Cymbopogon Citratus Extract Research Articles

Phytochemical profiling and anticancer potential of Cymbopogon citratus extract

Objective: To evaluate the anticancer potential of Cymbopogon citratus extract. Methods: GC-MS analysis was used to identify phytocomponents in the methanolic extract of Cymbopogon citratus. A fractionation method was employed to isolate and assess the bioactivity of different fractions and their cytotoxic activities against cancer cell lines HCT116, LoVo, Caco-2, and HT-29 were investigated. A dual staining method with acridine orange and ethidium bromide was used to assess the effect of the extract on cell apoptosis. Additionally, the expression levels of Bax and TP53 were quantified using realtime PCR in Caco-2 cells treated with the ethyl acetate fraction of Cymbopogon citratus extract. A protein array was employed to profile key pro- and anti-apoptotic proteins in Caco-2 cells. Moreover, molecular docking studies were conducted to investigate the interactions between key compounds of Cymbopogon citratus extract and specific apoptosis-related protein domains (PDB IDs: 7wql and 4bkx). Results: A significant growth inhibition was observed in Caco-2 cells treated with Cymbopogon citratus extract. Among the seven fractions of the plant extract, the ethyl acetate fraction showed the highest cytotoxicity against Caco-2 cells with an IC50 value of (6.16 ± 0.01) μg/mL. The immunofluorescence assay showed that the ethyl acetate fraction could induce apoptosis of Caco-2 cells. Moreover, the fraction upregulated the gene expressions of Bax and TP53 in a dose-dependent manner. The docking analysis demonstrated the interaction of five compounds isolated from the ethyl acetate fraction with key proteins in Caco-2 cells, indicating their anticancer properties. Conclusions: Cymbopogon citratus extract shows anticancer activity against Caco-2 cells by inducing apoptosis. It may be a promising candidate for the treatment of colon cancer, which needs further investigation.

Characterization and Management of Post-harvest Fungal Diseases in Banana (Musa paradisiaca)

Banana (Musa spp.) is a vital crop globally, contributing significantly to food security and income generation, particularly in tropical regions like India. However, post-harvest fungal diseases pose a considerable threat to banana production, affecting both fruit quality and marketability. The current study investigated the isolation, characterization, and management of post-harvest fungal diseases in banana (Musa paradisiaca). Infected banana fruits were collected from the Dharashiv fruit market (Maharashtra, India), and pathogenic fungi were isolated and identified. Fusarium napiforme, Talaromyces atroroseus, Cladosporium cladosporioides, Fusarium sp., and Fusarium equiseti were the primary pathogens identified. DNA extraction and sequencing were employed for accurate identification, and sequences were submitted to GenBank. The antifungal activity of essential oils and plant extracts was evaluated using the Poisoned Food Technique. Essential oils from Syzygium aromaticum, Mentha piperita, and Punica granatum showed significant inhibition (P<0.05) of fungal growth, with clove and peppermint oils achieving 100% inhibition at higher concentrations. Plant extracts of Ocimum sanctum, Eucalyptus globulus, Mentha piperita, Zingiber officinale, Curcuma longa, Azadirachta indica, Piper betel, and Cymbopogon citratus were also tested, revealing notable efficacy, particularly with neem and peppermint extracts. The study that the efficacy of essential oils was more compared to aqueous plant extracts. The results suggested sustainable strategies for managing post-harvest fungal diseases in bananas and explained the importance of conducting field trials to validate laboratory results.

Phytochemical Screening and Evaluation of Syzygium jambolanum seeds for In- vitro Antimicrobial Potential against Herbal Drugs Contaminants

The disc diffusion method, MIC and MBC were used to test the antibacterial activity of methanol, aqueous, ethyl acetate extract and essential oils of Cymbopogon citratus leaves, and Syzygium jambolanum seeds against Escherichia coli and Bacillus cereus, which were isolated from herbal medicines. Methanolic extract of Syzygium jambolanum was highly active against Bacillus cereus and E. coli. Chemical analysis was done by GC- MS. All the plant extracts showed different kinds of phytochemicals like terpenoids, flavonoids and steroids etc.

Unveiling the chemical profile, synergistic antibacterial and hemolytic effects of Cymbopogon citratus and Tachyspermum ammi leaves

Current study was performed to investigate the antioxidant, synergistic antibacterial and hemolytic potential of aqueous (AC & AT) and ethanolic (EC & ET) extracts of Cymbopogon citratus and Trachyspermum ammi leaves. The investigated extracts were characterized by FT-IR, HPLC, GCMS and ICP analyses. FT-IR data verified the existence of N–H, O–H, C–O, C=O, S=O, C–N, C=N, C–H(aromatic), Si–C and Si–O moieties in extracts. The highest values of TPC (97.09 mg/100g GAE) and TFC (75.76 mg/100gCE) were shown by the ethanolic extract of T. ammi whereas the aqueous extract of C. citratus showed the lowest quantities of TPC (4.07 mg/100g GAE) and TFC (2.43 mg/100g CE). GC-MS analysis showed the existence of 45, 30, 24 and 8 compounds in AC, EC, ET and AT extracts, respectively. The highest concentrations in AC, EC, ET and AT were observed for 3-hydroxy-3-methylbutanoic acid (11.99%), lupeol (23.26%), phytol (15.53%) and phenylmethylsulfonyl fluoride (58.49%). ICP analysis determined the concentration of 26 metals in extracts. AC possessed the highest concentrations of Na, Ca, Mg and K and may find uses as a nutritional/medicinal supplement of these metals. The ethanolic extract of each plant was richer in antioxidant contents; the ethanolic extract of T. ammi being the richest with 69.07% radical scavenging activity relative to BHT (83.54%). Mixtures of plant extracts exhibited higher biofilm inhibitions than their ingredients or ciprofloxacin against S. aureus and E. coli. Hemolytic activities of all the investigated leaf extracts were found in the safe range.

Effects of aqueous extract of Cymbopogon citratus leaves on physical endurance and visceral fat mass in wistar albino rats

Effects of aqueous extract of Cymbopogon citratus leaves on physical endurance and visceral fat mass in wistar albino rats

Effect of Ethanol Extract of Cymbopogon citratus on Blood Sugar and Haematological Indices in Alloxan-Induced Diabetic Wistar Rats

Effect of Ethanol Extract of Cymbopogon citratus on Blood Sugar and Haematological Indices in Alloxan-Induced Diabetic Wistar Rats

Phytonutrients, Acute Toxicity and the Effect of Dichloromethane-Methanol Extract of Cymbopogon citratus (DC. ex Nees) Stapf (Poaceae) on Selected Haematological Parameters in Normal Healthy Albino Rats.

This study aimed to evaluate the effect of the Dichloromethane-Methanol (DCM-MeOH) extract of Cymbopogon citratus on selected haematological parameters and to identify the phytochemical components present in the extract. The (DCM-MeOH) extract of the plant was obtained by 72 h cold maceration and was subjected to haematological screening. Four groups of rats were used; group 1 served as normal control which received distilled water, groups 2, 3, and 4 received daily administration of (DCM-MeOH) extract of C. citratus (100, 200 and 400 mg/kg) for 21 days per oral. Haematological analyses were performed weekly for the red blood cell count (RBC), white blood cell count (WBC), packed cell volume (PCV), and haemoglobin concentration (HB) with an absolute count of differential leucocyte were determined. The acute toxicity study on the extract did not produce any lethality up to 5000 mg/kg in mice. The result of the study showed a significant (P < 0.05) effect to varying degrees in the weight and haemoglobin while the RBC, PCV, differential leucocyte count (DLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) did not have significant (P > 0.05) effect as compared with the normal control group and baseline values. The qualitative phytochemical screening showed the presence of flavonoids, terpenoids, alkaloids, steroids and reducing sugars while protein, carbohydrate, fat and oil, glycosides and saponins were absent in the extract. However, quantitative phytochemical studies showed that the extract contained alkaloids (465.83 mg/100g), flavonoids (119.00 mg/100g), tannins (16.25 mg/100g), terpenoids (203.36), total phenols (1897.58). The findings from this study showed that DCM-MeOH extract of C. citratus does not have any significant effect on most haematological parameters in normal healthy albino rats.

In Vitro and in Vivo Assays of Selected Plant Extracts Against Fruit Rot Fungi

Post-harvest losses of crops in Malaysia is estimated at around 20%. Fungal infection is one of the primary causes of post-harvest loss in fruits. The common use of synthetic fungicide to combat fruit rot fungi poses negative impacts on human health and the environment. The objective of this study was to determine the efficacy of selected plant species as a safer alternative for post-harvest treatment. Ethanolic leaf extracts of Piper sarmentosum, Psidium guajava, and Cymbopogon citratus were tested against Fusarium oxysporum from tomato rot, Fusarium proliferatum from banana rot, and Colletotrichum gloeosporioides from mango rot. In vitro analysis was conducted using poisoned food bioassay and the percentage inhibitions of Fusarium oxysporum growth were 93.50% by Piper sarmentosumextract, 46.68% by Psidium guajavaextract, and 40.48% by Cymbopogon citratus extract. A test on a different fungus, Fusarium proliferatum, showed that Piper sarmentosum, Psidium guajava, and Cymbopogon citratusextracts displayed percentage inhibitions of 88.78%, 36.66%, and 22.44%, respectively. In vitro test on Colletotrichum gloeosporioides showed that Piper sarmentosum extract inhibited the fungal growth by 96.23%, while Cymbopogon citratus and Psidium guajava extract inhibited the fungal growth by 52.62% and 52.22%, respectively. The mechanism of action of these extracts appeared to be fungistatic. Piper sarmentosum was selected for further analysis by in vivo assay as it displayed the most significant anti-fungal action. The study was conducted using Piper sarmentosumleaf extract of various concentrations (25, 50, 50, and 100 mg/mL). The leaf extract displayed dose-dependent activity, with the highest reduction in disease severity observed at concentration of 100 mg/mL. The findings showed the potential use of Piper sarmentosum, Psidium guajava, and Cymbopogon citratus leaf extract as anti-fungal agent for post-harvest treatment to minimize fruit loss. Piper sarmentosum leaf extract showed comparable inhibitory action to commercial fungicide carbendazim.

Formulation and evaluation of bio-mosquito repellent air freshener gel from oil extract of cymbopogon citratus (DC.) Stapf (lemon grass) plant.

Synthetic based mosquito repellents pose varying degrees of threats to public health in most developing nations. The developments has necessitated the need for environmentally friendly alternatives, which will have non-toxic, and biodegradable attributes. In this work, a mosquito repellent gel formulation was prepared from the essential oils of Cymbopogon citratus (DC.) Stapf (lemon grass) extracted using a steam distillation system. The yields of the extracted oils were determined and calculated under varying conditions. The oil extract was then purified in the micro column parked with silica gel and characterized using Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS analysis of the essential oil revealed the presence of β-pinene, isoneral, nerolidol and squalene chemical constituents. The optimized gels were evaluated for their appearance, pH, spreadability, macroscopic stability test, swelling index, and hedonic evaluation. The mosquito repellent potential was simultaneously compared with the positive control ‘Odomos’, a commercial gel sold in the open market within Jos, Plateau State, Nigeria by varying the repellency time. The outcome of the evaluations confirmed that the formulated gels were stable with normal skin pH. The hedonic test indicated that formulation F5 is the most potent gel with a C. citratus oil concentration of 5% in the formulation. This gel formulation promises to be an environmentally sustainable alternative for repelling mosquitoes.

Biological Activity of Biosynthesized Silver Nanoaggregates Prepared from the Aqueous Extract of Cymbopogon citratus against Candida spp.

Cymbopogon citratus is commonly used in folk medicine for the treatment of nervous and gastrointestinal disturbances and other medical issues because of its potent antioxidant capacity. The current study evaluated the anti-candida effects of silver nanoparticles (AgNPs) synthesized from an aqueous extract of C. citratus against different Candida spp. The aqueous extract was prepared from the fresh leaves of C. citratus. The silver nanoparticles (AgNPs) were prepared and validated by UV spectroscopy, Fourier-transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and zeta size analysis. C. albicans, C. krusei, C. parapsilosis, C. tropicalis, C. famata, C. rhodotorula, and C. glabrata were used in the antifungal assay. Microscopical imaging were used to investigate the different morphological changes induced by treatment. FTIR spectrum confirmed the existence of various functional groups of biomolecules capping the nanoparticles. The average particle size of synthesized AgNPs was 100.6 nm by zeta-sizer and 0.012 to 0.059 mm by TEM. In the antifungal assay, AgNPs aggregates induced significant inhibition of the growth of all species (p < 0.05) compared to the control and the biofilm maturation in C. famata and C. albicans. These considerable antifungal activities might lead to the development of appropriate alternative remedy for the treatment of fungal infections.

Green Synthesis of Zinc Oxide Nanoparticles Using Cymbopogon citratus Extract and Its Antibacterial Activity.

Excessive use of antimicrobial medications including antibiotics has led to the emerging menace of antimicrobial resistance, which, as per the World Health Organization (WHO), is among the top ten public health threats facing humanity, globally. This necessitates that innovative technologies be sought that can aid in the elimination of pathogens and hamper the spread of infections. Zinc oxide (ZnO) has multifunctionality owing to its extraordinary physico-chemical properties and functionality in a range of applications. In this research, ZnO nanoparticles (NPs) were synthesized from zinc nitrate hexahydrate, by a green synthesis approach using Cymbopogon citratus extract followed by characterization of the NPs. The obtained X-ray diffraction peaks of ZnO NPs matched with the standard JCPDS card (no. 89-510). The particles had a size of 20-24 nm, a wurtzite structure with a high crystallinity, and hexagonal rod-like shape. UV-Vis spectroscopy revealed absorption peaks between 369 and 374 nm of ZnO NPs synthesized from C. citratus extract confirming the formation of ZnO. Fourier transform infrared confirmed the ZnO NPs as strong absorption bands were observed in the range of 381-403 cm-1 corresponding to Zn-O bond stretching. Negative values of the highest occupied molecular orbital-lowest unoccupied molecular orbital for ZnO NPs indicated the good potential to form a stable ligand-protein complex. Docking results indicated favorable binding interaction between ZnO and DNA gyrase subunit b with a binding energy of -2.93 kcal/mol. ZnO NPs at various concentrations inhibited the growth of Escherichia coli and Staphylococcus aureus. Minimum inhibitory concentration values of ZnO NPs against E. coli and S. aureus were found to be 92.07 ± 0.13 and 88.13 ± 0.35 μg/mL, respectively, at a concentration of 2 mg/mL. AO/EB staining and fluorescence microscopy revealed the ability of ZnO NPs to kill E. coli and S. aureus cells. Through the findings of this study, it has been shown that C. citratus extract can be used in a green synthesis approach to generate ZnO NPs, which can be employed as alternatives to antibiotics and a tool to eliminate drug-resistant microbes in the future.

Effect of Plant Extract on Pathogens Isolated from Water Source

This study was designed to evaluate the effect of Aloe barbadensis Miller (Aloe vera) and Cymbopogon citratus (Lemongrass) extracts on pathogenic bacteria isolated from surface and ground water samples in Port Harcourt Metropolis. Samples of Aloe vera and Lemon grass were collected, pre-treated and extracted using n-hexane and ethanol respectively. The plant extracts were qualitatively and quantitatively assessed for their phytochemical properties using standard methods. Surface and ground water samples were collected and characterized for their physico-chemical and microbiological properties using standard methods. The pathogenic bacteria isolated from the water samples were subjected to antibacterial susceptibility tests using selected commercial antibiotics and the plant extracts respectively. Result revealed the presence of eight phytochemicals namely alkaloid, flavonoid, saponnin, tannin, glycoside, steroid, phenol and terpene in both hexane and ethanol extracts of the Lemongrass and Aloe vera with varying degrees. Four bacterial pathogens namely Streptococcus spp., Shigella spp., Pseudomonas spp. and Staphylococcus spp. were identified in the water samples. All of them were susceptible to both hexane and ethanol extracts of the Lemongrass and Aloe vera plants with zones of inhibition ranging from 10.2 mm to 14.5 mm and 16.7 mm to 20.9 mm and 4.2 mm to 9.5 mm and 6.7 mm to 11.2 mm, respectively. Commercial antibiotics such as Ciproflox, Streptomycin, Chloramphenicol and Cuntamycin were active against all the bacteria pathogens, with zones of inhibition ranging from 15.7 mm to 21.2 mm, 13.2 mm to 21.3 mm, 3.1 mm to 14.5 mm and 3.3 mm to 14.7 mm, respectively. The antibacterial action of ethanol extract of the Lemongrass on all four pathogens was comparable to that of commercial antibiotics such as Ciproflox, Streptomycin and Ofloxacin which also demonstrated higher antibacterial action on the pathogens. This study has revealed the usefulness of Aloe vera and Lemongrass plants in phyto-antibiotics and phyto-medicine.

Plant Extracts Induced Resistance to Fusarium oxysporum f.sp. lycopersici in Tomato

Tomato (Lycopersicon esculentum Mill.) is the most consumed vegetable in the world after potato. In Cameroon, the plant is cultivated in almost all agroecological areas, however, yields remain low due to attacks by various pathogens and insects. Among the pathogens, Fusarium oxysporum f.sp. lycopersici (FOL) is a phytopathogenic fungus responsible for Fusarium wilt, a disease responsible for enormous economic losses. To contribute to the control of this microbial pathogen, the stimulatory effect of the tomato defence system of extracts of some plants in the tomato/FOL interaction was evaluated. Tomato plants were treated with the aqueous extracts (AE) of Callistemon citrinus (C. citrinus), Cymbopogon citratus (C. citratus), and Oxalis barrelieri (O. barrelieri ). After 4 days of spraying with the extracts, the plants were inoculated with a virulent strain of Fusarium oxysporum f.sp. lycopersici (FOL) under controlled conditions. Tomato roots were used to determine the contents of hydrogen peroxide (H2O2), phenols and malondialdehyde (MDA). The activities of the main antioxidant enzymes were also evaluated in tomato roots: catalase (CAT), phenylalanine ammonia lyase (PAL), peroxidase (POX) and superoxide dismutase (SOD). The results showed that treatment of tomato plants with plant extracts and their infection with FOL induced an increase in the contents of H2O2, phenols and MDA in tomato roots; an increase in PAL, POX, SOD activities and a reduction in CAT activity. Our results suggest that the increase and reduction of enzymatic activities, and the increase in the synthesis of some metabolites could mitigate the oxidative damage that takes place during the expansion of the pathogen. Aqueous extracts of C. citrinus, C. citratus and O. barrelieri at 10% (W/V) could be used as natural products to stimulate the tomato defence system against FOL. These results could contribute to the development of natural products to induce tomato resistance against FOL, thus improving productivity, quantity and quality of production.

Phytochemical screening and effects of aqueous extracts of Cymbopogon citratus(DC) Staptand Origanum vulgare (LINN) on the biochemical and haematological profileof Wistar strain albino rats

Medicinal plants have played a major role in the field of medicine as alternative therapy to orthodox drugs. This present study aimed to evaluate the acute toxicity of Cymbopogon citratus and Origanum vulgare aqueous extracts on albino rats.The qualitative phytochemical analyses of the was done using standard procedures. The LD50 was determined using modified Lorke’s method, while the biochemical and haematological analysis were done by automated methods. Results from phytochemical analysis reveals the presence of Alkaloids, Flavanoids, Phenols and tannins, Steroids and sterols, Carbohydrates, Saponins, Protein and amino acid and Anthraquininein the two extracts. Furthermore, the electrolytes(urea and creatinine) were within the normal range in rats of the control and the orally administered aqueous extracts of Cymbopogon citratus and Origanum vulgare (100 mg/kg, 200 mg/kg and 400 mg/kg). Total protein, alkaline phosphate, total bilirubin, conjugate bilirubin, alanine aminotransferase, albumin and Aspartate aminotransferase were also within the normal range in both control and groups treated with 100mg/kg and 200mg/kg and 400mg/kg. White blood celland platelet followed same trend of falling within the normal range while Packed cell volume, Haemoglobin values were higher than the normal range across the control and test groups. Aqueous crude extracts ofC. Citratusand O. vulgarecontain primary and secondary metabolites thus with potential medicinal properties as anti-inflammatory, anti- malaria, anti- viral, anti- bacteria and anti-fungal agents. Orally administered aqueous crude extracts C. citratus and O. vulgare (100mg/kg, 200mg/kg and 400mg/kg)have no toxicological/pathological effect on wistar strain albino rats and as such, indigenous plants safe for consumption.

Synergistic Antibacterial Screening of Cymbopogon citratus and Azadirachta indica: Phytochemical Profiling and Antioxidant and Hemolytic Activities.

Current studies were performed to investigate the phytochemistry, synergistic antibacterial, antioxidant, and hemolytic activities of ethanolic and aqueous extracts of Azadirachta indica (EA and WA) and Cymbopogon citratus (EC and WC) leaves. Fourier transform infrared data verified the existence of alcoholic, carboxylic, aldehydic, phenyl, and bromo moieties in plant leaves. The ethanolic extracts (EA and EC) were significantly richer in phenolics and flavonoids as compared to the aqueous extracts (WA and WC). The ethanolic extract of C. citratus (EC) contained higher concentrations of caffeic acid (1.432 mg/g), synapic acid (6.743 mg/g), and benzoic acid (7.431 mg/g) as compared to all other extracts, whereas chlorogenic acid (0.311 mg/g) was present only in the aqueous extract of A. indica (WA). Food preservative properties of C. citratus can be due to the presence of benzoic acid (7.431 mg/g). -Gas chromatography-mass spectrometry analysis demonstrated the presence of 36 and 23 compounds in A. indica and C. citratus leaves, respectively. Inductively coupled plasma analysis was used to determine the concentration of 26 metals (Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, Pb, Sb, Se, Si, Sn, Sr, V, Zn, Zr, Ti); the metal concentrations were higher in aqueous extracts as compared to the ethanolic extracts. The extracts were generally richer in calcium (3000-7858 ppm), potassium (13662-53,750 ppm), and sodium (3181-8445 ppm) and hence can be used in food supplements as a source of these metals. Antioxidant potential (DDPH method) of C. citratus ethanolic extract was the highest (74.50 ± 0.66%), whereas it was the lowest (32.22 ± 0.28%) for the aqueous extract of A. indica. Synergistic inhibition of bacteria (Staphylococcus aureus and Escherichia coli) was observed when the aqueous extracts of both the plants were mixed together in certain ratios (v/v). The highest antibacterial potential was exhibited by the pure extract of C. citratus, which was even higher than that of the standard drug (ciprofloxacin). The plant extracts and their mixtures were more active against S. aureus as compared to E. coli. No toxic hemolytic effects were observed for the investigated extracts indicating their safe medicinal uses for human beings.

Free and bound phenols from Cymbopogon citratus mitigated hepatocellular injury in streptozotocin-induced type 1 diabetic male rats via decrease in oxidative stress, inflammation, and other risk markers

Background/AimDiabetes mellitus is a debilitating disease still prevailing in the developing world. This study aimed to examine the protective potentials of Cymbopogon citratus extracts (crude (CCC), free (CFP), and bound phenol (CBP) fractions), in streptozotocin (STZ)-induced type 1 diabetic male rats. MethodsForty-five (45) animals of about 3–4 months were used for the study. About forty animals were induced with STZ. After induction and confirmation of diabetes, animals were grouped (five animals per group) into nine groups; group 1 (Control), group 2 received 50 mg/kg body weight (bw) (STZ). Groups 3, 4 and 5 were healthy animals and received C. citratus extracts (200 mg/kg bw CCC, 100 mg/kg bw CFP, and 100 mg/kg bw CBP) respectively; while groups 6 – 9 were diabetic group (induced with STZ) and later treated with C. citratus extracts (200 mg/kg bw CCC, 100 mg/kg bw CFP, 100 mg/kg bw CBP, and metformin (200 mg/kg bw) respectively for 14 days. Then, the animals were euthanized, blood/serum was collected, liver samples were collected and divided into two; for biochemical and histopathological studies. Liver biomarkers (ALT, AST, ALP, GGT), fructose-1,6-bisphosphatase, hexokinase, antioxidant enzymes (SOD, CAT, and GST) activities, albumin, bilirubin, hepatic glucose, GSH, MDA, pro-and anti-inflammatory cytokines concentrations, were estimated using standard protocols. ResultsOur results revealed that C. citratus extracts counteracted the deleterious effects of type 1 diabetes induction with STZ by optimizing the liver biomarkers, antioxidants enzymes, MDA and GSH levels, reducing the levels of pro-inflammatory cytokines and increasing anti-inflammatory. ConclusionOur results correlated with previous researches; the free phenols fraction revealed brilliant results above others. These suggested that C. citratus could serve as an alternative therapy in ameliorating liver complications linked to oxidative damage and inflammation in STZ-induced type-1 diabetes.

Neuroprotective Effect of Ethanolic Leaf Extracts of Vernonia Amygdalina and Cymbopogon Citratus on Diabetes Induced Cognitive Impairments in Mice

Evaluation of learning and memory enhancing efficacy of ethanolic leaf extracts of vernonia amygdalina and cymbopogon citratus was studied in alloxaninduced diabetic mice to scientifically validate its usefulness traditionally. Upon induction and confirmation of diabetes using alloxan monohydrate, forty mice were randomly divided into 8 groups of 5 mice each and treated with low and high doses of the extracts (400 and 800mg/kg). Metformin, a standard antidiabetic drug was used as a reference agent and given to group 8. Results revealed that diabetes induced memory impairment in neurobehavioural assessment and histomorphological examination of the hippocampus. However, administration of ethanolic leaf extracts of vernonia amygdalina and cymbopogon citratus reveals its neuroprotective potential by ameliorating the histological alterations in the hippocampus (CA1 region) relative to the control and attenuating the learning and memory impairments when compared with the control. Findings from this study suggests the ethanolic leaf extracts of vernonia amygdalina and cymbopogon citratus are potentially efficacious in attenuating diabetes induced memory deficit and alterations in the hippocampus of mice and could be utilized for the treatment and management of diabetes and dementia.

Evaluation of the potentials of Jatropha curcas seed oil and in combination with leaf extracts of Cymbopogon citratus, Ocimum basilicum, and Eucalyptus globulus as wood preservatives against Macrotermes bellicosus termites

The preservation of non-durable woods such as Ayous through the use of environment friendly products is nowadays a major concern to many tropical wood researchers. The objective of this work was to assess the potentials of Jatropha curcas oil (JCO) alone and in combination with Cymbopogon citratus (JCO + CC), Ocimim basilicum (JCO + OB), and Eucalyptus globulus (JCO + EG) leaves extracts at different concentrations (1, 2, 4, 8, and 16 mg) as wood preservatives of Ayous (Triplochiton scleroxylon) against Macrotermes bellicosus termites. The extracts were tested for their toxicity and repellency on termites. Subsequently, treated and untreated wood was exposed to termites for four weeks. Termite response to treatùents varied with plant species, concentration, and exposure time. Ocimum basilicum extracts combined with J. curcas oil appeared to be the most effective against termites at 16 mg/l for in-vitro tests. It was also shown that the tested treatments were repellent to termites and therefore their mortality could not result from inhalation. J. curcas oil significantly improved the persistence of the treatments. Finally, a combined treatment of J. curcas oil and methanolic extracts of O. basilicum yielded the best protection of Ayous to termite attack, as shown by an average mass loss of 2%. Further research is needed to improve the longevity of the treatments on wood over time.

Inventory and Management of Fungi Associated with Banana Plant through the Use of &amp;lt;i&amp;gt;Allium ampeloprasum&amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;Cymbopogon citratus&amp;lt;/i&amp;gt; Extracts

Despite the nutritional, economic and medicinal values of banana plant, independent of the region and production system is confronted with some diseases such as the fungi disease. These fungal diseases are responsible for the low yields. The objective of this study was to improve the sanitary state of banana plant. To achieve this objective, fungi associated with banana leaves were isolated on Potato Dextrose Agar (PDA) culture medium and their identification was done on the basis of morphological and microscopic characteristics using reference documents. Antifungal activity of Allium ampeloprasum and Cymbopogon citratus extracts were evaluated in vitro on agar medium on the development of Pseudocercospora fijiensis, P. musicola and Pestalopsis sp. The results showed that banana plant harbours a diversity of fungal species, the most frequent being P. fijiensis (51.58%), Pestalopsis sp. (15.47%) and P. musicola (12.03%). Aqueous extracts of C. citratus at the concentration of 15 mg/ml, inhibited 100% of the radial growth of P. fijiensis and Pestalopsis sp with a fungitoxic activity. Similarly, ethanolic extract A. ampeloprasum inhibited at 100% the radial growth of Pestalopsis sp. This antifungal activity was fungistatic. These results suggest that the aqueous and ethanol extracts of the tested plants could be used as alternatives to chemical products in the fight against banana diseases especially Sigatoka. Hence further studies need to be undertaken to isolate the active compounds from these extracts with fungicidal potential.

Effect of Cymbopogon citratus on biofilm production by multidrug resistant Acinetobacter baumannii

Introduction and Aim: Acinetobacter baumannii, is the cause of many nosocomial infections which poses a serious threat to patients. Being a highly resistant organism, it has been placed in the critical priority list by WHO in 2017, thereby requiring newer effective drugs and alternative strategies. Increased antibiotic resistance has made it difficult to treat patients infected with this organism. The study aimed to find the biofilm forming ability of multidrug resistant A.baumannii causing respiratory tract infections, to find the genes responsible for biofilm production and to find the effect of Cymbopogon citratus (lemon grass) extract on biofilm. Materials and Methods: The antibiogram profile was studied for the Acinetobacter isolates obtained from endotracheal aspirates, endotracheal tubing tips by antibiotic susceptibility testing and Vitek-2. Genes known to be associated with biofilm production, (AbOmpA, Bap) in Acinetobacter were detected by Polymerase chain reaction (PCR). The Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), Minimum Biofilm Inhibitory Concentration (MBIC), Minimum Biofilm Eradication Concentration (MBEC) of Cymbopogon citratus essential oil was determined by microtiter plate method. Results: Isolates that were tested were found to be Multidrug resistant (MDR) and Extremely drug resistant (XDR) with highest resistance to Carbapenems (95.83%). Activity of Cymbopogon citratus EO was determined. The isolates predominantly showed MIC of 2% and MBC was 8%. MBIC was found to be 1%. However, Cymbopogon citratus EO did not eradicate the preformed biofilms. All 24 isolates showed AbOmpA gene and six showed presence of Bap gene. Conclusion: This study demonstrated that isolates that produced the Bap gene were Extremely drug resistant (XDR). Biofilm formation was inhibited by Lemongrass essential oil (EO) but it did not eradicate the preformed biofilms.