Daylength Extension Research Articles

TWIN SISTER OF FT (TSF) Acts as a Floral Pathway Integrator Redundantly with FT

In Arabidopsis, several genetic pathways controlling the floral transition (flowering) are integrated at the transcriptional regulation of FT, LFY and SOC1. TSF is the closest homolog of FT in Arabidopsis. TSF expression was induced rapidly upon activation of CONSTANS (CO). The mRNA levels of TSF and FT showed similar patterns of diurnal oscillation and response to photoperiods: an evening peak, higher levels in long day (LD) than in short day (SD) conditions, and immediate up-regulation upon day-length extension. These observations suggest that TSF is a direct regulatory target of CO. tsf mutation delayed flowering in SD conditions and enhanced the phenotype of ft in both LD and SD conditions. TSF and FT also shared similar modes of regulation by FLC, an integrator of autonomous and vernalization pathways, and other factors such as EBS and PHYB. Consistently, TSF overexpression caused a precocious flowering phenotype independent of photoperiods or CO, or FLC. These observations suggest that TSF is a new member of the floral pathway integrators and promotes flowering largely redundantly with FT but makes a distinct contribution in SD conditions. TSF and FT seem to act independently of each other and of LFY, and partially upstream of SOC1. Interestingly, the expression patterns of TSF and FT in seedlings did not overlap, although both were expressed in the phloem tissues. Our work revealed additional complexity and spatial aspects of the regulatory network at the pathway integration level. We propose that the phloem is the site where multiple regulatory pathways are integrated at the transcriptional regulation of FT and TSF.

Effects of Environmental Controls on the Growth of Wasabi (Eutrema japonica (Miq.) Koidz.) in a Nutrient Solution Cultivation System

The effects of controlling minimum nocturnal ambient air temperature, day length and culture medium temperature on growth and yield were studied for the purpose of developing a year-round cultivation system for wasabi (Eutrema japonica (Miq.) Koidz.) . When the minimum night temperature was 5°C in winter, the growth of wasabi leaf was slow but recovered when the temperature rose to 8°C. Extension of day length by supplemental lighting was found to enhance growth recovery. Wasabi was planted at different times of the year to compare the effects of seasonal variation on stem growth. The mean daily growth medium temperature was maintained at 13.5-28.1°C throughout the year. These results revealed little difference in growth and stem pungency due to the season of planting, and stems grew to about 60 g within two years of cultivation. Year-round, stable nutrient solution cultivation of wasabi under environmental control may be an economically feasible alternative to conventional, labor-intensive cultivation systems.

The Effect of Interrupting Short Day Cycles with Day-length Extension on Floral Bud Initiation in Strawberry (Fragaria × ananasa)

Experiments were conducted to investigate the potential effect on floral bud initiation in strawberry (Fragaria × ananasa, cv. Chandler) by interrupting inductive short day cycles with a day-length extension treatment. Vegetative plants were exposed to 10-, 15-, or 20-day cycles of inductive short days in growth chambers. After receiving an inductive short day treatment plants were transferred to a greenhouse where they were exposed to non-inductive long days, which stimulated panicle elongation. Dissections of apical meristems immediately following each cycle of short days revealed that cycles of 20 days resulted in detectable floral bud formation. After 15 days in the greenhouse, all short day treatments had initiated floral buds. In the greenhouse, under long days, subsequent flowering in cohorts of plants which had previously received inductive short days showed a positive correlation between interruption of short days with day length extension and reduction in the number of floral buds initiated on earliest emerging panicles. These results suggest potential for manipulation of floral bud induction and potentially fruit size in Chandler, and perhaps other cultivars by interruption of a cycle of inductive short days with a day length extension treatment.

Influence of Photoselective Films and Growing Season on Stem Growth and Flowering of Six Plant Species

Growth chambers constructed of photoselective plastic films were used to investigate light quality effects on flowering and stem elongation of six flowering plant species under strongly inductive and weakly inductive photoperiods. Three films were used: a clear control film, a far red (FR) light absorbing (AFR) film and a red (R) light absorbing (AR) film. The AFR and AR films intercepted FR (700 to 800 nm) and R (600 to 700 nm) wavelengths with maximum interception at 730 and 690 nm, respectively. The phytochrome photoequilibrium estimates of transmitted light for the control, AFR, and AR films were 0.71, 0.77, and 0.67. The broad band R:FR ratios were 1.05, 1.51, and 0.77, respectively. The photosynthetic photon flux was adjusted with neutral density filters to provide similar light transmission among chambers. Zinnia elegans Jacq., Dendranthema×grandiflorum Kitam. (chrysanthemum), Cosmos bipinnatus Cav., and Petunia×hybrida Vilm.-Andr. plants grown under the AFR film were shorter than control plants. The AFR film had no effect on height of Antirrhinum majus L. (snapdragon) or Rosa×hybrida (miniature rose). Anthesis of zinnia, chrysanthemum, cosmos (short-day plants), and miniature rose (day-neutral plant) was not influenced by the AFR films. Anthesis of petunia and snapdragon (long-day plants) was delayed up to 13 days by AFR films under weakly inductive photoperiods. In petunia, initiation and development of floral structures were not affected by the AFR films during strongly inductive photoperiods. However, during weakly inductive photoperiods, initiation of the floral primordia was significantly delayed and overall development of the floral meristem was slower than control plants indicating that the AFR films could increase the production time if long-day plants were produced off-season. Daylength extension with electric light sources could overcome this delay in anthesis yet achieve the benefit of AFR films for height reduction without the use of chemical growth regulators.

Grain and floret number in response to photoperiod during stem elongation in fully and slightly vernalized wheats

The manipulation of photoperiod sensitivity during stem elongation (terminal spikelet initiation—anthesis) has been suggested as a means to increase wheat yield potential. Nevertheless, photoperiod sensitivity and the interaction with vernalization during that phase have not been previously explored under field conditions. Thus, a field study was carried out (i) to assess whether duration of stem elongation is sensitive to photoperiod experienced only during that phase, and if so (ii) to evaluate whether vernalization interacts with this photoperiod response and (iii) to determine if altering duration of stem elongation may influence wheat yield and its components. The experiment was a factorial combination of two vernalization treatments (15 (V 15) or 50 (V 50) days at 4±1 °C and 8 h photoperiod), two photoperiod regimes during stem elongation (natural daylength in the field (NP+0) and one daylength extension of 6 h (NP+6)) and three cultivars (Buck Manantial (BM), Eureka Ferrocarril Sur (EFS), and ProINTA Puntal (PP)). The stem elongation phase responded to the actual photoperiod experienced during that phase in the three cultivars, increasing its duration with shorter photoperiod. In the vernalization-sensitive cultivars EFS and PP, plants vernalized 50 days showed greater response to photoperiod than those vernalized 15 days. In the most photoperiod-sensitive cultivar (BM), as duration of stem elongation lengthened by exposure to less inductive photoperiod, a higher number of fertile florets at anthesis was achieved leading to an increased grain number and thereby to a higher wheat yield. These results reinforce the idea that a longer duration of stem elongation may offer an alternative avenue to improve yield potential in wheat.

Influence of day length and temperature on number of main stem leaves and time to flowering in lupin

SummaryA growth chamber experiment was carried out to investigate the influence of day length and temperature on the development of flowering in eight varieties of the three grain lupin species Lupinus albus (Wat and C3396), L. angustifolius (Gungurru, Polonez and W26) and L. luteus, (Juno, Radames and Teo). The plants were grown at two temperatures, 10°C and 18°C, in combination with five daylength regimes: 10, 14, 18, 24 h day at full light intensity and 10 h full light extended with 8 h low intensity light. Increased daylength decreased days from sowing to flowering in all varieties, but had little effect on thermal time to flowering in most varieties. However, C3396, W26 and Radames had a significantly longer thermal time to flowering at high, non‐vernalising temperature (18°C) at short daylengths. Low light intensity daylength extension did not significantly influence thermal time to flowering. For flower initiation, measured as number of leaves on the main stem three types of response were found. All varieties formed fewer leaves on the main stem at 10°C than at 18°C, although the two thermo‐neutral varieties of L. luteus, Juno and Teo, gave only a small response to temperature and daylength. In Polonez, Gungurru and Wat, low temperature decreased leaf number, but there was only a small response to changes in daylength. Three varieties, C3396, W26 and Radames, showed longer thermal time to flowering at 18°C with short daylengths. This could be explained by a greater number of main stem leaves formed at short daylength at non‐vernalising temperatures. Increased daylength decreased leaf number in these varieties, but never to a smaller number than for plants grown at 10°C. In these varieties, low intensity extension of the daylength had a similar (W26, Radames) or decreased (C3396) effect compared to full light extension. The hastening of time to flowering by long days could be separated into two effects: a high light energy effect hastened development by increasing the rate of leaf appearance in all varieties, while low light energy in thermo‐sensitive varieties was able to substitute for vernalisation by decreasing leaf number.

Vernalization and photoperiod responses in wheat pre-flowering reproductive phases

It has been established that photoperiod influences the rate of wheat development well beyond the end of the vegetative phase. Conversely, vernalization effects are still assumed to be mainly during the vegetative phase. The aim of the study was to assess the effects of different combinations of vernalization and photoperiods on the developmental characteristics of wheat pre-flowering reproductive phases under field conditions. For this purpose, three high yielding cultivars (ProINTA Puntal, ProINTA Super and Klein Pegaso) were subjected to two vernalization treatments (unvernalized (V 0) and vernalized during 56 days in a cool chamber (V 56)) and four photoperiod regimes. The photoperiod treatments consisted of the natural photoperiod of the season (NP+0) and daylength extensions over that of 2 (NP+2), 4 (NP+4), and 6 (NP+6) hours. Photoperiod influenced the duration of early (collar—first node detectable) and late (first node detectable—heading) pre-flowering reproductive phases, reducing their durations as photoperiod increased. When cultivars with strong vernalization response (ProINTA Puntal and ProINTA Super) were not vernalized, duration of the early and late pre-flowering reproductive phases increased and the rate of spikelet initiation decreased. Thus, when vernalization requirements were not satisfied, the spikelet initiation rate was even slower than the leaf initiation rate (both expressed in a thermal time base). Results showed significant interactions between photoperiod and vernalization (vegetative phase), or clear, though non-significant, trends to interactive effects (late pre-flowering reproductive phase). Thus, the length of vegetative and late pre-flowering reproductive phases changed in response to photoperiod depending on the level of satisfaction of the vernalization requirements. The relationship between the cumulative number of leaves on the main stem and thermal time fitted a bi-linear model whenever the final leaf number (LN f) was large, e.g. when photoperiod was not extended or vernalization requirements not satisfied. Thus, responses during the pre-flowering reproductive phases were accompanied by an increased phyllochron for leaves emerging after the first 7–8 leaves. This study demonstrated with field grown plants that the length of the late pre-flowering reproductive phase in wheat is sensitive to photoperiod and that the response to this environmental factor could be changed by the level of satisfaction of the vernalization requirements.

The effects of different maturation conditions on seed dormancy and germination ofCenchrus ciliaris

AbstractSeeds ofCenchrus ciliarisL. were produced under different hydro–photo–thermal environments with and without fertilizer. Dormancy loss of spikelets and extracted caryopses was tested during dry after-ripening at 40°C and 43% equilibrium relative humidity. Caryopses had higher initial germination and lost their dormancy faster than spikelets. Dormancy of both caryopses and spikelets generally decreased with an increase of maturation temperature and fertility, whereas dormancy increased if water stress was imposed during maturation. The latter effect was smaller when the mother plants were exposed to water stress after caryopses were fully formed than when water stress cycles were applied throughout maturation. Daylength extension (to 14 or 18 h d-1) by artificial light increased dormancy of both caryopses and spikelets. The effect of long days declined when plants were exposed to natural daylight for more than 10 h d-1. The after-ripening curves were consistent with the hypothesis that dormancy periods of individual seeds are normally distributed within each seed lot. Rates of loss of dormancy were quantified by the slopes of these curves. In a given experiment, these rates were identical for caryopses but not always for spikelets that matured in diverse environments. Even for caryopses, however, the slopes varied between experiments. Therefore, the results do not support the hypothesis that a dormancy model can be applied universally to all seed lots ofCenchrus ciliaris. Methods of predicting the period of after-ripening required to achieve desired levels of dormancy for reseeding degraded rangelands are discussed.

Lamp Type and Irradiance Level for Daylength Extensions Influence Flowering of Campanula carpatica `Blue Clips', Coreopsis grandiflora `Early Sunrise', and Coreopsis verticillata `Moonbeam'

The effectiveness of cool-white fluorescent, high-pressure sodium, incandescent, and metal halide lamps for inducing flowering through daylength extensions in Campanula carpatica Jacq. `Blue Clips', Coreopsis grandiflora Hogg ex Sweet `Early Sunrise', and Coreopsis verticillata L. `Moonbeam' was compared. Lighting was delivered as a 7-hour day extension with photosynthetic photon flux (PPF) ranging from 0.05 to 2.0 μmol·m-2·s-1 following a 9-hour natural daylength. Threshold irradiance values for flowering ranged from <0.05 to 0.4 μmol·m-2·s-1, depending on species. Saturation irradiance values for Campanula carpatica `Blue Clips' and C. grandiflora `Early Sunrise' were between 0.2 ± 0.2 and 0.7 ± 0.5 μmol·m-2·s-1, and did not differ between lamps. An irradiance of 1.0 μmol·m-2·s-1 from any lamp was adequate for flowering in Coreopsis verticillata `Moonbeam'. Time to flower at irradiances above the saturation points did not differ significantly between lamp types for all species tested. Campanula carpatica `Blue Clips' and Coreopsis grandiflora `Early Sunrise' plants had significantly longer stems under incandescent lamps than in any other treatment. Coreopsis verticillata `Moonbeam' plants grown under cool-white fluorescent lamps had stems ≈10% longer than those grown under high-pressure sodium or incandescent lamps.

Application of Photoperiodic Manipulation in Vegetative Specialty Floral Crop Propagation and Flowering

Photoperiod requirements are important for optimum flower development, decreasing production time, year-round flowering, and/or for increasing vegetative growth necessary in stock plant production. The photoperiodic responses were determined for 24 vegetatively propagated specialty floral crops. Each plant species was grown at 8-, 10-, 12-, 14-, and 16-h photoperiods. Photoperiods were provided by 8 h of sunlight, then pulling black cloth and providing daylength extension with incandescent bulbs. Data collected included time to flower, flower number, and vegetative characteristics. Evolvulus nuttallianus `Blue Daze', Heliotropium arborescens `Fragrant Delight', and Orthosiphon stamineus `Lavender' were facultative short-day plants with respect to flowering. Time to flower increased as photoperiod increased. Duranta repens `Blue', Verbena hybrid `Tapien Lavender', and Verbena peruviana `Trailing Katie' were facultative long day plants with respect to flowering. Days to visible bud and first open flower decreased as photoperiod increased. Argeranthemum frutescens `Sugar Baby', Scaevola aemula `Fancy Fan Falls', and Portulaca hybrid `Apricot' had increased flower number as photoperiod increased from 8- to 16-h, although time to first flower initiation was not affected. Abutilon hybrid `Apricot', Duranta repens `Blue', Evolvulus nuttallianus `Blue Daze', Lotus berthelotii `Parrot's Beak', Lysimachia nummularia `Aurea Creeping Golden', Rhodanthe anthemoides `Milkyway', and Scaevola aemula `Fancy Fan Falls' had increased vegetative growth as photoperiod increased. All other species studied were day-neutral with regard to flowering and vegetative parameters.

Growth of Rhododendron cultivars as affected by temperature and light

SummaryThe purpose of this study was to investigate the adaptation of four Rhododendron cultivars to contrasting light and temperature conditions. Two evergreen rhododendron cultivars and two deciduous azaleas were grown for 112 d under short day (14 h) and long day (20 h) photoperiods combined with temperatures of 15 and 24°C. Additionally, these cultivars were compared for daylength extension at 24°C/long day under two irradiation treatments (incandescent lamps and fluorescent tubular lamps). The number of flushes of growth increased with increasing photoperiod and temperature in both evergreen cultivars and in R. canadense; azalea #89132 made only one flush in all treatments. In the evergreen cultivars the number of leaves per shoot in the first flush did not differ significantly between treatments, indicating that this character was predetermined by conditions during bud development. The number of leaves in later flushes increased with increasing photoperiod and temperature. The elongation growth of most flushes was also enhanced by longer photoperiod and higher temperature. High irradiation during photoperiodic extension further enhanced the growth. Azalea #89132 made more flower buds under high than low irradiation. The two evergreen cultivars differed in their growth habit. ‘Pohjola’s Daughter’ tended to continue growth in long days or at very high temperatures, and is thus predicted to thrive best in a maritime or semi-maritime cool climate. ‘Helsinki University’ responded to short daylength by ceasing growth regardless of temperature, and could be expected to perform successfully also in continental climates at latitudes around 45° N. R. canadense seemed to do best in a cool climate, but azalea #89132 should in time acclimatize in all kinds of climates within the limits of this study.

電球形蛍光ランプによるキクの電照に関する実験研究

電球形蛍光ランプによるキクの電照に関する実験研究

Heliconia wagneriana Petersen is a Short-day Plant

Seasonal flowering behavior of Heliconia wagneriana Petersen was found to be caused by short daylengths (SD) using artificial short days (8 to 9 hours) and long days as daylength extension or night break lighting with incandescent lamps. The natural time for flower initiation was estimated to be mid- to late October (11 hours 40 minutes to 11 hours 20 minutes) in Hawaii, and 120 to 150 days were required from the onset of inductive SD to inflorescence emergence. The results may be used to manipulate flower availability for flower markets.

The function of phytochrome A

ABSTRACTKnowledge of the photoperceptive function of phytochrome A has improved substantially thanks to the availability of mutants lacking phytochrome A and transgenic plants transformed with the PHYA gene in sense or anti‐sense orientation. In imbibed seeds, phytochrome A mediates very‐low‐fluence responses. In etiolated seedlings, phytochrome A mediates very‐low‐fluence responses, high‐irradiance responses under continuous far‐red light, responsivity amplification to phytochrome B and red‐light enhancement of the phototropic response to blue light. In light‐grown seedings, phytochrome A modulates the extent of response to reductions in red/far‐red ratio perceived by phytochrome B, perceives daylength extensions and night interruptions affecting flowering, and perceives light treatments resetting endogenous rhythms. Under natural radiation these abilities are manifested during seed germination and seedling de‐etiolation under dense canopies or extremely low light fluences, and during early neighbour detection, but other processes await experimental evaluation. Phytochrome A affects growth and development throughout the whole life cycle of angiosperms.

Photoperiodic Responses of Garden Chrysanthemum

The objective was to study the flowering response of garden cultivars of Dendranthemum × grandiflorum (Ramat.) Kitamura to temperature and photoperiod. Fifteen garden mum cultivars were grown in ten temperature (18 and 24°C constant day and night greenhouse temperatures) and photoperiod (8, 10, 12, 14, and 16 h) combinations. Rooted cuttings were pinched above the fifth node and placed in the temperature/photoperiod treatments. When axillary shoots developed, all but one shoot was removed to produce a single stemmed plant. Photoperiods were provided by delivering 8 h sunlight, then pulling black cloth and providing daylength extension with incandescent bulbs. Days to visible bud, days to first bud color, days to flower, node number, and stem length were measured. By 11 weeks after the start of photoperiod treatments, no difference was measured in days to flower in the 8-, 10-, and 12-h photoperiods at 18°C. Days to flower increased as photoperiod increased from 12 to 14 h. At 18°C, five cultivars flowered in the 16-h photoperiod, while 10 cultivars developed crown buds, i.e., flower buds that initiated but had not developed. At 24°C, there was no difference in days to flower in the 8and 10-h photoperiod, while days to flower increased as photoperiod increased from 10to 12-h treatment. Cultivars formed crown buds but had not reached flowering in the 14and 16-h photoperiods at 24°C. Regardless of temperature, stem length increased as photoperiod increased above 10 h.

Photoperiodic Responses of Ten Alternative Hanging Basket Species

The photoperiodic responses were determined for the following species: Abutilon hybrid `Apricot', Diascia hybrid `Ruby Fields', Evolvulus glomeratus `Blue Daze', Orthosiphon stamineus `Lavender', Portulaca oleraceae `Apricot', Scaevola aemula `Fancy Fan Falls', Sutera cordata `Mauve Mist' and `Snowflake', Tabernamontana coronaria `Double', and Tibouchina `Spanish Shaw'. Each plant species was grown at 8-, 10-, 12-, 14-, and 16-h photoperiods. Photoperiods were provided by delivering 8 h of sunlight, then pulling black cloth and providing daylength extension with incandescent bulbs. Air temperatures were monitored under each black cloth. Data collected included time to flower, number of flowers, and vegetative characteristics. Diascia, Sutera `Mauve Mist' and `Snowflake', Tabernamontana, and Tibouchina were day neutral with regard to flowering; i.e., no difference in days to visible bud or days to anthesis in response to photoperiod was observed. Portulaca and Scaevola increased in bud and flower number as photoperiod increased from 8 to 16 h, performing similar to quantitative long-day plants. There was no difference in time to flower for Portulaca; however, 70% more flowers were produced under the 16-h photoperiod, compared to the 8-h photoperiod. Scaevola had 26% more flowers under the 16-h than 8-h photoperiod. Abutilon, Evolvulus,and Orthosiphon performed as quantitative short-day plants. Days to visible bud and days to anthesis increased as photoperiod increased for Evolvulus and Orthosiphon, and Abutilon had decreased flower number as photoperiod increased. Although Abutilon had no difference in time to flower, there was a 43% increase in flowers on plants under the 8-h photoperiod vs. 16-h photoperiod. Evolvulus set visible bud and reached anthesis 10 days earlier under 8-h photoperiod than 16-h. Orthosiphon reached visible bud 32 days earlier under an 8-h photoperiod than a 16-h photoperiod.

Photoperiodic Response of Nine Alternative Hanging-basket Species

The photoperiodic responses were determined for the following species: Bacopa speciosa `Snowflake', Bidens ferulifolium, Brachycome multifida `Crystal Falls', Helichrysum bracteatum'Golden Beauty', Lysimachia procumbens (Golden Globes), Pentas lanceolata `Starburst', Scaevola aemula `New Blue Wonder', Streptocarpella hybrid `Concord Blue', and Streptosolen jamesonii (Orange Browallia). Each plant species was grown at 8-, 10-, 12-, 14-, and 16-hour photoperiods. Photoperiods were provided by delivering 8 hours sunlight, then pulling black cloth and providing daylength extension with incandescent bulbs. Bacopa speciosa `Snowflake', Bidens ferulifolium, Brachycome multifida `Crystal Falls', Helichrysum bracteatum `Golden Beauty', Scaevola aemula `New Blue Wonder', and Streptocarpella hybrid `Blue Concord' were day neutral, i.e., no difference in days to visible bud or days to anthesis in response to photoperiod were observed. Pentas lanceolata `Starburst' and Lysimachia procumbens (Golden Globes) were quantitative long day plants, i.e., days to anthesis decreased as daylength increased. No difference in days to visible bud, number of lateral shoots, number of nodes, or internode length were observed for Pentas lanceolata `Starburst'; however, days to anthesis for 14- and 16-hour photoperiods occurred 9 days earlier than 8-hour photoperiods. Days to visible bud for Lysimachia procumbens (Golden Globes) occurred 7 days earlier and days to anthesis was 9 days earlier under 14- and 16-hour photoperiods than 8-hour photoperiods. By week 8, only one flower per plant developed in the 8-hour photoperiod while 11 flowers per plant developed in the 14-hour photoperiod. Streptosolen jamesonii (Orange Browallia) was a qualitative short day plant. There was no difference in the days to anthesis between 8- and 10-hour daylength, each averaging 36 days from start of photoperiod treatment. Plants under 12- to 16-hour photoperiods did not flower.

Identifying Yield-optimizing Environments for Two Cowpea Breeding Lines by Manipulating Photoperiod and Harvest Scenario

Photoperiod and harvest scenario of cowpea (Vigna unguiculata L. Walp) canopies were manipulated to optimize productivity for use in future controlled ecological life-support systems. Productivity was measured by edible yield rate (EYR:g m-2 day-1), shoot harvest index (SHI: g edible biomass [g total shoot dry weight]), and yield-efficiency rate (YER:g edible biomass m-2 day-1 per[g nonedible shoot dry weight]). Breeding lines 'IT84S-2246' (S-2246) and "IT82D-889' (D-889) were grown in a greenhouse under 8-, 12-, or 24-h photoperiods. S-2246 was short-day and D-889 was day-neutral for flowering. Under each photoperiod, cowpeas were harvested either for leaves only, seeds only, or leaves plus seeds (mixed harvest). Photoperiod did not affect EYR of either breeding line for any harvest scenario tested. Averaged over both breeding lines, seed harvest gave the highest EYR at 6.7 g m-2 day-1. The highest SHI (65%) and YER (94 mg m-2 day-1 g-1) were achieved for leaf-only harvest of D-889 under an 8-h photoperiod. For leaf-only harvest of S-2246, both SHI and YER increased with increasing photoperiod, but declined for seed-only and mixed harvests. However, photoperiod had no effect on SHI or YER for D-889 for any harvest scenario. A second experiment utilized the short-day cowpea breeding line 'IT89KD-288' (D-288) and the day-neutral breeding line 'IT87D-941-1' (D-941) to compare yield parameters using photoperiod extension under differing lamp types. This experiment confirmed the photoperiod responses of D-889 and S-2246 to a mixed-harvest scenario and indicated that daylength extension with higher irradiance from high pressure sodium lamps further suppressed EYR, SHI, and YER of the short-day breeding line D-288.

Effects of Temperature, Photoperiod, and Light Quality on Flowering in Several Herbaceous Perennial Species

The influence of low temperatures on Campanula carpatica `Blue Clips' and Lavandula angustifolia `Munstead' flowering was determined; plants were stored at 5C for several weeks and forced under 9-h photoperiods with a 4-h night interruption (NI). C. carpatica, L. angustifolia, and Asclepias tuberosa were forced under NI at five temperatures (15–27C) and time to flower under each treatment was calculated. Flower number and size were reduced at highest temperatures. The effectiveness of cool-white fluorescent (CWF), high-pressure sodium (HPS), incandescent (I), and metal halide (MH) lights in inducing flowering in C. carpatica and Coreopsis lanceolata `Early Sunrise' was compared. Lighting was delivered as a 7-h daylength extension with PPF ranging from 0.05–2.0 μmol·m–2·s–1. Minimum irradiances above which all C. carpatica flowered were approximately 0.14, 0.12, 0.1, and 0.17 μmol·m–2·s–1, respectively. C. lanceolata under CWF displayed irregular flowering throughout the range of intensities used. Under HPS and MH, minimum irradiances for 100% flowering were 0.37 and 1.0 μmol·m–2·s–1, respectively, with sporadic flowering at lower intensities. Under I light, all C. lanceolata exposed to 0.12 μmol·m–2·s–1 or more flowered.

Photoresponses of Light-Grown phyA Mutants of Arabidopsis (Phytochrome A Is Required for the Perception of Daylength Extensions).

Several aspects of the photophysiology of wild-type Arabidopsis thaliana seedlings were compared with those of a phytochrome A null mutant, phyA-1, and a mutant, fhy1, that is putatively involved in the transduction of light signals from phytochrome A. Although phyA seedlings display a near wild-type phenotype when grown in white light (W), they nevertheless display several photomorphogenic abnormalities. Thus, whereas the germination of wild-type and fhy1 seeds is almost fully promoted by a pulse of red light (R) or by continuous far-red light (FR), phyA seed germination is responsive only to R. Following growth under day/night cycles, but not under continuous W, the hypocotyls of light-grown phyA and fhy1 seedlings are more elongated than those of wild-type seedlings. For seedlings grown under low red/far-red (R/FR) ratio light conditions, phyA and fhy1 seedlings display a more marked promotion of hypocotyl elongation than wild-type seedlings. Similarly, seedlings that are doubly null for phytochrome A and phytochrome B(phyA phyB) also have more elongated hypocotyls under low R/FR ratio conditions than phyB seedlings. This indicates that phytochrome A action in light-grown seedlings is antagonistic to the action of phytochrome B. Although wild-type, fhy1, and phyA seedlings flower at essentially the same time under both short-day and long-day conditions, an obvious consequence of phytochrome A deficiency is a pronounced late flowering under conditions where a short day of 8 h of fluorescent W is extended by 8 h of low-fluence-rate incandescent light. The evidence thus indicates that phytochrome A plays a role in seed germination, in the control of elongation growth of light-grown seedlings, and in the perception of daylength.