Extensive Process Research Articles

Effect of Different Types of Ossification of the Posterior Longitudinal Ligament on the Dynamic Biomechanical Response of the Spinal Cord: A Finite Element Analysis.

Ossification of the posterior longitudinal ligament (OPLL) has been identified as an important cause of cervical myelopathy. However, the biomechanical mechanism between the OPLL type and the clinical characteristics of myelopathy remains unclear. The aim of this study was to evaluate the effect of different types of OPLL on the dynamic biomechanical response of the spinal cord. A three-dimensional finite element model of the fluid-structure interaction of the cervical spine with spinal cord was established and validated. The spinal cord stress and strain, cervical range of motion (ROM) in different types of OPLL models were predicted during dynamic flexion and extension activity. Different types of OPLL models showed varying degrees of increase in stress and strain under the process of flexion and extension, and there was a surge toward the end of extension. Larger spinal cord stress was observed in segmental OPLL. For continuous and mixed types of OPLL, the adjacent segments of OPLL showed a dramatic increase in ROM, while the ROM of affected segments was limited. As a dynamic factor, flexion and extension of the cervical spine play an amplifying role in OPLL-related myelopathy, while appropriate spine motion is safe and permitted. Segmental OPLL patients are more concerned about the spinal cord injury induced by large stress, and patients with continuous OPLL should be noted to progressive injuries of adjacent level.

Acute cold stress leads to zebrafish ovarian dysfunction by regulating miRNA and mRNA

Temperature is a critical factor that regulates the reproduction processes in teleost. However, the gonadal response mechanism to cold stress in fish remains largely unknown. In the present study, female zebrafish were exposed to different extents of low temperatures at 18 °C and 10 °C for 48 h. The ovarian histology was remarkably damaged after cold stress exposure. Integrated analysis of miRNA-mRNA was used to investigate the ovarian response to acute cold stress. A large number of mRNAs and miRNAs were altered by cold stress, which are involved in extensive biological processes. It is indicated that the signal transduction of MAPK and Calcium signaling pathway is highly engaged in zebrafish ovary to adapt to cold stress. The immune system was dysregulated by cold stress while the ovarian autophagy was activated. Remarkably increased gene number related to reproductive functions was identified in the cold stress at 10 °C compared to the control. The cold stress-induced dysregulated reproductive genes include star, hsd3b1, hsd17b1, inha, insl3, amh, nanos1 and foxl2. Combined with the dysregulated insulin, IGF and progesterone signaling, it is suggested that cold stress affects ovarian function in multiple aspects, including oocyte meiosis, folliculogenesis, final maturation and ovarian maintenance. On the other hand, the ovarian miRNA-mRNA regulatory network response to cold stress was also constructed. Overall, our result revealed the ovarian response to cold stress in zebrafish and provided insight into the fish adaptation mechanism to acute temperature change.

Sulforaphane ameliorated podocyte injury according to regulation of the Nrf2/PINK1 pathway for mitophagy in diabetic kidney disease

Mitophagy, a mechanism of self-protection against oxidative stress, plays a critical role in podocyte injury caused by diabetic kidney disease (DKD). Sulforaphane (SFN), an isothiocyanate compound, is a potent antioxidant that affords protection against diabetes mellitus-mediated podocyte injury. However, its role and underlying mechanism in DKD especially in diabetic podocytopathy is not clearly defined. In the current study, we demonstrated SFN remarkably activated mitophagy in podocytes, restored urine albumin to creatinine ration, and prevented the glomerular hypertrophy and extensive foot process fusion in diabetic mice. Simultaneously, nephroprotective effects of SFN on kidney injury were abolished in podocyte-specific Nuclear factor erythroid 2-related factor 2 (Nrf2) conditional knockout mouse (cKO), indicating that SFN alleviating DM-induced podocyte injury dependent on Nrf2. In vitro study, supplement with SFN augmented the expression of PTEN induced kinase 1(PINK1) and mediated the activation of mitophagy in podocytes treated with high glucose. Further study revealed that SFN treatment enabled Nrf2 translocate into nuclear and bind to the specific site of PINK1 promoter, ultimately reinforcing the transcription of PINK1. Moreover, SFN failed to confer protection to podocytes treated with high glucose in presence of PINK1 knockdown. On the contrary, exogenous overexpression of PINK1 reversed mitochondrial abnormalities in Nrf2 cKO diabetic mice. In conclusion, SFN alleviated podocyte injury in DKD through activating Nrf2/PINK1 signaling pathway and balancing mitophagy, thus maintaining the mitochondrial homeostasis.

Effects of thermal treatment on the fracture behavior of rock-concrete bi-material specimens containing an interface crack

The study of the fracture characteristics of rock-concrete structure containing an interface crack under high temperature is vitally significant for the durability and stability assessment of concrete construction projects on rock foundations. Brazilian splitting tests were performed on rock-concrete cracked straight-through Brazilian disc (CSTBD) specimens after different heat treatment temperatures. The crack extension process of the specimen was analyzed using digital image correlation (DIC) technique. The effects of temperature, interface crack inclination and concrete strength on fracture characteristics were investigated. The results indicate that at high temperatures, free water and structural water escape from rock and concrete, and crystal structure of the mineral composition is altered, which induces the development of internal pores and microcracks in the material and weakens the mechanical properties. The specimen displays brittle fracture behavior at 25 °C-300 °C, and plastic fracture behavior at 500 °C-700 °C. The wing crack extension in concrete (weak material) occurs preferentially, and this phenomenon becomes more apparent with raising temperature. When α ≤ 45°, the length of crack extension along the interface increases with increasing temperature, while when α > 45°, the increase in temperature makes the crack initiation closer to the disc center. The fracture energy and the difference in fracture energy between varying inclinations decrease with raising temperature. Furthermore, temperature has an essential impact on fracture toughness. As temperature increases, the fracture toughness generally decreases, the difference in mixed fracture toughness between different inclination angles gradually increases, and the fracture propagation resistance weakens.

Methodology for developing a prediction model for the remaining fatigue life and residual strength of tidal turbine blades

As tidal energy nears commercial viability, the reliability and safety of a tidal energy device becomes more prevalent. A key aspect for determining their reliability and safety, along with reducing risk during operational deployment, is the structural integrity of tidal turbine blades. Therefore, a validated model for predicting the structural integrity of tidal turbine blades will aid in de-risking tidal energy technologies. In this study, a three-phase approach was used to formulate a strategy to predict the remaining fatigue life and residual strength of tidal turbine blades, over their operational lifespan. In Phase 1, the parameters influencing the structural properties of tidal turbine blades were identified based on the literature review, and the expertise in the field. Then, parameters were extensively studied and classified into four main impact groups, which include load conditions, design and manufacturing, degradation, and unexpected situations. Loading conditions on the blade are directly linked to hydrodynamic forces, maintenance, operating conditions, and corrosion effects. At the same time, these scenarios can vary with fluid-structure interactions, climate conditions, local site conditions, and maintenance and inspection schedules of the blades. The design and manufacturing category mainly represents the impact of the properties of composite materials, the geometry of the blade, and manufacturing process parameters. Similar to the other structures, tidal turbine blades are subject to deterioration and unexpected accidents during their service life, which significantly compromises the structural integrity of the blade. In Phase 2, a data management strategy was formulated related to identified four impact categories and investigated the possible methods of analysing the data. In this context, finite element analysis of composite tidal turbine blades was identified as the most appropriate tool to comprehensively examine collected data, prior to comparing the results to the field and laboratory-based test data. Mesh properties of the numerical models, test standards, instrumentation, and equipment used for field and laboratory-based structural testing of tidal turbine blades, as well as the accuracy of data acquisition systems, influence the comparison of these results. Finally, with the information gathered, as well as knowledge and experience in the field, a method for estimating the residual strength and remaining fatigue life of tidal turbines at each stage of their operation was formulated. The model will undergo a series of extensive validation processes using experimental testing datasets and will be used in the future to develop vulnerability curves related to the remaining structural life of the tidal turbine blades.

Calibrated Design of High Strength Composite Columns

AbstractWhat does the general design method, described in EN 1994‐1‐1, mean in practice for the construction industry? Even though this method requires additional efforts, it provides improved design accuracy. High strength and high slenderness concrete filled steel tube composite columns with a solid steel core inside have undoubtable benefits. However, application of such columns requires the use of a general method of design and special knowledge not fully covered by Eurocodes.In order to utilize the general design method for ATLANT® Strong composite columns, Peikko has conducted an extensive development program. During the program, multiple tests were conducted on materials and the columns itself. The test results were used to calibrate models of Finite Element Analysis (ABAQUS), which allowed to investigate the behaviour of those columns even more extensively. Eventually, the general design method was implemented and calibrated, allowing reliable and optimized design.The paper contains an overview of ATLANT® Strong composite columns behaviour, the extensive testing program and calibration process to reach the optimal design of such columns. Additionally practical specifics, challenges and benefits are discussed.

Expert elicitations of smallholder agroforestry practices in Seychelles: A SWOT-AHP analysis

Agroforestry can leverage the co-benefits of climate change adaptation and mitigation while conserving biodiversity and restoring degraded and deforested lands. The preference of relevant stakeholders regarding agroforestry practices enhances sustainable land management through strategic decision-making in Seychelles and other island states. A suitable approach for assessing stakeholders’ preferences of agroforestry is the implementation of the strengths, weaknesses, opportunities, and threats (SWOT) approach in combination with the analytic hierarchy process (AHP) method. The entry point of this study is an extensive literature review process, during which 28 SWOT factors were identified. These SWOT factors were deliberated on during a half-day workshop with agricultural experts who agreed on 20 SWOT factors that reflect the local realities of the Seychelles through a consensus approach. Using the SWOT-AHP approach, focus group discussions were conducted to examine the perceptions of researchers and extension workers about the adoption of agroforestry in Seychelles. The results indicated that the positive aspects of smallholder agroforestry outweigh the negative aspects. For example, increased agricultural production, control runoff and soil erosion receive the highest scores among the strength factors perceived by researchers and extension workers, respectively. The willingness of international organizations to fund agroforestry-related projects and the existence of native tree species on farmlands have the highest scores among the opportunity factors. The lack of education, information, and communication between the government and farmers, and the small land size and crop competition have the highest scores among the weakness factors. Lastly, change in government policies on land use has the highest score among the threat factors by researchers, whereas the most significant threat is climate change and variability for the extension workers. The provision for a thirty-year land lease agreement in the National Agroforestry Policy of Seychelles is viewed by both groups as an incentive that could potentially drive the adoption and acceptability of agroforestry. Furthermore, better coordination of various efforts to promote agroforestry and more substantial extension services for farmers, especially the role of technologies for optimal production on small plots of land, can enhance climate resilience in Seychelles and other small island developing states.

A place for biosimilars in the changing multiple sclerosis treatment landscape.

The treatment paradigm for multiple sclerosis (MS), particularly relapsing-remitting MS, is heavily reliant on biologic disease-modifying therapies (DMTs). However, the current cost of treatment acts as a significant barrier to access for patients. Over the next few years exclusivity periods for key biologic medicines used in MS are likely to end, opening the door for biosimilar medicines to enter the market. In this review, we discuss what biosimilar medicines are, and how the existing experience with biosimilar medicines across multiple therapy areas can inform the assimilation of biosimilar medicines into the MS treatment landscape in Europe and the US. There is currently a lack of knowledge and awareness around the distinctions and similarities between small molecules, non-biological complex drugs, and biological medicines, as well as the different categories of follow-on successor medicines. These include biosimilar medicines that offer a matching efficacy and safety profile to the reference biologic. Understanding and recognition of the stringency of the approval pathways required for drug categories such as biosimilars are key in building confidence in treatment outcomes. For example, biosimilar medicines are sometimes perceived only as 'copies' of their reference biologic despite undergoing an extensive approval process requiring that no clinically meaningful differences are observed between the biosimilar medicine and the reference medicine. For MS, introduction of biosimilar medicines in the future will enable more people with MS to receive effective treatment, and also expand access to biologic DMTs in MS. Experiences from the use of biosimilars in multiple therapy areas have shown us that this can result in cost-saving benefits for a healthcare system. Introduction of biosimilar medicines in other therapy areas has also demonstrated the importance of appropriate, accurate education and information for their successful integration into clinical practice. In order to realize optimized treatment outcomes in MS in coming years and to find the appropriate place for biosimilar medicines in the changing MS landscape, it is essential that clinicians and people with MS understand the fundamentals of biosimilars, their potential benefits and consistency of treatment provided by a biosimilar medicine, given the matching efficacy and safety profile to its reference medicine. As evidenced in other therapy areas, biosimilar medicines may reduce key barriers to access by providing a cost-effective alternative to the MS treatment arsenal, while providing the same treatment outcomes as reference biologics.

Data-driven product optimization capabilities to enhance sustainability and environmental compliance in a marine manufacturing context

This paper investigates concerns related to product data and digital data flow when aiming to automate company processes. Accurate data is necessary to create value by enabling improved decision-making in product development, including sustainability capabilities. The case analyzed is an engineer-to-order (ETO) company operating in a low-volume marine manufacturing context. A participatory research approach is used to study two projects that are part of the company’s digital business transformation, aiming to digitalize information and autogenerate downstream processes. Building on the strengths promised by digitalization requires precise and extensive product and process information. An important facilitation capability is to create a digital thread from design to finished product, including product documentation. This is necessary to establish capabilities both to autogenerate appropriate compliance reporting as part of the product development process and to conduct virtual testing and validation before the physical equipment is acquired, resulting in a manufacturing process that is ‘right first time’. In addition, data capabilities guide and enable sound-decision making for improved sustainable practices in the early phase of product development. It is found that the data quality required to utilize tools within the context of Industry 4.0 demands changes to existing product design practices and focus on the three pillars harmonization, integration and automation of data and systems.

Archival plans, alterations, and 3D laser scanning of Erik Gunnar Asplund’s Stockholm Public Library

Erik Gunnar Asplund’s Stockholm Public Library is considered an important building in the history of twentieth-century architecture, yet relatively few archival plans have been saved from the library’s extended design process from 1919–25 and its subsequent construction until opening in 1928. This work presents the first systematic review of the available, digitised archival plans from Sweden’s National Centre for Architecture and Design (ArkDes), and explains Asplund’s gradual design and development of the project. Subsequent alterations in the library are briefly summarised, followed by the results from an extensive 3D laser scanning process throughout and around the building as it undergoes a significant period of renovation and maintenance. The results from 3D laser scanning create the first comprehensive and detailed record of the building for supporting future research, teaching, and renovation work. This study emphasises the mutual benefits of combining historical and technological approaches, and conducting academic research in parallel with contemporary renovation projects of historical architecture.

Thermostable allergens in canned fish: Evaluating risks for fish allergy.

Major fish allergens, including parvalbumin (PV), are heat stable and can withstand extensive cooking processes. Thus, the management of fish allergy generally relies on complete avoidance. Fish-allergic patients may be advised to consume canned fish, as some fish-allergic individuals have reported tolerance to canned fish. However, the safety of consuming canned fish has not been evaluated with comprehensive immunological and molecular analysis of canned fish products. We characterized the in vitro immunoreactivity of serum obtained from fish-allergic subjects to canned fish. Seventeen canned fish products (salmon n = 8; tuna n = 7; sardine n = 2) were assessed for the content and integrity of PV using allergen-specific antibodies. Subsequently, the sIgE binding of five selected products was evaluated for individual fish-allergic patients (n = 53). Finally, sIgE-binding proteins were identified by mass spectrometry. The canned fish showed a markedly reduced PV content and binding to PV-specific antibodies compared with conventionally cooked fish. However, PV and other heat-stable fish allergens, including tropomyosin and collagen, still maintained their sIgE-binding capacity. Of 53 patients, 66% showed sIgE binding to canned fish proteins. The canned sardine contained proteins bound to sIgE from 51% of patients, followed by canned salmon (43%-45%) and tuna (8%-17%). PV was the major allergen in canned salmon and sardine. Tropomyosin and/or collagen also showed sIgE binding. We showed that canned fish products may not be safe for all fish-allergic patients. Canned fish products should only be considered into the diet of individuals with fish allergy, after detailed evaluation which may include in vitro diagnostics to various heat-stable fish allergens and food challenge conducted in suitable environments.

On thermodynamics and relaxation properties of eukaryotic cells

Mechanical properties of eukaryotic cells play an important role in the development of pathological processes. Currently, there are no complete models reflecting the full phenomenology of the mechanical behavior of the cell. Statistical thermodynamics methods have the potential to provide a framework for understanding the influence of structural properties on the stress-strain state of cells under external loading. In this paper, we develop a statistically based thermodynamic description of the eukaryotic cell to simulate the processes of cell contraction or extension as well as stress relaxation. In addition, it is proposed to use an order parameter to describe the orientation properties of the cell cytoskeleton and obtained a form of free energy, depending on this parameter, temperature and external stress. An orientation-viscoelastic body as a model of a representative cell volume has been proposed in this paper. Following the linear thermodynamics, the evolution equations describing the mechanical behavior of the representative cell volume have been derived. It was shown that the power law for the relaxation times conventionally introduced by the usage of the fractional derivatives in generalized Maxwell-Kelvin viscoelasticity of the cell cytoskeleton mechanical behavior follows from the orientation kinetics of the ensemble of actin filaments revealing the orientation ordering with the signs of the first kind criticality. The introduction of a relaxation spectrum is discussed, allowing one to improve the consistency of simulation results with atomic force microscopy data of living cells.

White asparagus stem proteins, from waste to interface stabilizer in food foams

White asparagus stems are a large waste stream that, despite having a high nutritional quality, is currently underutilized. Plant waste stream utilization is often based on extensive processing, which uses copious amounts of resources, and can negatively affect protein functionality. Hence, our study aimed to explore alternative processing routes and characterize the effect of asparagus extract composition on interfacial and foaming properties.Screw pressing followed by mild purification methods was used to obtain and purify protein extracts from white asparagus stems. Mild purification by filtration removed particles and purification by dialysis removed molecular impurities. Removal of particles and molecular impurities led to improved interfacial properties, as shown with drop tensiometry. The particles disturbed interfacial network formation and the presence of small impurities reduced network density and in-plane interactions. Extracts without particles and molecular impurities formed a dense interface with stiff solid-like behavior and strong in-plane interactions, which resulted in superior foam stability. Additionally, foamability was improved after filtration and/or purification, which could be related to the air bubble size and adsorption rate.In this study, we identified critical factors for interface and foam stabilizing properties of asparagus proteins, and we revealed the great potential of mildly processed asparagus stem extracts for application in food foams.

Synthesis and cis-trans kinetics of an azobenzene-based molecular switch for light-responsive silica surfaces

Light-responsive materials design is an attractive research field that stimulates demand for the synthesis of novel molecular photoswitches. The novel photoswitches should be capable of being attached to a desired surface in order to provide it with light-responsive properties. The switchback to its ground state is expected to be gradual, with the possibility of acceleration. This study presents a comprehensive description of a chemical route to a molecular photoswitch based on an azobenzene derivative with a trifluoromethoxy group and a long alkyl chain. The photoswitch is capable of modifying submicrometer silica particles and silicon wafers as models of curved and flat surfaces, respectively. The thermal relaxation times of the synthesized azobenzene were studied in three different solvents: ethanol, chloroform, and toluene. The results reveal a significantly delayed relaxation process lasting for several days. This observation is found to be related to the polarity of the solvents used. To characterize the extensive thermal relaxation process, a methodology including the use of white-light pulses to accelerate cis-trans isomerization is suggested. The white light spectrum used in pulses affects thermal relaxation but does not alter the relaxation time extrapolated to zero. The density of the photoswitch molecules attached to particles varies from 0.7 to 1.7 molecules/nm2 as the initial amount of azobenzene in the reaction increases. Surface-attached photoswitches demonstrate reversible cis-trans isomerization behavior when exposed to UV and white light irradiation. A 14° contact angle change is observed when flat surfaces modified with photoswitches are irradiated with UV light.

Transient receptor potential channel 3 in human liver and gallbladder – An investigation in body donors

Since the discovery of TRP proteins in 1969, during studies of the fruit fly Drosophila melanogaster, interest around them and the subfamily of TRPC channels has remained high. TRPC3 was able to be detected in a number of organs in rodents, such as rats and mice, and also in various human tissues. For the most part, these investigations were carried out using gene expression of TRPC3. Further work has already confirmed the relevance of TRPC3 in the context of neurodegenerative diseases, such as spinocerebellar ataxia, and carcinogenic entities, such as ovarian carcinoma. An association with TRPC3 has also been demonstrated for diseases that affect the liver. In order to confirm the expression of TRPC3 in the human liver, this study uses samples taken from eight (n = 8) fixated human body donors and analyzed with immunohistochemistry. In accordance with the macroscopic anatomy of the organs, six samples (n = 6) of liver tissue and three (n = 3) of gallbladder tissue were obtained. TRPC3 was clearly detected in all liver and gallbladder samples examined. Thus, it is not unlikely that TRPC3 plays a role in the extensive metabolic processes of the liver and could also serve as a target for pharmacological interventions in an imbalance of calcium homeostasis.

The Methods of Determining Temporal Direction Based on Asymmetric Information of the Optic Disc for Optimal Fovea Detection

Accurate localization of the fovea in fundus images is essential for diagnosing retinal diseases. Existing methods often require extensive data and complex processes to achieve high accuracy, posing challenges for practical implementation. In this paper, we propose an effective and efficient approach for fovea detection using simple image processing operations and a geometric approach based on the optic disc’s position. A key contribution of this study is the successful determination of the temporal direction by leveraging readable asymmetries related to the optic disc and its surroundings. We discuss three methods based on asymmetry conditions, including blood vessel distribution, cup disc inclination, and optic disc location ratio, for detecting the temporal direction. This enables precise determination of the optimal foveal region of interest. Through this optimized fovea region, fovea detection is achieved using straightforward morphological and image processing operations. Extensive testing on popular datasets (DRIVE, DiaretDB1, and Messidor) demonstrates outstanding accuracy of 99.04% and a rapid execution time of 0.251 s per image. The utilization of asymmetrical conditions for temporal direction detection provides a significant advantage, offering high accuracy and efficiency while competing with existing methods.

Motivational and emotional states in self-directed language learning: a longitudinal study

The current longitudinal case study investigated emotional and motivational states in a self-directed and mobile-assisted language learning environment. The participant of the study was a highly motivated language learner who used the Busuu application for a period of one year to learn German. Tracing the participant’s emotional and motivational journey using solicited diaries and chat-based interviews, the study revealed that the process of self-directed language learning is associated with the experience of a variety of positive and negative emotions. Joy, hope, and interest were the prevailing positive emotions that were linked to learning vocabulary; and frustration, anger, and feeling annoyed were the negative emotions in relation to learning grammar and pronunciation. In general, the study found a strong connection between positive emotions and persistence in self-directed language learning. The findings also pointed to some possible causes of high dropouts in application based language learning, as lack of enough resources for learning some language skills (such as grammar) initiated and implicated extensive processes of negative emotions. The study contributes to the growing body of knowledge in mobile-assisted language learning, and calls for further research regarding the role of emotions in technology-based and self-directed learning environments.

Chitosan Nanoparticle/Simvastatin for Experimental Maxillary Bony Defect Healing: A Histological and Histomorphometrical Study.

Biomaterials such as chitosan and simvastatin (Sim) have been introduced to accelerate the extensive and multicellular biological process of bone healing. The aim of this study was to evaluate the bone healing potential of chitosan and Sim, alone or combined. Forty-two male New Zealand rabbits were divided into three groups: chitosan nanoparticles (ChN), Sim and chitosan simvastatin nanoparticles (ChSimN). Two bony defects were created in the maxillary bone. The hole on the right side received one of the experimental materials, while the other side was assigned as the control and left to heal without any intervention. Bone specimens were collected at 2 and 4 weeks and then taken for histological and histomorphometrical analyses. The histological findings revealed that ChN possessed the highest number of osteoblasts and osteoclasts at weeks 2 and osteocytes after 4 weeks. There was a significant difference between the two healing periods regarding all bone parameters across all groups. ChN stood out as the only group that had a significant difference in the count of all bone cells between the two periods, thus having the best potential in promoting bone healing.

Regulatory changes associated with the head to trunk developmental transition

BackgroundDevelopment of vertebrate embryos is characterized by early formation of the anterior tissues followed by the sequential extension of the axis at their posterior end to build the trunk and tail structures, first by the activity of the primitive streak and then of the tail bud. Embryological, molecular and genetic data indicate that head and trunk development are significantly different, suggesting that the transition into the trunk formation stage involves major changes in regulatory gene networks.ResultsWe explored those regulatory changes by generating differential interaction networks and chromatin accessibility profiles from the posterior epiblast region of mouse embryos at embryonic day (E)7.5 and E8.5. We observed changes in various cell processes, including several signaling pathways, ubiquitination machinery, ion dynamics and metabolic processes involving lipids that could contribute to the functional switch in the progenitor region of the embryo. We further explored the functional impact of changes observed in Wnt signaling associated processes, revealing a switch in the functional relevance of Wnt molecule palmitoleoylation, essential during gastrulation but becoming differentially required for the control of axial extension and progenitor differentiation processes during trunk formation. We also found substantial changes in chromatin accessibility at the two developmental stages, mostly mapping to intergenic regions and presenting differential footprinting profiles to several key transcription factors, indicating a significant switch in the regulatory elements controlling head or trunk development. Those chromatin changes are largely independent of retinoic acid, despite the key role of this factor in the transition to trunk development. We also tested the functional relevance of potential enhancers identified in the accessibility assays that reproduced the expression profiles of genes involved in the transition. Deletion of these regions by genome editing had limited effect on the expression of those genes, suggesting the existence of redundant enhancers that guarantee robust expression patterns.ConclusionsThis work provides a global view of the regulatory changes controlling the switch into the axial extension phase of vertebrate embryonic development. It also revealed mechanisms by which the cellular context influences the activity of regulatory factors, channeling them to implement one of several possible biological outputs.

Mapping and Change Assessment of Captive Limestone Mining Areas Using Landsat-5/8 Images

Limestone is a non-metallic mineral extensively used in cement manufacturing and construction sector. Extensive mineral mining processes impact the environment. The study aims to map and evaluate the limestone mining area change at the Yerraguntla industrial zone in the YSR district of Andhra Pradesh, India. The normalized difference vegetation index (NDVI) and modified soil-adjusted vegetation index (MSAVI) are computed from the Landsat-5/8 images using Quantum GIS (QGIS) software. Experimental results show that the limestone mining area increases from 307 ha to 469.92 ha during 2005-2019. NDVI method is more effective than MSAVI in change assessment of limestone mining areas with overall accuracy of 87.75 % and 79.49 % and kappa coefficient of 0.89 and 0.62 respectively in 2019. The finding is compared with industry field survey reports (487.10 ha). This study contributes to the limestone mining industry management in developing a land-environmental management plan for the long-term sustainability of limestone mining.