Aloe Barbadensis Miller Extract Research Articles

Bio-ultrasonic synthesis of tin oxide quantum dots: effect of bio-carbon and their UV photocatalytic activity

An ecofriendly synthesis is realized to elaborate tin oxide quantum dots (SnO2 -QDs) using the plant aqueous extract of Aloe Barbadensis Miller (Aloe Vera) and SnCl4.5H2O at room temperature as a biological solvent and a precursor respectively. The effect of Aloe Vera extract concentration on the properties of SnO2-QDs has been studied. Morphological and structural properties of the as synthesized nanoparticles have been characterized using field effect-scanning electron microscopy (FE-SEM) and x-ray diffraction (XRD). The chemical composition of the nanoparticles was studied by Raman, energy dispersive x-ray (EDX) and Fourier transformation infra-red (FTIR) spectroscopy. The optical properties were investigated by UV–Vis spectrophotometer. The x-ray diffraction analysis showed that all samples have a tetragonal rutile structure, with an estimated crystal size closed to the exciton Bohr radius, indicating a strong confinement of the carriers in the material. The crystallite size of SnO2-QDs nanoparticles decreases as Aloe Vera plant extract concentration increases. The formation of SnO2-QDs and the presence of graphitic carbon in samples were confirmed by Raman spectroscopy, EDX analysis and Fourier transformation infra-red (FTIR) spectroscopy. The blue shift in absorption is the most likely due to the quantum confinement effect. An Ostwald-repining growth model based on the concept of surface energy has been proposed to explain the kinetic growth of SnO2 QDs. The photocatalytic activities of the as-prepared powders were confirmed by the fast and efficient degradation of methylene blue (MB).

In silico docking studies of alpha-amylase inhibitors from the methanol extract of Aloe vera

In the current study, potent inhibitors of a-amylase, one of the significant regulating enzymes in diabetes, were identified from the methanolic extract of Aloe barbadensis miller (Aloe vera), an indigenous herbal plant used to treat carbohydrate absorption problems such as diabetes and obesity. The Aloe vera gel extract was dried, ground, macerated with methanol and subjected to phytochemical screening, which was characterized by gas chromatography. Since there is a rapid increase in computational methods to predict the association between two molecules, molecular docking studies assessed the effect of AVM (Aloe vera methanol) extract in inhibiting the enzyme alpha-amylase. The molecules derived from GC-MS analysis were employed as ligands for the receptor α-amylase in molecular docking experiments. The protein target used in this study was the human pancreatic α-amylase with PDB-ID -2QV4. The protein target and ligand structures were obtained from PDB and Pubchem databases respectively and docking studies were performed using PyRx. In this study, we intended to find distinct phytochemical substances from the Aloe vera plant as α-amylase inhibitors with an objective that the molecular docking studies provided in this study might result in the discovery of effective α-amylase inhibitors for diabetic management.

ANTI-INFLAMMATORY, ANTIPYRETIC AND ANALGESIC ACTIVITIES OF CHLOROFORM EXTRACT OF ALOE BARBADENSIS MILLER IN ALBINO RATS

The pharmacological activities of the leaf gel of Aloe vera have been extensively evaluated. Gel and latex are two basic products of aloe leaves. Latex and gel contain biologically active components. Polysaccharides contained in the leaf gel attribute most of the health benefits like anti-inflammatory, pain and fever, associated with aloe vera. In the present study, chloroform extract of A. barbadensis at various concentrations was investigated for its anti-inflammatory, antipyretic and analgesic activities in albino rats. Twenty-four albino rats were randomly divided into three groups (control, standard and experimental group). Division of groups was the same for all activities. Control and standard groups contain 4 rats in each group whereas experimental group contains 16. All the rats in three groups were treated with carrageenan to induce oedema, Brewer’s yeast to induce pyrexia and acetic acid to induce pain. The control group was treated with normal saline for all the activities. Standard group rats were treated with the reference drug diclofenac for anti-inflammatory and analgesic activities and paracetamol for antipyretic activity. Experimental group rats were given chloroform extract of A. barbadensis with 50, 100, 200 and 400mg/kg concentration. The result showed a significant inhibition (98%) in oedema at 3rd hour at the dose of 400mg/kg as compared to control group. For antipyretic activity, there was a significant reduction (66%) in pyrexia at 4th hour at the dose of 50mg/kg as compared to control group. And in analgesic model a significant reduction (64%) in the writhing at the dose of 400mg/kg as compared to control group. These results demonstrated that the chloroform extract of Aloe barbadensis miller have anti-inflammatory, antipyretic and analgesic activity and suggested its inhibitory actions on inflammation, fever and pain.

Study of Structural, Morphological and Optical Properties of In 2 O 3 Nano Powder Synthesised By Aloe Barbadensis Miller Extract

Study of Structural, Morphological and Optical Properties of In 2 O 3 Nano Powder Synthesised By Aloe Barbadensis Miller Extract

Geeta Bhandari and Shruti Baurai

Abstract-Exposure to sunlight can trigger various biological responses ranging from sun-burn, erythema to skin cancer. Synthetic sunscreen formulations available in market pose variety of adverse effects. Therefore,formulationofthe herbalsunscreenformulation and evaluation of its sun protection activity is an important aspect in the cosmetic industry. The purpose of present study was to evaluate the sun protection factor (SPF) of aqueous and methanolic extract ofAloe barbadensis miller andCocos nuciferaby ultraviolet (UV) spectroscopy method.Methanolic extract of Cocos nucifera showed the highest SPF value (3.2)amongst all extracts.The results indicated presence of active components responsible for ultraviolet absorption which may be extracted from these plant extractsand maybe used in sunscreens preparations for better protection against sun rays.Key Words: UVradiations, SPF, Aloe barbadensis miller andCocos nucifera

Assessment of In Vitro Sun Protection Factor of Plant Extracts by Ultraviolet Spectroscopy Method

Exposure to sunlight can trigger various biological responses ranging from sun-burn, erythema to skin cancer. Synthetic sunscreen formulations available in market pose variety of adverse effects. Therefore, formulation of the herbal sunscreen formulation and evaluation of its sun protection activity is an important aspect in the cosmetic industry. The purpose of present study was to evaluate the sun protection factor (SPF) of aqueous and methanolic extract of Aloe barbadensis miller and Cocos nucifera by ultraviolet (UV) spectroscopy method. Methanolic extract of Cocos nucifera showed the highest SPF value (3.2) amongst all extracts. The results indicated presence of active components responsible for ultraviolet absorption which may be extracted from these plant extracts and maybe used in sunscreens preparations for better protection against sun rays.

Synthesis of CuO samples by co-precipitation and green mediated combustion routes: Comparison of their structural, optical properties, photocatalytic, antibacterial, haemolytic and cytotoxic activities

The goal of this research is to prepare novel, inexpensive, environment friendly and efficient crystalline CuO samples by green mediated combustion and co-precipitation routes towards environmental remediation and biomedical applications. The influence of preparation routes and Aloe barbadensis miller (Aloe vera) gel on the morphology, size, crystallinity, band gap, defects, photocatalytic, antibacterial, haemolytic and anticancer behaviour of CuO samples were explored. The PXRD, XPS, SEM, TEM, HRTEM and FT-IR were performed to confirm the formation of CuO samples. FTIR studies showed all possible bands of Aloe barbadensis miller gel. FTIR displaying the occurrence of new peaks and peak shifts in the CuO synthesized by green mediated combustion route compared to co-precipitation route is an evidence of the effective interactions between CuO and Aloe vera gel. The PL and UV–Vis-DRS techniques measured the optical sensitivity and tuning of band gap of the CuO samples. The crystallinity and surface properties dependent photocatalytic activities for decolourization of MB and RhB under both UV and Sunlight irradiation were investigated. The results indicated that green mediated synthesized CuO sample displayed high photocatalytic activity compared with co-precipitation route synthesized CuO sample, which mainly resulted from the low crystallinity and crystallite size (6 nm), narrow band gap (1.73 eV) and lower recombination of charge carriers. These synthesized CuO samples also demonstrate excellent antibacterial activity against the bacteria Gram-positive (S. aureus) and Gram-negative (E. coli). The present study probes into the cytotoxicity of CuO samples employing root cells of Allium cepa. In addition, we report the haemolytic activity on goat and human blood along with the implementation of green synthesized CuO sample using an Aloe barbadensis miller extract for the evaluation of anticancer activity in human cervical cancer (HeLa) cells with IC50 value of 310.1 μg/ml. Our studies focus on developing biosynthesized nanomaterials for environmental remediation and biomedical applications.

Phytochemicals and Morphological Influence of Aloe Barbadensis Miller Extract Capped Biosynthesis of CdO Nanosticks

The present work reports the biosynthesis of CdO nanosticks by the green method using Aloe Barbadensis Miller (ABM) extract and it acted as a capping and stabilizing agent. This type of prepared sample was investigated by some standard studies. Crystallite size (28 nm) and structure (SC) were analyzed from XRD pattern. The optical properties of CdO nanoparticles are analyzed by UV–Vis–DRS and PL spectra. The FT-IR spectra exhibit the functional group present in the CdO nanosticks prepared from ABM extract due to its vibrational property. GC–MS analysis predicts the 35 phytochemicals present in the ABM extract. The surface morphology and elemental were investigated by HRTEM, FESEM and EDAX analysis and it displays a nanosticks structure. The nanosticks formation mechanism was also described. The antibacterial and fungal activities of ABM extract CdO nanoparticles are tested for different bacterial and fungal organism under the zone inhabitation method.

Kinetics and thermodynamic studies for removal of methylene blue dye by biosynthesize copper oxide nanoparticles and its antibacterial activity.

The present study deals with the green approach for the biosynthesis of copper oxide-Aloe vera (CuO-A) based nanoparticles using leaf extract of Aloe barbadensis miller. Synthesized nanoparticles were characterized through different techniques like TEM and FTIR. As the size decreases and surface area increases, these are prominently used as a very good adsorbent. The effects of different parameters like adsorbent dosage, pH, contact time, initial dye concentration and temperature are optimised to get the maximum removal of methylene blue dye from the solution. The maximum dye removal was found to be 98.89% with initial concentration of 100mg/L at alkaline pH in 210min., with shaking speed of 150rpm. The Langmuir result reveals a better consistency than the Freundlich model with 95.5mg/g. Lagergren's model was used to study the kinetics of the system. Mechanistic behaviour was study through intra-particle diffusion study and Boyd plot. Thermodynamic study showed spontaneous and endothermic nature of the adsorption. Furthermore, synthesized CuO-A nanoparticles showed good antibacterial activity against different strains of bacteria. The zone of inhibition was found to be 11mm, 12mm, 8mm and 9mm in Pseudomonas, Klebsiella, Staphylococcus and E.coli, respectively.

Calcium extraction and synthesis of precipitated calcium carbonate from Mg-rich dolomite

Abstract A green-reaction assisted synthesis using a natural occurring dolomite is reported for precipitated calcium carbonate production over aloe barbadensis miller extract template under ambient condition. Uniform, hierarchical aragonite precipitated calcium carbonate stacked from nanoparticles was synthesized. The tainted Mg influence on resultant PCC purity was averted via D-glucose extraction of dolomite constituent calcium and subsequent precipitation using CO2. The as-synthesized PCC was characterized using SEM, XRD, PSD, and FTIR spectroscopy. Under ambient conditions using carbonation route and 1 L/min CO2 flowrate, aragonite polymorph with hierarchical flower-like structure was produced. Energy is conserved as heat is not required for phase transformation. Minerals & Environment (RAMM) 2018.

Treatment of rats anal fistula with glycerin Aloe barbadensis Miller extract

RationaleThere is a need for a low cost treatment for complex anal fistulas that can heal without causing fecal incontinence, with room for the study of herbal medicines. ObjectiveTo evaluate the effect of the glycerin Aloe barbadensis Miller extract in the treatment of anal fistula in rats. MethodThirty Wistar rats underwent peritoneal anesthesia with ketamine and Xylasine, followed by transfixation of the anal sphincter with a steel wire, which remained for 30 days to develop the anal fistula. After this period, the steel wire was removed and three groups with 10 animals were formed: A (control), without treatment; B (seton), in which a cotton seton was introduced; C (therapeutic seton), in which a cotton thread was introduced as a seton, and Aloe barbadensis Miller extract was daily dripped in the seton; after 30 days, the setons of groups B and C were removed, and after two weeks without a seton euthanasia and removal of specimens was conducted, and the fistula closure and the inflammatory process were analyzed. ResultsFistula persistence was observed in all animals in Group A, six in Group B and three in Group C (p = 0.015). The mean local inflammation levels were 0.9 in Group A; 0.8 in Group B, and 0 in Group C (p = 0.015). ConclusionThe use of a seton soaked in a glycerin Aloe barbadensis Miller extract was effective in the healing of anal fistulas in rats, in addition to causing less inflammatory process than the non-phytotherapeutic seton.

DESENVOLVIMENTO DE SABONETE LÍQUIDO DE ALOE VERA: EXTRAÇÃO, PURIFICAÇÃO DO EXTRATO ATIVO E MANIPULAÇÃO DA FORMA FARMACÊUTICA

The use of herbs in alternative medicine has been a common practice. Aloe barbadensis miller , popularly known as aloe vera is a plant used since ancient times, dating back to its first use in Egypt in the year 400 D.C. This plant has in its composition chemical entities that act as agents for the adjuvant treatment of many pathologies, in addition to having antibacterial, antifungal and anti-inflammatory. The central proposal of this study was to develop a hypoallergenic soap for dermatological use, upon validation of a new extraction method using the active extract of Aloe barbadensis Miller in propylene glycol and glycerin. To do this, macerations were performed using reagents such as propylene glycol and glycerin extractors. The extract obtained from glycerine had high viscosity compared to the extract using propylene glycol. Macerations conducted in both results were favorable to the following pharmaceutical. However, it was observed that the final product from the reactant propylene glycol, was easier to homogenization, and therefore more economically viable to the market. It is suggested to carry out specific biological tests that effectively prove the efficiency of the antimicrobial soap developed.

Mushroom tyrosinase inhibitors from Aloe barbadensis Miller

Two new chromones, 5-((S)-2′-oxo-4′-hydroxypentyl)-2-(β-glucopyranosyl-oxy-methyl)chromone (1) and 5-((S)-2′-oxo-4′-hydroxypentyl)-2-methoxychromone (2), together with four known analogues, 8-C-glucosyl-7-O-methyl-(S)-aloesol (3), isoaloeresin D (4), 8-C-glucosyl-(R)-aloesol (5), and aloesin (6) were isolated from the aqueous extract of Aloe barbadensis Miller. Their structures were determined on the basis of spectroscopic evidences (1-D and 2-D NMR, HRMS, UV, and IR data), chemical methods and the literature data. The Mosher's method was applied to establish the absolute configuration of compounds 1 and 2. The inhibitory effects of these chromones on the activity of mushroom tyrosinase were examined, and compound 6 was identified as a noncompetitive tyrosinase inhibitor with an IC50 value of 108.62μg·mL−1.

Clear evidence of carcinogenic activity by a whole-leaf extract of Aloe barbadensis miller (aloe vera) in F344/N rats.

Aloe barbadensis Miller (Aloe vera) is an herbal remedy promoted to treat a variety of illnesses; however, only limited data are available on the safety of this dietary supplement. Drinking water exposure of F344/N rats and B6C3F1 mice to an Aloe vera whole-leaf extract (1, 2, and 3%) for 13 weeks resulted in goblet cell hyperplasia of the large intestine in both species. Based upon this observation, 2-year drinking water studies were conducted to assess the carcinogenic potential of an Aloe vera whole-leaf extract when administered to F344/N rats (48 per sex per group) at 0.5, 1, and 1.5%, and B6C3F1 mice (48 per sex per group) at 1, 2, and 3%. Compared with controls, survival was decreased in the 1.5% dose group of female rats. Treatment-related neoplasms and nonneoplastic lesions in both species were confined primarily to the large intestine. Incidences of adenomas and/or carcinomas of the ileo-cecal and cecal-colic junction, cecum, and ascending and transverse colon were significantly higher than controls in male and female rats in the 1 and 1.5% dose groups. There were no neoplasms of the large intestine in mice or in the 0 or 0.5% dose groups of rats. Increased incidences of mucosa hyperplasia of the large intestine were observed in F344/N rats, and increased incidences of goblet cell hyperplasia of the large intestine occurred in B6C3F1 mice. These results indicate that Aloe vera whole-leaf extract is an intestinal irritant in F344/N rats and B6C3F1 mice and a carcinogen of the large intestine in F344/N rats.

Protective Effect of <i>Aloe vera (Aloe barbadensis</i> Miller) on Erythrocytes Anion Transporter and Oxidative Change

The purpose of this study was to evaluate the ability of aqueous extract of Aloe barbadensis Miller (Aloe vera) on oxidative damage and Anion Exchanger 1 (AE1, also known as Band 3) expression in human erythrocytes exposed to the water soluble free radical initiator 2.2’-azobis-2-amidinopropano dihydrochloride (AAPH). In addition, total phenolic compounds in the extracts were determined as catechin equivalent and the various antioxidant activities were compared to natural and synthetic standard antioxidants such as BHA and ascorbic acid. Since Aloe vera extract did not cause a consumption of the cytosolic antioxidant, glutathione (GSH) when it was direct incubated with GSH in basic aerated aqueous solution, this indicates that Aloe vera extract does not proceed auto oxidation at this experimental condition. Furthermore, Aloe vera extract prevent the consumption of GSH, in radical treated RBCs. It also inhibit consumption of GSH when it was direct incubated with AAPH. Aloe vera gel extract inhibits the generation of diphenyl-2-picrylhy-drazyl (DPPH) and the scavenging activity was increased in a dose dependent manner. Aloe vera extract was shown the similar reducing power than standards BHT and ascorbic acid. Biochemical analysis by SDS-PAGE and western blotting showed that AAPH-induced oxidative stress increased the susceptibility of AE1 to proteolytic degradation. Of note, our data evidenced that Aloe vera treatment was able to partially restore the normal RBC membrane protein profiles in a dose-dependent manner. These results clearly demonstrate the antioxidative activity of Aloe vera gel extract that might be ascribed to a synergistic action of the bioactive compounds contained therein.

Isolation and identification of a phenolic antioxidant from Aloe barbadensis

A potent antioxidative compound has been isolated from a methanolic extract of Aloe barbadensis Miller using a combination of column and thin-layer chromatography. The antioxidant activity of this substance was similar to that of α-tocopherol as assessed in vitro using rat brain homogenates. On the basis of electrospray ionization and electron-impact ionization mass spectra in combination with reversed-phase, high-performance liquid chromatographic behavior, this compound has been identified as 8- C-β- d -[2- O-( E)-coumaroyl]glucopyranosyl-2-[2-hydroxy]propyl-7-methoxy-5-methylchromone.