Objectives Metastasis of multiple regional lymph nodes and extracapsular spread in the metastatic nodes are found to be negative prognostic factors for oral squamous cell carcinomas (OSCCs), and many studies on the prediction of nodal metastasis according to the clinicopathologic and cellular/molecular features of cancer cells have been conducted. However, studies on the factors related to multiple nodal metastasis are not available. In this study, we carried out morphometric analysis of the nuclear polymorphism of cancer cells and determined its predictive value for multiple nodal metastases of OSCC. Study Design Twenty-six patients with OSCCs were included in this study. Of these 26 patients, 6 had no nodal metastasis, 7 had 1 or 2 metastatic nodes, and 13 had 3 or more metastatic nodes (up to 16 metastatic nodes per patient). Results First, we examined differences in the T category, histologic differentiation, and invasion pattern of primary tumors among the 3 groups (no metastasis vs 1–∼2 metastatic nodes vs ≥ 3 metastatic nodes). However, we could not find a clear relationship between the number of metastatic nodes and these clinicopathologic factors. Next, we determined whether or not the nuclear polymorphism of cancer cells was related to nodal metastasis. The area and perimeter of 50 nuclei in each tumor were measured by the image analysis software Image J. The nuclear polymorphism index was given by the formula: [(perimeter of nucleus)2 /(area of nucleus)]. The indices of nuclear polymorphism were 16.49 ± 0.79 in 6 tumors without metastasis; 16.46 ± 0.69 in 7 tumors with 1 to ∼2 nodal metastases; and 18.46 ± 1.29 in 13 tumors with 3 or more nodal metastases. The tumors with 3 or more nodal metastases showed a statistically higher value of nuclear polymorphism compared with the other 2 groups. Conclusions The results suggested that nuclear polymorphism be a predictive factor for multiple metastasis of cervical lymph nodes in OSCC.