Pomegranate Peel Extract Research Articles

The effect of pomegranate peel extract and apple cider vinegar as an irrigants in disinfecting the necrotic primary root canal

This in-vivo study aims to assess pomegranate peel extract (PPE) and Apple Cider Vinegar (ACV) efficacy in disinfecting the necrotic primary teeth root canals. Forty-five primary incisors in thirty children. The treatment was performed under local anesthesia with rubber dam isolation. Sterile paper points were inserted for 60 s to collect the first bacterial swab (S1). Root canal walls were prepared and irrigated with 22 mL within 5 min according to the following groups (randomly with 1:1:1 allocation ratio): Group A: (n = 15) with 5% PPE, Group B: (n = 15) with 5% ACV, and Group C: (n = 15) with 5% sodium hypochlorite (NaOCl). Canals were dried with paper points, and paper point was placed in each canal for 60 s to collect the second bacterial swab (S2). Bacterial colonies were counted after 48-hour incubation at 37 °C and the bacterial counts in the S1 and S2 for each sample were compared using the decimal logarithm. Intra-group comparisons were made using Paired sample t and Wilcoxon signed ranks tests. Between-groups comparisons were made using One-Way ANOVA, Kruskal-Wallis, Mann-Whitney U, and Independent Samples tests. The significance level was set at α = 0.05. PPE demonstrated a 60.43% reduction in CFU count, ACV demonstrated a 51.56% reduction in CFU count, while NaOCl showed an 87.49% reduction in CFU count. Although NaOCl has shown effectiveness in reducing bacterial counts, PPE and ACV have also displayed some antibacterial activity. However, their overall efficacy is less established, which raises questions about their viability as a natural alternative to a 5% NaOCl.

Laser irradiation and pomegranate peel for preservation of the strawberry quality

The present study is aimed to explore the influence of laser irradiation and coating with chitosan and pomegranate peel extract on the physico-chemical properties, weight loss, firmness, functional, microbiological, color, and sensory characteristics of strawberry for fifteen days of cold storage. Four treatments of strawberry were conducted with laser irradiation for 6 min. The second, third, and fourth groups were coated with chitosan, pomegranate peel extract (PPE), and mixture of chitosan and PPE at ratio 1:1. Compared to untreated strawberries, the exposure to laser radiation decreased the weight loss of strawberries by 43.44%. Also, the laser irradiation reduced the negative effect of storage on the total phenolic content, anthocyanin, ascorbic acid, and antioxidant ability of strawberry. The exposure of strawberry to laser irradiation reduced the counts of fungi and psychrotrophic bacteria and provided the positive effect on the color and sensory attributes. The coating with PPE and chitosan enhanced the positive effect of laser irradiation on the functional and quality properties of strawberry during its cold storage. It can be concluded that laser irradiation and coating with PPE may be applied as novel techniques for the preservation of strawberry properties during its storage period.

Oxazolone-Induced Immune Response in Atopic Dermatitis Using a Goat Model and Exploration of the Therapeutic Potential of Pomegranate Peel Extract

Atopic dermatitis (AD) remains a challenging condition, necessitating effective models for investigation and therapeutic exploration. In this study, we employed a goat skin model to assess the immune response triggered by topical oxazolone (OX) application. The OX simulates a Th2-driven immune reaction typified by elevated Th2 cytokine (IL-4 and IL-13) expression and the infiltration of mononuclear cells magnified by the presence of CD3+ and CD4+ T-cells in the epidermal and dermal layers. Additionally, the application of pomegranate peel extract (PPE) demonstrated a mitigating effect on OX-induced skin alterations, suggesting potential therapeutic benefits. This study underscores the value of goats as a reliable and accessible model for studying AD in humans and/or large animals. While the therapeutic potential of PPE dosage requires further exploration.

Bacterial nanocellulose and its oxidized form as functional carriers for pomegranate peel extract: A sustainable approach to bioactive delivery

Bacterial nanocellulose and its oxidized form as functional carriers for pomegranate peel extract: A sustainable approach to bioactive delivery

Bidirectional approach of Punica granatum natural compounds: reduction in lung cancer and SARS-CoV-2 propagation

The spreading of COVID-19 has posed a risk to global health, especially for lung cancer patients. An investigation is needed to overcome the challenges of COVID-19 pathophysiology and lung cancer disease. This study was designed to evaluate the phytoconstituents in Punica granatum peel (PGP), its anti-lung cancer activity, and in silico evaluation for antiviral potential. GC–MS technique was used to detect the phytoconstituents. Cytotoxicity was analyzed using MTT dye, followed by apoptosis, ROS generation, and cell cycle phase detection in human lung cancer cells (A549). The glide module of Maestro software was used to investigate the molecular-docking interaction of the constituents against main protease (Mpro) and papain-like protease (PLpro) of SARS-CoV-2. GROMACS 2023.2 was utilized to evaluate the complex stability. A total of nineteen phytocomponents were detected in the PGP extract through GC–MS analysis. PGP has shown a potential to reduce lung cancer cell proliferation while evading normal cell death. PGP induced apoptosis by arresting cells in the G0/G1 phase and generating ROS. A total of six and eight phytocomponents had a high affinity for PLpro and Mpro proteins, respectively. The top docked complex, ethyl 5-oxo-2-pyrrolidinecarboxylate, with PLpro and Mpro proteins, showed likely stable interaction throughout 100 ns simulation. This finding raises the possibility of top-eight hits (docking score ≥ -1.0 kcal/mol) preventing SARS-CoV-2 severity. The phytoconstituents exhibited orally active drugs with no more than one violation and drug-likeness activity. The PGP phytoconstituents are suggested to be dual agents for lung cancer and SARS-CoV-2 pathogenesis.Graphical

Pharmacodynamics, pharmacokinetics, and cell signaling pathways of pomegranate peel extract

Pharmacodynamics, pharmacokinetics, and cell signaling pathways of pomegranate peel extract

Soy Protein Isolate Edible Coating Incorporated with Pomegranate (<i>Punica granatum</i> L.) Peel Extract: Effect on Quality of Fresh-Cut Pineapples

This study investigated the development of an edible coating using soy protein isolate (SPI) and pomegranate peel extract (PPE) to extend the shelf life of fresh-cut pineapple (FCP). Different dipping times (0, 2, 3, and 4 min) of the FCP in the coating solution were evaluated, and the samples were stored at 4 °C for 15 days, with analyses performed on days 0, 3, 8, 10, and 15. Results showed that the coated FCPs exhibited higher firmness and titratable acidity. Furthermore, the PPE-SPI coating also prevented weight loss as well as delayed the increase in pH and total soluble solids, suggesting slower senescence and enhanced preservation. Notably, a 4 min dipping time significantly (p < 0.05) reduced total plate count and yeast and mould count compared to control samples by 30% and 40%, respectively. The study suggests that longer dipping times enhance the effectiveness of the PPE-SPI coating in extending the shelf life of FCP, showcasing the potential of the PPE-SPI coating as an alternative to synthetic preservation methods for fresh-cut fruits.

Physalis peruviana calyces extract ameliorate oxidative stress, inflammation, and immune loss in rats-exposed to hexaflumuron

BackgroundHexaflumuron (HFM), a common pesticide, can disrupt the immune system and cause oxidative stress. This study investigated the potential of Physalis peruviana L. calyces extract (PP) to counteract these effects in rats.MethodsRats were divided into 6 groups including control, PP-treated, HFM-exposed, and co-treated (HFM + PP) groups. Immune function, antioxidant activity, and organ damage were assessed. Furthermore, UPLC-MS/MS analysis identified potential bioactive compounds in PP extract.ResultsHFM exposure suppressed immune responses and caused organ damage. Notably, the co-administration of PP extract with HFM reversed these effects, indicating its ability to reduce oxidative stress and protect the immune system. UPLC-MS/MS analysis of PP calyces ethanolic extract revealed its richness in various health-promoting metabolites, including acyl sucrose sugar, withanolides, and flavonoids, which may provide valuable insight into the underlying mechanisms of PP’s calyces protective effects against HFM toxicity.ConclusionsThis study provides novel insights into the potential of P. peruviana L. calyces ethanolic extract as a natural agent to counteract the harmful effects of HFM exposure. These findings have significant implications for developing effective strategies to mitigate pesticide-induced toxicity and promote human health.

Dry crude pomegranate peel extract as a bio-input for medicinal pharmaceutical gel with healing activity.

The sustainable use of pomegranate peel, a by-product of the food industry, is gaining importance in developing pharmaceutical bio-inputs, aligning with circular economy practices and waste reduction. This study explores the application of dry crude pomegranate peel extract (PPE) as a bio-input for medicinal gels with wound healing properties. PPE was extracted via percolation in ethanol and freeze-dried. High-performance liquid chromatography (HPLC) and mass spectrometry identified key bioactive compounds: gallic acid, punicalagin, punicalin, ellagic acid, and citric acid, recognized for their antioxidant, antimicrobial, and healing properties. In vitro assays revealed low hemolytic activity, non-cytotoxicity, significant fibroblast proliferation, and robust antioxidant activity (95.99% DPPH scavenging at 100 µg/mL). A carbomer gel containing 2.5% w/w of the extract effectively healed wounds in rats, with performance comparable to silver sulfadiazine ointment, while demonstrating no microbial contamination during the process. These findings position PPE as a promising, sustainable, and effective alternative for wound care pharmacotherapy, addressing both medical needs and environmental sustainability.

Effects of Diets Containing Extruded Linseed and Padina pavonica Algae on Meat Rabbit: Carcass Performance and Meat Quality.

This study investigated the effects of dietary supplementation with extruded linseed (ELS) and Padina pavonica algae extract (PP) on rabbit carcass and meat quality. Ninety-six rabbit carcasses from two production cycles were analyzed. In the first cycle (C1), rabbits were fed a control diet (1CNT), the same diet supplemented with 5% ELS (1ELS5%), and supplemented with 3.5% ELS and 0.2% PP (1LPP3.5%). In the second cycle (C2), the diets varied in composition and supplementation levels: a different control diet (2CNT), the same diet with 5% ELS (2ELS5%), and with 5% ELS and 0.2% PP (2LPP5%). Meat analyses were performed on Longissimus thoracis et lumborum (LTL) muscle for physical properties and on thigh meat (THM) for proximate composition, vitamin E, coenzyme-Q10, cholesterol, fatty acid profile, and mineral content. No significant differences in LTL physical quality were observed in C1, although LTL was brighter in C2 (p < 0.001). THM in C2 had higher fat content (p < 0.001). Dietary supplementation with ELS and PP extract significantly increased polyunsaturated fatty acids (n-3 PUFAs) and improved the n-6/n-3 ratio (p < 0.001) in rabbit meat, demonstrating their positive impact on meat quality.

The Synthesis of Functionalized W5O14 Nanorods for the Adsorption of Bismarck Brown R from Wastewater

In this research, a tungsten oxide was prepared via a green (biogenic) synthesis route where sodium tungstate dihydrate and Punica granatum peel extract were used as a precursor and a reducing/capping agent, respectively. The characterization of the prepared tungsten oxide was performed through various spectroscopic and microscopic techniques. The characterization results revealed the preparation of highly crystalline and nanorod-shaped (length = 123 nm and width = 31.3 nm) tungsten oxide with a probable chemical formula of W5O14. Various functional groups on the W5O14 surface were also reported. The prepared nanorods were further used for the removal of Bismarck Brown R (BBR) dye from water in a batch manner. By varying the dose of nanorods (0.5–3.0 g L−1), BBR solution pH (2−10), contact time (15–120 min), BBR concentration in solution (10–60 mg L−1), and temperature of BBR solution (30, 40, and 50 °C), the optimized condition for maximum adsorption efficiency was measured. The results revealed that 2.0 g L−1 amount of nanorods of tungsten oxide were used to remove ~98% of BBR dye from its 10 mg L−1 at 30 °C and 7.0 pH. The temperature-dependent adsorption data were fitted to different types of non-linear isotherm models (e.g., Langmuir and Freundlich) to assess the adsorption potential and adsorption mechanisms in relation to temperature impacts. The synthesized nano-adsorbent fits the Langmuir as well as the Freundlich isotherm model with a maximum adsorption capacity of 17.84 mg g−1. Pseudo-first-order, pseudo-second-order, and Elovich kinetic models were used for the study of adsorption kinetics. BBR adsorption onto the W5O14 nanorods follows the pseudo-second-order rates. The present adsorption is governed by physico-chemical adsorption with predominant chemical interactions.

Inhibition of Salmonella enterica and Enterohemorrhagic Escherichia coli by Ethanolic Extracts of Pomegranate Peels

This study compared the effect of phenolics extracted from four different types of pomegranate peels for controlling the growth of Salmonella enterica and enterohemorrhagic Escherichia coli. Cells of the two bacterial cultures (5 log CFU/mL) were treated in tryptic soy broth containing 0, 1, or 2% ethanolic extracts of four pomegranate peels for 5, 10, or 24 h at 25 °C. The surviving cells were recovered on a general-purpose agar and a selective medium. The extracts of three products were more effective (p ≤ 0.05) against E. coli than the extract of the remaining product, which had a similar activity to the extracts of two of the products used against S. enterica. Longer treatment time and higher extract concentration resulted in greater pathogen population reductions. The bacterial strains used had varied susceptibility to the extracts. Reductions in cell population positively correlated with the total contents of hydrolyzable tannins in the treatment systems. These findings suggested that ethanolic extracts of evaluated pomegranate peels had inhibitory activities against the two bacterial pathogens. This highlights the potential of pomegranate peels as a promising natural alternative to conventional antimicrobials for controlling foodborne pathogens like S. enterica and E. coli.

Anti-Inflammatory, Antioxidant and Antibacterial Properties of Tomato Skin and Pomegranate Peel Extracts: A Sustainable Approach for Oral Health Care.

Agricultural food waste and by-products could provide high-value compounds that positively affect human and environmental health, thus representing promising ingredients for cosmeceutical products. This study explores the biological activities of tomato skin (HP) and pomegranate peel (PPE) extracts on oral mucosa to evaluate their possible use in mouthwashes. The biological activities of the extracts and the mouthwash (MW) containing them were evaluated in Human Primary Gingival Epithelial cells (HGECs). The antioxidant and anti-inflammatory activities were analyzed in HGECs injured with lipopolysaccharides. After 24 h of treatment with PPE, HP, and MW, significant antioxidant activity and an increased Superoxide Dismutase 1 expression (p < 0.01) were observed. Additionally, the extracts significantly reduced the expression of tumor necrosis factor α (p < 0.05) and Monocyte Chemoattractant Protein 1 (p < 0.001), suggesting an anti-inflammatory role. Lastly, the antibacterial activity was assessed against Streptococcus mutans and Streptococcus sanguinis by the broth microdilution method and agar cup diffusion test for the extracts and the mouthwash, respectively, demonstrating strong effectiveness against both oral streptococcus species. Results demonstrate the potential of HP and PPE in reducing oxidative stress, inflammation, and bacterial proliferation within oral mucosa, highlighting food waste up-cycling as a resource for human health.

Green synthesis of iron nanoparticles to promote seed germination of Zea mays under salinity condition.

The application of green synthesis of nanoparticles in agriculture serves as an environmentally benign strategy. Pomegranate Peel extract (PPE) was utilized to produce iron nanoparticles (Fe NPs). PPE contains biomaterials that have the ability to synthesize Fe NPs through the reduction the iron precursor salt (FeSO4•7H2O) and also act as capping and stabilizing agents. UV-Visible, FTIR, XRD, SEM, EDX, and nano zeta-potential analysis were used to investigate Fe NPs. The synthesis of Fe NPs was confirmed by a significant color change from yellow to black. Then Fe NP production is confirmed by a UV-Visible peak at 301nm. FTIR showed O-H and C=C stretching due to phenol and alkene functional groups. X-ray diffraction showed that Fe NPs are mostly amorphous. The synthesized Fe NPs were spherical and have around 12nm in size, with a 5.5mV value, the synthesized Fe NPs were stable. The effects of these (Fe NPs) on the germination of maize (Zea mays L.) labeled Z1(Buhooth 5018), Z2(Baghdad-3) and Z3 (Fajr-1) seeds and the growth of their roots were assessed under conditions of salinity at different levels (0, 8, 12dsm-1) in a laboratory-scale system. The findings indicated that the application of the synthesized Fe NPs at a concentration of 20Mm caused an increase in germination ratio, root and shoot length, seedling vigor index, root length stress tolerance index (RLSTI) and shoot length stress tolerance index (SLSTI). The findings showed that the stress-relieving benefits of Fe NPs were more effective than normal forms, which may be related to their distribution, shape, size, and other properties. In general, the results of the present research indicate that utilizing pomegranate peel extract for eco-friendly production of nanoparticles could enhance the germination of seeds and the strength of seedlings in Zea mays.

Assessing the antioxidant potential and antibacterial properties of pomegranate peel extracts from Wonderful and Valenciana varieties.

Recent studies have found a high content of polyphenolic compounds in the fruit produced by pomegranate trees (peel, arils, seeds, and capillary membranes). Most polyphenols are generally accepted to be potent antioxidants with anti-inflammatory properties. Pomegranate peel is considered a waste product of the human diet. This value is equivalent to 50% of the weight of the fruit, which is why we propose a comparative analysis of both its antioxidant and microbicidal activity in two of the most cultivated varieties in Mexico, Wonderful (sour) and Valenciana (sweet), to provide added value to products of the region by obtaining products of high biological value via a process of easy industrial scaling.

Facile fabrication of novel magnetic chitosan-alginate @ pomegranate peel extract nanocomposite for the adsorptive removal of naphthalene from aqueous solutions.

The prevalent presence of naphthalene contamination in aquatic ecosystems is a significant concern due to its carcinogenic and priority pollutant properties. This study focuses on the synthesis of magnetized chitosan/alginate/pomegranate peel extract nanocomposites (Fe3O4/PPE/Cs-Alg), was characterized by Zeta potential, vibrating sample magnetometer (VSM), Field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses. The results predicted that the successfully synthesis of Fe3O4/PPE/Cs-Alg. The study evaluates the efficacy of the nanocomposite in naphthalene removal, considering operational parameters like pH, varied initial naphthalene concentrations, nanocomposite doses and contact time . The maximum adsorption capacities of naphthalene optimal conditions were 88.12mg/g for 50ppm initial naphthalene concentration. The Langmuir, Freundlich, Temkin and Sips isotherms were applied to analyze the experimental equilibrium data. The Sips isotherm was identified as the most suitable model, as evidenced by the highest (R2=0.97), Also, the adsorption data conformed well to the pseudo-second-order kinetics model (R2=0.99). The thermodynamic study showed positive values for ΔH° and ΔS° throughout the adsorption process respectively, implying an endothermic behavior. Therefore, we found that it can significantly remove naphthalene in aqueous environments and hence could be useful for cleaning up the environment from Poly Aromatic Hydrocarbon.

Recent advances in encapsulation of pomegranate peel extract and combination of wall materials: a review of encapsulation technologies, characterization and applications in the food industry

Pomegranate peel comprises a vital source of bioactive constituents such as antioxidants, fiber, vitamins, and minerals.

Inhibitory Effects of Pomegranate Peel Extract against Staphylococcus aureus causing Animals Mastitis in Sohag Governorate

Inhibitory Effects of Pomegranate Peel Extract against Staphylococcus aureus causing Animals Mastitis in Sohag Governorate

Optimization of glycerol and cellulose nanofiber concentrations in Opuntia ficus-indica mucilage films functionalized with pomegranate peel extract for postharvest preservation of banana

Optimization of glycerol and cellulose nanofiber concentrations in Opuntia ficus-indica mucilage films functionalized with pomegranate peel extract for postharvest preservation of banana

In vitro evaluation of Punica granatum fruit peel extract for its potential anti-diabetic effects

Background: With growing awareness of pomegranate's health benefits, pomegranate products have been consumed more frequently in recent years, and pomegranate peel has emerged as one of the most prevalent wastes in the food industry. Pomegranate Peel concentrate is an indigenous substance with strong antioxidant and antidiabetic actions as a result of its tannins as well as polyphenols content. Methods: In the present study, pomegranate skin extract, both liquid and alcohol-based, was evaluated for polyphenolic and flavonoid content. Alcoholic fruit peel extract was also assessed for 1,4-α-D-glucan glucanohydrolase and α-D-glucoside glucohydrolase enzyme activity. Results: According to findings, pomegranate peel extract showed significant antioxidant content. Phytochemical analysis of ethanol-derived extract of pomegranate peel found a noteworthy amount of ellagitannins and flavonoids such as Punicalin, Punicalgin, Punicic acid, Catechin, Quercetin, Rutin, and Kaempferol. In contrast, punicalgin, ellagic acid, and gallic acid are responsible for antidiabetic activity. The LC-MS characterization of peel extract of pomegranate showed 10 bioactive compounds. The IC50 value for 80% alcoholic extract of pomegranate peel was found to be 5.86 mg/ml of α- amylase and 6.58 mg/ml of α-glucosidase. Conclusion: It was found that the inhibition of 1,4-α-D-glucan glucanohydrolase and α-D-glucoside glucohydrolase enzymes could be an effective mechanism by which it can give anti-diabetic effects.