Vitellogenin Gene Expression Research Articles

Ovarian Development of Female-Female Pairs in the Termite,Reticulitermes speratus

In the rhinotermitid termite Reticulitermes speratus (Kolbe) (Isoptera: Rhinotermitidae), facultative parthenogenesis is known to occur occasionally and females cooperate with other females to found the colony. To elucidate the ovarian development in these two females, incipient female-female colonies were established under laboratory conditions, and the process of colony development was observed at 0.5, 1.5, 2.5, 3.5, and 7.5 months (stages I–V, respectively) after colony foundation. Ovarian development, vitellogenin gene expression, and juvenile hormone (JH) titers were examined. A precise reproductive cycle in both females was observed, in which the oviposition rate was relatively higher during stages I and II, decreased during stages III and IV, and then increased again at stage V. JH III titer and vitellogenin gene expression changed in parallel throughout the reproductive cycle of these queens. Ovarian maturation and vitellogenesis were similar in both females in a female-female colony at all stages examined, suggesting that no conflicts existed for two females in terms of oviposition.

The association between nC60 and 17α-ethinylestradiol (EE2) decreases EE2 bioavailability in zebrafish and alters nanoaggregate characteristics

Manufactured nanoparticles (NPs) released into surface waters will associate with other substances and these interactions may affect environmental fate and bioavailability of NPs and the associated substances. We investigated the association between aqueous aggregates of C60 (nC60) and synthetic estrogen, 17α-ethinylestradiol (EE2), and considered nC60 physicochemistry and EE2 bioavailability (by measuring vitellogenin (vtg1A/B) gene expression) in zebrafish. Bioavailability of EE2 was reduced with increasing concentration of nC60 (P < 0.05), and bioavailability of EE2 decreased further after aging 28 d with nC60. Reduction in EE2 bioavailability was correlated with computed surface area of nC60, and reduced bioavailability of EE2 upon aging was consistent with absorption of EE2 within nC60 aggregates. Size and zeta potential of nC60 particles were affected by EE2 (1 μg/L) and also by aging (28 d) in aqueous phase. Results indicate that nC60 can reduce bioavailability of some substances and influence environmental fate and transport of associated substances.

No bioavailability of 17α-ethinylestradiol when associated with nC 60 aggregates during dietary exposure in adult male zebrafish ( Danio rerio)

The C 60 fullerene is a manufactured carbon nanoparticle (CNP) that could pose a risk to humans and other organisms after release into the environment. In surface waters, C 60 is likely to be present as aggregates of nC 60 and these aggregates can associate with other substances that are toxic. Our goal was to evaluate the association of a model contaminant [17α-ethinylestradiol (EE2)] with nC 60 and determine bioavailability of EE2 after accumulation by a filter feeding organism [Brine shrimp (BS) Artemia sp .] and subsequent dietary exposure in zebrafish. Aqueous suspensions of nC 60 were prepared (600 mg C 60/900 mL, 6-month water stirred method) with/without EE2 (1 μg/L) and BS were exposed to these preparations. Accumulation of nC 60 in gut of BS was assessed by light microscopy, and C 60 were extracted from BS and concentration analyzed by HPLC. Adult male zebrafish were fed (5 d) live BS according to the following treatments: BS (control); BS containing nC 60; BS containing nC 60 + EE2; or BS containing EE2. Liver was excised from exposed fish and total RNA was extracted for assessment of vitellogenin gene ( vtg1 A/B) expression. The vtg1 A/B was highly up-regulated in fish exposed to BS containing EE2, but expression of vtg1 A/B did not differ from controls in other treatments. The EE2 associated with nC 60 did not become bioavailable in zebrafish during passage through the intestinal tract of zebrafish. Results have implications on the effect of nC 60 on the bioavailability of co-contaminants in organisms during dietary exposure.

Effects of exposure water volume, depuration time, and feeding status on vitellogenin mRNA induction in male medaka ( Oryzias latipes) exposed to17 β-estradiol

Bioassays measuring the induction of vitellogenin gene expression in male fish are widely used for revealing estrogenic activity in water samples. Measuring induction of vitellogenin mRNA in males by means of RT-PCR analysis is a sensitive way to detect exposure to estrogenic chemicals. To date, little work has been done to examine variations in exposure conditions for assessing estrogenic activity in water samples using this model system. Here we report the results of experiments investigating the effects of volume of treatment water, time since removal from treatment water (depuration), and short-term food deprivation on vitellogenin mRNA induction in male Japanese medaka ( Oryzias latipes). Fish exposed to a single concentration of E 2 while volume was manipulated were found to have similar levels of vitellogenin mRNA, though more E 2 was present at larger volumes. Removal of fish from E 2-treated-water to clean water after exposures reduced vitellogenin levels in as little 24 h, however, the vitellogenin levels of the fish transferred to the clean water remained above those of the control fish for at least 72 h. Depriving fish of food for up to 72 h during exposure to E 2 did not significantly reduce vitellogenin induction. Together these results support the conclusion that real time RT-PCR measurement of vitellogenin in male fish can be used as a robust indicator of exposure to estrogenic contaminants in water.

Development of a method for identifying elevated vitellogenin gene expression in the Sydney Rock Oyster (Saccostrea glomerata) as an indicator of endocrine disruption on the Sunshine Coast

Ecological Management & RestorationVolume 11, Issue 2 p. 143-146 Development of a method for identifying elevated vitellogenin gene expression in the Sydney Rock Oyster (Saccostrea glomerata) as an indicator of endocrine disruption on the Sunshine Coast Kelli Anderson, Kelli AndersonSearch for more papers by this authorFiona Burnell, Fiona BurnellSearch for more papers by this authorAnne Roiko, Anne RoikoSearch for more papers by this authorMegan Andrew, Megan AndrewSearch for more papers by this authorWayne O’Connor, Wayne O’ConnorSearch for more papers by this authorAbigail Elizur, Abigail ElizurSearch for more papers by this author Kelli Anderson, Kelli AndersonSearch for more papers by this authorFiona Burnell, Fiona BurnellSearch for more papers by this authorAnne Roiko, Anne RoikoSearch for more papers by this authorMegan Andrew, Megan AndrewSearch for more papers by this authorWayne O’Connor, Wayne O’ConnorSearch for more papers by this authorAbigail Elizur, Abigail ElizurSearch for more papers by this author First published: 21 July 2010 https://doi.org/10.1111/j.1442-8903.2010.00534.xCitations: 9Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume11, Issue2August 2010Pages 143-146 RelatedInformation

Two female-specific DSX proteins are encoded by the sex-specific transcripts of dsx, and are required for female sexual differentiation in two wild silkmoth species, Antheraea assama and Antheraea mylitta (Lepidoptera, Saturniidae)

doublesex ( dsx) is the bottom most gene of the sex-determination cascade of Drosophila melanogaster. The pre-mRNA of dsx splices to produce male- and female-specific transcripts which code for the male- and female-specific proteins, respectively. dsx homologues have been characterized from different (many in Diptera, two in Hypmenoptera and only one in Lepidoptera) insect species. Sex-specific splice forms of dsx pre-mRNA in all these species code for one male- and one female-specific DSX proteins, which regulate the downstream target genes responsible for sex-specific characters. In the present study we have cloned and characterized the dsx homologues from two saturniid silkmoths, Antheraea assama and Antheraea mylitta. The divergence time between Saturniidae and Bombycidae to which the domesticated silkworm, Bombyx mori belongs is estimated to be around 160.9 MY. Interestingly, the dsx pre-mRNA of these wild silkmoths sex-specifically splices to generate multiple splice variants. On the basis of their open reading frame (ORF) and conceptual translation, two female-specific (DSX F1 and DSX F2) and one male-specific (DSX M) proteins could be inferred, in both the moths. Presence or absence of a 15 bp stretch within the ORF of the two groups of female-specific transcripts resulted in the production of two distinct female-specific DSX proteins. The sex-specific DSX proteins have common amino-terminal sequence but sex-specific carboxy termini. The two female-specific DSX proteins (DSX F1 and DSX F2) share common DNA binding domain (DM domain) and oligomerization domain (OD domain) and differ only at their extreme C-termini by 21aa. Functional analysis of dsx transcripts in A. assama by dsRNA mediated knock-down resulted in complete abolition of expression of vitellogenin and hexamerin genes, the direct targets of the DSX proteins, irregular differentiation of gonads, and drastic reduction in fecundity and hatchability. Together, these results suggest the involvement of both the female-specific DSX proteins in the process of female sexual differentiation. Further, conservation of the 4th exon sequence, especially the PESS sequence responsible for the sex-specific splicing of Bmdsx in the female-specific transcripts of Aadsx and Amydsx, indicated the existence of a common mechanism of sex-specific splicing of dsx homologues in silkmoths. To our knowledge this is the first report of existence of multiple splice forms of dsx pre-mRNA encoding two female-specific DSX proteins.

Induction of Nlvg and suppression of Nljhe gene expression in Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) adult females and males exposed to two insecticides

The brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a typical pest in which resurgence is induced by insecticides. In the past, studies of resurgence mechanisms have focused on the physiological and ecological aspects of insecticide-induced stimulation of fecundity in adult females. Juvenile hormone (JH) plays a key role in the regulation of growth, development, diapause and reproduction in insects. The fecundity of insects is mainly regulated by the synthesis of vitellin (Vt) and vitellogenin (Vg). The synthesis of vitellogenin, in turn, is regulated by JH. The present study investigated the effects of two insecticides, deltamethrin and triazophos, on the gene expression of vitellogenin ( Nlvg) and the juvenile hormone esterase ( Nljhe), which is associated with reproduction. Our findings show that the Nlvg mRNA expression in adult females before and after mating is significantly induced by insecticides. Deltamethrin caused Nlvg mRNA expression to be 0.6, 1.7 and 2.1 times the control level at one, two and three days before mating (DBM) after adult emergence, respectively, and 2.5, 4.1 and 5.2 times the control levels at one, two and three days after mating (DAM), respectively. Triazophos exposure induced Nlvg mRNA expression at levels that were 1.2, 2.4 and 2.7 times the control levels at one, two and three DBM, respectively, and 4.1, 5.4 and 6.0 times the control levels at one, two and three DAM, respectively. The transcript level of Nlvg mRNA was higher for triazophos than for deltamethrin both before and after mating. However, the expression of Nljhe mRNA in adult females before mating was significantly suppressed by insecticides. The two insecticides significantly induced Nljhe mRNA expression in females after mating, and this effect increased with increasing insecticide concentration, especially at one and two DAM. The JHIII titer in N. lugens adult females both before and after mating increased with insecticide concentration. The JHIII titer in N. lugens adult males increased before mating and decreased after mating compared to the controls, indicating that JHIII in adult males is transferred to adult females via mating. The JH titer and insecticide concentration could be fitted into a significant regression equation. The present findings provide valuable information for improving our understanding of the molecular and hormone regulative mechanisms of insecticide-induced stimulation of fecundity in N. lugens.

Molecular cloning of vitellogenin gene and mRNA expression by 17α-ethinylestradiol from slender bitterling

Indigenous aquatic population such as fish could be used as a successful test species for evaluating the ecological effects in aquatic environment. In the present study, vitellogenin (Vtg) from slender bitterling ( Acheilognathus yamatsutae), an indigenous aquatic species in Korea, was cloned and sequenced to determine if the Vtg gene possesses an important characteristic so as to act as a sensitive biomarker for estrogenic endocrine disrupting chemicals (EEDCs). The sbVtg cDNA is 5010 bp in length, containing a 4653 bp open reading frame, which encodes 1550 amino acid residues. The sbVtg cDNA was divided into lipovitellin heavy chain (LvH), phosvitin (Pv), lipovitellin light chain (LvL) as well as a β′-component (β′-c) domain, and belongs to VtgAo2. SbVtg has conserved important sequences for Vtg functions such as signal peptide, VtgR-binding region, and disulfide bond formation, all of which are consistent with those of other teleosts. In addition, the male slender bitterling aqueous exposed to 17α-ethinylestradiol (EE2, 12.5, 25, and 50 ng/L) produced a statistically significant and concentration-dependent increase in hepatic Vtg mRNA expression, which showed a similar pattern to biliary estrogenic activity, measured by ERE-reporter gene assay. Thus, this study clearly indicates that the induction of Vtg in slender bitterling might be a suitable biomarker in toxicological research of EEDCs.

Triclosan has endocrine‐disrupting effects in male western mosquitofish, Gambusia affinis

Triclosan (TCS) is an antibacterial agent used in a variety of personal care and industrial products. Triclosan and its environmentally transformed derivative, methyl-TCS, have been detected in waters receiving effluent from public wastewater treatment plants. Previous studies have demonstrated that TCS has the potential to act as an endocrine disruptor. The present study tested the hypothesis that TCS acts as an endocrine-disrupting agent in fish. Mature male western mosquitofish, Gambusia affinis, were exposed to TCS concentrations of 100, 200, and 350 nM (29.0, 57.9, and 101.3 microg/L) for 35 d by the static renewal method. Induction of the normally female-limited vitellogenin gene expression and reduction in sperm count were quantified as biomarkers of endocrine disruption. Vitellogenin mRNA expression was significantly elevated in the 350 nM TCS treatment. Sperm counts in the same treatment group were significantly decreased. The mean hepatosomatic index in the 350 nM treatment group was significantly increased. This study demonstrates that TCS has the potential to act as an endocrine disruptor in male mosquitofish.

Sex- and tissue-specific effects of waterborne estrogen on estrogen receptor subtypes and E2-mediated gene expression in the reproductive axis of goldfish

This research examined the gene expression profile of three goldfish estrogen receptor (ER) subtypes in multiple tissues in relation to mRNA levels of aromatase B and vitellogenin (VTG) following waterborne estrogen exposures. The protocol consisted of: i) adult male goldfish in late gonadal recrudescence exposed to 1 nM 17β-estradiol (E2); ii) adult male and female goldfish in early sexual regression exposed to 1 nM E2 for 3, 6, 12 and 24 h; and, iii) sexually mature, adult male goldfish exposed to 0.3 nM 17α-ethynylestradiol (EE2) for 24 h. Liver produced the most consistent response with up-regulation of ERα in sexually regressed, mature and recrudescing males and in sexually regressed females. The dose and length of exposure, reproductive state and sex affected the auto-regulation of ERβ1 by E2. ERβ2 was not affected in any experiments suggesting it may not be auto-regulated by E2. Aromatase B and VTG gene expression were affected by E2, but also by other experimental conditions. EE2 induced liver ERα and VTG mRNA levels indicating that high environmental EE2 levels induce E2-mediated gene expression in a model teleost. These studies reveal a more complicated action of estrogenic compounds that has important implications on estrogenic endocrine disruptors in teleosts.

Molecular characterization and expression of vitellogenin ( Vg) genes from the cyclopoid copepod, Paracyclopina nana exposed to heavy metals

Induction of vitellogenin ( Vg) has been used as a biomarker of exposure to heavy metals and endocrine-disruption chemicals (EDCs) in aquatic organisms. Here, we identified the full-length Vg1 and Vg2 sequence from the brackish water copepod, Paracyclopina nana. Vg1 gene contained 5718 bp of the open reading frame (ORF) that encoded the putative protein of 1905 amino acids residues, while Vg2 gene consisted of 5442 bp of ORF, encoding the putative protein of 1813 amino acids residues. P. nana Vgs showed highly conserved domains in the N-terminal region. The phylogenetic analysis revealed that P. nana Vgs are distinct from other arthropods, such as insects and decapods, as it formed a clade with other copepods, Tigriopus japonicus and salmon louse ( Lepeophtheirus salmonis). The expression of Vg transcripts was detectable after the copepodid stages 4–5. Female copepods expressed over 83 times and 223 times more Vg1 and Vg2 transcripts, respectively, than males. When copepods were exposed to heavy metals (0.1 mg/L Cd, 0.4 mg/L Cu, and 2 mg/L AsIII) for 24, 48, 72, and 96 h, P. nana Vg transcripts were highly induced in a time-dependent manner. Interestingly, Vg2 gene was more susceptible than Vg1 to trace heavy metal exposure. This finding indicates that P. nana Vgs provide a potential indicator for assessing the toxic effect of heavy metals. In addition, we suggest P. nana as a potential model species for risk-assessment to environmental pollutants in brackish water.

Effects of Shortened Photoperiod on Gonadotropin-Releasing Hormone, Gonadotropin, and Vitellogenin Gene Expression Associated with Ovarian Maturation in Rainbow Trout

Reproductive activities of salmonids are synchronized by changes in photoperiod, which control the endocrine system via the brain-pituitary-gonadal axis. Gonadotropin-releasing hormone (GnRH) in the brain regulates synthesis and release of the pituitary gonadotropins (GTHs; FSH and LH). FSH and LH in turn stimulate the production of sex steroids for oocyte growth and maturation-Inducing steroid hormones for oocyte maturation and ovulation, respectively, in female salmonids. To clarify effects of long-term photoperiod manipulations on the reproductive activity of salmonids from early recrudescence to ovulation, we Investigated the gene expression profiles of GnRH, GTHs, and vitellogenin (VTG), and plasma sex steroids in female rainbow trout (Oncorhynchus mykiss). In addition, the percentages of eyed embryos and hatched alevins were examined together with the number of ovulated eggs to evaluate the effects of photoperiod regimes on egg quality. During late summer, the mRNA levels of GnRHs, GTHalpha, and LHbeta, and the plasma level of a maturational steroid (17alpha,20beta-dihydroxy-4-pregnen-3-one; 17,20beta-P) were significantly elevated by a gradually shortened photoperiod under constant temperature, in accordance with accelerated sexual maturation. The percentages of eyed embryos and hatched alevins from fish ovulated in August were comparable to those of control fish observed in December. These results clearly indicate that syntheses of GnRHs, LH, VTG, and 17,20beta-P are effectively accelerated by a programmed long-short photoperiod regime in early recrudescent female rainbow trout, without a marked deterioration in egg quality.

Pulp and paper mill effluents induce distinct gene expression changes linked to androgenic and estrogenic responses in the fathead minnow (Pimephales promelas)

Although effluent treatment systems within pulp and paper mills remove many toxicants and improve wastewater quality, there is a need to understand and quantify the effectiveness of the treatment process. At a combined news and kraft pulp and paper mill in northwestern Ontario, Canada, fathead minnow (FHM) reproduction and physiology were examined before, during, and after a short-term (6-d) exposure to 10% (v/v) untreated kraft mill effluent (UTK), 25% (v/v) secondary treated kraft mill effluent (TK), and 100% (v/v) combined mill outfall (CMO). Although UTK exposure significantly decreased egg production, neither TK nor CMO caused any reproductive changes. The expression of six genes responsive to endocrine-disrupting compounds, stress, or metals was then examined in livers of these fish using real-time polymerase chain reaction. In female FHMs, none of the three effluents induced significant expression changes in any genes investigated. By contrast, in males there were significant increases in the mRNA levels of androgen receptor, estrogen receptor (ER) beta, and cytochrome P4501A (CYP1A) upon UTK and TK exposure but no changes in ERalpha or vitellogenin (VTG) gene expression, whereas CMO exposure significantly increased the mRNA levels of ERalpha, VTG, and CYP1A. Together, these results suggest that kraft effluent before and after biological treatment contained compounds able to induce androgenic effects in FHMs, and that combination of kraft and newsmill effluents eliminated the androgenic compounds while inducing distinct and significant patterns of gene expression changes that were likely due to estrogenic compounds produced by the newsmill.

Juvenile Hormone titre and vitellogenin gene expression related to ovarian development in primary reproductives compared with nymphs and nymphoid reproductives of the termiteReticulitermes speratus

To elucidate the reproductive cycle of termite queens, incipient colonies of Reticulitemes speratus (Isoptera: Rhinotermitidae) are established under laboratory conditions, and the transition of colony development is observed at 0.5, 1.5, 2.5, 3.5, and 7.5 months (stages I–V, respectively) after colony foundation. Ovarian development, vitellogenin gene expression and Juvenile Hormone (JH) titres are examined in the queens and in nonphysogastric nymphoids collected from natural colonies. A reproductive cycle in queens is observed, in which the oviposition rate is relatively higher during stages I and II, and then decreases during stages III and IV. Vitellogenic oocytes are not observed in the ovaries during stages III and IV, and the expression level of the vitellogenin gene is low, suggesting that egg production in queens is repressed during these stages. However, vitellogenin gene expression and egg deposition in queens resumes during stage V. Juvenile Hormone levels rise during the transition from nymphs to stage I queens, and elevated JH titres are observed also during stages III and IV. The decrease in JH titre in queens at stage II precedes the decline in vitellogenesis at stages III and IV. Thus, JH titre and vitellogenesis are correlated in an offset pattern. However, nonphysogastric nymphoid reproductives do not have vitellogenic oocytes in their ovaries, and their JH titre is two-fold higher than that of queens, suggesting that elevated JH titre precedes vitellogenesis, as in queens.

The effects of farnesoic acid and 20-hydroxyecdysone on vitellogenin gene expression in the lobster, Homarus americanus, and possible roles in the reproductive process

Reproduction in female lobster ( Homarus americanus) is characterized by the maturation of the ovary, with a gradual increase in its size as a result of uptake of yolk protein precursor, vitellogenin (Vg) to the final product vitellin (Vn). Vn is formed by aggregation of several Vg subunits. In most decapods, the hepatopancreas is the major site of vitellogenin biosynthesis. The production of vitellogenin is controlled by endocrine factors. In this study, the effect of farnesoic acid (FA) and 20-hydroxyecdysone (20E) on production of vitellogenin by hepatopancreas ( HaVg1) was investigated by in vitro organ explant HaVg1 gene expression was stimulated by FA or 20E in a dose-dependent manner. A 2-fold and 2.2-fold increase in HaVg1 gene expression was observed with 4.2 μM FA and 0.7 μM 20E, respectively. The stimulatory effect by either FA or 20E was observed principally during the first 90 min. Stimulation of HaVg1 gene expression by FA and 20E together is greater (3.3-fold increase) than that of either hormone alone. This stimulation was also observed within the first 90 min. To study the synergistic effect of these two hormones, FA and 20E were tested separately and together at low concentration (42.3 nM and 6.7 nM, respectively). Combined use of FA and 20E increased HaVg1 gene expression synergistically, but not additively. These findings should contribute to our understanding of lobster reproduction and provide insights into manipulation of lobster reproduction in aquaculture or under captive conditions.

Suppression of ovarian development and vitellogenin gene expression in the adult diapause of the two-spotted spider mite Tetranychus urticae

Tetranychus urticae (Acari: Tetranychidae) possesses a sac-like ovary with characteristic oocytes that protrude from the ovarian surface. In nondiapause females, transparent oocytes became opaque with yolk deposition between days 0 and 1 in the adult stage at 20 °C. In diapause females, however, ovarian development ceased at a stage having transparent oocytes without yolk deposition; this stage corresponded to the day-0 stage of the nondiapause females. Four partial fragments of the vitellogenin (Vg) genes of T. urticae were isolated. This is the first report on the Vg genes of mites. The deduced amino acid sequences of these four Vg gene fragments contained the von Willebrand factor D domain and the GLCG motif, which were reported to be the common features of Vg sequences in insects and ticks. Northern blot analysis did not detect Vg mRNA in the diapause adult females of T. urticae. It is, therefore, suggested that diapause mites do not synthesize Vg mRNA and that vitellogenesis is regulated at the transcriptional level in diapause.

Time‐Dependent transcriptional profiles of genes of the hypothalamic‐pituitary‐gonadal axis in medaka (Oryzias latipes) exposed to fadrozole and 17β‐trenbolone

Both the anabolic androgen 17beta-trenbolone (TRB) and the aromatase inhibitor fadrozole (FAD) can cause decreased plasma concentrations of estrogen (E2) and reduce fecundity of fish. However, the underlying mechanisms and the molecular pathways involved are largely unknown. The present study was designed to assess time-dependent effects of FAD and TRB on the transcriptional responses of the hypothalamic-pituitary-gonadal (HPG) axis of Japanese medaka (Oryzias latipes). Fourteen-week-old Japanese medaka were exposed to 50 microg FAD/L or 2 microg TRB/L in a 7-d static renewal test, and the expression profiles of 36 HPG axis genes were measured by means of a medaka HPG real-time reverse-transcription polymerase chain reaction array after 8 h, 32 h, or 7 d of exposure. Exposure to TRB or FAD caused lesser fecundity of Japanese medaka and down-regulated transcription of vitellogenin and choriogenin (CHG) gene expression in the liver of females. Exposure to FAD for 8 h resulted in an 8-fold and 71-fold down-regulation of expression of estrogen receptor alpha and choriogenin L (CHG L), respectively, in female liver. 17beta-Trenbolone caused similar down-regulation of these genes, but the effects were not observed until 32 h of exposure. These results support the hypothesis that FAD reduces plasma E2 more quickly by inhibiting aromatase enzyme activity than does TRB, which inhibits the production of the E2 precursor testosterone. Exposure to FAD and TRB resulted in rapid (after 8 h) down-regulation of luteinizing hormone receptor and low-density-lipoprotein receptor in the testis to compensate for excessive androgen levels. Overall, the molecular responses observed in the present study differentiate the mechanisms of the reduced fecundity by TRB and FAD.

Chronic effects of water-borne PFOS exposure on growth, survival and hepatotoxicity in zebrafish: A partial life-cycle test

Perfluorooctane sulfonate (PFOS) is widely distributed and persistent in the environment and wildlife. The main aim of this study was to investigate the impact of long-term exposure to low concentrations of PFOS in zebrafish. Zebrafish fry (F 0, 14 d post-fertilization, dpf) were exposed via the water for 70 d to 0 (control), 10, 50 and 250 μg L −1 PFOS, followed by a further 30 d to assess recovery in clean water. The effects on survival and growth parameters and liver histopathology were assessed. Although growth suppression (weight and length) was observed in fish treated with high concentrations PFOS during the exposure period, no mortality was observed throughout the 70 d experiment. Embryos and larvae (F 1) derived from maternal exposure suffered malformation and mortality. Exposure to 50 and 250 μg L −1 PFOS could inhibit the growth of the gonads (GSI) in the female zebrafish. Histopathological alterations, primary with lipid droplets accumulation, were most prominently seen in the liver of males and the changes were not reversible, even after the fish were allowed to recover for 30 d in clean water. The triiodothyronine (T 3) levels were not significantly changed in any of the exposure groups. Hepatic vitellogenin (VTG) gene expression was significantly up-regulated in both male and female zebrafish, but the sex ratio was not altered. The overall results suggested that lower concentrations of PFOS in maternal exposure could result in offspring deformation and mortality.

Molecular characterization and expression of a novel vitellogenin gene in the freshwater giant prawn, Macrobrachium rosenbergii

Molecular characterization and expression of a novel vitellogenin gene in the freshwater giant prawn, Macrobrachium rosenbergii

Sensory Response System of Social Behavior Tied to Female Reproductive Traits

BackgroundHoney bees display a complex set of anatomical, physiological, and behavioral traits that correlate with the colony storage of surplus pollen (pollen hoarding). We hypothesize that the association of these traits is a result of pleiotropy in a gene signaling network that was co-opted by natural selection to function in worker division of labor and foraging specialization. By acting on the gene network, selection can change a suite of traits, including stimulus/response relationships that affect individual foraging behavior and alter the colony level trait of pollen hoarding. The ‘pollen-hoarding syndrome’ of honey bees is the best documented syndrome of insect social organization. It can be exemplified as a link between reproductive anatomy (ovary size), physiology (yolk protein level), and foraging behavior in honey bee strains selected for pollen hoarding, a colony level trait. The syndrome gave rise to the forager-Reproductive Ground Plan Hypothesis (RGPH), which proposes that the regulatory control of foraging onset and foraging preference toward nectar or pollen was derived from a reproductive signaling network. This view was recently challenged. To resolve the controversy, we tested the associations between reproductive anatomy, physiology, and stimulus/response relationships of behavior in wild-type honey bees.Methodology/Principal FindingsCentral to the stimulus/response relationships of honey bee foraging behavior and pollen hoarding is the behavioral trait of sensory sensitivity to sucrose (an important sugar in nectar). To test the linkage of reproductive traits and sensory response systems of social behavior, we measured sucrose responsiveness with the proboscis extension response (PER) assay and quantified ovary size and vitellogenin (yolk precursor) gene expression in 6–7-day-old bees by counting ovarioles (ovary filaments) and by using semiquantitative real time RT-PCR. We show that bees with larger ovaries (more ovarioles) are characterized by higher levels of vitellogenin mRNA expression and are more responsive to sucrose solutions, a trait that is central to division of labor and foraging specialization.Conclusions/SignificanceOur results establish that in wild-type honey bees, ovary size and vitellogenin mRNA level covary with the sucrose sensory response system, an important component of foraging behavior. This finding validates links between reproductive physiology and behavioral-trait associations of the pollen-hoarding syndrome of honey bees, and supports the forager-RGPH. Our data address a current evolutionary debate, and represent the first direct demonstration of the links between reproductive anatomy, physiology, and behavioral response systems that are central to the control of complex social behavior in insects.