Cassava Extracts Research Articles

Isolation and Identification of Yeasts from Tomato (Solanum lycopersicum) Fruit and Cassava (Manihot esculenta) Tuber

Different species of yeast inhabit various plant types and parts owing to variations in their nutrient needs. Thus, an effort to identify ideal yeast species for a given industrial process would require a certain level of research input directed toward their habitats. Therefore, this study aimed to identify and isolate yeast cells from the fruit and tuber of tomato and cassava respectively. Yeasts were isolated from the extract of cassava and tomato tuber and fruit respectively. Morphological, biochemical and microscopic assessments were determined using standard procedures. Results obtained from this study showed Saccharomyces constituted (66.7%) of the microbial populations, while Candida constituted (33%) of the sample. In conclusion, it can be deduced from this study that tomatoes can be relied upon as a dependable source of Saccharomyces for certain industrial processes such as the production of brewery products.

Behavioural Activity and Renal Functions from Toxicity Level of Bitter Cassava Induced-Konzo and Linamarine

Background: Cassava (Manihot Esculenta) is the most widely consumed starch- based food in Africa, where it thrives in the continent's tropical and subtropical temperatures. Aim: The purpose of this research was to better understand the impact of linamarine using a rat model of konzo. Methods: Twenty-five (25) adults female Wistar rats were split into five (5) groups, with each group receiving the same amount of linamarin over the course of five weeks: group 1 received animal feed and water as a control, group 2 received bitter cassava and water, group 3 received 4.7 ml of linamarin, group 4 received 2.7 ml of linamarin, and group 5 received 0.7 ml of linamarin. The animals were killed so that samples could be tested in a lab. The time taken to go from the cage's entrance to each of its four corners was the metric tracked. Lack of movement, sluggishness, frequent urine and diarrhea, head sniffing and exploration, ears bent backward, puffiness, and teeth chattering were also seen. Results: The data revealed that the bitterness of the cassava was significantly reduced in the linamarin group compared to the control group, and that the participants in the linamarin group gained weight. The group fed linamarin had much fewer crossings, shorter rearing times, and lower motivation than the other groups, the group fed bitter cassava remained on the rod for longer at the end of the test. Conclusion: Research shows that consuming unprocessed bitter cassava results in renal impairment and that consuming pure linamarine, cassava extract induces hepatoxicity.

LIQUID SOAP FORMULATION FROM ETHANOLIC EXTRACT OF CASSAVA LEAVES (Manihot esculenta Crantz) WITH COCAMIDE DEA AS SURFACTANT

The ethanol extract of cassava leaves contains various secondary metabolites that have antibacterial activity against Escherichia coli, Staphylococcus aureus, and Shigella sp., and thus can be used as active ingredients in liquid soap preparation. Liquid soap formulations generally use sodium lauryl sulfate (SLS) as a surfactant, which can cause irritation and skin dryness. This study aimed to formulate an ethanol extract from cassava leaves into a liquid soap preparation using cocamide DEA as a surfactant to reduce skin irritation. The study was conducted in an experimental laboratory. Cassava leaves were extracted by maceration using ethanol. Three liquid soap formulas from cassava leaf extract were developed with different extract concentrations: F1 (1%), F2 (3%), and F3 (5%). The liquid soaps were evaluated in terms of organoleptic properties, pH, homogeneity, viscosity, foam height, and foam stability. The study showed that cassava leaves extract could be formulated into a homogeneous liquid soap with a pH range of 7.0–7.5, viscosity of 143.6–306.2 cps, foam height of 53.33–60.00 mm, and foam stability of 75.01–82.36%. F3 is the optimum formula with the best physical characteristics of the preparation in terms of organoleptic properties, foam height, and foam stability. Keywords: cocamide DEA, cassava leaves, liquid soap, surfactant

Harnessing the anti-cancer potential of linamarin: A computational study on design and hydrolysis mechanisms of its derivatives

Cassava extracts containing cyanogenic compounds demonstrate anticancer properties. The cyanogenic glucoside linamarin found abundantly in cassava can release hydrogen cyanide (HCN) upon hydrolysis, a potent cytotoxin. However, linamarin's hydrolysis mechanism by human enzymes is poorly delineated and constitutes a bottleneck for therapeutic development. This study aimed to investigate linamarin's hydrolysis mechanism by human β-glucosidase and identify structural derivatives with enhanced hydrolytic potential using density functional theory calculations. Results revealed α-anomeric derivatives as promising, with leaving group ability and steric bulk strongly governing hydrolysability. We identified several linamarin analogs with predicted rapid hydrolysis kinetics that may enable swift cytotoxic HCN release against cancer cells. This investigation enriches understanding of cyanogenic glycoside reactivity to facilitate their development as targeted antineoplastic agents. The identified derivatives set the groundwork for experimental evaluation of enhanced linamarin-inspired compounds as innovative cancer therapeutics.

Evaluation of physical and combustion characteristics of briquettes of palm fruit fibre and maize chaff using cassava extract as binder

The selection or choice of agro-waste briquettes for domestic and industrial cottage applications depends on the fuel properties. In this work, Palm fruit fibre (PFF) and maize chaff were separately sun dried for seven days to remove moisture. The dried materials were separately ground using electric milling machine. They were then reduced to mesh sizes of 1.18, 1.70, 2.36 and 3.35 mm respectively. Filtered liquid extract of cassava was collected and dried for five days to form the binder. Different concentration of biomass materials were prepared by blending palm fruit fibre at 20, 40, 60 and 80% concentrations with maize chaff. Shattering index, ignition time, water boiling time, burning rate, specific fuel consumption, percentage ash content and calorific values of PFF and maize chaff briquettes were investigated. Results showed that shattering index reduced with increase in particle size and increased with reduction in particle size. Highest and lowest durability were obtained at particle sizes of 1.18 and 3.35 mm respectively. The ignition time decreased with increase in particle size of briquettes. 1.18 mm particles give the highest ignition time (54.88 sec.) and 3.35 mm particles gave the lowest ignition time (24.24 sec.). Therefore, increasing the particle size increases the ignitibility of the briquettes. It was observed that the ignition time increases with increase in percentage composition of palm fruit fibre in the briquette samples with a maximum ignition time of 29.66 seconds at 80% PFF. The specific fuel consumption, shows that there was an initial increase from 50.0 g/ltr to 55.0 g/ltr, after which it decreased continuously with increase in particle size. Water boiling time, showed an inverse relationship with particle size. However, water boiling time between 2.36 and 3.35mm particle size has a more pronounced variation in comparison with preceding values. Higher calorific value 31,514.05 and 31,004.30 kJ/kg of PFF and maize chaff briquettes were recorded.

Uji Efikasi Berbagai Media Agens Antagonis Paenibacillus polymyxa Terhadap Penyakit Bacterial Leaf Blight Pada Tanaman Padi (Oryza sativa)

An experiment was carried out in Margasari Village, Dawuan District, Subang Regency, from July 2020 to November 2020. The purpose of this study was to study the efficacy of various media of the antagonist agent Paenibacillus polymyxa in controlling Bacterial Leaf Blight in rice plants and to find the most effective and efficient media. to control Bacterial Leaf Blight in rice plants. The method used in this study was an experiment through field experiments, with a simple randomized block design consisting of six treatments and four replications. The experimental treatments consisted of: A (Sugar Potato Extract), B (Sweet Potato Extract), C (Sugar Cassava Extract), D (Soybean Extract), E (Sugar Rice Wash Water) and F (Control). The experimental results show that: (1) All the Paenibacillus polymyxa antagonist agent media in this experiment were effective in suppressing the intensity of the attack of Bacterial Leaf Blight (2) The more effective Paenibacillus polymyxa antagonist agent medium was treatment A (Sugar Potato Extract) which was able to suppress the intensity. attack was lower than the other treatments, so it had the most number of productive tillers and produced the highest average productivity of 8.08 tons ha-1.

Consumption of Cassava Extract (Manihot Esculenta) Improves Pancreatic Histology and Seminiferous Tubules in Wistar Rats Induced Diabetes Mellitus with Streptozotocin

Consumption of Cassava Extract (Manihot Esculenta) Improves Pancreatic Histology and Seminiferous Tubules in Wistar Rats Induced Diabetes Mellitus with Streptozotocin

Antioxidant Activity of Flavonoids from Cassava Leaves (Manihot esculenta Crantz)

The community uses cassava leaves for cooking because of their high protein content, β-carotene, Fe, Mg, Zn, S, Ca, Zn, Ni, and K. Cassava leaves contain various secondary metabolites, including flavonoids, saponins, tannins, phenolics, steroids, anthocyanins, and anthraquinones. The antioxidant activity of flavonoid compounds in cassava leaves (Manihot esculenta Crantz) has been successfully investigated. This study consisted of six stages: the preparation of ethanol extract of cassava leaves, phytochemical screening, determination of total flavonoid content in the extract, isolation of flavonoids, and purity testing of flavonoid isolates using the chromatographic method, identification of the structure of pure flavonoid isolates using a UV-Vis spectrophotometer with the addition of a shear reagent, FTIR, and LC-MS/MS. As well as test the antioxidant activity using the DPPH method. The yield of ethanol extract from cassava leaf in this study was 14.67%. The results of the phytochemical screening showed that the cassava leaves and the ethanol extract of cassava leaves contained alkaloids, flavonoids, saponins, tannins, quinones, steroids, phenolics, and triterpenoids. The total content of flavonoids in the ethanol extract was 35.71 mg EQ/g extract. UV-Vis, FTIR, and LC-MS/MS analysis revealed that the flavonoid isolate was quercetin. The results of the antioxidant activity of the ethanol extract and BC isolate showed IC50 of 81.76 ± 0.505 mg/L and 77.85 ± 4.708 mg/L.

Antibacterial activity of ethanol extract of banana, cassava, and pineapple peels against a fish pathogen Aeromonas hydrophila

Abstract. Mulia DS, Raicha R, Lunggani CP, Erina SN, Wuliandari JR, Purbomartono C, Isnansetyo A. 2023. Antibacterial activity of ethanol extract of banana, cassava, and pineapple peels against a fish pathogen Aeromonas hydrophila. Biodiversitas 24: 481-485. Aeromonas hydrophila is the most virulent bacteria for almost all freshwater fish species. One method to control pathogenic bacteria in fish should be safe and environmentally friendly, including utilizing natural wastes such as banana peels (Musa paradisiaca), cassava peels (Manihot esculenta), and pineapple peels (Ananas comosus). These natural wastes contain bioactive compounds. Their utilization increases the waste value. This study aims to determine the potential of banana peel, cassava peel, and pineapple peel waste as an antibacterial against A. hydrophila, a causing agent of Motile Aeromonas Septicemia (MAS) in freshwater fish. Parameters observed included phytochemical compounds, MIC, and MBC values of banana, cassava, and pineapple peels. Phytochemical screening was performed using thin-layer chromatography (TLC) and foam test. The microdilution method carried out the antibacterial activity of banana peel, cassava peel, and pineapple peel against A. hydrophila. It determines the minimum inhibitory concentration (MIC); and the minimum bactericidal concentration (MBC) values. Phytochemical screening results showed that banana, cassava, and pineapple peel extracts contained flavonoids, alkaloids, terpenoids, tannins, and saponins. The MIC values of banana peel, cassava peel, and pineapple peel extract were 600, 400, and 200 µg/mL, respectively. The MBC values of banana peel, cassava peel, and pineapple peels were 4 × MIC, 2 × MIC, and 1 × MIC, respectively. Extract of pineapple peel exhibited better antibacterial activity than banana and cassava peels. However, the three waste extracts indicated the antibacterial potential to be developed as natural bactericides to control A. hydrophila bacterial disease.

Cassava (Manihot esculenta Crantz): A Systematic Review for the Pharmacological Activities, Traditional Uses, Nutritional Values, and Phytochemistry.

Cassava (Manihot esculenta Crantz) is considered one of the essential tuber crops, serving as a dietary staple food for various populations. This systematic review provides a comprehensive summary of the nutritional and therapeutic properties of cassava, which is an important dietary staple and traditional medicine. The review aims to evaluate and summarize the phytochemical components of cassava and their association with pharmacological activities, traditional uses, and nutritional importance in global food crises. To collect all relevant information, electronic databases; Cochrane Library, PubMed, Scopus, Web of Science, Google Scholar, and Preprint Platforms were searched for studies on cassava from inception until October 2022. A total of 1582 studies were screened, while only 34 were included in this review. The results of the review indicate that cassava has diverse pharmacological activities, including anti-bacterial, anti-cancer, anti-diabetic, anti-diarrheal, anti-inflammatory, hypocholesterolemic effects, and wound healing properties. However, more studies that aim to isolate the phytochemicals in cassava extracts and evaluate their pharmacological property are necessary to further validate their medical and nutritional values.

UJI DAYA ANALGETIK DAN ANTIINFLAMASI EKSTRAK ETANOLIK DAUN SINGKONG (Manihot utilissima Pohl.) PADA MENCIT GALUR SWISS

Background: Flavonoids are compounds that can function as analgesics and anti-inflammatory. Cassava leaves are known to contain flavonoids with the main content of rutin. The routine flavonoid content in the ethanolic extract of cassava leaves is 4.987% so that it has the potential to be developed as traditional medicine. Objective: This study aimed to determine the analgesic and anti-inflammatory properties of cassava leaf ethanolic extract (EEDS) and the effect of varying doses of EEDS on analgesic and anti-inflammatory properties. Methods: This study was a completely randomized experimental study with a unidirectional pattern in mice aged 2-3 months and weighing 20-30 grams. Analgesic testing method used the stretching method with 1% acetic acid as the induction, while the anti-inflammatory test used the edema thickness measurement method with 1% carrageenin induction. The results of the percentage of protection, the change in the percentage of protection, and the percentage of anti-inflammatory power were statistically analyzed. Results: The results showed that EEDS was green-black in color, bitter in taste, 7% drying loss, 12.31% w/w yield and positive for flavonoids. EEDS has analgesic power at a dose of 70; 140; and 280 (mg/kgBB) with a percentage of protection of 26.59%; 24.45%; and 21.96%; change in percentage of protection by 65.75%; 68.51%; and 71.72%. EEDS at a dose of 35; 70; and 140 (mg/kgBW) has a percentage of anti-inflammatory power of 44.93%; 37.34%; and 30.75%. Conclusion: The higher the dose of EEDS, the higher the analgesic power but the lower the anti-inflammatory power

<i>Leucoagaricus gongylophorus</i> provides protection for </i>Atta sexdens</i> against plant extracts

Plant extracts have been considered for the control the leaf-cutting ants. The objectives of this study were to evaluate the survival leaf-cutting worker ants after topical application of Ageratum conyzoides (mentrasto) and Manihot esculenta (cassava) extracts; and to evaluate the effect of Manihot esculenta hexane extract via ingestion on the survival of the workers of Atta sexdens Forel 1908 isolated or not from their colonies, in order to verify whether the symbiotic fungus Leucoagaricus gongylophorus confers protection to the workers against the extract. First, ten medium workers were removed from their colonies and received the application of 1 μL of extract on the pronotum. The cassava extract (125 µg.mL-1) was diluted in a solution of honey with water for the ingestion bioassay. Five repetitions (ten ants inside a transparent plastic container) were performed in each of the four colonies used in the experiment. The numbers of dead ants were recorded daily until the control also died. The solution of the hexane Manihot esculenta extract gave a significant result at the concentration of 100 µg.mL-1, with 100% of mortatily after 4 days. The ants maintained with the symbiont fungus that ingested the cassava hexanic extract showed a lower mortality rate (40% at the end of 25 days) than the workers who were kept isolated (90%). The symbiosis between the ants and the fungus means more than a food source for leaf-cutting ants because it reduces the toxic effect on the ants when the ants remain in contact with the fungus and is offered an unsafe vegetable.

Nutrition, Healthcare Benefits and Phytochemical Properties of Cassava (Manihot esculenta) Leaves Sourced from Three Countries (Reunion, Guinea, and Costa Rica)

(1) Background: Manihot esculenta, cassava, is an essential food crop for human consumption in many parts of the world. Besides the wide use of its roots, cassava leaves have been used locally as green vegetables and for medicinal purposes. However, nutritional health data regarding cassava leaves is limited, therefore we investigated its composition and associated potential bioactivity interest for human health. (2) Methods: Cassava leaf bioactivity investigations focused on antioxidant properties (free radical scavenging) in association with immunomodulatory activities on inflammatory murine macrophages to measure the impact of cassava extract on the production of pro-inflammatory cytokines such as Interleukin-6, Tumor Necrosis Factor alpha, Monocyte Chemoattractant Protein-1, Prostaglandin-E2 and mediators such as nitric oxide. (3) Results: Antioxidant and immunomodulatory bioactivities were significant, with a concentration-dependent inhibition of cytokines production by inflammatory macrophages; (4) Conclusions: Taken together, our results tend to suggest that Manihot esculenta leaves might be underrated regarding the potential nutrition-health interest of this vegetal matrix for both human nutrition and prophylaxis of metabolic disease with underlying low grade inflammation status.

Allelopathic Potential of Cassava (Manihot esculenta L.) Extracts on Germination and Seedling Growth of Selected Weeds and Aerobic Rice

Weed infestation is a major problem in the aerobic rice system due to the lack of standing water that could prevent the growth of weeds. To reduce heavy reliance on herbicide, this research aims to determine the potential of cassava allelopathy for inhibition of weeds in aerobic rice. The allelopathic potential of cassava extracts on the germination and growth of tested weed species (Eleusine indica, Ageratum conyzoides, and Cyperus distans) and aerobic rice (Oryza sativa) was conducted in the laboratory. The results showed that increasing the aqueous extract concentrations of cassava extracts inhibited the germination and seedling growth of tested weeds, suggesting the allelopathic effects of cassava extracts are concentration dependent. The degree of phytotoxicity of different vegetative parts of cassava can be classified in order of decreasing inhibition as follows: leaf, stem, tuber, and tuber peel. Aqueous leaf extract of cassava at a concentration of 0.5% (w/v) provided complete inhibition on A. conyzoides, E. indica, and C. distansgermination whereas 25% to 100% inhibition on the shoot growth was recorded. By contrast, the shoot growth and germination of aerobic rice were not affected. These results suggest that the cassava leaf extracts contain water-soluble allelochemicals for inhibition on A. conyzoides, E. indica, and C. distans in aerobic rice.

Antioxidant activity of methanol extract of cassava leaves (Manihot esculenta Crantz) using CUPRAC method

Background: Antioxidants are a group of organic molecules and enzymes that work synergistically to enhance cellular defenses and combat oxidative stress. Antioxidants can come from plants because they contain many groups of phytochemical compounds and vitamins. One of the plants that has compounds with antioxidant activity is cassava. Objective: To measure the antioxidant activity of methanol extract of cassava leaves using the CUPRAC method. Methods: Cassava leaves were extracted by maceration using methanol as a solvent with the addition of 5% H2SO4. Phytochemical screening of flavonoids with Mg and HCl, while phenol with the addition of FeCl3. The antioxidant activity based on the reaction of the test solution with CUPRAC reagent. Antioxidant activity can be determined by IC50 value by using x value as concentration and y value as absorbance. Results: The results of phytochemical screening showed that the methanol extract of cassava leaves contained flavonoids and phenolic groups. The antioxidant capacity of the methanol extract of cassava leaves obtained an IC50 value of 156.03 ppm, while quercetin has a very strong capacity as an antioxidant with an IC50 of 9.83 ppm. Conclusion: The methanol extract of cassava leaves has a weak antioxidant capacity in reducing metal ions. Keywords: Cassava leaves, Manihot esculenta Crantz, methanol extract, antioxidants, CUPRAC.

In Vitro Effect of Aqueous Extract of Fresh Cassava Leaves (Manihot Esculenta, Crantz) on The Larvae of Haemonchus Contortus as an Intestinal Parasite of Sheep

Cassava is frequently fed to animals. In the case of sheep, the producer relates consumption to a reduction in the parasite load. The literature has proven the effect of phenolic compounds as an anthelmintic in vivo, but no evidence for cyanogenic compounds, also present in all parts of the cassava plant, was found. A controlled in vitro bioassay was used to evaluate the aqueous extract of fresh cassava leaves. The efficiency parameter was the immobility of Haemonchus contortus larvae at the L3 stage, also used to evaluate commercial anthelmintics. Cell culture plates with 100 active L3-stage larvae per well were used, being each replicate constituted by three wells. Aqueous extract of fresh cassava leaves (FCL), Ivomec® 0.01% (PCI) as the positive control, and distilled water as negative control (NCW), were placed in the culture plate wells. Considering the immobility of the larvae as a positive anthelmintic effect, the results showed that in NCW treatments all larvae were mobile, while in PCI all 300 larvae were immobile. FCL produced a gradient of larval inactivation correlation (R2 0.996). The best-fit equation was y = -33.39ln(x) + 40.517, a logarithmic equation, which allowed the calculation of the Lethal Concentration (CL) of 3.44 µg CN- ml, or 80.0 mg of fresh cassava leaves per milliliter of water, with a performance equivalent to ivermectin. This concentration of free cyanide is compatible with the safe consumption of fresh leaves by live weight of sheep. The exact amount of cassava roots, leaves, or shoots, consumed to provide an effective dose for controlling H. contort should be established in vivo. Although phenolic compounds must also be present in the extract, the immobility was attributed to cyanogenic compounds since the correlation was proportional to the increase in the cyanide concentration. It can be concluded that the consumption of fresh cassava leaves has the potential as an anthelmintic agent to be evaluated in vivo by feeding sheep and goats. Local use could also add value to the production of fresh cassava leaves, with an average potential production of 2.5 tonnes ha-1, available throughout the year, with greater production at the beginning of cultivation and in the summer months. These leaves could be used after the roots harvesting or even after pruning for this purpose. Currently, this amount of good quality protein material remains without use in the field.

By-product Utilization of Eri Silkworm Host Plant Tapioca (Manihot esculenta Crantz.): A Review

Manihot esculenta Crantz., cassava also called tapioca, is a woody tuberous shrub, native to South America. It belongs to the family Euphorbiaceae. The leaves of tapioca plant serve as a secondary host plant in eri culture, i.e. rearing of eri silkworm, Samia ricini Donovan, particularly practiced in the north-eastern region of India. Besides the utilization of tapioca tubers for human consumption and as feed for animals, the leaves and tubers of this wonder plant can be used for diversified purposes as in food industry, pharmaceuticals, textile industry, cosmetic industry, explosive industry, paper industry, etc. The ethyl acetate extract of cassava can be used as a potential grain protectant against red flour beetle (Tribolium castaneum) and rice weevil (Sitophilus oryzae). Tapioca is not only of low cost but also serves as a wonder plant for sustainable future. The use and exploration of tapioca has much more vivid scope besides its utilization in eri silkworm rearing. In tapioca producing regions of India, tapioca can be utilized for dual purpose of eri silkworm rearing and for other industrial products which will help to grow tapioca based industry. It will also help for the sustainable development of eri silkworm rearers. Indigenous traditional knowledge (ITK) strongly proves that tapioca plants are endowed with pesticidal properties that can be widely explored in agriculture and related fields. Therefore, this article strongly supports and highlights the use and exploration of tapioca in various agricultural and industrial practices.

Formulation of Cassava (Manihot esculenta Crantz) Tuber Extract Gel and Effectiveness Test on Burns in Rabbit (Oryctolagus cuniculus)

Burn is heat transfer in which the source can be direct or indirect contact with fire, electricity, friction chemicals or radiation. It can cause damage of skin tissue even in a state of multisystem injury leading to serious disorders. Systemic effects and mortality caused by burn is generally determined by the extent and depth of affected skin. Cassava (Manihot esculenta Crantz) contains carbohydrates, phosphorus, calcium, vitamin C, vitamin B1, protein and iron. Furthermore, substances contained in cassava are able to give effect to the re-epithelialization of skin damaged cell tissue due to burns. The aim of this study was to find out the cassava tuber extract in gel preparation formulation and the effectiveness of burn healing in rabbits. This study was an experimental study to examine the concentration of cassava tuber extract gel formula which is the most effective in healing superficial burns of degree 4 in male rabbits. From the observations of giving gel preparations once a day by using positive control comparison (Bioplaceton) could be concluded that the extract of cassava provided healing which was not significantly different from the positive control.

Naturally Acquired Lactic Acid Bacteria from Fermented Cassava Improves Nutrient and Anti-dysbiosis Activity of Soy Tempeh

Introduction: Gut microbiota dysbiosis indicated by increased gram-negative bacteria and reduced Firmicutes-producing short chain fatty acids bacteria has been linked with impairment in glucose metabolism. Tempeh is traditional fermented soy food that can stimulate the growth of beneficial bacteria. In Indonesia, some tempeh was produced by adding acidifier that contains lactic acid bacteria. This process may impact the nutrient and anti-dysbiosis activity of tempeh. 
 Objectives: To evaluate the impact of acidifier on nutrient and gut microbiota profile of diabetic animal model. 
 Method: Modified tempeh was made by addition of water extract of fermented cassava. Standard and modified tempeh were subjected to proximate analysis and dietary fibre. Diabetic animals were received standard tempeh or modified tempeh diet replacing 15% and 30% of protein in the diet for 4 weeks of intervention. At the end of experiment, caecal content was collected. Short chain fatty acids and microbiota composition were analysed using 16s rDNA next generation sequencing (NGS).
 Result: There is significant different (p<0.05) on fat, protein, water and dietary fibre content between regular soy tempeh and modified tempeh. There is significant different (p<0.05) on serum glucose and short chain fatty acid composition among group. Diabetic animal has low ratio of Firmicutes/Bacteroidetes. Supplementation of both tempeh increased bacterial diversity, Firmicutes /Bacteroidetes ratio and short chain fatty acids producing bacteria. 
 Conclusion: Addition of naturally occurred lactic acid bacteria from fermented cassava during tempeh processing improved both nutrient and microbiota composition in the gut of diabetes mellitus.

The potential of cassava leaves extract in gingival fibroblasts of periodontitis of rats with ovarian dysfunction

Introduction: The prevalence of periodontitis has reached 42.8% in Indonesia. One contributing factor is Porphyromonas gingivalis. The ovarian dysfunction caused by ovariectomy procedures may also cause periodontitis. Post ovariectomy conditions resemble menopause, lowering the production of estrogen and progesterone hormones, leaving patients vulnerable to periodontitis. To prevent the side effects of the treatment, herbal ingredients are commonly used. The extract of cassava leaves is known to have pharmacological effects as an antioxidant and, thus, can be applied as a medicine for periodontitis. Methods: A total of 21 rats were divided into three groups, (1) control: healthy rats; (2) Group P.1: P. gingivalis-induced rats; (3) Group P.2: ovariectomized rats. Groups P.1 and P.2 were further divided into three subgroups that were administered cassava leaves extract at a dosage of 179.2 mg/ kg BW, aquades and vitamin C. Rats were euthanized at day eight after the initial treatment to collect left mandibular first molar. The mandibular sections were immunohistochemically stained to quantify SOD expression using light microscope while the Image J software was used. Fibroblast cells expressing SOD are characterized by brown coloration in the cytoplasm and darker nucleus. Results: In periodontitis conditions, either due to P.gingivalis induction or ovarian dysfunction, fibroblast cells in rats given cassava leaves extract expressed higher SOD than rats given aquades, but still lower than rats given vitamin C. The result of the one way ANOVA test showed p value of 0,00, which means that there is a significant difference in all groups. Conclusion: Cassava leaves extract demonstrated the potential to increase the antioxidant expression in P. gingivalis-induced and ovarian dysfunction rats.