Extinction Window Research Articles

The demise of the giant ape Gigantopithecus blacki.

The largest ever primate and one of the largest of the southeast Asian megafauna, Gigantopithecus blacki1, persisted in China from about 2.0 million years until the late middle Pleistocene when it became extinct2-4. Its demise is enigmatic considering that it was one of the few Asian great apes to go extinct in the last 2.6 million years, whereas others, including orangutan, survived until the present5. The cause of the disappearance of G. blacki remains unresolved but could shed light on primate resilience and the fate of megafauna in this region6. Here we applied three multidisciplinary analyses-timing, past environments and behaviour-to 22 caves in southern China. We used 157 radiometric ages from six dating techniques to establish a timeline for the demise of G. blacki. We show that from 2.3 million years ago the environment was a mosaic of forests and grasses, providing ideal conditions for thriving G. blacki populations. However, just before and during the extinction window between 295,000 and 215,000 years ago there was enhanced environmental variability from increased seasonality, which caused changes in plant communities and an increase in open forest environments. Although its close relative Pongo weidenreichi managed to adapt its dietary preferences and behaviour to this variability, G. blacki showed signs of chronic stress and dwindling populations. Ultimately its struggle to adapt led to the extinction of the greatest primate to ever inhabit the Earth.

Dual-Function Meta-Grating Based on Tunable Fano Resonance for Reflective Filter and Sensor Applications.

Localized surface plasmon resonance (LSPR)-based sensors exhibit enormous potential in the areas of medical diagnosis, food safety regulation and environmental monitoring. However, the broadband spectral lineshape of LSPR hampers the observation of wavelength shifts in sensing processes, thus preventing its widespread applications in sensors. Here, we describe an improved plasmonic sensor based on Fano resonances between LSPR and the Rayleigh anomaly (RA) in a metal-insulator-metal (MIM) meta-grating, which is composed of silver nanoshell array, an isolation grating mask and a continuous gold film. The MIM configuration offers more freedom to control the optical properties of LSPR, RA and the Fano resonance between them. Strong couplings between LSPR and RA formed a series of narrowband reflection peaks (with a linewidth of ~20 nm in full width at half maximum (FWHM) and a reflectivity nearing 100%) within an LSPR-based broadband extinction window in the experiment, making the meta-grating promising for applications of high-efficiency reflective filters. A Fano resonance that is well optimized between LSPR and RA by carefully adjusting the angles of incident light can switch such a nano-device to an improved biological/chemical sensor with a figure of merit (FOM) larger than 57 and capability of detecting the local refractive index changes caused by the bonding of target molecules on the surface of the nano-device. The figure of merit of the hybrid sensor in the detection of target molecules is 6 and 15 times higher than that of the simple RA- and LSPR-based sensors, respectively.

HST proper motions on the far side of the Galactic bar—data

ABSTRACT This is the third paper in a series that attempts to observe a clear signature of the Galactic bar/bulge using kinematic observations of the bulge stellar populations in low foreground extinction windows. We report on the detection of ∼100 000 new proper motions in four fields covering the far side of the Galactic bar/bulge, at negative longitudes. Our proper motions have been obtained using observations from the Advance Camera for Surveys (ACS), on board of the Hubble Space Telescope (HST), with a time-baseline of 8–9 years, which has produced accuracies better than 0.5 mas yr−1 for a significant fraction of the stellar populations with F814W < 23 mag. Interestingly, as shown in previous works, the Hess diagrams show a strikingly similar proper motion distribution to fields closer to the Galactic center and consistent with an old stellar population. The observed kinematics point to a significant bulge rotation, which seems to predominate even in fields as far as l ≃ −8°, and is also reflected in the changes of the velocity ellipsoid in the l, b plane as a function of distance.

Too hot for the devil? Did climate change cause the mid‐Holocene extinction of the Tasmanian devil Sarcophilus harrisii from mainland Australia?

The possible role of climate change in late Quaternary animal extinctions is hotly debated, yet few studies have investigated its direct effects on animal physiology to assess whether past climate changes might have had significant impacts on now‐extinct species. Here we test whether climate change could have imposed physiological stress on the Tasmanian devil Sarcophilus harrisii during the mid‐Holocene, when the species went extinct on mainland Australia. Physiological values for the devil were quantified using mechanistic niche models of energy and water requirements for thermoregulation, and soil‐moisture‐based indices of plant stress from drought to indirectly represent food and water availability. The spatial pervasiveness, extremity and frequency of physiological stresses were compared between a period of known climatic and presumed demographic stability (8000–6010 BP) and the extinction period (5000–3010 BP). We found no evidence of widespread negative effects of climate on physiological parameters for the devil on the mainland during its extinction window. This leaves cultural and demographic changes in the human population or competition from the dingo Canis dingo as the main contending hypotheses to explain mainland loss of the devil in the mid‐Holocene.

Late Quaternary megafaunal extinctions in India: How much do we know?

Understanding global patterns of late Quaternary megafaunal extinction is impeded by geographic variation in data quality and quantity. The magnitude, timing and drivers of megafaunal extinctions remain poorly understood for India, a region with a strong history of palaeontological research. We review available data for all putative extinct Indian megafaunal taxa with direct or indirect reported evidence of potential survival into the global “megafaunal extinction window” (from ∼50,000 years onwards). Assessment of late Quaternary megafaunal species richness is confused by multiple levels of taxonomic uncertainty, including the relationship of named Late Pleistocene taxa to extant taxa, and nomenclatural confusion over correct species names. There is sufficient evidence to recognise up to four genuine global megafaunal species-level extinctions in India during the Late Pleistocene: two proboscideans (Palaeoloxodon namadicus, Stegodon sp.), a hippopotamus (Hexaprotodon sp.), and possibly a horse (Equus namadicus). A fifth extinct megafaunal species, the Indian aurochs Bos [primigenius] namadicus, definitely persisted into the Holocene. Other Indian late Quaternary megafaunal species (Rhinoceros spp., Bubalus palaeindicus, Sus “palaeindicus”, Crocodylus palaeindicus) are likely to be synonyms of extant species. Reconstructing regional extinction dynamics is further impeded by chronological uncertainty; however, attempts to obtain new dates for vertebrate samples from six late Quaternary sites in five regions were unsuccessful. Accurate understanding of the dynamics of megafaunal extinctions in India will require robust taxonomic, chronological and palaeoecological data, and we encourage further investigation of the region’s rich late Quaternary record.

Mapping the stellar age of the Milky Way bulge with the VVV

Context. A detailed study of the Galactic bulge stellar population necessarily requires an accurate representation of the interstellar extinction, particularly toward the Galactic plane and center, where severe and differential reddening is expected to vary on sub-arcmin scales. Although recent infrared surveys have addressed this problem by providing extinction maps across the whole Galactic bulge area, dereddened color-magnitude diagrams near the plane and center appear systematically undercorrected, prompting the need for higher resolution. These undercorrections affect any stellar study sensitive to color (e.g., star formation history analyses via color-magnitude diagram fitting), either making them inaccurate or limiting them to small and relatively stable extinction windows where this value is low and better constrained. Aims. This study is aimed at providing a high-resolution (2 arcmin to ∼10 arcsec) color excess map for the VVV bulge area in J − Ks color. Methods. We used the MW-BULGE-PSFPHOT catalogs, sampling ∼300 deg2 across the Galactic bulge (|l| < 10° and −10° < b < 5°) to isolate a sample of red clump and red giant branch stars, for which we calculated the average J − Ks color in a fine spatial grid in (l, b) space. Results. We obtained an E(J − Ks) map spanning the VVV bulge area of roughly 300 deg2, with the equivalent of a resolution between ∼1 arcmin for bulge outskirts (l < 6°) to below 20 arcsec within the central |l| < 1°, and below 10 arcsec for the innermost area (|l| < 1° and |b| < 3°).

Co-treatment of piracetam with risperidone rescued extinction deficits in experimental paradigms of post-traumatic stress disorder by restoring the physiological alterations in cortex and hippocampus

Pharmacotherapy and cognitive behavioral therapy, both fail to treat post-traumatic stress disorder (PTSD) in a considerable number of populations. The persistence of traumatic memories and deficit in extinction contributes to the failure of exposure therapy in PTSD.With the objective to enhance the outcomes of exposure therapy by targeting the extinction window using pharmacological agents in PTSD, the present study was aimed to explore the effect of piracetam, risperidone and their combinations in experimentally-induced PTSD-like phenotype in rats. Male SD rats were exposed to single prolonged stress model (SPS) for induction of PTSD-like behavioral changes. Piracetam, risperidone and their combination were used as therapeutic interventions while sertraline was used as a standard treatment for 14 days along with extinction training. Induction of PTSD-like behaviors were assessed in behavioral tests such as fear conditioning, elevated plus maze, social interaction test, and the marble burying test. Neurotransmitters (dopamine and serotonin and their metabolites), BDNF, proinflammatory cytokines (TNF-α, IL-6), caspase-3, and markers for oxidative stress were assessed in the hippocampus and cortex while corticosterone and nitrite levels were estimated in plasma. Our result indicated that the SPS paradigm efficiently induced PTSD-like phenotype in rats. Risperidone and piracetam were found to be effective alone, while their high dose combination, produced potentiating effect in reversing the extinction deficit, behavioral alterations, altered cortical and hippocampal BDNF, IL-6, TNF-α, caspase-3, oxidative stress markers, and neurotransmitter levels. Plasma corticosterone and nitrite levels were also found to be reversed in the combination treated groups. Our preliminary study suggests that piracetam, risperidone and their combination restored the physiological cascades in cortex and hippocampus along with successful suppression of fear memory and a symptom cluster of PTSD-like phenotype in rats. Hence they could be used as an effective adjunct to enhance the outcome of exposure therapy for the management of PTSD.

A new near-IR window of low extinction in the Galactic plane

Aims. The windows of low extinction in the Milky Way (MW) plane are rare but important because they enable us to place structural constraints on the opposite side of the Galaxy, which has hither to been done rarely. Methods. We use the near-infrared (near-IR) images of the VISTA Variables in the Vía Láctea (VVV) Survey to build extinction maps and to identify low extinction windows towards the Southern Galactic plane. Here we report the discovery of VVV WIN 1713−3939, a very interesting window with relatively uniform and low extinction conveniently placed very close to the Galactic plane. Results. The new window of roughly 30 arcmin diameter is located at Galactic coordinates (l, b) = (347.4, −0.4) deg. We analyse the VVV near-IR colour-magnitude diagrams in this window. The mean total near-IR extinction and reddening values measured for this window are AKs = 0.46 and E(J − Ks) = 0.95. The red clump giants within the window show a bimodal magnitude distribution in the Ks band, with peaks at Ks = 14.1 and 14.8 mag, corresponding to mean distances of D = 11.0 ± 2.4 and 14.8 ± 3.6 kpc, respectively. We discuss the origin of these red clump overdensities within the context of the MW disk structure.

A new flexible discrete-time model for stable populations

We propose a new discrete dynamical system which provides a flexible model to fit population data. For different values of the three involved parameters, it can represent both globally persistent populations (compensatory or overcompensatory), and populations with Allee effects. In the most relevant cases of parameter values, there is a stable positive equilibrium, which is globally asymptotically stable in the persistent case. We study how population abundance depends on the parameters, and identify extinction windows between two saddle-node bifurcations.

Timing and causes of mid-Holocene mammoth extinction on St. Paul Island, Alaska

Relict woolly mammoth (Mammuthus primigenius) populations survived on several small Beringian islands for thousands of years after mainland populations went extinct. Here we present multiproxy paleoenvironmental records to investigate the timing, causes, and consequences of mammoth disappearance from St. Paul Island, Alaska. Five independent indicators of extinction show that mammoths survived on St. Paul until 5,600 ± 100 y ago. Vegetation composition remained stable during the extinction window, and there is no evidence of human presence on the island before 1787 CE, suggesting that these factors were not extinction drivers. Instead, the extinction coincided with declining freshwater resources and drier climates between 7,850 and 5,600 y ago, as inferred from sedimentary magnetic susceptibility, oxygen isotopes, and diatom and cladoceran assemblages in a sediment core from a freshwater lake on the island, and stable nitrogen isotopes from mammoth remains. Contrary to other extinction models for the St. Paul mammoth population, this evidence indicates that this mammoth population died out because of the synergistic effects of shrinking island area and freshwater scarcity caused by rising sea levels and regional climate change. Degradation of water quality by intensified mammoth activity around the lake likely exacerbated the situation. The St. Paul mammoth demise is now one of the best-dated prehistoric extinctions, highlighting freshwater limitation as an overlooked extinction driver and underscoring the vulnerability of small island populations to environmental change, even in the absence of human influence.

Extinction window of mean field branching annihilating random walk

We study a model of growing population that competes for resources. At each time step, all existing particles reproduce and the offspring randomly move to neighboring sites. Then at any site with more than one offspring, the particles are annihilated. This is a nonmonotone model, which makes the analysis more difficult. We consider the extinction window of this model in the finite mean-field case, where there are $n$ sites but movement is allowed to any site (the complete graph). We show that although the system survives for exponential time, the extinction window is logarithmic.

Megafauna extinction in South America: A new chronology for the Argentine Pampas

Abstract The megafauna extinction in South America was one of the most profound events, with the loss of 50 genera (~ 83%). Three orders disappeared (Notoungulata, Proboscidea, Litopterna), as did all large xenarthrans, but how this fits into global extinction is uncertain, mainly due to the lack of chronological resolution. In recent years there has been an increase in the number of radiocarbon dating at archeological and paleontological sites, but this information varies greatly from area to area in South America, and few data can be considered to constitute a taxon-date. The timing of the late Pleistocene extinction in the Pampas is poorly established. Most taxa only appear in the biostratigraphic context and many reported 14 C dates do not meet rigorous criteria for accepting dates, including reports suggesting survival of megafauna into the Holocene. In the present paper, we evaluate the published radiocarbon dates in the pampas and present 20 new radiocarbon dates for paleontological sites in order to establish a more accurate “extinction window” for the key taxa. These new dates are sufficiently robust to assess correspondences among last-appearance records of megafauna, first-appearance records of humans, and the Younger Dryas to Holocene climatic transition in the Argentine Pampas. These results highlight the need for greater effort in taxa selection for dating.

Extinction risk and eco-evolutionary dynamics in a variable environment with increasing frequency of extreme events.

One of the most dramatic consequences of climate change will be the intensification and increased frequency of extreme events. I used numerical simulations to understand and predict the consequences of directional trend (i.e. mean state) and increased variability of a climate variable (e.g. temperature), increased probability of occurrence of point extreme events (e.g. floods), selection pressure and effect size of mutations on a quantitative trait determining individual fitness, as well as the their effects on the population and genetic dynamics of a population of moderate size. The interaction among climate trend, variability and probability of point extremes had a minor effect on risk of extinction, time to extinction and distribution of the trait after accounting for their independent effects. The survival chances of a population strongly and linearly decreased with increasing strength of selection, as well as with increasing climate trend and variability. Mutation amplitude had no effects on extinction risk, time to extinction or genetic adaptation to the new climate. Climate trend and strength of selection largely determined the shift of the mean phenotype in the population. The extinction or persistence of the populations in an 'extinction window' of 10 years was well predicted by a simple model including mean population size and mean genetic variance over a 10-year time frame preceding the 'extinction window', although genetic variance had a smaller role than population size in predicting contemporary risk of extinction.

Rio survey of optical astrometric positions for 300 ICRF2 sources and the current optical/radio frame link status before Gaia★

We obtained improved optical positions for 300 ICRF2 sources - the Rio survey. We compared the Rio survey with 10 other selected optical astrometric surveys and studied the link between the Hipparcos Catalogue Reference Frame (HCRF) and the International Celestial Reference Frame, Second Realization (ICRF2). We investigated the possible causes for the observed non-coincidence between the optical and ICRF2 positions. The Rio survey positions were referred to the second version of the United States Naval Observatory CCD Astrograph Catalog (UCAC2), currently the best tested HCRF densification. The sources are between -90 degrees < delta < +30 degrees. We used two telescopes with suitable diameters and focal lengths to properly link the observed ICRF2 sources with the UCAC2, using intermediate brightness stars. We certified the astrometry done with many statistical tests. The average 'optical minus ICRF2' offsets and respective standard deviations in (alpha, delta) were -3 mas (41 mas) and +4 (45 mas). The Rio survey represents well the zero-point offset of the other surveys. The standard error of 3.5 mas found for the HCRF/ICRF2 link indicates an error excess that can be originated by a non-coincidence between the observed optical/VLBI positions. We thus discussed the influence of the errors from the UCAC2. Then, we searched for correlations with the source morphology, represented by structure indices defined in the radio and in the optical domain. Finally, we studied how the position offsets could originate from the perturbation of the optical point spread function (PSF) of the source's core, by a second source of flux. We found an analytical relation that describes the resulting centroid shift, as a function of the atmospheric seeing, the brightness ratio and the relative distance between the two contributing flux sources. Two scenarios, modelled by this relation, are discussed: an extinction window in the dust torus nearby the core, and a Galactic star near the line of sight.

CONSTRAINTS ON THE FORMATION OF THE GALACTIC BULGE FROM Na, Al, AND HEAVY-ELEMENT ABUNDANCES IN PLAUT's FIELD

We report chemical abundances of Na, Al, Zr, La, Nd, and Eu for 39 red giant branch (RGB) stars and 23 potential inner disk red clump stars located in Plaut-s low extinction window. We also measure lithium for a super Li-rich RGB star. The abundances were determined by spectrum synthesis of high resolution (R~25,000), high signal-to-noise (S/N~50-100 pixel-1) spectra obtained with the Blanco 4m telescope and Hydra multifiber spectrograph. For the bulge RGB stars, we find a general increase in the [Na/Fe] and [Na/Al] ratios with increasing metallicity, and a similar decrease in [La/Fe] and [Nd/Fe]. Additionally, the [Al/Fe] and [Eu/Fe] abundance trends almost identically follow those of the {\alpha}-elements, and the [Zr/Fe] ratios exhibit relatively little change with [Fe/H]. The consistently low [La/Eu] ratios of the RGB stars indicate that at least a majority of bulge stars formed rapidly (<1 Gyr) and before the main s-process could become a significant pollution source. In contrast, we find that the potential inner disk clump stars exhibit abundance patterns more similar to those of the thin and thick disks. Comparisons between the abundance trends at different bulge locations suggest that the inner and outer bulge formed on similar timescales. However, we find evidence of some abundance differences between the most metal-poor and metal-rich stars in various bulge fields. The data also indicate that the halo may have had a more significant impact on the outer bulge initial composition than the inner bulge composition. The [Na/Fe] and to a lesser extent [La/Fe] abundances further indicate that the metal-poor bulge, at least at ~1 kpc from the Galactic center, and thick disk may not share an identical chemistry.

3-dimensional kinematics in low foreground extinction windows of the Galactic bulge

The detailed structure of the Galactic bulge still remain uncertain. The strong difficulties of obtaining observations of stars in the Galactic bulge have hindered the acquisition of a kinematic representation for the inner kpc of the Milky Way. The observation of the 3-d kinematics in several low foreground extinction windows can solve this problem. We have developed a new technique, which combines precise stellar HST positions and proper motions with integral field spectroscopy, in order to obtain reliable 3-d stellar kinematics in crowded fields of the Galactic center. In addition, we present results using the new techniques for six fields in our project. A significant vertex deviation has been found in some of the fields in agreement with previous determinations. This result confirms the presence of a stellar bar in the Galactic bulge.

Aridity, faunal adaptations and Australian Late Pleistocene extinctions

The faunal extinctions of the Late Pleistocene saw the disappearance of a suite of giant marsupials, birds and reptiles from the Australian landscape. Attempts to explain these extinctions have invoked human activities and climate change as one or other of the primary drivers. Recently, the identification of some faunal species having adaptations to aridity has been forwarded as evidence that climate change could not have had a major role in the process. By default, humans must have been responsible. The notion of aridity and adaptations to aridity is examined in this paper with respect to the extant Red Kangaroo (Macropus rufus) and the extinct giant kangaroo Procoptodon goliah in particular. The few sites known that document intact Late Pleistocene faunal sequences are also considered in an attempt to unravel climatic versus human influences. A mounting body of evidence points to considerable climate change in the lead up to, during and after the period sometimes referred to as the ‘extinction window’ (between ∼50 and 40 ka). How many species were present at the time of human arrival remains unclear and there remains little evidence of a human-megafauna coexistence. The case for a primary human role in the extinction process is inferred rather than proven and further palaeoenvironmental studies are needed to clarify the role of climate change in this process.

Looking for the archaeological signature in Australian Megafaunal extinctions

The decline and disappearance of a range of giant marsupials, reptiles and birds from the Australian landscape during the last Glacial cycle continues to capture the imagination of both researchers and the general public. The events hinted at in the Australian fossil sequences appear to be mirrored on other continents through similar time periods, though the exact cause or sequence of extinctions remains largely unresolved. In recent years debate over megafaunal extinctions in Australia has been dominated by reports that humans were the primary drivers, leading some to assert that it is no longer a question of whether humans drove these extinctions, only how. Following the review of Wroe and Field, new reports have been published that claim support for a human-driven extinction (HDE). On closer scrutiny however, the key sites and samples lack firm contextual data and/or clear provenance. These studies fail to prove a key tenet of the HDE – that all, or even most now-extinct species were present at the time of human colonization of the continent. As compared with the easy assertion 5 years ago that this had been established, there are now more faunal species (c. 69% of total known to have become extinct) that cannot be placed within 50–100 ka of human arrival. There are still only two sites that demonstrate a coexistence of humans with some species of megafauna – Nombe Rockshelter in the PNG highlands and Cuddie Springs in the semi-arid southeast of Australia. If there is so little empirical evidence for coexistence or association of megafauna with humans, then support for an HDE through overhunting and ecosystem disruption is seriously compromised. Furthermore, the popularly cited “extinction window”, proposed as c. 51–39 ka when the HDE is argued to occur, is still only a theoretical construct. There is no clear evidence indicating that this period was particularly significant in terms of faunal loss. At present, the great majority of ‘Pleistocene’ sites remain poorly dated and the understanding of faunal turnover through this epoch is almost non-existent. Small datasets, poorly constructed hypotheses and assertive rhetoric are the prominent features of current discussions on a human role in megafaunal extinctions. Importantly, it is yet to be established whether the extinctions are an archaeological problem. The sparse fossil record known from Australia hampers a clear resolution as to how and when the megafauna disappeared, a situation likely to continue into the near future.

RADIAL DISTRIBUTION OF X-RAY POINT SOURCES NEAR THE GALACTIC CENTER

(Abridged) We present the LogN-LogS and spatial distributions of X-ray point sources in seven Galactic Bulge (GB) fields within 4 deg from the Galactic Center (GC). We compare the properties of 1159 X-ray point sources discovered in our deep (100 ks) Chandra observations of three low extinction Window fields near the GC with the X-ray sources in the other GB fields centered around Sgr B2, Sgr C, the Arches Cluster and Sgr A* using Chandra archival data. To reduce the systematic errors induced by the uncertain X-ray spectra of the sources coupled with field-and-distance dependent extinction, we classify the X-ray sources using quantile analysis and estimate their fluxes accordingly. The result indicates the GB X-ray population is highly concentrated at the center, more heavily than the stellar distribution models. We also compare the total X-ray and infrared surface brightness using the Chandra and Spitzer observations of the regions. The radial distribution of the total infrared surface brightness from the 3.6 band $\mu$m images appears to resemble the radial distribution of the X-ray point sources better than predicted by the stellar distribution models. Assuming a simple power law model for the X-ray spectra, the closer to the GC the intrinsically harder the X-ray spectra appear, but adding an iron emission line at 6.7 keV in the model allows the spectra of the GB X-ray sources to be largely consistent across the region. This implies that the majority of these GB X-ray sources can be of the same or similar type. Their X-ray luminosity and spectral properties support the idea that the most likely candidate is magnetic cataclysmic variables (CVs), primarily intermediate polars (IPs). Their observed number density is also consistent with the majority being IPs.

Historical Mammal Extinction on Christmas Island (Indian Ocean) Correlates with Introduced Infectious Disease

It is now widely accepted that novel infectious disease can be a leading cause of serious population decline and even outright extinction in some invertebrate and vertebrate groups (e.g., amphibians). In the case of mammals, however, there are still no well-corroborated instances of such diseases having caused or significantly contributed to the complete collapse of species. A case in point is the extinction of the endemic Christmas Island rat (Rattus macleari): although it has been argued that its disappearance ca. AD 1900 may have been partly or wholly caused by a pathogenic trypanosome carried by fleas hosted on recently-introduced black rats (Rattus rattus), no decisive evidence for this scenario has ever been adduced. Using ancient DNA methods on samples from museum specimens of these rodents collected during the extinction window (AD 1888–1908), we were able to resolve unambiguously sequence evidence of murid trypanosomes in both endemic and invasive rats. Importantly, endemic rats collected prior to the introduction of black rats were devoid of trypanosome signal. Hybridization between endemic and black rats was also previously hypothesized, but we found no evidence of this in examined specimens, and conclude that hybridization cannot account for the disappearance of the endemic species. This is the first molecular evidence for a pathogen emerging in a naïve mammal species immediately prior to its final collapse.