Olive Oil Research Articles

BEFORE THE MIDDLE BRONZE AGE RE-URBANIZATION AT THE HINTERLAND SITE OF TOPRAKHİSAR HÖYÜK (HATAY, TÜRKİYE)

This article explores the Early Bronze Age IVB (ca. 2300-2100 BC) and Middle Bronze Age I (ca. 2100-1800 BC) levels encountered during the research-rescue excavations conducted at Toprakhisar Höyük, a peripheral site located in the hilly landscape of the Altınözü district of Hatay. The archaeological remains exposed provided the possibility to explore research questions regarding the Early Bronze Age collapse and Middle Bronze Age re-urbanization from the stand point of the highland periphery in relation to the city centers of Tell Tayinat and Tell Atchana in the Amuq Valley of Hatay. The mixed corpus of the ephemeral Early Bronze IVB assemblage that includes elements from the Early Transcaucasian to North Syrian cultural horizon was followed by the appearance of a new local material culture in the early phases of the Middle Bronze Age. This allows us to contribute to current debates, including the expansion of the Amorites and Hurrians, and brings forward a hypothesis developed around climate change induced long distance mobility patterns and cultural hybridization. The functional role of the site as an olive oil and wine producer has been suggested to be a strong economic aspect of the site in terms of its levels that witnessed environmental and anthropogenic stress followed by the subsequent urban expansion period.

A Review on the Formulation and Evaluation of Herbal Cold Creams Incorporating Natural Oils

Herbal cold creams, formulated with natural oils, have emerged as a popular choice in the cosmetic industry due to their natural composition, enhanced safety profile, and potential therapeutic benefits. These emulsions provide a cooling sensation upon application and offer advantages such as moisturizing, nourishing, and protecting the skin. The physiology of the skin, the mechanism of action of topical formulations, and the ideal properties of herbal cold creams play a crucial role in their formulation and effectiveness. Natural oils, such as neem, coconut, almond, olive, and sesame oil, enhance the therapeutic potential of cold creams by providing moisturizing, anti-inflammatory, antimicrobial, and anti-aging properties. Herbal cold creams can be formulated as oil-in-water (O/W) or water-in-oil (W/O) emulsions, each with its unique characteristics. The formulation process involves careful consideration of ingredient selection, emulsifier choice, preservation, and manufacturing methods. Evaluation parameters, including pH measurement, irritancy test, spreadability, viscosity, and dilution test, are essential to ensure the quality, stability, and efficacy of the formulated creams. The growing trend towards herbal cosmetics highlights the need for further research and development to create innovative, safe, and effective herbal cold cream formulations that cater to the diverse needs of consumers while adhering to regulatory requirements

The influence of high oleic oils during intermittent frying on the formation of acrylamide in french fries

High oleic oils are often used for industrial frying considering a better nutritional value and stability to thermal oxidation than high polyunsaturated fatty acid oils. This research aimed to investigate the correlation between total polar compound (TPC) and acrylamide content during intermittent frying using high oleic oils such as super palm olein (SPO), high-oleic canola oil (HOCO) and high-oleic sunflower oil (HOSO). All oils were accessed in terms of fatty acid composition and tocopherol quantity prior to intermittent frying. The TPC of frying oil was recorded at every 8th interval of the frying cycle and french fries fried with respective oils were collected, and oil was extracted for acrylamide quantification using HPLC-MS. Pearson correlation showed a positive correlation between the total polar compound of frying oil and the acrylamide content of french fries (p<0.01). SPO contained the highest total tocopherol content, followed by HOCO and HOSO, accordingly. French fries fried with SPO had the lowest acrylamide content, followed by HOCO and HOSO, respectively. By contrast, SPO had the highest TPC content throughout the frying cycles, followed by HOCO and HOSO. The presence of oleic acids might not produce higher TPC, however, it produced higher acrylamide formation in the french fries. Overall, SPO is suitable for intermittent frying uses as it yielded the lowest concentration of acrylamide in french fries.

Sexual dysfunction in dichlorvos-exposed male Wistar rat is ameliorated by curcumin and associated with the upregulation of testosterone.

Dichlorvos is an organophosphate pesticide that is commonly used for agricultural and domestic control of pests and insects. Despite its usefulness, it exerts reproductive toxicity and induces male sexual dysfunction. On the other hand, curcumin has been reported to improve sexual dysfunction. However, till date, no study has reported the impact of curcumin on dichlorvos-induced sexual dysfunction. This study investigated the effect and associated mechanism of curcumin on dichlorvos-induced sexual dysfunction. Thirty-two male Wistar rats were randomized into four groups; the control (1mL of olive oil), curcumin-treated (100mg/kg), DDVP-treated (98.54g/m3 of dichlorvos by inhalation), and DDVP + Curcumin-treated. Dichlorvos induced sexual dysfunction as depicted by reduced motivation to mate (8.38 ± 0.18 vs. 4.00 ± 0.33, P < 0.0001), prolonged latencies (46.63 ± 1.30 vs. 98.75 ± 1.32, P < 0.0001) and reduced frequencies of mount (14.88 ± 0.52 vs. 8.63 ± 0.38), intromission (9.38 ± 0.50 vs. 3.75 ± 0.31, P < 0.0001), and ejaculation (7.63 ± 0.38 vs. 1.50 ± 0.19, P < 0.0001). These findings were accompanied by suppression of hypothalamic-pituitary-testicular axis, evidenced by marked reductions in circulating FSH (60.00 ± 1.04 vs. 21.13 ± 0.52, P < 0.0001), LH (46.38 ± 1.38 vs. 19.00 ± 0.46, P < 0.0001), and testosterone (6.01 ± 0.50 vs. 0.74 ± 0.05, P < 0.0001). Nonetheless, the administration of curcumin in dichlorvos-exposed rats significantly attenuated dichlorvos-induced sexual dysfunction by improving the assessed indices of male sexual act. Also, curcumin significantly increased serum levels of FSH (21.13 ± 0.52 vs. 47.25 ± 0.10, P < 0.0001), LH (19.00 ± 0.46 vs. 43.00 ± 1.49), and testosterone (0.74 ± 0.05 vs. 3.98 ± 0.08, P < 0.0001). This study revealed that curcumin attenuated dichlorvos-induced sexual dysfunction by activating the hypothalamic-pituitary-testicular axis and upregulating circulating testosterone.

Comprehensive two-dimensional gas chromatography using a miniaturized multiloop splitter-based non-cryogenic artificial trapping (M-SNAT) modulation device for analysis of cannabis samples

Multiloop splitter-based non-cryogenic artificial trapping (M-SNAT) modulation technique was developed, miniaturized and applied in comprehensive two-dimensional gas chromatography (GC×GC) for analysis of cannabis samples. The approach employed deactivated fuse silica (DFS) columns configured into multiple loop splitter system halving the perimeters of the progressively upstream loops. This splitter device was located between the first (1D) semi-nonpolar column outlet and a microfluidic Deans switch (DS). Each splitter loop splits a peak into two subpeaks having the same area with different void times. Three loops were then applied resulting in the number of the split subpeaks (nsplit) of 8 for each peak, and retention time differences between any two adjacent subpeaks (∆tR,split) were the same. By applying periodic heartcut event (H/C) within every artificial modulation period (PAM) of nsplit×∆tR,split, comprehensive split-and-trapped modulation profiles of analytes could be selectively transferred onto the second (2D) polar column (30 m) without cryogen consumption. This artificial modulation system was applied for analysis of cannabis samples with enhanced 2D peak capacity (2nc∼15). The established method was applied to analyse cannabis extracts using vegetable oils with or without frying process. This reveals 454 different peaks with 76, 92, 35 and 70 specific components specifically observed by using olive oil extraction (OE), fried OE, coconut oil extraction (CE) and fried CE, respectively.

Mediterranean Diet gastronomic experiences using olive oil and goat cheese

The present study aims to develop three food formulations, spreadable olive oil, goat cream cheese, and a cold dessert made of carob and goat cheese, using local elements of the Mediterranean diet (MD), namely, olive oil, fresh goat cheese, and carob derivatives in addition to hydrocolloids. The final products, with the best spreadability, texture and consistency, were obtained using: a) methylcellulose 6% (w/v) for the spreadable olive oil; b) Locust Bean Gum (LBG) (0.5% w/w) for goat cream cheese; and c) Goat cream cheese (43.4%), carob flour (8.7%), glucose syrup (26.1%), olive oil (14.6%), powdered goat milk (3.8%), chia seeds (0.5%), LBG (0.7%), and cinnamon for the cold dessert. Subsequently, foods were refrigerated until evaluated by a consumer panel of 101 untrained tasters aged between 15 and 49 years. All the attributes were assessed on a scale from 1 to 5. The products were well accepted by the tasters with no significant differences registered among the different age groups regarding the food attributes, except for the sweetness of the dessert. The goat cream cheese obtained the highest acceptability (4.09 ± 1.16–3.88 ± 0.86) and creaminess (4.22 ± 0.80–4.33 ± 0.65) among the youngest consumers (<30 years), while the acceptability of spreadable olive oil was evaluated with values of 3.24 ± 1.33–3.17 ± 1.14 by the same age group, indicating the need to add seasonings, as salt substitutes, to improve the attributes flavour and aroma. The results highlight the potential of the three formulations to expand the range of food products and processing strategies in the hospitality, catering, and food industry, as well as to provide alternative foods associated with the MD food pattern.

Production of Lipopeptide Biosurfactant Using Wastewater from Parboiled Paddy Rice and Evaluation of Antifungal Property of the Biosurfactant Against Two Dermatophyte Fungi.

A previously isolated lipopeptide biosurfactant-producing bacterium Bacillus licheniformis SCV1 was investigated for the production of the biosurfactant using wastewater from parboiled paddy rice. The biosurfactant thus produced was evaluated for its antifungal property against dermatophyte fungi Trichophyton ajelloi and Microsporum fulvum. Results revealed that the bacterial strain reduced surface tension of the media from 56.16 ± 1 mN/m to 35 ± 0.9 mN/m within 12h, which further shrank to 29.3 ± 1 mN/m in 24h of incubation. The yield of the biosurfactant was 3.15 ± 0.25g/L at 48h of incubation. The obtained biosurfactant exhibited efficient emulsifying activity against a wide range of hydrophobic substrates such as crude oil, olive oil, engine oil, and kerosene oil used in the study. The critical micelle concentration of the biosurfactant was found to be 80mg/L. Structural characterization using FT-IR and TLC revealed that the biosurfactant produced by the strain in the wastewater is a lipopeptide consisting of surfactin and iturin. LCMS analysis revealed that the surfactin homologs range from C12 to C17-surfactin while the iturin contains C13 to C17-iturin homologs. It also revealed an in vitro study that the biosurfactant has antifungal properties against dermatophyte fungi Trichophyton ajelloi and Microsporum fulvum. Microscopic observation of the hyphae of the treated dermatophyte revealed disruption and fissure of the mycelia. The chemical composition of the wastewater revealed that it contains adequate nutritional composition and micronutrients to support bacterial growth. This is the first report that the wastewater of parboiled paddy could be used as a low-cost substrate for the production of lipopeptide biosurfactant, and the biosurfactant could be used for preventing dermatophytes fungi.

The Therapeutic Effect of Shirvan Herbal Ointment on Human Cutaneous Leishmaniasis Compared with Glucantime

Abstract Background: Cutaneous leishmaniasis (CL) infection is caused by the Leishmania major (L. major) parasite and affects 1.5 to 2 million people worldwide each year. Although research into vaccines and antiparasitic drugs has been somewhat successful, their adverse effects include high toxicity, prolonged regeneration, and scarring. This has highlighted the importance of research to replace natural products with antibacterial and antioxidant properties, such as vegetable extracts and oils. Since, the anti-leishmaniasis effect of each of the components of Shirvan herbal ointment (aloe vera, Brazembel, Nigella sativa, propolis, lavender, and olive oil) has been separately studied and confirmed, it seems that the combination of these components can have an increasing anti-leishmanial effect to treat CL. Therefore, this study investigated the therapeutic impact of Shirvan herbal ointment on Iranian patients with leishmaniasis in comparison with glucantime (meglumine). Materials and Methods: Sixty patients with leishmaniasis were divided into the control and test groups. The control and test groups received intralesional glucantime and Shirvan herbal cream (two times daily), respectively. Results: The size mean of the lesion was 51.5 ± 32.5 before and 11.11 ± 16.28 after treatment in the control group and 50.8 ± 31.2 before and 0.0 ± 0.0 after treatment in the test group. In addition, the period mean of treatment was 43.9 ± 14.4 days and 30.5 ± 7.4 days in the control and test groups, respectively. There was a significant difference in lesion size between the two groups after treatment. Conclusion: Data suggested that Shirvan herbal ointment can be an alternative drug in the treatment of human CL.

Effect of Aloe Vera, Olive Oil and Normal Saline on Episiotomy Pain and Healing among Primi-para Women

Effect of Aloe Vera, Olive Oil and Normal Saline on Episiotomy Pain and Healing among Primi-para Women

Evaluation of the Protective Effects of Vitamins E and D on Oxidative Stress and Inflammation Caused by Tamoxifen in the Renal Tissue of Female Wistar Rats

Background: Tamoxifen is an effective drug for breast cancer treatment and its side effects are the production of reactive oxygen species and kidney damage. As antioxidants, vitamins E and D may help decrease kidney dysfunction. Objectives: In the present study, the protective effects of vitamins E and D on renal toxicity caused by tamoxifen in female Wistar rats were investigated. Materials and Methods: Twenty-five adult female rats weighing 180-200 were randomly divided into five groups with 5 rats. Group C, T, TE, TD, and TED were treated with olive oil, tamoxifen, tamoxifen + vitamin E, tamoxifen +vitamin D, and tamoxifen + both vitamins for four weeks. ELISA Kits measured the oxidant and antioxidant tests and TNF-α in kidney tissue. The spectrophotometric method measured urea, uric acid, and creatinine in serum and urine. Results: Tamoxifen significantly decreased the weight of rats, GPx, CAT, SOD levels and increased TNF-α, urinary creatinine level and, serum uric acid, urea levels (P &lt; 0.05). But, treatment with vitamin D and simultaneous administration of vitamins led to a significant decrease in the level of (TNF-α) compared to the tamoxifen group (p &lt; 0.01). Also, the histopathology results showed that the simultaneous administration of vitamins has significantly resolved the damage caused by the use of tamoxifen. Conclusion: The present study's findings showed that using vitamins E and D prevents kidney damage through antioxidant and anti-inflammatory effects. In addition, using vitamins E and D probably showed stronger synergistic effects against kidney damage.

Enhanced hemocompatibility and antioxidant efficacy of chitosan-PCL nanofibrous mats with olive oil and honey nika cream for wound healing applications

This study introduces an innovative approach utilizing electrospun scaffold matrices comprising polycaprolactone (PCL) and chitosan (CS), enriched with active ingredients of olive and Honey Nika cream (NC). These matrices were assessed for their potential as advanced wound dressings. The microstructures shown uniformly smooth nanofibers with diameters ranging from 200 to 250 nm across various formulations. XRD results demonstrated that incorporating NC and CS simultaneously, increased the crystallinity of PCL. The thermal analysis illustrated that although the addition of NC and CS declined the thermal stability of PCL fibers, but had no significant influence on the microstructure of the PCL fibers, and scaffolds have suitable chemical stability. FTIR analysis revealed the presence of olive oil, honey, and propolis components in the scaffolds. The developed nanofibers demonstrated advantageous properties such as prolonged NC release due to the incorporation of chitosan, enhanced cell adhesion, optimal swelling and degradation rates, minimal hemolytic activity (less than 0.44%), and suitable antioxidant capabilities (approximately 50.97 for PNC mat). Further corroborated by in vitro cytocompatibility assessments via MTT assays were shown biocompatibility of the prepared nanofibrous mats. This research highlights the promise of PCL/CS electrospun nanofbrous scaffold for wound healing applications.

Antioxidant potential of Camellia tetracocca and meitan turquoise bud in inhibiting oxidative degradation of edible oils

Effective antioxidants are crucial for preventing lipid deterioration. This study aimed to investigate the antioxidant properties and inhibitory effect on lipid oxidation of Camellia tetracocca (CT) and Meitan Turquoise Bud (MTB) extracts. The research analyzed the total flavonoids, phenolics, and major components content of the extracts. Additionally, it evaluated the in vitro antioxidant ability (FRAP, DPPH, ABTS assays) and lipid peroxidation inhibitory activity, and calculated the binding energy of the major components and fatty acids. Compared to CT, MTB had higher total flavonoid and phenolic content, along with superior antioxidant activity in vitro. Both extracts effectively inhibited the increase of POV in camphor oil and olive oil containing 74.63% and 69.96% oleic acid, respectively. Interestingly, MTB outperformed CT twelve days earlier, but CT surpassed MTB twelve days later in inhibition rates on these oils. Molecular docking confirmed that the binding energy of the active ingredients in both extracts with oleic acid-type edible oil is higher than that with linoleic and linolenic acid types. These findings indicate that CT and MTB extracts are promising natural antioxidants for oleic acid-rich edible oils. Given their differing impact on the oxidation rate of edible oil, using both extracts together may produce superior outcomes.

The Effect of Giving Red Ginger Oil On Pain After Iud Installation at Midwifery Independent Practice in Turen Malang Regency

ABSTRACT IUD installation pain is caused by a foreign body that enters the uterus, so to help the adaptation process, it is necessary to have treatment both pharmacologically and non-pharmacologically. The more frequent the pain that arises, the more recommended non-pharmacological treatment because it has a low risk. In post-IUD pain, there is complementary care in the form of essential oils. Essential oils often used are olive oil, peppermint oil, lavender oil, coconut oil, clove oil, and the latest red ginger oil. Complementary care can be done by giving Red Ginger Oil as a natural analgesic. The purpose of this study is to examine the impact of administering red ginger oil on post-IUD discomfort in a midwifery practice in Turen, Malang Regency. Experimental quantitative research using a one-group pre- and post-test design is what this study employs. The 18 people that filled out the survey were chosen using a quota system. The findings of the research were obtained three days after the controlled intervention, after the administration of Red Ginger Oil. Supplemental care, which posed less of a threat, was easily comprehended by patients. This research found that red ginger oil greatly alleviates uterine discomfort when administered topically. Keywords: IUD, Red Ginger Oil, Pain

MicroRNAs that regulate PPAR levels are altered in the decidua of prepuberal offspring of diabetic rats: Prevention by maternal diets enriched in olive oil

MicroRNAs that regulate PPAR levels are altered in the decidua of prepuberal offspring of diabetic rats: Prevention by maternal diets enriched in olive oil

Gene cloning, IPTG-independent auto-induction and characterization of a novel hyperstable S9 prolyl oligopeptidase having lipolytic activity from Thermotoga naphthophila RKU-10T with applications

A hyperstable lipase from Thermotoga naphthophila (TnLip) was cloned and overexpressed as a soluble and active monomeric protein in an effectual mesophilic host system. Sequence study revealed that TnLip is a peptidase S9 prolyl oligopeptidase domain (acetyl esterase/lipase-like protein), belongs to alpha/beta (α/β)-hydrolase superfamily containing a well-conserved α/β-hydrolase fold and penta-peptide (GLSAG) motif. Various cultivation and induction strategies were applied to improve the heterologous expression and bacterial biomass, but TnLip intracellular activity was enhanced by 14.25- fold with IPTG-independent auto-induction approach after 16 h (26 °C, 150 rev min−1) incubation. Purified TnLip (35 kDa) showed peak activity at 85 °C in McIlvaine buffer (pH 7.0–8.0), and has great stability over a broad range of pH (5.0–10.0), and temperature (40–85 °C) for 8 h. TnLip exhibited prodigious resistance toward various commercial detergents, chemical additives, and salt. TnLip activity was improved by 170.51 %, 130.67 %, 127.42 %, 126.54 %, 126.61 %, 120.32 %, and 116.31 % with 50 % (v/v) of methanol, ethanol, n-butanol, isopropanol, acetone, glycerol, and acetic acid, respectively. Moreover, with 3.0 M of NaCl, and 10 mM of Ca2+, Mn2+, and Mg2+ TnLip activity was augmented by 210 %, 185.64 %, 152.03 %, and 116.26 %, respectively. TnLip has an affinity with various substrates (p-nitrophenyl ester and natural oils) but maximal hydrolytic activity was perceived with p-nitrophenyl palmitate (pNPP, 3600 U mg−1) and olive oil (1182.05 U mg−1). The values of Km (0.576 mM), Vmax (4216 μmol mg−1 min−1), VmaxKm−1 (7319.44 min−1), kcat (1106.74 s−1), and kcatKm−1 (1921.42 mM−1 s−1) were calculated using pNPP substrate. Additionally, TnLip degraded animals' fats and removed oil stains within 3 h and 5 min, respectively. All these features make halo-alkali-thermophilic TnLip as an auspicious contender for laundry detergents (cleaning bio-additive), fat degradation, wastewater treatment and endorse eco-friendly stewardship along with various other biotechnological applications.

Cucumeropsis mannii seed oil mitigates bisphenol-A-induced sperm and hormonal damages in F1-generation of F0-exposed male rats: An in-vivo and in-silico analysis

Exposure to endocrine-disrupting chemicals such as bisphenol A (BPA) has been linked to the sharp drop in male fertility observed worldwide. This study evaluates the protective benefits of Cucumeropsis mannii seed oil (CMSO) against BPA-induced transgenerational sperm indices and reproductive hormone disruptions in rats. Twenty-four F0-male rats (aged 4–5 weeks) were randomly assigned to six groups (n = 4 each) after CMSO characterization for flavonoids. Group A received 1 ml of olive oil. Group B received 100 mg/kg body weight (BW) of BPA whereas Group C had a BW of 7.5 ml/kg CMSO. Groups D, E, and F were administered 100 mg/kg_BW of BPA in addition to 7.5, 5.0, and 2.5 ml/kg_BW of CMSO, respectively. After two weeks of treatment, four mature female rats were introduced to each group and allowed to stay together with male rats for seven days to confirm mating. Thereafter, the pregnant female rats were separated from the male according to their groups and allowed to give birth after 21 days of gestation period. At six weeks, the F1-male rats were isolated from each group and sacrificed for biochemical analysis. In-silico, CMSO flavonoids were assessed for drug-likeness and oral absorbability, followed by molecular docking to study their androgen receptor targeting mechanisms. Results showed that total flavonoids were 17.3652 ± 8.85 g/100 g. In-vivo, BPA significantly (p < 0.005) dysregulated sperm indices and male fertility hormones in F1 rats, but co-administration with CMSO restored these dysregulations in F1 rats. In-silico, the druglike molecules exhibited stronger binding affinities (−6.2 to −8.5 kcal/mol) for the androgen receptor than BPA (−6.1 kcal/mol) within the binding pockets. We postulated that CMSO mitigates transgenerational sperm indices and male fertility hormone disruption in F1 male offspring following F0 male exposure to BPA perhaps by utilizing androgen receptor signaling

Novel extracellular lipase gene Lip1728 influences nutrient-dependent performance bacterial quorum sensing of Burkholderia pyrrocinia WZ10-3

Quorum sensing (QS) is a cellular communication mechanism in which bacteria secrete and recognize signaling molecules to regulate group behavior. Lipases provide energy for bacterial cell growth but it is unknown whether they influence nutrient-dependent QS by hydrolyzing substrate. A high-yield lipase-producing strain, Burkholderia pyrrocinia WZ10–3, was previously identified in our laboratory, but the composition of its crude enzymes was not elucidated. Here, we identified a key extracellular lipase, Lip1728, in WZ10–3, which accounts for 99 % of the extracellular lipase activity. Lip1728 prefers to hydrolyze triglycerides at sn-1,3 positions, with pNP-C16 being its optimal substrate. Lip1728 exhibited activity at pH 5.0–10.0 and regardless of the presence of metal ions. It had strong resistance to sodium dodecyl sulfate and short-chain alcohols and was activated by phenylmethanesulfonylfluoride (PMSF). Lip1728 knockout significantly affected lipid metabolism and biofilm formation in the presence of olive oil. Finally, oleic acid, a hydrolysate of Lip1728, influenced the production of the signal molecule N-acyl homoserine lactone (AHL) and biofilm formation by downregulating the AHL synthetase gene pyrI. In conclusion, Lip1728, as a key extracellular lipase in B. pyrrocinia WZ10–3, exhibits superior properties that make it suitable for biodiesel production and plays a crucial role in QS.

Comparative Transcriptomic and Lipidomic Analysis of Fatty Acid Accumulation in Three Camellia oleifera Varieties During Seed Maturing.

Camellia oleifera, a major woody oil crop in China, produces tea oil rich in unsaturated fatty acids, earning it names like liquid gold and eastern olive oil. This study provides an integrated investigation of the transcriptome and lipidome within seeds at the maturing process across three C. oleifera varieties, revealing a significant relationship between fatty acid production and genes involved in lipid synthesis. Through transcriptomic analysis, 26,344 genes with varied expression were found. Functional enrichment analysis highlighted that pathways related to starch and sucrose metabolism, plant hormone signal transduction, and lipid accumulation were highly enriched among the differentially expressed genes. Coordinated high expression of key genes (ACCase, KAS I, KAS II, KAS III, KAR, HAD, EAR, SAD, LPAAT, LACS, DGAT, PDAT) during the late maturation stage contributes largely to high oil content. Additionally, expression variations of SAD and FADs among different varieties were explored. The analysis suggests that high expression of genes such as FAD3, FAD7, and FAD8 notably increased linolenic acid content. This research provides new insights into the molecular mechanisms of oil biosynthesis in C. oleifera, offering valuable references for improving yield and quality.

Hepatoprotective activity of Mentha rotundifolia aqueous extract against hepatocellular damage induced by CCL4 in rats

This study aims to assess hepatoprotective properties of M. rotundifolia. Decoction was used to prepare the aqueous extract. The preliminary cytotoxicity evaluated against Caco 2 and RAW 264 cells demonstrate no cytotoxic effect. The preventive impact of the extract against liver damage was evaluated by examining blood levels of AST, ALT, ALP, total proteins, and histological alterations in liver tissues. Thirty albino rats were separated into five groups: the first served as normal group, the second was injected by olive oil (3 ml/kg), and the third was injected by CCL4 (3 ml/kg). However, groups IV and V received daily doses of 250 and 500 mg extract/kg bw, respectively before CCL4 injection. The results showed that the administration of the extract led to a marked improvement in plasma biochemical markers and a reduction in symptoms of CCL4-induced liver damage. The extract exhibits hepatoprotective activity, which may be attributed to its phytochemical components.

The effect of different edible oils on body weight: a systematic review and network meta-analysis of randomized controlled trials

BackgroundObesity is a major public health issue with no definitive treatment. The first-line approach for obesity is lifestyle modification, including a healthy diet. Although the amount of fat has been considered, there is no network meta-analysis (NMA) study investigating the effect of edible oils on body weight. Therefore, we sought to investigate the effect of different edible oils on body weight using a systematic review and NMA study of randomized controlled trials (RCTs).MethodPubMed, Scopus, ISI Web of Science, and the Cochrane Library were searched from inception to April 2019. RCTs of different edible oils for body weight were included. A frequentist network meta-analysis was conducted to appraise the efficacy of different types of edible oils, and the Surface Under the Cumulative Ranking Curve (SUCRA) was estimated. The GRADE framework was used to assess the certainty of evidence.ResultsForty-two eligible studies were included. Most of the included trials examined the effect of olive oil compared to canola oil (n = 7 studies), followed by canola oil compared to sunflower oil (n = 6 studies), and olive oil compared to sunflower oil (n = 4 studies). Sesame oil had the highest SUCRA value for reducing weight (SUCRA value = 0.9), followed by the mixture of canola and sesame oil (0.8). Palm oil and soy oil were ranked the lowest (SUCRA value = 0.2).ConclusionThere is low to moderate certainty of evidence showing that soybean, palm, and sunflower oils were associated with weight gain, while sesame oil produced beneficial anti-obesity effects.