Milk Extract Research Articles

Rebirth of recycling liquid chromatography with modern chromatographic columns : Extension to gradient elution

Twin column recycling semi-preparative liquid chromatography (TCRLC) is revived to prepare small amount (∼ 1 mg) of a pure targeted compound, which cannot be isolated by conventional preparative liquid chromatography. In this work, TCRLC is extended to gradient elution. The first step of this modified process consists of a gradient step, which eliminates both early and late impurities. If not discarded, some late impurities could echo during the second isocratic recycling step of the process and compromise the purity level required for the targeted compound. Additionally, the entire gradient TCRLC (GTCRLC) process is automated regarding the eluent composition programmed and the actuation times of two valves: one two-position four-port divert valve enables to shave the targeted compound from early and late impurities during the initial gradient step. The second two-position six-port recycling valve ensures the complete baseline resolution between the band of the targeted compound and those of the closest impurities, which are not fully eliminated after the initial gradient step. The automation of the whole GTCRLC process is achieved by running four preliminary scouting gradient runs (at four different relative gradient times, tgt0= 2, 6, 18, and 54, where t0 is the hold-up column time) for the accurate determination of the thermodynamics (lnk versus φ plots of the retention factor as a function of the mobile phase composition) of the first impurity, the targeted compound(s), and of the last impurity. The automated GTCRLC process was successfully applied for the isolation of a polycyclic aromatic hydrocarbon (PAH), chrysene, from a complex mixture of PAHs containing two nearly co-eluting impurities (benzo[a]anthracene and triphenylene) and nine other early/late impurities (sample volume injected: 1 mL, 7.8 mm × 150 mm Sunfire-C18 column, acetonitrile/water eluent mixtures, T= 55 ∘C, 20 cycles, baseline separation in less than two hours). Additionally, the GTCRLC process is advantageously used to isolate and baseline separate the vitamins D2 and D3 initially present in a milk extract mixture (0.3 mL sample injection volume, 7.8 mm × 150 mm Sunfire-C18 column, methanol/water eluent mixtures, T= 65 ∘C, 14 cycles needed in 1.5 hours). These results open promising avenues toward an effective preparation of unknown targeted compounds before further physico-chemical characterization and unambiguous identification.

Influence of enzymes and extraction conditions on high yield of cottonseed milk

Aim: This research aimed to optimize suitable hydrolytic enzymes for maximizing cottonseed milk extracts for high cottonseed milk yield, protein content and low gossypol level. Methodology: Known amount of cottonseed was soaked for 90 min at 32°C and blended (cottonseed:water@1:6). Different aliquots of the blended cottonseed slurry were treated with 1% of enzymes viz., protease, cellulase and α-amylase enzyme at pH 7.0 followed by incubation at 40 and 52°C for 2.30 hr for the extraction of cottonseed milk. The enzyme activity of extracted milk was subsequently inactivated by pasteurization (90°C, 5 min). Further analysis of physico-chemical characteristics was also carried. The control sample included milk extraction from non-enzyme treated cottonseed milk extract (30±2°C). Results: Among different treatments, cottonseed milk extraction using protease enzyme at 40°C incubation showed the highest milk yield (86.71%) with the lowest sedimentation (3.72%). Further incubation 40°C and 52°C showed the highest protein content of 2.10 and 2.27 g 100 ml-1 and gossypol reduction of 40.36 and 35.22%, respectively, in the cottonseed milk extract. Meanwhile, cellulase and α-amylase enzymes treated samples at both incubation temperatures showed poor physico-chemical characteristics as compared to control. Interpretation: Protease enzyme seems to be the most suitable for optimum or higher extraction of cottonseed milk.

Single and combined effects of regulated and emerging mycotoxins on viability and mitochondrial function of SH-SY5Y cells

Co-occurrence of emerging and regulated mycotoxins in contaminated samples has been widely documented, but studies about their combined toxicity are scarce. In this report, the regulated mycotoxins deoxynivalenol, fumonisin B1 and zearalenone, and the emerging ones enniatin A, enniatin B and beauvericin were tested in SH-SY5Y human neuroblastoma cells. Their individual and binary combined effects on cell viability and mitochondrial function were evaluated. The results with individual mycotoxins revealed that deoxynivalenol and emerging mycotoxins were the most damaging to neuronal cells, presenting IC50 values between 0.35 and 2.4 μM. Interestingly, non-regulated mycotoxins triggered apoptosis by affecting to mitochondrial membrane potential. However, when regulated and non-regulated mycotoxins were binary mixed, antagonistic effects were found in all cases. Finally, cow feed and milk extracts were analysed by UHPLC-MS/MS, detecting the presence of several mycotoxins included in this study. These extracts were tested in neuroblastoma cells, and damaging effects on cell viability were found. Although binary combinations of mycotoxins produced antagonistic effects, their mixture in natural matrixes induces greater effects than expected. Therefore, it would be interesting to explore the matrix influence on mycotoxin toxicity, and to continue studying the neurotoxic mechanism of action of emerging mycotoxins, as they could be a health hazard.

Determination of Potassium Levels in Bananas Using an Optical Sensor with a Flat and Concave Mirror Plane

Potassium is a nutrient that plays a role in maintaining the function of the muscles and nerves that control the heart and is needed for body stability. The potassium content in the body can be obtained from foods such as bananas. The development of instruments and methods that are developed to obtain a more accurate measurement of potassium concentration requires an instrument that has high linearity and sensitivity. The instrument is in the form of an optical sensor system equipped with the use of optical fibers to guide the waveform to maintain its intensity stability. In this study, an experimental method was conducted with a sample of a standard solution with potassium as the solute and pure water as the solvent. Then continue to measurements on samples of banana milk and green banana fruit extracts. The results of the analysis of the measurement data using an optical sensor with a concave mirror reflection plane obtained a sensitivity of 0.36 mV/ppm and a linearity of 82.56%. In the plane of the flat mirror reflection, obtained an optical sensor with a plane mirror reflection plane shows a sensitivity of 0.12 mV/ppm and a linearity of 97.6%. The highest and most accurate linearity value is found in the plane mirror plane results. The next stage is the result of the maximum output voltage read on the optical detector through an optical sensor with a sample of extracts of milk banana and green banana. The results of data analysis on the linear equation with the highest linearity obtained the potassium content in milk bananas of 391.54 ppm and the green banana extract solution obtained 307.91 ppm, so it can be concluded that the potassium content in milk bananas is higher than green bananas with a linearity of more than 97%.

Green emitting SrAl2O4:Tb3+ nano-powders for forensic, anti-counterfeiting and optoelectronic devices

Tb3+ ions incorporated strontium aluminate nanopowders were prepared via eco-friendly green ultrasonication followed by combustion method by using Carica papaya milk extract as a fuel/surfactant. The prepared powders were characterized by advanced characterization techniques. X-ray diffraction profiles of the prepared samples confirmed the monoclinic phase of SrAl2O4:Tb3+ (1–11 mol%) and well consistent with PDF number of 74-0794. Rod, mesh and spikes like morphology were confirmed through SEM images. TEM images showed the irregular extended particles with smooth surface and confirm the polycrystalline nature of prepared nanopowders. Upon excitation at 374 nm the photoluminescence emission spectra show intense peaks in the range at 480–620 nm due to the transitions 5D4 → 7FJ (J = 6, 5, 4 and 3) of Tb3+ ions. CIE, CCT and CP results observed were corresponding to intense green colour with (0.21, 0.71), 4621 K and 90% respectively. 7 mol % of Tb3+ doped strontium aluminate was found more suitable for the production of green light component of WLED and for display applications. These compounds were also showed better visualization capabilities to view the LFPs on different porous and nonporous surfaces. Nanopowders were strongly adsorbed by the surfaces which help to visualize the various ridge features including sweat-pores without any background hindrance. UV light of 254 nm wavelength exhibited the clearly visualized symbols with intense green light used for anti-counterfeiting.

Response surface methodology for optimization of medium components for extracellular protease production by Enterococcus faecalis InaCC B745

Enterococcus faecalis InaCC B745 was isolated from dadih, a traditional fermented milk from Bukittinggi, West Sumatra. Previous study shows that the lactic acid bacteria was able to produce protease. In this study, protease production was optimized using response surface methodology (RSM) with central composite design (CCD). The total of four variables and five-level combinations namely: 0-4% (w/v) of skim milk, 0-2 % (w/v) of yeast extract, 0-2 % (w/v) of glucose, and 0-2 % (w/v) of CaCO3. Statistical analysis showed that the production of protease by E. faecalis InaCC B745 is significantly influenced by skim milk, yeast extract, glucose, and CaCO3, combination of skim milk and yeast extract. The final concentrations of the optimized medium were 3% (w/v) skim milk, 1.5 % (w/v) yeast extract, 0.89 % (w/v) glucose, and 1.5 % (w/v) CaCO3. Under these conditions, the model predicted of protease activity was 60.637 U/ml or around 57.3% increased.

Effects of Moisture Content and Drying Method on Shelf Life and Quality of Coconut Residue

In this article, the quality changes and shelf life of by-products (coconut residue) obtained after coconut milk extraction were studied using three drying technologies: hot air drying, fluidized bed drying, and flash drying. Among the drying technologies, flash drying was most suitable for fresh coconut residue. It gave the least moisture content with the most stability of whiteness and oil content and the highest drying rate. Dried coconut residue using flash drying has the longest shelf life up to the 140th day with slight changes in the quality when stored in warehouse conditions of 35±1.0oC and 60-75% relative humidity. In terms of specific energy consumption, flash drying required the lowest energy of 8.47±0.50 MJ per kg water removal from fresh coconut residue, whereas hot air drying consumed the highest specific energy.

Surface-Enhanced Raman Spectroscopy for Bisphenols Detection: Toward a Better Understanding of the Analyte–Nanosystem Interactions

Silver nanoparticles functionalized with thiolated β-cyclodextrin (CD-SH) were employed for the detection of bisphenols (BPs) A, B, and S by means of surface-enhanced Raman spectroscopy (SERS). The functionalization of Ag nanoparticles with CD-SH leads to an improvement of the sensitivity of the implemented SERS nanosensor. Using a multivariate analysis of the SERS data, the limit of detection of these compounds was estimated at about 10−7 M, in the range of the tens of ppb. Structural analysis of the CD-SH/BP complex was performed by density functional theory (DFT) calculations. Theoretical results allowed the assignment of key structural vibrational bands related to ring breathing motions and the inter-ring vibrations and pointed out an external interaction due to four hydrogen bonds between the hydroxyl groups of BP and CD located at the external top of the CD cone. DFT calculations allowed also checking the interaction energies of the different molecular species on the Ag surface and testing the effect of the presence of CD-SH on the BPs’ affinity. These findings were in agreement with the experimental evidences that there is not an actual inclusion of BP inside the CD cavity. The SERS sensor and the analysis procedure of data based on partial least square regression proposed here were tested in a real sample consisting of the detection of BPs in milk extracts to validate the detection performance of the SERS sensor.

ANTIOXIDANT, SEDATIVE AND HYPNOTIC POTENTIAL OF MILK EXTRACT OF MYRISTICA FRAGRANS: A COMPLIMENTARY MEDICINE FOR THE MANAGEMENT OF INSOMNIA

The Myristica fragrans (MF) is well known in Ayurveda for many therapeutic uses. The present research work was carried out to investigate the antioxidant (in vitro), sedative and hypnotic effects of the MFmilk extracts in Wistar rats. The extraction was carried out by hot percolation and cold maceration methods. The phytochemical, HPLC and pharmacological evaluations were done. The overall sedative and hypnotic effects of the different extracts were evaluated using the parameters locomotor activity, muscle strength and thiopental induced sleeping time. The milk extracts shown the presence of quercetin. It was confirmed using HPLC analysis, which might be responsible for its in-vitro antioxidant activity. The extract also showed the significant (P<0.05) sedative and hypnotic effect at 50 mg/kg, 100 mg/kg and 200 mg/kg on oral administration. Consequently, the treated groups have shown a significant increase in thiopental induced sleeping time. The study concluded that the milk extracts possess antioxidant, sedative and hypnotic activity. further research is needed to develop suitable formulation and to establish the mechanism of action of milk extract of MF.

Telelactation with a Mobile App: User Profile and Most Common Queries.

Background: Mobile applications related to health issues are currently expanding. Different uses of new technologies have produced positive results regarding breastfeeding support. Breastfeeding applications are increasing. Objective: We conducted a descriptive analysis of a mobile application for breastfeeding (LactApp) to study the user profile and the most frequent queries. Materials and Methods: This was a retrospective, comparative, and descriptive ecological time-series study of LactApp from 2016 to 2019. Google Analytics and the app itself were used for data collection. The data were analyzed in Excel, and for the time series, Prais-Winsten autoregressions were applied based on the Durbin-Watson method in Stata. Results: A total of 115,830 users and 71,780 infants were registered in the application. A total of 1.91% of these users obtained the medical version. The application was used for both queries and surveys and for users to interact through chat. A total of 30.17% of the responses were related with "baby's sleep" (8.94%), 8.91% were related to "preservation of milk," 6.16% were related to "breastfeeding crisis," and 6.15% were related to "physiological evolution of breastfeeding," all with an increasing trend. Conclusion: LactApp is a resource for breastfeeding that is widely downloaded and used by a substantial number of individuals. The most recurring topics were baby's sleep, milk extraction and preservation, breastfeeding crisis and physiological evolution of breastfeeding.

Comparison of Antioxidant Compounds between Glycine max and Bos taurus milk

Milks are dairy products that consists of high nutritional value which is good for health and provides energy to the body. Besides, milk also is believed to have antioxidant properties which helps to reduce the oxidative stress that may cause many diseases. Generally, cow milks are the most popular amongst all the dairy products as it is affordable and easy to find. However, dairy products that are originated from cow contains protein that can induce allergen reactions to some people. Thus, there is milk produced from the plant-based which is soybean that can be used to replace cow milk as it is believed to have similar nutritional value to cow milk. Therefore, to prove its similarity, an identification of antioxidant compounds was performed on soybean milk and cow milk extract using Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. The extraction was done by using liquid-liquid extraction method and the excess solvent was removed by using rotary evaporator machine. As a result, soybean milk has been identified to have the similar antioxidant compounds to cow milk. It can be proven with the presence of functional group that are similar to tryptophan and tyrosine which are both existed in cow milk. Hence, this research is essential for community as it contribute maximum benefit for better healthcare.

Analysis of Fatty Acids in Breastmilk During a 9-month Lactation Period Using Gas Chromatography–Mass Spectroscopy (GC-MS)

Breastmilk is very much needed to meet the needs of babies in every way because it contains important nutrients, carbohydrates in the form of lactose, and fatty acids in the form of polyunsaturated fatty acids. Research on fatty acid content in breastmilk with a lactation period of 9 months is needed to provide information to the public related to fatty acid content of breastmilk during 9 months lactation. The study is to analyze fatty acid content in the form of saturated fatty acids or unsaturated fat contained in breast milk. Breastmilk taken in the study was as much as 50 ml in a 250 ml erlenmeyer solution. To the sample 50 ml of concentrated, HCL concentration was added as much as 5 m and then the solution was beat for 15 minutes and was exposed ultrasonic therapy for 1 hour. The solution was then extracted using n-hexane and aquabidest solution until the milk oil solution was separated. Milk oil was extracted by adding 50 ml of Na2SO4 1 m then deciphere was performed. The breastmilk milk extraction oil was weighed 0.1 gram in a Teflon-filled reaction tube. A 15% BF3 solution in methanol was added 0.5 ml and then was heated in an air bath with a temperature of 45 ° C for 30 minutes. After cooling, solution of 0.2 ml of n-hexane was added to form two layers. The upper layer which was fatty acid methyl ester was taken by using syringe and then was injected in GC-MS. In this study, the total saturated fatty acid was 42.54% and the total unsaturated fatty acid was 57.17%. Saturated fatty acids consisted of lauric, myristic, palmitate, and stearate. Omega-6 fatty acids and monounsaturated fatty acids consisted of omega-9 fatty acids. In Mother's Milk with 9 months lactation period there are various fatty acid content of both saturated fatty acids, monounsaturated fatty acids (omega-9) and polyunsaturated fatty acids (omega-6).

Effect of foliar application of fungicides, Neem extract and Butter Milk on yellow rust, plant growth and yield parameters of wheat

Ever dynamic Puccinia striiformis f.sp. tritici, evolving into new virulences has led to frequent breakdown of resistance in wheat varieties in Punjab. Therefore, timely and need based application of fungicides to manage yellow rust of wheat is very important. Azoxystrobin + difenoconazole 325 SC, azoxystrobin + cyproconazole 280 SC, azoxystrobin + tebuconazole 320 SC, pyraclostrobin + epoxiconazole 175 SE, tebuconazole 25 EC each @ 0.1%, captan + hexaconazole 75 WP@ 0.3% and trifloxistrobin + tebuconazole 75 WG @ 0.6% proved highly effective and gave almost complete control of yellow rust and inhibited sporulation in trials conducted during 2016–17 and 2017–18 crop season. An increase of up to 30.2 per cent in thousand grain weight and 40.2 per cent in grain yield was observed with these fungicidal treatments. Improved plant growth attributes like tillering and plant height was also observed along with disease control. The efficacy of neem leaf extract, fermented butter milk and neem leaf extract + fermented butter milk was low as compared to fungicides. However, being indigenous home production, it resulted in highest cost-benefit ratio i.e. 1: 11.86. Maximum thousand grain weight of 45.6g and yield of 57.03q/ha was observed in azoxystrobin+difenoconazole 325 SC, which was 30.2 and 40.3 per cent more than control, respectively.

Benefits and safety of the use of devices accessories to milk extraction: literature review

Benefits and safety of the use of devices accessories to milk extraction: literature review

Physicochemical properties of human breast milk during the second year of lactation

The present study examined the microstructure as well as the physicochemical properties of human milk during the second year of lactation in an attempt to explore its applicability for the formulation of food products. It was observed that human milk fat globules (MFG) droplet size increased within 3 days of milk extraction due to coalescence, as evidenced by confocal microscopy. Furthermore, a gradual decrease of the average MFG size was noted from the sixteenth (16th) to twenty-fifth (25th) month of lactation. It was also found that the size of casein micellar structures increased upon acidification to pH 4.3 (isoelectric point of human caseins). In addition, human milk proteins enhanced the stability of oil-in-water emulsions against coalescence compared to cow, sheep, and goat milk proteins employed as macromolecular emulsifying ingredients. The cold-acid-gels of human milk proteins showed a less elastic behavior than the other milk samples, possibly due to the different structure, composition and size of human casein micelles. Furthermore, the DSC thermograms showed that human whey proteins are denatured in the same temperature range as do the cow whey proteins, but exhibit different thermal transition profiles. Overall, the findings of this research confirm that both the structure and the physicochemical properties of human milk are affected by the stage of lactation. Moreover, the particular composition and structure of human milk proteins seem to be responsible for the special functional characteristics of human milk that may lead towards the formulation of innovative products.

Comparison of Antioxidant Compounds between Glycine max and Bos taurus milk

Milks are dairy products that consists of high nutritional value which is good for health and provides energy to the body. Besides, milk also is believed to have antioxidant properties which helps to reduce the oxidative stress that may cause many diseases. Generally, cow milks are the most popular amongst all the dairy products as it is affordable and easy to find. However, dairy products that are originated from cow contains protein that can induce allergen reactions to some people. Thus, there is milk produced from the plant-based which is soybean that can be used to replace cow milk as it is believed to have similar nutritional value to cow milk. Therefore, to prove its similarity, an identification of antioxidant compounds was performed on soybean milk and cow milk extract using Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. The extraction was done by using liquid-liquid extraction method and the excess solvent was removed by using rotary evaporator machine. As a result, soybean milk has been identified to have the similar antioxidant compounds to cow milk. It can be proven with the presence of functional group that are similar to tryptophan and tyrosine which are both existed in cow milk. Hence, this research is essential for community as it contribute maximum benefit for better healthcare.

Effects of condensed tannin-amended cassava silage blend diets on feeding behavior, digestibility, nitrogen balance, milk yield and milk composition in dairy goats

Condensed tannins (CTs) are phenolic compounds derived from secondary plant metabolism that act as part of the plant's chemical defense system against pathogen invasion and herbivorous attack. This study aimed to evaluate the intake, digestibility, nitrogen (N) balance, production and composition of milk from goats fed cassava silage with added levels of CTs. Eight Anglo-Nubian goats with a mean BW of 40 ± 2.0 kg were distributed in a double Latin square design with four levels of CTs (0, 25, 50 and 75 g/kg DM) with four 20-day periods with 15 days of adaptation and five evaluation days for each period. No differences were observed in DM, NDF, CP intake and feed conversion (grams of DM intake (DMI) per gram of milk produced); however, when expressed as percent of BW, DMI showed a quadratic increase to 29.1 g/kg. As the level of supplemented CTs increased in the diet, the CP digestibility (P = 0.023), NDF (P = 0.044), non-fiber carbohydrates (NFC; P = 0.032) and total digestible nutrients (P = 0.033) exhibited a linear decrease. Furthermore, the addition of CTs to cassava silage induced a linear increase in N-fecal excretion (P = 0.014) and a positive quadratic effect on N-retained (P = 0.014) and N-balance (P = 0.024) as well as a positive quadratic trend in N-digested (P = 0.092). Milk urea N (P = 0.023) decreased linearly. The addition of CTs to cassava silage had a positive quadratic effect on ruminating time (P = 0.011). In addition, comparing the use or non-use from the orthogonal contrast test, the inclusion of CTs in goat diet increased water and N-intake, CP and NDF digestibility, spent time eating and ruminating and N-balance and decreased milk production corrected3.5%, fat milk content, milk urea N and dry defatted extract of milk. Thus, adding CTs to cassava silage at 25 g/kg total DM promoted goats' greater use of the diet without impairing feed conversion and the quality of goat milk produced. Dietary levels of 50 and 75 g/kg total DM are not recommended because under the conditions of this study, they reduced the productive efficiency of dairy goats.

Physico-chemical properties of Paneer prepared from blends of raw turmeric extract and buffalo milk

Turmeric is a spice that has received much interest from both the medical/scientific worlds as well as from the culinary world. Turmeric has nutritional and health benefits of curcumin and it has been traditionally used for centuries in Asian cuisine. In present investigation an attempts was made to prepare paneer by using buffalo milk and raw turmeric extract and studied its compositional parameters. The paneer was prepared by considering treatment combination of buffalo milk and raw turmeric extract as 95%, 90% and 85% of buffalo milk and 5%, 10% and 15% of raw turmeric extract in treatments T2, T3 and T4 and treatment T1 taken as a control prepared from buffalo milk only. The compositional parameters include acidity, pH, fat, protein, moisture, total solid, ash were studied. It was observed that as the raw turmeric extract increase decreased the acidity of the developed product. This effect may be due to alkaline nature of turmeric.

Nanoplasmonic Paper-Based Platform for General Screening of Biomacromolecules.

Hygiene assessment in industrial and clinical environments is crucial in the prevention of health risks. Current technologies for routine cleanliness evaluation rely on the detection of specific biomolecules, thus requiring more than one test for broad-range screening. Herein, the modulation of the catalytic activity of gold nanoparticles (AuNPs) by biomacromolecules was employed to develop a nanoplasmonic platform for general hygiene screening. AuNPs were immobilized on cellulose paper by simple adsorption. When ferricyanide was dispensed onto the paper, the AuNPs catalysed the ferricyanide’s dissociation, releasing free cyanide ions that dissolved them. The AuNP dissolution produced an intense colour shift detectable with the naked eye. When biomacromolecules (e.g., proteins and polysaccharides) were present, they spontaneously attached to AuNPs, forming a biomolecular corona (biocorona), reducing their catalytic activity until complete suppression when the NPs were fully covered by molecules. The concentration-dependent decrease in the catalytic activity was here used to quantify biomacromolecules and complex samples such as milk, eggs, soy sauce and yeast extract (in 20 min), with detection limits comparable to those of standard methods, i.e., 0.25 µg mL−1 for albumin. This nano-enabled technology may be applied as a broad-range (unspecific) alert system for inexpensive cleanliness evaluation, with potential applications in sensitive sectors including productive industries and hospitals.

Breastfeeding and factors associated to feeding in babies with cleft palate

Breastfeeding can be affected in babies with cleft palate, due to various factors associated with feeding. The objective of the study was to relate the time of breastfeeding and the time to express breast milk with factors associated with feeding in babies between 0 and 6 months with cleft palate. Electronic records of care of 36 babies with cleft palate from a public hospital in Chile were reviewed. Sociodemographic characteristics of the mother and father, perinatal and postnatal antecedents of the babies and general characteristics of feeding up to 6 months were extracted. The data were compared with the Mann-Whitney U test and relationships were established with the Pearson and Spearman correlations. In the time of direct breastfeeding (days), significant differences were observed in: the babies who used a nasogastric tube (p = 0.009), the indication to express breast milk (p = 0.017), the presence of manual extraction of breast milk (p = 0.005) and the presence of electrical extraction of breast milk (p = 0.002). In addition, a positive correlation (p = <.001; r = 0.538) was recorded between the time of direct breastfeeding and the time of expressed breast milk. It is concluded that the longer the breastfeeding time, the longer the breast milk extraction time. Meanwhile, the time of breastfeeding was influenced by the use of a nasogastric tube, the expression of breast milk as a personal option, and the manual and electrical expression of breast milk.