Event Abstract Back to Event Amplification of T cell autoreactivity by OX40/OX40L functional complex on peripheral CD4+ T cells in Graves’ disease Qin Wang1*, Kun X. Zhang1, Yu Ma1 and Guang X. Zhang1 1 Soochow University, Immunology, China As we know, prolonged T cell activation is crucial in the development of Graves’ disease (GD) since the prodution of autoantibody (TRAb) is T-cell dependent. It was reported that some costimulatory receptors and their ligands such as CD28 and CD80 coexpressed on activated T cells and T cells from some autoimmune diseases, enhanced T cell autocrine stimulation. Because OX40L can be induced on activated normal T cells, to address whether the molecule is also expressed on T cells from GD patients, 124 patients with GD, 30 patients with thyroid adenoma and 40 healthy volunteers were recruited. It was demonstrated that OX40 and OX40L were coexpressed on CD4+ T cells, not on CD8+ T cells in this autoimmune disease. OX40, OX40L expression and coexpression levels of them on CD4+ T cells were all notably higher in TRAbh group than that in TRAbn group. Moreover, their expression levels decreased or maintained high with TRAb levels declining or keeping high after therapy. In addition, a higher percentage and longer-term of OX40 and OX40L coexpression was found on activated CD4+ T cells of GD patients than the control group and they showed stronger function to enhance CD4+ T cell proliferation in GD patients. Functional OX40L might interact with functional OX40 molecule expressed on the same T-cell or adjacent T cells, leading to a self-enhancing loop of T cell activation independent of APCs. The data of confocal microscopy and coimmunoprecipitation further showed that OX40 and OX40L formed functional complex which may facilitate signal transduction from OX40L to OX40 and contribute to the development of GD. The results indicated targeting OX40/OX40L signal could be a new therapeutic approach in patients with GD. Keywords: OX40, OX40L, coexpression, Graves Disease, T cells Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Wang Q, Zhang KX, Ma Y and Zhang GX (2013). Amplification of T cell autoreactivity by OX40/OX40L functional complex on peripheral CD4+ T cells in Graves’ disease. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01092 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Qin Wang, Soochow University, Immunology, Suzhou, China, qinwang96@sohu.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Qin Wang Kun X Zhang Yu Ma Guang X Zhang Google Qin Wang Kun X Zhang Yu Ma Guang X Zhang Google Scholar Qin Wang Kun X Zhang Yu Ma Guang X Zhang PubMed Qin Wang Kun X Zhang Yu Ma Guang X Zhang Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.