with comparable benefits remains a challenge, with WS, RH, and WB showing similar final BW but inferior FCR to OH, and BP showing similar FCR but lower BW and ADFI.

Review: research progress on the extraction and utilization of polysaccharide components in grey dates

Abstract China is the largest producer of jujubes in the world, with the Xinjiang region accounting for about 50 % of the total production. However, local jujube processing still relies on traditional methods, leading to reduced industrial efficiency. Research shows that jujube polysaccharides possess various biological properties, such as antioxidant, anti-tumor, and anti-inflammatory activities. Enhancing the extraction of jujube polysaccharides to boost their food, medicinal, and economic value has become an important development direction of the jujube industry. This paper reviews the current research on the active functions of grey jujube polysaccharides, extraction technologies, and recent advancements in their production and application. It systematically introduces both traditional and emerging extraction technologies and summarizes the current state of production and application of grey jujube polysaccharides. The aim is to provide references for other researchers interested in jujube polysaccharides and to extend the value chain of the grey jujube industry.

Ultrasound-assisted extraction of polysaccharides from Ginkgo biloba: Process optimization, composition and anti-inflammatory activity

Plant derived polysaccharides can enhance immune function in the human body, effectively prevent diseases, and reduce the probability of bacterial infections. Ginkgo crude polysaccharide (GCP) was obtained from Ginkgo biloba by ultrasonic-assisted hot water extraction. Our data showed that the best extraction conditions of GCP were as follows: extraction temperature 80 °C, ultrasonic time 35 min, extraction time 3 h, and solid‒liquid ratio 1:30. Fourier transform infrared spectrometer (FT-IR) data showed that this polysaccharide might be an acidic polysaccharide with a carboxylic acid ring structure. Further studies implied that GCP was mainly composed of glucose, galacturonic acid, rhamnose, galactose and arabinose, accounting for 39.45 %, 25.01 %, 15.40 %, 11.94 % and 4.25 %, respectively. 0.1, 1 and 10 mg/mL GCP reduced the release of inflammatory factors in RAW264.7 cells via inhibition of the nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) signalling pathway. GCP was separated into five components with different molecular weights by an ultrafiltration membrane. Our data showed that GPa with a molecular weight ≥100 kDa was the main component of GCP. 1 mg/mL GPa, GPb, GPc and GPd had anti-inflammatory activities, and 1 mg/mL GPa had the best anti-inflammatory activities. Our results preliminarily reveal the elements and biological activity of GCP, which will provide a reference for the development of Ginkgo biloba.

Comparative Chemical Analysis and Bioactive Properties of Aqueous and Glucan-Rich Extracts of Three Widely Appreciated Mushrooms: Agaricus bisporus (J.E.Lange) Imbach, Laetiporus sulphureus (Bull.) Murill and Agrocybe aegerita (V. Brig.) Vizzini.

Herein we describe the antioxidant, antimicrobial, antibiofilm, anti-inflammatory and wound-healing potential of aqueous and polysaccharide extracts from three widely appreciated mushrooms: Agrocybe aegerita, Laetiporus sulphureus and Agaricus bisporus. Moreover, we present their detailed phenolic, polysaccharide and protein profiles and ATR-FTIR spectra. The study found that polysaccharide extracts (PEs) from mushrooms had higher total and β-glucan levels than aqueous extracts (AEs), with A. aegerita showing the highest content. L. sulphureus had a higher total protein content, and A. aegerita AE had the highest phenolic content. Our results indicate that all the tested extracts have high potential regarding their bioactive properties, with A. aegerita being the most promising one. Namely, the antibacterial activity assay showed that the development of the skin-infection-causing agent, Staphylococcus aureus, was inhibited with a minimal inhibitory concentration of 4.00 mg/mL and minimal bactericidal concentration of 8.00 mg/mL, while the results regarding wound healing showed that, over the course of 24 h, the A. aegerita extract actively promoted wound closure in the HaCaT keratinocyte cell line model. The anti-inflammatory activity results clearly showed that when we used S. aureus as an inflammation-inducing agent and the A. aegerita aqueous extract in treatment, IL-6 levels reduced to the level of 4.56 pg/mL. The obtained data suggest that the tested mushroom extracts may serve as a source of bioactive compounds, with potential applications in the cosmeceutical, pharmaceutical and food industries. Furthermore, potential skin preparations carefully crafted with mushroom extract may help restore the skin's barrier function, decrease the probability of staph infections and minimize skin irritation.

Utilization of electron beam irradiation pretreatment for the extraction of pectic polysaccharides from Diaphragma juglandis fructus: Structural, physicochemical, and functional properties

The effects of electron beam irradiation (EBI) pretreatment on the alkaline extraction of pectic polysaccharides from Diaphragma juglandis fructus (DJF) are highly dependent on the irradiation dosage. Comprehensive characterizations encompassing physicochemical, structural, and functional properties were conducted on crude pectic polysaccharide extract from DJF subjected to various EBI doses. EBI pretreatment significantly increased the yields of crude pectic polysaccharides extract (increasing by 41.89 %), also facilitating the extraction of uronic acid, RG-I structure, and protein content, despite causing a decrease in total sugar content. EBI pretreatment induced the degradation of pectin, resulting in decreased molecular weight, particle size, crystallinity, viscosity, thermal stability, and water holding capacity, while enhancing solubility and oil holding capacity. Variations in physicochemical and structural properties induced by different EBI doses influenced the functional activities of DJF pectic polysaccharides. Low-dose EBI (at 5 kGy) pretreatment markedly improved the emulsifying activity/stability (increasing by 20.82/74.10 %) and ABTS/DPPH radical scavenging activity (increasing by 27.91/12.40 %), whereas high-dose EBI pretreatment (50 kGy) greatly enhanced foaming capacity/stability (increasing by 259.99/175.56 %). These findings provide a novel regulatory strategy for the functional activity of pectic polysaccharides.

Extraction, purification, characterization and antidiabetic mechanisms of plant polysaccharides: a critical review

Abstract Diabetes mellitus (DM) represents a heterogeneous and multifactorial metabolic disorder, leading to diverse economic challenges and health complications, significantly affecting individuals’ lives. Type 2 diabetes (T2DM) stands as the predominant form of diabetes, with its onset influenced by a range of genetic and environmental factors. Currently, conventional diabetic medications are employed in clinical practice, but they may entail certain side effects. Fortunately, diverse natural polysaccharides extracted from plants exhibit antidiabetic properties, which have been demonstrated to effectively mitigate hyperglycemia, reverse insulin resistance, and forestall complications associated with diabetes. Consequently, exploring the utilization of these polysaccharides as adjuncts to conventional therapies has emerged as a novel research direction in recent years. Thus, this review centers on the extraction, purification, and characterization of plant polysaccharides, providing an overview of the advancements in the antidiabetic effects and mechanisms of natural polysaccharides. This perspective offers fresh insights to explore potential research domains and enhances understanding for the development of diabetic drugs derived from polysaccharides.

A salt/salt aqueous two-phase system based on pH-switchable deep eutectic solvent for the extraction and separation of mulberry polysaccharides

With the aim of expanding the potential application scope of mulberries, eleven pH-switchable deep eutectic solvents were screened for the ultrasonic-assisted extraction of mulberry polysaccharides, and a salt/salt aqueous two-phase system was constructed for the efficient separation of mulberry polysaccharides by regulating the system pH. DES-9 (tetraethylammonium chloride: octanoic acid molar ratio = 1: 2) with a critical response pH value of approximately 6.1 was concluded to be the best extraction solvent for extracting mulberry polysaccharides. A maximum polysaccharide extraction yield of 270.71 mg/g was obtained under the optimal conditions. The maximum polysaccharide extraction efficiency was 78.09 % for the pH-driven tetraethylammonium chloride/K2HPO4 aqueous two-phase system. An acidic β-pyran mulberry polysaccharide with a low-molecular weight of 9.26 kDa and a confirmed monosaccharide composition were obtained. This efficient and environmentally friendly polysaccharide separation method offers a new approach for the efficient extraction and utilization of other plant polysaccharides.

Extraction, characterization, and neuroprotective effects of polysaccharides the stems of Panax ginseng C. A. Meyer by three different solvents (water, acid, alkali)

The purpose of this study was to optimize the extraction process of polysaccharides from the stems of Panax ginseng C. A. Meyer using different solvents (water, acid and alkali), and investigate their protective effects on PC12 cells against Amyloid-β1–42 (Aβ1–42)-induced cell apoptosis. Three polysaccharides (W-GSP, Ac-GSP and Al-GSP) were prepared and optimized: liquid to material ratio 27:1 mL/g, extraction temperature 89°C, extraction time 2.9 h and 0.3 mol/L HCl/NaOH. The results of characterization indicated that the monosaccharides of W-GSP (703.80 kDa), Ac-GSP (641.38 kDa) and Al-GSP (457.04 kDa) all composed mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose with different molar ratios. Interestingly, W-GSP possessed a lower uronic acid, higher molecular weight, rougher surface and poorer thermal stability, whereby W-GSP exhibited a protective effect against Aβ1–42-induced apoptosis in PC12 cells by mediating the JNK signaling pathway, restoring the mitochondrial membrane potential and reducing intracellular ROS levels. This study should be provided the theoretical basis for the study and exploitation of polysaccharides from the stems of Panax ginseng C. A. Meyer.

Simultaneous extraction of oil, protein and polysaccharide from Idesia polycarpa Maxim cake meal using ultrasound combined with three phase partitioning

This study explored the potential of ultrasonic-assisted three-phase partitioning (UTPP) to simultaneously extract lipids, proteins, and polysaccharides from Idesia polycarpa Maxim (IPM) cake meal, a significant byproduct of oil extraction. The impact of variables such as inorganic salt type, solid–liquid ratio, salt concentration, pH, ultrasonic time, temperature, and volume of dimethyl carbonate was examined. Based on the single-factor tests and response surface methodology (RSM), optimal conditions were identified as 30 % ammonium citrate, a 1:26 solid–liquid ratio, pH 3, 31 min of ultrasonic time, 30 °C temperature, and 15 mL of dimethyl carbonate. These conditions achieved extraction rates of 8.10 % for lipids, 5.03 % for proteins, and 10.03 % for polysaccharides, with recovery rates of 91.62 %, 83.08 %, and 93.95 % respectively. Chemical analysis showed the lipid fraction rich in linoleic acid, and the protein fraction high in glutamic acid, aspartate, and serine. The polysaccharide fraction, mainly RG-I pectin with a molecular weight of 226.58 kDa, exhibited strong thermal stability and inhibitory effects on α-glucosidase and glycation, suggesting potential for functional food and dietary supplement applications. This highlights UTPP as a sustainable method for effectively utilizing valuable compounds from IPM cake meal, outperforming traditional extraction techniques.

The ultrasonic-assisted enzymatic extraction, characteristics and antioxidant activities of lychee nuclear polysaccharide

A response surface methodology (RSM) and one-factor method were established to investigate the optimum conditions for the extraction of lychee nuclear polysaccharides (LSP) by ultrasonic-assisted pectinase. The content of basic components in polysaccharides was determined. The antioxidant activity was well determined and compared the differences in the activities of the polysaccharides extracted by water extraction (LSP-HW) and those extracted at ultrasound-assisted enzyme (LSP-UAE). The activity of lychee nuclear polysaccharide increased with the increase of concentration. The anti-hydroxyl radical and anti-lipid peroxidation abilities of lychee nuclear polysaccharide were more excellent, and LSP-HW was slightly better than LSP-UAE in terms of activity, but the difference was not significant. In terms of solubility; LSP-HW without deproteinization was the best, followed by LSP-UAE, and the worst was LSP-UAE after deproteinization. The higher the glycoprotein content, the better the solubility of its polysaccharide. Compared with the existing extraction methods, this experiment greatly improved the extraction rate of lychee nuclear polysaccharides and further enriched the antioxidant activities of polysaccharides.

Punica granatum L. polysaccharides: A review on extraction, structural characteristics and bioactivities

Punica granatum L., commonly known as pomegranate, is native to Afghanistan and Iran, and today widely cultivated all over the world. Pomegranate polysaccharides are one of the most important bioactive components of P. granatum, which have a wide range of beneficial biological activities, such as anticancer, immunostimulatory, hepatoprotection, anti-psoriasis and antioxidation. Hot water extraction is currently the most commonly used method to isolate pomegranate polysaccharides. The structural characteristics of pomegranate polysaccharides have been extensively investigated through various advanced modern analytical techniques. This review focuses on the extraction, purification, structural characteristics, biological activities and structure-activity relationships of polysaccharides from Punica granatum. The aim of this article is to comprehensively and systematically summarize recent information of polysaccharides from Punica granatum and to serve as a basis for further research and development as therapeutic agents and functional foods.

Polysaccharides from Hericium erinaceus and its immunomodulatory effects on RAW 264.7 macrophages

This study aimed to optimize the extraction of Hericium erinaceus polysaccharides (HEP) using ultrasound-assisted enzymatic extraction combined with Plackett-Burman design (PBD) and response surface methodology (RSM). The optimal extraction conditions were identified as: 33 min extraction time, 30:1 liquid to material ratio, 38 °C extraction temperature, 9 g/kg cellulase amount, pH 4, and 20 % ethanol concentration. Under these conditions, the extraction yield of HEP was 5.87 ± 0.16 %, consistent with the predicted results. Additionally, the potential immunomodulatory activity of HEP on RAW 264.7 macrophage was evaluated. The results revealed that HEP improved the immunostimulatory activity of RAW264.7 cells, evident from increased production of IL-6 and TNF-α. These findings suggest that HEP is capable of enhancing the immune activity of RAW 264.7 macrophage.

Interaction between soy hull polysaccharide and mucin with sodium-/potassium-ion treatment: interfacial property

Abstract The interaction between soy hull polysaccharide and intestinal mucus is influenced by different ions. This study investigates the effects of various concentrations of Na+/K+ on the stability, interfacial activity, and viscoelasticity of the microwave-assisted ammonium oxalate extraction of soy hull polysaccharide (MASP) in simulated intestinal fluids (SIFs) in terms of particle size, zeta potential, surface hydrophobicity, Fourier-transform infrared spectroscopy, shear rheology, and microstructure. Results showed that 1.5 % Na+/K+ causes MASP and mucin in SIFs to bind through physical adsorption; the average particle size of the MASP/SIF miscible system increases from 1,659 to 1,881 and 1,736 nm. When the ion strength is 0.5 %, MASP rapidly reduces the SIF interfacial tension. The addition of Na+/K+ causes a slight redshift in the protein amide I band and an increase in the α-helix content with respect to the decrease in the β-turn and β-sheet corner content. Furthermore, 0.5 % content of Na+ and K+ causes small MASP particles to adsorb onto the surface of mucin, while 1.5 % content of Na+ and K+ causes MASP particles to arrange above mucin after 12 h. These findings provide reference for a deeper understanding of the selection of macroelements (sodium and potassium) in the intake of polysaccharide.

Clinacanthus nutans crude polysaccharide extract as a green platform for microwave-assisted synthesis of silver nanoparticles: Optimization, characterization, and evaluation of bioactivities

Clinacanthus nutans (C. nutans) is a plant in tropical Asia with proven biological activities. The optimized extraction method of C. nutans crude polysaccharide (CNP) uses water in the presence of an ultrasound-assisted mechanical method (UL_CNP). However, the use of UL_CNP for the synthesis and optimization of silver nanoparticles (AgNP), particularly their anticancer and photocatalytic properties, remains unexplored. Hence, this research aimed to employ a green method using UL_CNP and silver nitrate to produce AgNP (UL_AgNP) with a small size and assess its potential toxicity, anticancer, and photocatalytic activities. The synthesis condition was optimized using the Box–Behnken design method. The synthesized UL_AgNP showed the surface plasmon resonance peak at 458 nm. The optimized synthesis condition produced spherically shaped UL_AgNP with a size of 5.21 ± 1.92 nm and a zeta potential of −26.33 ± 0.93 mV. An X-ray diffraction analysis exhibited intense Bragg's reflection peaks at (111), (200), (220), and (311), having a face-centered cubic structure of AgNP. Attenuated total reflectance-Fourier-transform infrared spectroscopy and energy-dispersive X-ray spectroscopy further confirmed the presence of silver in the synthesized UL_AgNP. The brine shrimp lethality test of UL_AgNP reported a lethal concentration 50 value of <7.8 μg/mL after 24 h. The UL_AgNP exhibited antiproliferative activity against MCF-7 cells with a half-maximal inhibitory concentration value of 4.96 ± 0.31 μg/mL by inducing S-phase cell cycle arrest, apoptotic effect, and reduction of cell migration. Furthermore, UL_AgNP proved its efficient photocatalytic activity against methylene blue dye (50.22 % ± 0.06 %, after 10 min at a concentration of 50 μg/mL). Therefore, the UL_AgNP exhibited promising antiproliferative activity against MCF-7 cells, highlighting their potential as a therapeutic agent. Further investigations are needed to elucidate the precise mechanism of their action.

Optimization of sequential ultrasound-microwave assisted extraction of polysaccharide from red seaweed (Kappaphycus alvarezii)

Optimization of sequential ultrasound-microwave assisted extraction of polysaccharide from red seaweed (Kappaphycus alvarezii)

Pasting, rheology, structural properties and in vitro digestibility of potato starch complexes co-gelatinized with squash polysaccharides

The effects of extraction of squash polysaccharide from water (WESP) and subcritical water (SWESP) on gelatinization, rheology, thermodynamic, micro-structure, freeze-thaw stability and digestibility of potato starch (PS) were studied. The WESP and SWESP decreased peak viscosity, trough viscosity, breakdown viscosity, final viscosity, storage modulus and loss modulus of PS. Conversely, the setback viscosity, pasting temperature and peak time increased. All gel samples belonged to the weak gel system. The thermodynamic and fourier spectroscopic results demonstrated a significant retardation of PS retrogradation in the presence of WESP and SWESP. It was consistent with the microstructure characterization detected by scanning electron microscopy that both WESP and SWESP had a significant inhibitory effect on the formation of the PS gel structure. Moreover, the existence of WESP and SWESP compromised the freeze-thaw stability of PS gel. Meanwhile, WESP and SWESP also affected the digestibility of PS and reduced the eGI value of PS, which was conducive to stabilizing blood sugar fluctuations. This study helped to better understand the interaction of PS with WESP/SWESP in the gel system, and supplied a way to improve the quality of PS-related products. In addition, it also contributed to the industrial production and application of WESP and SWESP.

Effect of extraction methods of polysaccharides from Tricholoma mongolicum Imai on digestion and fecal fermentation in vitro

This study employed extraction methods, namely acid, alkaline, ultrasonic-assisted, hot-water, and dual enzyme-assisted extraction to extract polysaccharides from Tricholoma mongolicum Imai (TMIPs), and investigated them for intestinal digestion and fecal fermentation in vitro. Furthermore, using fructo-oligosaccharide as a positive prebiotic control, the impact of these TMIPs as carbon sources on the growth of Lactobacillus and Bifidobacterium in liquid culture was assessed. The results showed that all fractions transit through the gastrointestinal tract without degradation. Additionally, compared to the control group, the five polysaccharides significantly promoted the growth of probiotics, with a significant increase in short-chain fatty acid production after 48 h of fermentation. Furthermore, all five polysaccharides modulated the composition of gut microbiota. This offers theoretical guidance in the rational advancement of functional products derived from edible mushrooms, aiming to enhance gastrointestinal health in humans.

Extraction of water-soluble polysaccharides from lupin beans and their function of protein dispersion and stabilization under acidic conditions

In the current environment whereby new sources of proteins are extracted from plant material, it is also important to study the potential use of the resulting side streams. Although a number of studies have been conducted on various polysaccharides extracted from plant raw material, a polysaccharide fraction extracted from lupin bean is yet to be explored, in spite of the emerging interest in this crop as a source of food ingredients. In this work lupin soluble polysaccharide (LuPS) was obtained with a recovery as high as 46 % by extraction at pH 8, 120 °C, for 90 min. This fraction, named LuPS-8, was composed of a mostly linear pectic polysaccharide with a weight average molecular mass of 6608 kg/mol, and containing 71.0 % galactose, with minor amounts of arabinose (16.0 %), glucuronic acid 4.6 %, and galacturonic acid 4.1 %. When added to an acid milk dispersion, LuPS-8 improved its dispersibility, providing storage stability against sedimentation over a wider pH range than a HM-pectin reference, between 3.6–4.4. This research demonstrated the potential for upcycling of a side stream of lupin protein production, by the creation of value-added novel functional polysaccharide.