AbstractAlameda County Water District has observed increased biofilter effluent manganese concentrations during winter operations. To investigate manganese removal across surface water biofilters during cold‐water conditions, trends were analyzed between water temperature and manganese removal across multiple testing scales, multiple biofilter influent water qualities, and both anthracite and GAC media. During acclimation of new biofilters, 100% removal of manganese was observed sooner across both anthracite and GAC biofilters brought online at 20°C compared to 12°C. Acclimation at 12°C required 18 extra days for the GAC biofilters and 48 additional days for the anthracite biofilters. For fully acclimated biofilters, a decrease in manganese removal across both GAC and anthracite biofilters at temperatures below 15°C was observed. However, greater and more consistent manganese removal was observed across GAC compared to anthracite biofilters. Performance differences between locations also suggest that operational and water quality conditions also likely affect manganese removal.