The study aims to detect the active ingredients in the plant and to know their biological effectiveness against bacteria. The pH and humidity of the plant were measured. Aqueous and alcoholic extracts were prepared to obtain the crude extract in order to estimate its effectiveness against bacteria. The study showed the effectiveness of henna leaves (Lawsonia inermis) and their inhibition of the growth of some types of tested bacteria, including Staphylococcus, Strep Pyogenes, Escherichia Coli, salmonella, and Shigella. The inhibition results of the extracts varied and depended on the extraction method, temperature, and solvent used. The results of the initial chemical examination also showed that these extracts contained some important active ingredients such as (tannins, saponins, flavonoids, glycosides, resins, alkaloids), which made this plant used as a treatment for infections. The pH was estimated, and thus the importance of this plant increases as it can be used as a natural antibiotic. Therefore, we recommend studying the quantity and quality of the active compounds and knowing their molecular structure with the aim of developing natural extracts and dispensing with manufactured compounds due to the harm they cause to the living organism. Keywords: henna plant, qualitative findings, aqueous and alcoholic plant extracts, some types of pathogenic bacteria