Abstract
The purpose of this study was to evaluate the ability of aqueous extract of Aloe barbadensis Miller (Aloe vera) on oxidative damage and Anion Exchanger 1 (AE1, also known as Band 3) expression in human erythrocytes exposed to the water soluble free radical initiator 2.2’-azobis-2-amidinopropano dihydrochloride (AAPH). In addition, total phenolic compounds in the extracts were determined as catechin equivalent and the various antioxidant activities were compared to natural and synthetic standard antioxidants such as BHA and ascorbic acid. Since Aloe vera extract did not cause a consumption of the cytosolic antioxidant, glutathione (GSH) when it was direct incubated with GSH in basic aerated aqueous solution, this indicates that Aloe vera extract does not proceed auto oxidation at this experimental condition. Furthermore, Aloe vera extract prevent the consumption of GSH, in radical treated RBCs. It also inhibit consumption of GSH when it was direct incubated with AAPH. Aloe vera gel extract inhibits the generation of diphenyl-2-picrylhy-drazyl (DPPH) and the scavenging activity was increased in a dose dependent manner. Aloe vera extract was shown the similar reducing power than standards BHT and ascorbic acid. Biochemical analysis by SDS-PAGE and western blotting showed that AAPH-induced oxidative stress increased the susceptibility of AE1 to proteolytic degradation. Of note, our data evidenced that Aloe vera treatment was able to partially restore the normal RBC membrane protein profiles in a dose-dependent manner. These results clearly demonstrate the antioxidative activity of Aloe vera gel extract that might be ascribed to a synergistic action of the bioactive compounds contained therein.
Highlights
IntroductionAloe vera (L.) BURM. fil. (synonym A. barbadensis MILLER) (Liliaceae), is a perennial succulent plant belonging to the Aloeaceae family, a sub-family of the Asphodelaceae [1]
Aloe vera (L.) BURM. fil. (Liliaceae), is a perennial succulent plant belonging to the Aloeaceae family, a sub-family of the Asphodelaceae [1]
Since quantitative changes in this protein are important in maintaining cell morphology [11], we evaluated the Red Blood Cell Membrane (RBCM) modification as manifested by a decreasing cytoskeletal protein content, which can lead to abnormalities in RBC shape and disturbances in the microcirculation [12,13]
Summary
Aloe vera (L.) BURM. fil. (synonym A. barbadensis MILLER) (Liliaceae), is a perennial succulent plant belonging to the Aloeaceae family, a sub-family of the Asphodelaceae [1]. The Aloe vera gel could be useful to neutralize free radicals for maintaining the integrity of the antioxidant status According to this potential activity, the aim of this work was to study the properties of Aloe vera as radical scavenger protecting Red Blood Cell Membrane (RBCM) from 2,2,-Azobis (2-methylpropionamide) dihydrochloride (AAPH) induced oxidative injury and preserving the RBC Anion Exchanger 1 (AE1), called Band 3. This transporter is 95 kDa protein is composed of two functionally distinct domains.
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