Pine Needle Extract Research Articles

Isolation, characterization, and LC MS/MS determination of anti-obesity components from pine needles of Cedrus deodara (Roxb.) G. Don.

This study aimed to isolate and analyze the components in cedar pine needles (needle leaves of Cedrus deodara (Roxb.) G. Don) that exhibit anti-obesity effects, as determined through animal experiments. The extract of cedar pine needles was separated into four fractions of different polarities using a macroporous resin column. The fraction that retained anti-obesity activity was evaluated based on the results of animal experiments. Monomeric compounds were structurally characterized and isolated from the active fraction using a preparative liquid chromatography system. Combined with subsequent glucose gel chromatographic separation. The content of the separated components was determined using ultrahigh performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS). The water-washed fraction retained anti-obesity activity of the cedar pine needles more effectively. A total of 16 compounds were separated from this fraction, and the contents of 14 of these compounds were determined to be present in cedar pine needles. Nine components, namely p-hydroxy benzyl alcohol, chlorogenic acid, vanillic acid, syringic acid, P-coumaric acid, sinapic acid, benzoic acid, phenylacetic acid, salicylic acid, were characterized and determined for the first time in cedar pine needles. The components with anti-obesity activity in the pine needles of Cedrus are mainly derived from phenolic acids.

Comparative Antioxidant and Antimicrobial Activities of Several Conifer Needles and Bark Extracts.

Nowadays, an increased concern regarding using natural products for their health benefits can be observed. The aim of this study was to assess and compare several phenolic compounds found in 15- to 60-year-old Douglas fir, silver fir, larch, pine, and spruce needle and bark extracts and to evaluate their antioxidant and antimicrobial activities. Spectrophotometric assays were used to determine the total polyphenol content and the antioxidant activity that was assessed by using the DPPH• radical scavenging assay (RSA), the ferric reducing antioxidant power assay (FRAP), and the ABTS•+ radical cation scavenging assay (ABTS). The phytochemical content was determined by using high-performance liquid chromatography, and the antimicrobial activity was determined by assessing the minimal inhibition concentration (MIC). The results of the study show a total polyphenol content of 62.45-109.80 mg GAE/g d.w. and an antioxidant activity of 91.18-99.32% for RSA, 29.16-35.74 µmol TE/g d.w. for FRAP, and 38.23-53.57 µmol TE/g d.w. for ABTS. The greatest quantity of phenolic compound for most of the extracts was for (+)-catechin, and it had values between 165.79 and 5343.27 µg/g d.w. for these samples. The antimicrobial inhibition for all the extracts was the strongest for Staphylococcus aureus (MIC 62.5-125 µg/mL). The extracts analyzed could be used for their bioactive potential after further investigations.

여드름 개선용 화장품 소재 개발을 위한 솔잎 발효물에 대한 기능성 연구

In this study, the pine needles were fermented with microorganisms originated from
 Jeju island and the antioxidant and antibacterial activities of the fermented extracts were
 investigated to develop new cosmetic materials. The microorganisms used for
 fermentation were Bacillus coagulans KK7 strain, Lactobacillus brevis GJ26 strain, and
 Pediococcus acidilactici BDD10 strain isolated from Jeju. The antioxidant activities of the
 fermented and non-fermented pine needles extracts were evaluated by the DPPH radical
 scavenging activity. The DPPH radical scavenging activities of the fermented pine
 needles extracts were relatively higher than the non-fermented pine needles extracts. In
 order to examine whether the fermented pine needle extracts can be used as acne
 improvement materials, antibacterial tests were conducted on Cutibacterium acnes,
 Staphylococcus epidermidis and S. aureus. The antibacterial activities of the fermented
 and non-fermented pine needles extracts against C. acnes were confirmed, and the
 extracts of pine needles fermented by BDD10 strain showed better antibacterial activity
 compared to non-fermented pine needles extracts. These results suggest that fermented
 pine needles can be used as a new cosmetic material with antioxidant and antibacterial
 functions.

Simultaneous Determination of Three Flavonoids in Rat Plasma of Masson Pinus Needles (Pinus massoniana L.) Extracts by UHPLC-MS/MS and Application to Pharmacokinetics Studies after Oral and Transdermal Administration.

Pinus massoniana needles, a traditional herb, were applied to prevent hair loss in China. Studies available mainly focused on pine needle flavonoids with various biological activities. However, there has been no pharmacokinetics study of the flavonoids from Pinus needles extract. A selective and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to simultaneously quantify taxifolin, quercetin and catechin in rat plasma. To separate the three constituents, an Agilent Extend-C18 column (2.1mm × 100mm, 1.8μm) was used with a mobile phrase of (A) 0.1% formic acid and (B) acetonitrile. The analytes were measured by multiple reaction monitoring in the negative ionization mode. There was good linearity in the established UHPLC-MS/MS method, with a coefficient of determination (r2) of >0.99. The accuracy, intra-day and inter-day precision and recovery were all satisfactory and these 3 compounds were stable under the tested conditions. The validated method in this study was successfully applied to pharmacokinetic study in healthy rats after oral and transdermal administration of Pinus needles extract. The results could provide further research foundation for pine needle extract as external preparations.

Principle active metabolites of Pinus morrisonicola Hayata synergistically inhibit cell proliferation and autophagy to elevate apoptosis in hepatocellular carcinoma cells.

Hepatocellular carcinoma (HCC), a common primary tumor of liver is a leading cause of cancer-associated deaths. Improving cellular apoptosis and enhancing autophagic clearance is been considered to improve treatment outcomes of HCC. Polyphenols from Pinus morrisonicola (Hayata) have shown various physiological and therapeutic benefits and the flavonoid chrysin is been known for their anticancer effects. However, the main bioactive principle and the mechanism underlying the antitumor activity of pine needle extract are not clear yet. In this study, the effects of ethanol extract from pine needle on HCC cells were determined. The results show that when compared with administration of chrysin alone, a fraction containing pinocembrin, chrysin, and tiliroside significantly reduced autophagy and increased apoptosis. The results also correlated with decrease in cell cycle regulators and the autophagic proteins like LC3-II. Collectively, the results imply the fraction containing pinocembrin, chrysin, and tiliroside as an ideal complementary medicine for an effective antitumor activity.

Crosslinking methods for improving the properties of soy-protein based films for meat packaging: a review

Crosslinking methods have been used to improve the properties of soy protein-based films for various applications, such as meat packaging. Some of the crosslinking methods that have been reported in the literature include boiling soy milk, baking soy protein isolates, adding canola and sorghum proteins, incorporating Plantago major seed mucilage and Anethum graveolens essential oil, adding pine needle extract (PNE), incorporating montmorillonite and citric acid, using xylose as a crosslinker, and crosslinking with glutaraldehyde. The incorporation of additives such as canola and sorghum proteins, Plantago major seed mucilage and Anethum graveolens essential oil, and pine needle extract (PNE) has also been reported to improve the properties of soy protein-based films. In conclusion, soy protein-based films have excellent film-forming properties and many functional characteristics, making them a promising material for food packaging applications. However, their poor moisture barrier properties must be improved to make them more suitable for food packaging applications. Crosslinking methods have been used to improve the properties of soy protein-based films for various applications, such as meat packaging. The incorporation of additives such as canola and sorghum proteins, Plantago major seed mucilage and Anethum graveolens essential oil, and pine needle extract (PNE) has also been reported to improve the properties of soy protein-based films.

A novel film based on gluten, pectin, and polyphenols and its potential application in high-fat food.

A novel film based on some natural ingredients (wheat gluten, pectin, and polyphenols) was used to improve the quality and storage stability of high-fat food due to their good sustainable, mechanical, and edible properties. With the addition of polyphenols from Cedrus deodara (in the form of pine-needle extract (PNE)), the physicochemical properties (thickness, moisture content, and color), mechanical properties (tensile strength and elongation), barrier properties (water vapor, oil, and oxygen permeability, transmittance), and thermal stability of the composite film were improved. According to the analysis of infrared spectroscopy and molecular docking, the main compounds of PNE interacted with wheat gluten by hydrogen bonds and hydrophobic forces to form a compact and stable structure. In addition, the composite film showed a remarkable antioxidant capability to scavenge free radicals, and the film matrix could effectively protect the antioxidant activity of PNE. Furthermore, using cured meat as a model, the composite film exhibited a fine packaging performance in high-fat food during storage, which could obviously inhibit the excessive oxidation of fat and protein of cured meat and was beneficial in forming its special flavor. Our results suggest that the composite film possessed good properties and had potential for packing of high-fat foods, which could improve the quality and safety of food during processing and storage. © 2023 Society of Chemical Industry.

Effects of various pine needle extracts on Chinese hamster ovary cell growth and monoclonal antibody quality

Chinese hamster ovary (CHO) cells are commonly used as “bio-machines” to pro-duce monoclonal antibodies (mAb) because of their ability to produce very complex proteins. In this study, we evaluated the effects of pine needle water extract (PNWE), pine needle ethanol extract (PNEE), and pine needle polysaccharide extract (PNPE) on the CHO cell growth, mAb production and quality using a Fed-batch culture process. PNPE maintained high VCD and viability, and the titer increase was correlated with its concentration. Three extracts effectively reduced the acidic charge variant and modulated mAb glycosylation. PNPE had the most profound effect, with G0F decreasing by 8.7% and G1Fa increasing by 6.7%. The change in the glycoform was also closely related to the PNPE concentration. This study demonstrated that PNPE could facilitate CHO cell growth, increase the mAb production, decrease acidic charge variants, and regulate mAb glycoforms. To identify the components responsible for the above changes, the sugar and flavonoid contents in the extracts were determined, and the chemical compounds were identified by LC-MS, resulting in 38 compounds identified from PNPE. Rich in sugars and flavonoids in these three extracts may be related to increased CHO cell growth and productivity, and changes in glycoforms.

Application of Baltic Pine (Pinus sylvestris) Needle Extract as a Gut Microbiota-Modulating Feed Supplement for Domestic Chickens (Gallus gallus)

The valorization of wood industry residues is very desirable from a circular economy perspective. Pine needle extracts are known for their health-promoting properties and therefore can be used as herbal remedies and nutritional supplements. Since the withdrawal of antibiotics as growth promoters in the European Union, natural feed additives that improve poultry health and production are needed. It was proposed that pine needle extract could be a good alternative to antibiotic usage at sub-therapeutic concentrations. The results relevant to our assumption could be obtained by using domestic chickens as an in vivo model for the evaluation of gut microbiota-altering properties of pine needle extract as an herbal supplement. We tested the antimicrobial effects of Baltic pine (Pinus sylvestris) needle extract. Then, we used chicken (Gallus gallus) that received feed supplemented with two different concentrations of the extract for 40 days to evaluate the changes in gut microbiota using 16S rRNA gene sequencing. This preliminary study demonstrated trends toward dose-dependent desirable changes in broiler microbiome, such as a reduction in the relative abundance of Campylobacter.

Culturability, Cultivation Potential, and Element Analysis of the Culinary-Medicinal Cauliflower Mushroom Sparassis latifolia (Agaricomycetes) from Pakistan.

Sparassis Fr. is a recognized nutritionally and therapeutically significant genus of mushrooms. Its species has the potential to meet the nutritious and healthy food needs of the growing population but unfortunately due to their tough, laborious and undefined cultivation conditions, their domestication is very rare and not properly described before. In this study, first time the detailed culturabilty and cultivation potential of a wild S. latifolia species from Pakistan was evaluated. Among the different media used, maximum cultural growth was observed on pine needles extract agar (PEA) medium at 24°C. Cultured strains on PEA medium were used to spawn on wheat, sorghum and barley grains. Sorghum grains at 24°C were found as the best combination for spawn production of this mushroom. Total yield efficacy was investigated on a variety of substrates. A mixed substrate of Morus sawdust and pine sawdust at 20°C showed the optimum yield. Element analysis of wild and cultivated strain was carried out and revealed that this mushroom is enriched with macronutrients. These results showed that S. latifolia has great artificial growth potential. Its domestication can compete with commonly growing mushrooms in nutritional and pharmaceutical attributes.

Effects of Pine Needle Extracts on the Degradation of LLDPE.

Polyolefin suffers from degradation during processing and application. To prolong the service life, antioxidants are needed in the packing formula of polyolefin products. The usage of natural antioxidants could avoid potential health hazards aroused by synthetic ones. Pine needles have long lives and hardly rot, suggesting their high resistance to degradation. To provide a new candidate of natural antioxidants and add more value to pine needles, pine needle extracts (PNE) were investigated as the antioxidant of linear low-density polyethylene (LLDPE). PNE-modified LLDPE (PE-PNE) exhibited much better short-term and long-term aging resistance than pure LLDPE (PE): Oxidation induction time (OIT) of PE-PNE was 52 times higher than that of PE, and the increments of carbonyl index (CI) of PE-PNE-1st samples placed under daylight and in the dark were approximately 75% and 63% of PE under the same conditions. It could be attributed to the attractive antioxidant capacity of PNE (IC50 of DPPH radical scavenging was 115 μg/mL). In addition, the PE-PNE sample showed high processing stability and maintenance of the mechanical property during multiple extrusions: only a 0.2 g/10 min decrease in melting flow rate was found after five extrusions; the tensile strength and elongation at break were almost unchanged. All results reveal that pine needle extracts could play a role in LLDPE stabilization. Moreover, as pine needles are mainly considered a kind of waste, the present study would benefit the budget-reducing polyolefin industry.

Comparison of the influence of EDTA, nitrilotriacetic acid, diethylenetriamine pentaacetic acid, chitosan, fulvic acid and pine needle extract on the removal of multiple heavy metal by electrokinetic remediation

Comparison of the influence of EDTA, nitrilotriacetic acid, diethylenetriamine pentaacetic acid, chitosan, fulvic acid and pine needle extract on the removal of multiple heavy metal by electrokinetic remediation

Inhibitory effects of cedar pine needle extract on H9N2 avian influenza virus in vitro and in vivo

H9N2 avian influenza virus causes significant economic losses to the poultry industry, due to its wide-spread prevalence and propensity to induce secondary and mixed infections. Antigenic drift limits vaccine efficacy. New anti-viral therapies are needed to complement existing control measures. At the maximum non-cytotoxic concentration (25 mg/mL), cedar pine needle extract inhibited H9N2 avian influenza virus proliferation in vitro and in vivo. Cedar pine needle extract reduced the haemagglutinin titre, inhibited H9N2 avian influenza virus nucleocapsid protein expression, and indirectly regulate type I and II interferon expression. Interleukin-6 expression increased during the pre-infection period but decreased during the mid-to-late stages of infection. Cedar pine needle extract may inhibit the proliferation of pathogens, regulate the immune response, and reduce host tissue damage and may serve as a potential target for drug development against H9N2 avian influenza virus.

Terpenes and phenolics in alcoholic extracts of pine needles exhibit biocontrol of weeds (Melilotus albus and Asphodelus tenuifolius) and insect-pest (Plutella xylostella)

Massive use of synthetic chemicals exerts deleterious effects on human and environmental health, which calls for the development of novel alternatives to manage agricultural pests. Botanical pesticides have drawn great interest due to their non-toxic and eco-friendly nature. Therefore, under this study, leaf-needles of chir pine (Pinus roxburghii) were extracted by different solvents to obtain effective biochemical. These extracts were evaluated for biocontrol of two weed and an insect-pest species. Pine needles were soaked separately in methanol, ethanol, hot and cold water in the volume to weight ratio of 5:1 (solvent:pine needles) for 7 days. Extraction yield, and contents of organic acids, siloxanes and amides were higher with hot and cold water, while alcohols, ketones, terpenes and phenolic compounds were greater in methanolic and ethanolic extracts. These biochemicals with respective extractants increased quantitatively and progressively but not beyond 7 days. Raw extracts (with variable dilution) were employed topically against two weed species (Melilotus albus and Asphodelus tenuifolius) and an insect-pest (Plutella xylostella L.). Greater weed biocontrol efficacy through soaking of M. albus and A. tenuifolius weed seeds was exhibited by methanolic extract (100% concentration) with the highest germination inhibition (74% and 65%) followed by ethanolic extract (68% and 64%), respectively. The highest mortality (92%) of P. xylostella insects was achieved through methanolic extract bath (20% concentration) after 120 h. These results conclude that alcoholic extracts of pine needle contain more effective biochemicals (e.g., phenols and terpenes) with bio-pesticidal characteristics as compared to that from aqueous extracts. Therefore, the alcoholic extracts bear great potential to develop the bio-pesticides as novel and safe alternatives for weeds as well as insect-pest management.

Ultrasonics as a tool for development of pine-needle extract loaded bee wax edible packaging for value addition of Himalayan cheese

In the present study, Himalayan cheese, kradi was coated with beeswax loaded with pine needle extract (PNE) to increase its shelf life and nutraceutical potential. PNE was extracted via ultrasonication and incorporated into beeswax at concentrations, 2:1, 1:1, and 2:3 (grams of beeswax to mL of PNE). The dispersion of PNE in the coatings was carried out using an ultrasonic probe at a frequency of 20 kHz for 15 min and at power rating of 500 W. The coatings were characterised using scanning electron microscopy, light microscopy, dynamic light scattering (DLS), fourier transmission infrared spectroscopy. DLS revealed a hydrodynamic diameter and zeta potential of 12.11 ± 0.41 µm and −19.32 ± 0.61 mV for coating loaded with highest concentration of PNE. The bioactivities of the coating including antioxidant, antidiabetic and antibacterial assays revealed significantly higher values with the increase in PNE concentration. Shelf life and sensory evaluation study including microbiological and sensory analysis revealed inhibition of mould growth and good score of texture and appearance with the increase in concentration of PNE. The study provides a future perspective for application of beeswax loaded PNE coatings in cheese industry.

Biogenic MnO2nanoparticles derived from aCedrus deodarapine needle extract and their composites with polyaniline/activated charcoal as an electrode material for supercapacitor applications

A ternary composite of phytogenic MnO2nanoparticles with polyaniline (PA) and activated charcoal (AC) was designed and fabricated.

Pine needle extract from Cedrus deodara: Potential applications on hazardous chemicals and quality of smoked bacon and its mechanism

Effect of pine needle extract from Cedrus deodara (PNE) on the quality and safety of Chinese traditional smoked bacon was observed, and its action mechanism was further investigated. With the treatment of PNE, the quality properties of smoked bacon, including moisture content, pH and titratable acidity values, color, texture and volatile compositions, were all ameliorated. Meanwhile, the peroxide value (POV), thiobarbituric acid value (TBARS) and nitrite content of smoked bacon with PNE decreased. In addition, the content of N -nitrosodimethylamine in smoked bacon gradually decreased with the increased concentration of PNE. Moreover, the heavy polycyclic aromatic hydrocarbons (PAHs) were not detected in smoked bacon with PNE, while the types and contents of light PAHs in smoked bacon were both reduced by PNE. With the measurement of fluorescence spectroscopy and circular dichroism spectroscopy, the secondary and tertiary structures of myofibrillar protein in smoked bacon were both changed though the treatment of PNE. Furthermore, according to the analysis of molecular docking, hydrogen bond was the main molecular force between PNE and meat proteins in smoked bacon. All present results suggested that PNE had the potential value to improve the quality and safety of smoked bacon in food industry. • PNE had the ability to improve the quality of smoked bacon. • PNE could decrease the POV, TBARS and nitrite content of smoked bacon. • PNE could reduce the formation of N -nitrosodimethylamine in smoked bacon. • PNE could inhibit the formation of polycyclic aromatic hydrocarbons in smoked bacon. • PNE changed the structure and property of meat proteins by noncovalent interaction.

홍고추 또는 솔잎 초임계 이산화탄소 추출물이 들기름의 산화안정성에 미치는 영향

The objective of this study was to determine the effects of supercritical carbon dioxide (SC-CO2) extracts from red pepper (RP) or pine needles (PN) on the physicochemical properties of perilla oil (PO). Subsequently, the physicochemical properties of the perilla oil containing SC-CO2 extract at 0.1% and PO without extract were compared. Oxidative stability was measured based on viscosity, acid value (AV), peroxide value (POV), antioxidant (2,2-diphenyl-1-picrahydrazyl) activity, p-anisidine value (p-AV), and fatty acid composition. All the samples were studied under medium temperature (65°C) accelerated oxidation conditions for 24 h. The viscosities of PO with PN extract and PO without extract significantly increased with increasing storage time, but that of PO added with RP extract was not significantly different (p<0.05). The oxidative stability properties, such as AV, POV, and p-AV, indicated that PO with RP or PN extracts exhibited superior oxidative stabilities than PO without extract. The antioxidant activities of all samples was significantly decreased with increasing the oxidation time (p<0.05). No significant differences in fatty acid composition between PO with and without extract were observed (p<0.05). Thus, the SC-CO2 extracts affect the lipid oxidative stability in perilla oil and may be used as natural antioxidant for food preservation.

STUDY OF THE ELEMENTAL COMPOSITION OF WATER EXTRACTS SIBERIAN CONIFERS

In Siberia, when harvesting commercial coniferous wood and pine nuts, a large amount of waste remains in the form of needles and shells. These organic residues that require recycling may be of interest as raw materials for the production of feed additives in animal husbandry, so it is important to study their chemical composition. The study of the elemental composition of water extracts of pine nut shells and pine needles prepared under different conditions: whole and crushed shells – at an extraction temperature of 25 and 100 °С and duration of infusion 5 and 24 hours; pine needles – at an extraction temperature of 100 °С and duration of infusion 5 and 24 hours. The elemental composition was determined by inductively coupled plasma mass spectrometry. As a result of research, it was found that the most effective extraction of elements in the infusions of the studied raw materials was observed at a water temperature of 100 °С and duration of infusion 24 hours. At the same time, crushing the pine nut shell allowed to increase the degree of extraction of elements. It was found that extracts of pine nut shell from macronutrients contained the most K, Cl, Mg, P, from microelements – Zn, Mn and Fe; extracts of pine needles from macronutrients contained more K, Mg, Cl, from microelements – Mn, Zn and Fe. The highest absorption capacity was found in samples of crushed pine nut shells.

Cytotoxic activity of Pinus cembra L. needle extract: A preliminary study on HeLa cell line

The aim of this study was to investigate the cytotoxic effects of a hydromethanolic extract obtained from cembran pine needles in HeLa cell line. In this respect, the effects of needle extract on protein synthesis, viability, proliferation and cell cycle in HeLa cells were evaluated after 48 h treatment. Cembran pine needle extract dose-dependently decreased protein synthesis in HeLa cells causing 44.26% reduction in protein synthesis at 100µg/ml. At 25, 50 and 100µg/ml, it increased cell death in comparison with the control (20.99%, 21.49% and 23.63%, respectively vs. 9.83%). In addition, at 100µg/ml, cembran pine needle extract showed a remarkable antiproliferative effect whereas at 25 and 50µg/ml, it induced sub-G1 phase cells accumulation (11.68 ± 0.81% and 14.69 ± 0.56%, respectively in comparison with control, 6.03 ± 0.55%), an indicator of proapoptotic effects. Taken together, these results indicate that cembran pine needles are a source of compounds with antitumor potential which needs to be further investigated and exploited.