Datura Extracts Research Articles

Structure-based drug design-guided identification of estrogen receptor binders.

Cancer is one of the life-threatening diseases and the second leading cause of death in the world. The estrogen receptor can be considered as one of the significant drug targets for cancer. A large number of clinically used anticancer drugs were identified from phytochemicals. Multiple literatures suggested that extracts of Datura sp. significantly inhibit estrogen receptors associated with human cancer. In the present study, all reported natural products present in Datura sp. were subjected to molecular docking against estrogen receptors. The top hits were shortlisted based on binding orientation and docking score and subjected to molecular dynamics simulation to explore the conformational stability followed by binding energy calculation. The ligand [(1S,5R)-8-Methyl-8-Azabicyclo [3.2.1] Octan-3-yl] (2R)-3-Hydroxy-2-Phenylpropanoate depicts highly acceptable MD simulations outcomes and drug-likeness profile. Knowledge-based de novo design and similar ligand screening were executed using the structural information. The designed ligand DL-50 exhibited satisfactory binding, drug-likeness profile, and well-accepted ADMET profile followed by easy synthetic accessibility which further requires experimental validation.

Ethanolic Extracts of Datura innoxia Have Promising Acaricidal Activity against Rhipicephalus microplus as It Blocks the Glutathione S-Transferase Activity of the Target Tick.

Rhipicephalus microplus is a major bovine ectoparasite that negatively impacts the cattle industry. The acaricidal activity of Datura innoxia ethanolic plant extract against R. microplus, compared with trichlorfon, was examined using the adult immersion test (AIT), and larval packet test (LPT). In vitro acaricidal activity of the selected plant extract against R. microplus engorged females was evaluated at different concentrations (2.5, 5, 10, 20, and 40 mg/mL), and was the same for AIT and LPT. It was further supported by in silico molecular docking of D. innoxia's 21 phytochemicals against the R. microplus Glutathione S-transferases (RmGST) protein's three-dimensional (3D) structure predicted by the trRosetta server. The modeled 3D structure was then evaluated and confirmed with PROCHECK, ERRAT, and Verify3D online servers. To predict the binding mechanisms of these compounds, molecular docking was performed using Auto dock Vina software, and molecular dynamic (MD) simulations were used to investigate the protein atom's dynamic motion. D. innoxia has a relatively higher inhibitory effect on oviposition (from 9.81% to 45.37%) and total larval mortality (42.33% at 24 h and 93.67% at 48 h) at 40 mg/mL. Moreover, the docking results showed that the chemicals norapoatropine and 7-Hydroxyhyoscyamine have strong interactions with active site residues of the target protein, with a docking score of -7.3 and -7.0 Kcal/mol, respectively. The current work also provided a computational basis for the inhibitors of Glutathione S-transferases that were studied in this research work, and this new knowledge should aid in creating new and effective acaricidal chemicals. Furthermore, this plant extract's acaricide activity and its effect on oviposition and larval mortality were established in this work for the first time, indicating the possible use of this extract in the management of ticks.

Bio-rationale Management of Tomato Leaf Miner, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) Using Extracts of Neem (Azadirachta indica A. Juss) Jimsonweed (Datura stramonium L) and Periwinkle (Vinca rosea L.)

Recently, tomato leaf miner (TLM) Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae), is the most notorious pest of tomato worldwide causing severe reduction of yields in both protected and open fields. In Sudan, the pest was recorded to attack all tomato cultivation areas causing considerable damage affecting income of poor farmers. Various pesticides of different active ingredients are applied excessively and routinely to control T. absoluta in tomato. As known, reliance on insecticide cause adverse impacts in human health, create insect resistance as well as affect environment and natural enemies. This study is initiated to evaluate the efficacy of extracts of 3 botanicals as alternative to synthetic insecticides against T. absoluta. A randomized Complete Block Design field experiment was conducted at Takroof experimental farm of Kassala and Gash Research Station, Sudan during season (2019/2020). The experiment consisted of Neem seed oil, water extracts of Datura, Water extract of Vinca, Dancid®; synthetic insecticide as standard, and non-treated treatment as control. Each treatment was replicated thrice Data included number of infested plants/plot, number of mines/plot, percentage of infested fruits and yield (Ton/ha) were compiled, variance among treatments (ANOVA) was statistically analyzed and means were separated using Duncan Multiple Range Test (DMRT) applying SAS 9 a computer based program. The results of this study, revealed high significant difference between treatments in the number of infested plants/plot, number of active mines/plots for the three assessment times of the post spray counts. Water extract of Datura and Neem seed oil were better than Dancid® and Vinca in reducing the number of infested plants/plots and number of active mines/plots. All test products were significantly same in reduction the percentage of infestation of fruits and they were less than the control. Also high significant difference was observed between treatments on yield. Plots treated with Neem seed oil obtained the highest yield followed by WE of Datura, Dancid®, WE of Vinca with 11.2, 9.9, 9.7, 8.8 (Tons/ha) while the un-treated plots obtained the lowest yield (3.8 Tons/ ha). For their potency, cheapness, easy processing and environmental friendly characters, Neem seed oil, WE of Datura, and WE of Vinca are recommended to be used to control T. absoluta in tomato crop. Keywords: Tuta absoluta neem seed oil, Datura, periwinkle, aqueous extracts

Effect of plant extracts against stripe rust of barley caused by Puccinia striiformis f. sp. hordei

Effect of plants leaf extracts, i.e. Garlic, Oak, Neem, Dathura, Partheneium, Tulsi, Castor and Ashwagandha were studied at four different concentrations (3, 5, 10 and 15 %). Barley stripe rust disease was tested on susceptible barley cultivar RD 2035 under artificial rust inoculation conditions during two consecutive Rabi, seasons 2016-17 and 2017-18 in cage house. All concentration of eight plants leaf extracts increased per cent disease suppression and decrease the per cent disease severity, than in the control. Among these extracts garlic clove extract proved as best with highest per cent disease suppression (53.10) and significantly low mean per cent rust severity (39.78) followed by leaf extracts of neem (Azadirachta indica), tulshi (Ocimum tenuiflorum), datura (Datura stramonium), oak (Calotropics gigantean), partheneium (Parthenium hysterophorus), castor (Ricinus communis) and ashwagandha (Withania somnifera).

Effect of mycorrhizal fungi in combination with botanicals, fungicides and bio-agents in ameliorating Fusarium wilt management in chickpea (Cicer arietinum)

Fusarium wilt incited by Fusarium oxysporum f. sp. ciceris causes substantial economic losses in chickpea. Tostudy the effect of mycorrhizal inoculation in combination with different bio-agents, fungicides and botanicals onthe Fusarium wilt incidence and yield of chickpea, field trials were conducted during 2019–20 at the research field ofCCS Haryana Agricultural University, Hisar. Mycorrhizal fungi were found to be beneficial in alleviating chickpeawilt incidence and upsurging the yield components of chickpea. The mycorrhizal fungus Glomus mosseae performed better than Glomus hoi, evincing the importance of mycorrhizal species. Seed soaking provided more disease control in comparison to soil drenching of plant extracts, bio-agents and fungicides except azoxystrobin 23% SC in variety JG 62 in which mode of application of fungicide had no significant effect on disease incidence. Amongst mycorrhiza × treatment interaction, carbendazim 50% WP provided utmost disease control (>80%) in the soil pre-inoculated with G. mosseae and Glomus hoi, followed by azoxystrobin 23% SC and Trichoderma viride, while aqueous extract of datura leaves was the least effective in both chickpea varieties JG 62 and C 235.

Phytochemistry, Pharmacology, and Toxicology of Datura Species—A Review

Datura, a genus of medicinal herb from the Solanaceae family, is credited with toxic as well as medicinal properties. The different plant parts of Datura sp., mainly D. stramonium L., commonly known as Datura or Jimson Weed, exhibit potent analgesic, antiviral, anti-diarrheal, and anti-inflammatory activities, owing to the wide range of bioactive constituents. With these pharmacological activities, D. stramonium is potentially used to treat numerous human diseases, including ulcers, inflammation, wounds, rheumatism, gout, bruises and swellings, sciatica, fever, toothache, asthma, and bronchitis. The primary phytochemicals investigation on plant extract of Datura showed alkaloids, carbohydrates, cardiac glycosides, tannins, flavonoids, amino acids, and phenolic compounds. It also contains toxic tropane alkaloids, including atropine, scopolamine, and hyoscamine. Although some studies on D. stramonium have reported potential pharmacological effects, information about the toxicity remains almost uncertain. Moreover, the frequent abuse of D. stramonium for recreational purposes has led to toxic syndromes. Therefore, it becomes necessary to be aware of the toxic aspects and the potential risks accompanying its use. The present review aims to summarize the phytochemical composition and pharmacological and toxicological aspects of the plant Datura.

INVESTIGATION NON-ALKALOIDAL BIOLOGICAL ACTIVE COMPOUNDS OF DATURA INNOXIA

The purpose of the investigation is to study non-alkaloidal biological active compounds of various organs, the antimicrobial activity of plant oil and ethanolic extracts of Datura innoxia Mill. from nightshades (Solanaceae). D. innoxia grows in the natural environmental conditions of the Republic of Azerbaijan. We have studied presence of several biological active compounds – triterpenoic acids, essential oils, fatty acids, sterols and amino acids in the content of various organs of plant. Oil was obtained by using Soxhlet apparatus and investigated by GC method. After removing the oil from the seeds, triterpenoic acids - oleanolic and ursolic acids were isolated and identified by TLC. Essential oils were obtained from plant leaves by hydrodistillation and studied by GC-MS method. 20 amino acids were identified in the aerial part of the plant by the HPLC method, with amino acid analyzer L-8800 (Hitachi, Ltd.), 12 of them are non-essential, 8 are essential.
 The antimicrobial activity of plant oils of seed and ethanolic extracts of some parts (roots, stems, leaves, flowers, fruits and seeds) were studied on Staphylococcus aureus, Esherichia coli, Pseudomonas aeruginosa, Bacillus anthracoides, Klebsiella pneumoniae and Candida albicans. The significant activity of some extracts (stems, leaves, fruits and seeds) was observed against S. aureus, E. coli, C. albicans, B. anthracoides and plant oil against C. albicans.

Construing the Role of Plant Extracts, Fungicides and Bio-agents in Ameliorating Fusarium Wilt Management in Chickpea

Background: Chickpea (Cicer arietinum L.) is the most important leguminous crop around the world. Fusarium wilt incited by Fusarium oxysporum f. sp. ciceris is a major biotic constraint in chickpea production. The present investigation was undertaken to evaluate the efficacy of plant extracts, fungicides and bio-agents against Fusarium oxysporum f. sp. ciceris under in vitro and field conditions. Methods: Plant extracts and fungicides were evaluated using poison food technique while antagonistic activity of bio-agents was studied using dual culture technique under in vitro conditions. Field trials were conducted to evaluate the efficacy of different plant extracts, fungicides and bio-agents against Fusarium wilt at Experimental Area of Plant Pathology, CCS HAU, Hisar.Result: Among thirteen plant extracts evaluated in vitro, neem leaves extract was found to be the most effective in inhibiting mycelial growth of F. oxysporum f. sp. ciceris followed by datura leaves’ and garlic cloves’ extract which were statistically at par. Out of six plant extracts tested against Fusarium wilt under field conditions, seed treatment with extracts of neem and datura leaves at 10% concentration were most effective and curtailed the wilt incidence by 39.02 and 34.14% along with 7.55 and 6.83% enhanced seed yield. Among fungicides, carbendazim 50 WP was the most toxic fungicide in restricting colony diameter of the pathogen with the least EC50 and EC90 values of 1.28 and 15.24 ppm a.i. followed by azoxystrobin 23 SC with corresponding values of 1.57 and 49.16 ppm a.i., respectively. Seed treatment with carbendazim 50 WP and azoxystrobin 23 SC were superior over other treatments and provided 88.41 and 85.98% reduction in disease incidence along with 12.85 and 10.99% higher seed yield over control. Among the bio-agents tested, T. viride and T. harzianum gave the best results in suppressing the pathogen growth in vitro and in minimizing the disease incidence coupled with improvement in seed yield under field conditions. The present study has provided chemical and non-chemical measures for integrated management of chickpea wilt.

Histological and body weight effects of Datura innoxia seeds and leaves extracts in rats

The study aim to determine the chemical compounds in aqueous and methanolic extracts of Datura innoxia seeds and leaves and to evaluate their toxic effects on experimental rats. Seeds and leaves were collected from El-Obied, North Kordofan State, Sudan, in October, 2016. The aqueous and methanol extracts were carried out by using maceration method and soxhlet apparatus respectively. Sixty five male Albino Wistar rats, three months old and with an average body weight ranged 110-120 g, were randomly divided into thirteen Groups, consisting of five rats in each Group. Group 1 served as control and fed with normal rats’ food and water for thirty days. Groups 2, 6 and 10 administered aqueous seeds extract, Groups 4, 8 and 12 received methanol seeds extract, Groups 3, 7 and 11 received aqueous leaves extracts, Groups 5, 9 and 13 received methanol leaves extract, all the Groups received the same type of extract were administered 40, 60 and 80 mg/kg body weight respectively. The extracts administered to the rats intra gastrically using cathodal tube daily for thirty days. The effects of oral administration of leaves and seeds extracts to 60 healthy rats over 30 days were evaluated by histological studies and body weight changes. The analysis by gas chromatography-mass spectrometry (GC-MS) of aqueous and methanolic extracts revealed the presence of alkaloids (Scopolamine, atropine and Hyoscyamine), fatty acids, esters, amides, amino acids, ketones, coummarins, terpinoids, phenols, alcohols and hydrocarbons compounds. The histological results showed that administration of extracts caused pathologic changes in the organs studied. The treated Groups had lower (p ≤ 0.05) body weight gains than control Group. The study concluded that the toxicity of seeds and leaves (methanolic and aqueous) extracts are nearly have the same toxic effects on rats due to their same active ingredients (alkaloids) and the oral administration of the extracts was found to be safe up to 40 mg/kg.

Rosmarinus officinalis L. leaf extract improves Anxity impairment induced by tropane alkaloids extracted from Datura stramonium

The alkaloid extract of datura stramonium (rich of atropine, hyoscyamine, scopolamine) is used to evaluate its effect on the central nervous system in rats (locomotion, anxiety). The animals received the alkaloid extract of datura by intraperitoneal injection. However, after the injection a group of rats got an inhibitory treatment contain flavonoids extracted from Rosmarinus officinalis. At the dose of 50 mg/kg/body weight of the alkaloid extract, the female rats have shown a reduction of locomotor activity and an induction of an anxiogenic effect. In the other hand, the mixture of Datura stramonium and Rosmarinus officinalis showed a neuroprotective action with regard to the disorders induced by extracts of Datura stramonium.

Phytochemical Screening and Elemental Analysis of Aqueous and Methanolic Extracts of Datura innoxia Seeds and Leaves

The present study is aimed to screen the phytochemicals analysis and elements content of Datura innoxia seeds and leaves. The aqueous and methanol extracts were carried out by using the maceration method and soxhlet apparatus, respectively. Results of the study of Phytochemical screening revealed the presence of a high concentration of alkaloids, flavonoids, tannins, steroids and triterpines, and low concentration of saponin and coummarins. The elements in the leaves and seeds (K, Ca, S, Si, Cl, Fe, Al, P, Mg, Ti, Mn, Zn, Sr, Cu, V, Br, and Zr) were determined by energy-dispersive X-ray fluorescence (EDXRF) spectroscopy. K content was the highest in seeds (5.469 ± 0.021%), Ca and S the highest in leaves (2.461 ± 0.019 %, 1.254 ± 0.022 %, respectively). The elements Ti, Mn, Sr, V, Br, and Zr were detected in the leaves with range concentration 0.062-0.002 %. The elements Si, Cl, Fe, Al, P, Mg, and Zn concentration in seeds varied from 0.002 to 0.942% and in leaves varied from 0.014 to 0.346%. The concentration of these elements did not exceed the standard of dangerous toxic levels.

Management of Aedes aegypti using green silver nanoparticles and botanical extracts

The excessive use of insecticides has led to development of resistance in Aedes aegypti and negative impact on environment and non-target organisms. To overcome these problems, emphasis is being laid for alternatives, therefore, in the present study, the toxicity of eight plant extracts and their green synthesized nanoparticles were evaluated against A. aegypti. Clove extracts caused the maximum mortalities of 92% and 90% of 3rd and 4th instar larvae of A. aegypti followed by ginger causing 90% and 78% mortalities respectively. On the other hand, the minimum mortalities of these larvae were caused by neem and garlic extracts. In case of green silver nanoparticles, the maximum mortalities of 3rd and 4th instar larvae of A. aegypti were caused by clove followed by ginger while the minimum mortalities were caused by nanoparticles of datura followed by garlic. All the green silver nanoparticles caused mortalities of both the instars of the mosquito above 80% with few exceptions. Datura extracts showed the minimum LC50 values after 72 hours of application followed by neem against the 3rd and 4th instar larvae of A. aegypti. The highest LC50 value was observed in case of ginger followed by clove and eucalyptus. In case of silver nanoparticles, the minimum LC50 values after 72 hours were recorded in case of datura, neem and garlic while the values were the maximum in case of clove and ginger. The LC50 values decreased with the passage of time.

Evaluation of in vitro Antioxidant Activities of Ethanol Extracts of Datura innoxia Mill. Leaves and Seeds Harvested in Mali

Aims: The purpose of this study was to evaluate the in vitro antioxidant activities of Datura innoxia Mill. leaves and seeds harvested in Mali.
 Place and Duration of Study: Collection of plant materials were done at Kolondieba (Mali) in june 2016. Evaluation of polyphenols and flavonoids contents, determination of antioxidant activities were done at Laboratory of Plant and Food Biochemistry and Biotechnology of University of Sciences, Techniques and Technologies of Bamako (Mali) between august 2016 and March 2017.
 Methodology: The leaves and seeds were collected in Kolondieba, Mali. The phytochemical screening based on the standard methods of tube reactions has been performed with ethanol extracts. The quantitative estimation of total polyphenols was made by the Folin-Ciocalteu method and that of flavonoids by the use of aluminum trichloride. The in vitro antioxidant activities of the ethanol extracts was determined by three methods: the 1,1- diphenyl - 2- picrylhydrazyl (DPPH) test, the ferric reducing ability power (FRAP) test and the Phosphomolybdate test (PPM).
 Results: The phytochemical screening revealed that leaves and seeds of this plant contains alkaloids, polyphenols, flavonoids, coumarins, tannins, triterpenes and saponin. Phenolic contents of ethanol extracts are 30.97 ± 0.33 mg equivalent gallic acid / g in leaves and 14.02 ± 0.15 mg equivalent gallic acid / g in seeds; those of flavonoids are 15.13 ± 0.2 mg equivalent of quercetin / g in the leaves and 4.93 ± 0.41 mg equivalent of quercetin / g in the seeds. The three tests showed that the leaves have a higher level of antiradical activity in vitro than seeds.
 Conclusion: The results of this work showed that Datura innoxia Mill. has a good antioxidant activity which would justify its use as a potential source of natural antioxidants.

A novel process for scopolamine separation from Hindu Datura extracts by liquid–liquid extraction, macroporous resins, and crystallization

ABSTRACTAn efficient separation method of scopolamine from Hindu Datura extracts was successfully developed by combining liquid–liquid extraction, macroporous resins, and crystallization. First, the extraction solution was successively performed with liquid–liquid extraction by acid water and carbon tetrachloride. Secondly, D151 resin was selected from six tested resins, and the separation parameters were optimized. Finally, scopolamine hydrobromide was obtained after crystallization at −20°C overnight. Through one run of above combined treatments, the content of scopolamine increased from 3.02% (w/w) to 99.12% (w/w). In addition, the products were assessed by high-performance liquid chromatography (HPLC).

Lethal Effects of Toxic Plants (Neem, Datura, Acanthus) on Artemia Species

Effects of the water-soluble extracts (dry leaf powder of Azadirachta indica, Datura stramonium and Acanthus ilicifolius) were studied on the behavioral pattern and mortality of Artemia species. The extracts of all the plants brought significant change in the behavioral pattern of Artemia. The Artemia become agitated just offer exposure to the extract and started to move upwards and downwards vigorously. The Artemia exposed to the various concentration of water-soluble plant extracts were unable to maintain dorso-ventral position and dropped to the bottom finally. The mortality rates recorded after 24 h exposure to the extract Azadirachta indica were 30% at 1500 mg/L and 70% at 3000 mg/L. Similarly, after 24 h exposure to the extracts of Datura stremonium, the mortality rates were 40% at 2000 mg/L and 75% at 3500 mg/L. Again, after 24 h has exposure to the extracts of Acanthus ilicifolius, the mortality rates were 35% at 2500 mg/L and rose to 80% at 4000 mg/L. The 24 h LC50 values of the water-soluble extracts of Neem, Datura and Acanthus were 2331.930, 2357.948 and 2922.957. Artemia was more sensitive to the water-soluble extract of Neem than Datura and Acanthus.

Study of the effect of some alcoholic plant extracts in the control of adult domestic flies. Musca domestica

In this study, the toxic effect of the plant extracts of Mentha Piperita, Datora innoxia, Olea europea, and Dichlorvos (DDVP) were investigated in the domestic flies of Musca domestica. The domestic flies were treated with plant extracts and diclorophosphate with a fine spray method at 30 ± 2 ° C and 70 ± 5% relative humidity. Results were observed 24 to 48 hours of treatment respectively.
 The results obtained from the present study showed that the vegetal extracts of Datura and Olives were given the highest corrected mortality rates (75.6 and 74.2) for the extracts of Datura and Olives respectively, and through these arithmetic averages, The insect also did not differ statistically but differed in the rates of killing from the ethanol extract of mint leaves and the Dichlorvos (DDVP) used in the experiment.

Control of root-knot disease of brinjal (Solanum melongena L.) by the application of leaf extracts of certain medicinal plants

Microscopic plant parasitic nematodes are round worms that feed on plants and damage them. The most common and serious amongst the nematodes is the root-knot nematode found throughout the country with a very wide host range of cultivated crops. Brinjal (Solanum melongena) crop yield is considerably reduced by root-knot nematodes particularly Meloidogyne incognita. Root-galls of brinjal caused by Meloidogyne incognita can be controlled effectively by using the bio-agents and botanicals which in turn increases the yield and plant growth. Present study explored the effect of leaf extracts of certain plants on the root-knot development, plant growth and nematode multiplication in pot experiments. Extract treated and nematode inoculated plants showed improvement on growth over untreated inoculated plants and reduced the disease development. Five (viz., margosa, marigold, datura, hemp and tobacco) leaf extracts, irrespective of concentration, apparently induced tolerance in inoculated plants as evidenced by better plant growth in comparison to control and suppressed the multiplication of nematode which resulted in low root-knot index. Margosa (Azadirachta indica) leaf extract suppressed the nematode multiplication, reduced root galling and improved the plant growth of inoculated plants. Margosa and marigold (Tagetes erecta) leaf extracts were found more effective in controlling the root-knot development than the leaf extracts of hemp (Cannabis sativa), datura (Datura metel) and tobacco (Nicotiana tabacum). Higher concentrations (20 ml/plant) of leaf extracts were found to be more effective in controlling the root knot development. Based on our observations, it may be concluded that margosa or marigold extract treatment of brinjal seedlings prior to transplantation can avoid severe damage by suppressing the nematode multiplication and inducing the tolerance in treated inoculated plants.

Study of the efficacy of Lambdacyhalothrin 10% wp and the Fruits extracts of Datura innoxia plant in the fourth larval instar and adults of sand fly Phlebotomus papatasi (Diptera: Psychodidae)

The results of the present study, which aimed to demonstrate the efficacy of Lambdacyhalothrin 10% and alcoholic extracts of Datura innoxia fruits in the control of fourth larval instar and adults of the phlebotomus papatasi , indicated that the highest mortality percentage of the larval instar was 100% in concentration 3 μL / ml, and the lowest mortality percentage of 33.3% at the lowest concentration of 0.05 μL / ml. The results of adult treatment were the highest mortality percentage of 93.33% at the concentration of 3 μL / ml and the lowest mortality percentage were 20% at concentration of 0.05 μL / ml after 24 Hour of treatment.
 The results of the treatment of the fourth larval instar with the Datura innoxia fruits extracts showed that the highest mortality percentage was 100% for the concentration of 2000 ppm and the lowest of 13.3% at 75 ppm. The highest mortality percentage of adults insects was 100% when treated with the most effective concentrations (1600, 2000ppm). Thus, the results are shown to be affected by the control method used and the interaction of the insect larval instar to this method. In the end, there was a convergence of the effect of the insecticide and extract in the larval instar and adults insect.

Assessment of In vivo Antiviral Potential of Datura metel Linn. Extracts against Rabies Virus.

Objective:The soxhlet, cold, and ayurvedic extracts of Datura metel Linn. were evaluated for in vivo antirabies activity.Materials and Methods:Soxhlet and cold extraction method were used to extract Datura (fruit and seed) extracts, and ayurvedic extraction of Datura was prepared. In vivo toxicity assay was performed as per the OECD 420. LD50 dose was calculated by Reed and Muench method. The in vivo antirabies activity was screened in Swiss albino mice with the virus challenge dose of 10 LD50 (intracerebrally) in both preexposure (PE) and postexposure treatment with oral administration of Datura extracts in Swiss albino mice and observed for 21 days. The virus load in the mice brain was evaluated by TCID50 titration method.Results:Datura (ayurvedic preparation) was found to be nontoxic up to 2000 mg/kg in Swiss albino mice, i.e., 60 mg/30 g of mice, when administered (0.5 ml) orally and observed till 21 days. Up to 20% survival rate on the test group (PE of Datura extracts) up to 14 days postinfection as compared to the virus control group (10 LD50) was observed. No survival rate was observed in the postexposure group of Datura extract; however, the survival time was increased by 4 days as compared to the virus control group. Viral load of the infected mice brain sample was estimated in vero cell line, and 3 log reduction in the virus titer was observed in text group as compared to the virus control, suggesting that Datura extract has an in vivo antirabies activity.Conclusion:To the best of our knowledge, this is the first study of in vivo antiviral activity of an ayurvedic preparation of D. metel Linn. against rabies virus. Datura extracts have a potential in vivo antirabies activity.SUMMARY In the present study, Datura metel Linn. (ayurvedic preparation) extract exhibited survival (20%) in the preexposure (PE) of the virus and the survival time was increased in the postexposure treatment where the disease was established. The mortality was observed, and the viral load was determined by titration method. Abbreviation Used: TCID50: tissue culture infectious dose 50; LD50: lethal dose 50; RV CVS: Rabies virus challenge virus standard; PE: Pre exposure; IC: intracerebral; PI: post infection; FITC: Fluorescein isothiocyanate

Field Evaluation of Bio and Synthetic Pesticides Against Jassid (Amrasca Devastans Dist.) on Okra

Bio and synthetic pesticides were evaluated against jassid (Amrasca devastans Dist.) on okra during 2014 and 2015 at Murredke, Punjab.The treatments included neem oil, extracts of neem, tobacco, akk, tooh/tumma, datura, garlic, eucalyptus and ginger; while synthetic pesticides include Tracer 45SC, Runner 240 SC, Radiant 120 SC, Coragen 20 SC and Belt 480 SC. All the bio and synthetic pesticides were effective and showed highest efficacy 7 days after spray (DAS) as jassid population started increasing 14 DAS during both the years. During 2014, among bio and synthetic pesticides, tobacco extract and Runner produced maximum jassid mortality of 64.33and 87.30% after 1st spray, while 63.89 and 90.86% mortality 7 DAS, respectively. During 2015, neem extract among biopesticides and Runner 240 SC among synthetic pesticides resulted in highest insect mortality of 65.37 and 92.84% after 7 days of 1st spray, while 67.33 and 88.67% mortality after 7 days of 2nd spray, respectively.