Expression Of Adenylate Kinase Research Articles

Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen

The present study was conducted for molecular evaluation of bull semen and to study its relationship with different semen characteristics. Fresh semen samples were collected from six breeding bulls. A total of six ejaculates were collected from each of six healthy breeding Jersey bulls at 4 days interval. Immediately after collection, the samples were subjected to physio-morphological and biomolecular evaluation. Percentage of Hypo-osmotic Swelling Test (HOST) positive spermatozoa and acrosome-intact sperms increased with an increase in initial sperm motility. Expression of Chaperonin Containing T-Complex protein 1, subunit 8 (CCT8) gene was found to be negatively correlated with sperm motility, whereas the expression of Adenylate Kinase 1 (AK1) gene did not show any significant relationship with sperm motility.

Expression of adenylate kinase 4 in prostate cancer and its clinical significance

Objective To investigate the expression of adenylate kinase 4 (AK4) in prostate cancer and its clinical significance. Methods The clinical data of 85 patients with prostate cancer who have underwent radical surgery were retrospectively analyzed. The expression of AK4 in prostate cancer tissues and corresponding paracancerous tissues was detected by immunohistochemistry to analyze the relationship with clinicopathological features. Results The positive rates of AK4 in prostate cancer and paracancerous tissues were 85.9% and 38.8% respectively, and the difference was statistically significant (P<0.05). The abnormal expression of AK4 was correlated with recurrence and distant metastasis (all P<0.05). The survival time of AK4-overexpressing patients with prostate cancer was significantly lower than that of AK4 low-expression patients, and the difference was statistically significant (P<0.05). Conclusions The expression of AK4 in prostate cancer tissues is up-regulated, which is related to the distant metastasis of carcinoma and vascular tumor thrombus, which suggests that the expression of AK4 may indicate the poor prognosis of patients with prostate cancer. Key words: Prostate cancer; Immunohistochemistry; Adenylate kinase 4; Prognosis

Expression of adenylate kinase fused MEK1R4F in Escherichia coli and its application in ERK phosphorylation.

To construct a highly expressed and active MEK1R4F (a constitutively active form of mitogen-activated protein kinase kinase 1) by fusion of soluble adenylate kinase (Adk) tag, resulting in Adk-MEK1R4F protein suitable for preparation of phosphorylated ERK. We fused the Adk to the N-terminus of MEK1R4F through overlapping PCR. The expression of Adk-MEK1R4F fusion protein increased ~10-fold in Escherichia coli, and was purified to 95% via two purification steps including Ni-NTA and Q Sepharose fast flow (QFF) chromatography. The purified Adk-MEK1R4F protein was functional for ERK phosphorylation and could use ADP in addition to ATP. The Adk-MEK1R4F had higher catalytic activity than regular MEK1R4F both in vitro and in cell-free extracts system. Adenylate kinase was used as a soluble tag to facilitate MEK1R4F protein expression and its application in large-scale phosphorylated ERK1/2 preparation and purification.

Expression of Adenylate Kinase 1 in Bovine Retinal Cytosol

Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.