Exposition Period Research Articles

Validation of cheap axial passive sampler and procedure suitable for atmospheric NO2 community-based monitoring

Nitrogen dioxide (NO2) is an air pollutant highly impacting on human health, and its measurement is crucial for air quality assessment. Use of passive samplers for long-term large-scale monitoring is a reasonably reliable and economic alternative to more sophisticated and expensive equipment employed in active air sampling by environmental control authorities. In recent years the Citizen Science approach, based on low-cost devices, is spreading more and more in environmental control. Passive samplers available on the market (like the consolidated “Palmes” tubes) are often used in community-based monitoring campaigns. We describe validation of a new cheap axial diffusion tube for NO2 monitoring, used in combination with a new user-friendly App for smartphone that represents an innovation to speed up recording of geo-localization and exposition period data. Affordability and availability of materials, simple construction protocol and easy App procedure, allow possible self-production by school students and non-expert users, making the proposed tube a potential tool to realize extended Citizen Science monitoring campaigns. Accuracy within 25% and precision within 20%, evidenced in validation, show comparability of the tube performance with Palmes-type tubes and agreement with the official monitoring station results. Two small-scale trial monitoring campaigns, involving high school students, were performed to test the efficacy of the proposed “tube-App” system in combining educational impact and community value of air quality monitoring.

Comet Assay and Micronucleus Test in Circulating Erythrocytes of Ctenopharyngodon idella Exposed to Nickel Oxide Nanoparticles.

The number of pollutants released into freshwater and marine environments has increased due to the widespread use of nanoparticles. Nickel oxide nanoparticles (NiO-NPs) were tested for genotoxicity in fish fingerlings of the species Ctenopharyngodon idella. For 7, 14, and 21days, fingerlings were exposed to NiO-NPs with each increasing concentrations of 2.25mg/L, 4.50mg/L, and 6.75mg/L, respectively. The micronuclei assay and comet assay were used to evaluate the DNA damage. The experiment revealed that with the increase in nanoparticle concentration and exposure duration, the level of DNA damage also increased. The experiment resulted to be time and dose dependent, and the damage was found as follows: 6.75mg/L > 4.50mg/L > 2.25mg/L against each exposure period. In terms of comet assay, the results showed that after 7days, the level of DNA damage in all the concentrations was highly significant (P < 0.001). Increased DNA damage was calculated at the higher administered dose of 6.75mg/L for 21days of exposition, followed by 14 and 7days, respectively. The second high toxic effect was observed in the fish blood at the exposure concentration of 4.50mg/L for 21days, followed by 14 and 7days, respectively. The micronuclei induction in the nanoparticle's administered blood could be detected only for a 7-day exposition period. Whereas for the exposed duration of 14 and 21days, the entire red blood cells of the grass carp were completely destroyed demonstrating the ability of the nanoparticles to cause anomalies in aquatic life.

Physiological processes in Phalaenopsis pulcherrima cultivated in hermetically sealed vessels

The hermetic condition is the least studied factor associated with the spaceflights. Phalaenopsis pulcherrima is promising for space farming as it can be cultivated in small substrate blocks, and its photosynthetic apparatus is well adapted to elevated CO2 concentrations and temperatures.Three-year-old meristematic P. pulcherrima plants were planted into plastic (acrylic) vessels filled with fibrous substrate. In control, vessels had an open top. The hermetic conditions were reached by sealing the vessels’ covers with a parafilm. Both control and hermetic vessels were placed in a plant growth chamber where test plants were cultivated under controlled conditions of air temperature, illumination, air humidity, and soil moisture. After 6 and 24 months of cultivation, the CO2 concentration in the hermetic and control vessels was measured, and the physiological characteristics of each test plant, such as the content of macro- and micronutrients, photosynthetic pigments, free amino acids, and content of labile carbohydrates (%) in the leaves of the test-plants were determined.It was revealed that cultivation of P. pulcherrima in hermetic conditions affected its basic physiological processes such as photosynthesis, mineral nutrition, carbohydrates, and amino acid metabolisms. The effect size of this stress factor depended on the duration of exposition period. Long-term cultivation of P. pulcherrima under hermetic conditions promoted the accumulation of nonenzymatic antioxidants (viz. chlorophyll b, carotenoids, and amino acids), which contributed to the adaptation of this orchid species to oxidative stress caused by hermetic environment.

Genotoxicity of Copper, Silver and Green Synthetic Gold Nanoparticles in Fish (Ctenopharyngodon idella).

Grass carp (Ctenopharyngodon idella) was exposed to nanoparticles of different concentrations, i.e., copper oxide nanoparticles (CuO-NPs), silver nanoparticles (Ag-NPs), and green synthetic gold nanoparticles (Au-NPs).The administered doses of the three concentration groups were 20mg L-1, 30 mg L-1, and 40mg L-1 each for a period of 14 and 28 days, respectively. The DNA damage in the erythrocytes of grass carp was detected through a comet assay technique. The values of total comet score (TCS) were noted for the exposed concentrations with a significant increasing trend (p < 0.05) and ordered as CuO-NPs > Ag-NPs > Au-NPs. The highest TCS value of the exposed erythrocytes was recorded for CuO-NPs at 40 mg L-1 after 14 days of exposition period. Comparatively, TCS values of erythrocytes exposed to green synthetic Au-NPs at all the concentrations and exposed time were less as compared to the Ag-NPs and CuO-NPs. The study confirmed the adverse effects of nanoparticles on the genetic material of fish cells.

The Role of the Leishmania infantum Infected Dogs as a Potential Reservoir Host for Toscana Virus in a Zoonotic Visceral Leishmaniasis Focus of Northern Tunisia.

The role of dogs as reservoir hosts for Toscana virus (TOSV) remains undetermined. This study investigated TOSV and Leishmania infantum infections in one healthy and three infected dogs with Leishmania (A, B, C) following natural exposition to sandfly bites in a focus of zoonotic visceral leishmaniasis (ZVL) located in Northern Tunisia from June to October 2020. At the end of the exposition period, infected and healthy dogs were examined for TOSV and L. infantum infections by xenodiagnosis using a colony of Phlebotomus perniciosus. Pools of freshly engorged P. perniciosus at days 0 and those at days 7 post-feeding were screened for TOSV and L. infantum by nested PCR in the polymerase gene and kinetoplast minicircle DNA, respectively. In the exposure site, P. pernicious is the most abundant sandfly species. The infection rates of sandflies with TOSV and L. infantum were 0.10 and 0.05%, respectively. Leishmania infantum DNA and TOSV RNA were detected in P. perniciosus females fed on dog B and C, respectively. The isolation of TOSV in Vero cells was achieved from two pools containing P. perniciosus fed on dog C. No pathogens were detected in P. perniciosus females fed on dog A and on control dog. We report for the first time the reservoir competence of dog with ZVL in the transmission of TOSV to sandfly vectors in natural settings, in addition to its role as a main reservoir host of L. infantum.

Study of the in vitro degradation and characterization of the HaCat keratinocytes adherence on electrospun scaffolds based polyvinyl alcohol/sodium alginate

AbstractBiomaterial scaffolds house and direct cells to grow, exposing them to appropriate growth factors. Most of them are designed to degrade at a controlled rate as the new host tissue replaces them. Here, we show that scaffolds obtained through electrospinning of PVA with 3.5, 4.0, and 5.0 wt% of SA showed a gradual degradation rate under physiological conditions during 100 days of incubation. This behavior is proportional to the SA concentration used, with a mass loss of (41%, 47%, and 49% for each system) after 60 days. Moreover, the dielectric properties (EIS) of the scaffolds correlate with their degradation rate throughout the exposition period (100 days), as indicated by the observed decrease in resistance over time. The 4.0 wt% system showed the lowest capacitance value (1.5 10–5 F) and the highest resistance (4.6106 ohm‐cm2) behavior among all concentrations. Additionally, the morphological analysis through SEM and AFM showed a shrank in the surface morphology after the degradation process and determined the degree of roughness (Ra). Higher roughness prior to the cytocompatibility test promoted increased proliferation and adhesion of HaCat cells, as observed in the 4.0 wt% PVA/SA system (1422 nm Ra). These biomaterials have great potential in wound dressing applications.

Effects of tobacco smoke on indoor air quality: the use of mosses in biomonitoring.

This research was carried out to assess the possibility of using Pleurozium schreberi mosses as bioindicators of atmospheric aerosol pollution in living quarters (kitchen and bedroom), with metals originating from tobacco smoke from various types of cigarettes: conventional cigarettes, e-cigarettes and heated tobacco products. The moss-bag method of active biomonitoring was used. The mosses were exposed in these indoor spaces for three months and, after the exposition period, their analytes - Ni, Cu, Zn, Cd and Pb - were determined using flame atomic absorption spectrometry (F-AAS). Results were interpreted using the relative accumulation factors (RAF), coefficients of variation (CV) and the Wilcoxon test. As a result of the research, it was found that there were statistically significant differences in Zn and Cd concentrations in tobacco smoke from different types of cigarettes. The analyses showed that heated tobacco products contaminate indoor air with metals, similar to conventional cigarettes and e-cigarettes. It was demonstrated that the reliability of biomonitoring results was affected, for example, by the method of preparation of bioindicator samples, such as mosses.

The effects of dietary exposure to Magnéli phase titanium suboxide and titanium dioxide on rainbow trout (Oncorhynchus mykiss)

Magnéli phase titanium suboxides (Magnéli TiOx) are promising, novel materials with superior properties compared to TiO2, they are substoichiometric titanium oxides with the chemical formula TinO2n-1 (where n ≥ 1). In this study, for the first time, subchronic effects of dietary intake of Magnéli TiOx were evaluated and compared with TiO2 particles of similar size, in concentrations 0.1% and 0.01% of feed. The experiment consisted of 38 d of an exposition period and 14 d of a depuration period. Minor effects on plasma biochemical profile and morphological parameters were recorded. A reduced count of leukocytes was found in the blood of both Magnéli TiOx and TiO2 exposed fish, suggesting immunotoxic effects. Erythrocytosis was specific for Magnéli TiOx. Indices of oxidative stress, namely increased lipid peroxidation in liver, increased activity of superoxide dismutase in liver, kidney and gills and glutathione S-transferase (GST) in gills, as well as decreased activity of ceruloplasmin and GST in liver were found predominantly in fish exposed to TiO2. Histopathological examination revealed increased lipid-like vacuolation in the liver, the presence of hyaline droplets in renal tubules and multiplication of mucous glands in the epidermis in both tested substances and intestine damage in TiO2 groups.Overall, in Magnéli TiOx exposed groups, fewer adverse effects compared to TiO2 expositions were recorded. Their wider practical implementation in place of TiO2 is therefore beneficial.

Integrated Monitoring with Moss-Bag and Mussel Transplants in Reservoirs

For the first time, transplants with moss-bags and mussels together were applied to study the water quality in standing water bodies. The tested species: Fontinalis antipyretica Hedw. and Sinanodonta woodiana (Lea, 1834) were collected from unpolluted sites and analyzed to obtain background levels. Then, the moss and mussels were left in cages for a period of 30 days in three reservoirs where both are not present naturally. Two of the reservoirs suffer from old industrial contamination and one is affected by untreated wastes. Twenty-four compounds were studied, among them trace elements Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Zn and organic priority substances: six polybrominated diphenyl ethers (PBDEs) congeners and short-chain chlorinated paraffins (SCCPs). The trace element accumulation was significant after the exposition period in all studied stations. PBDEs and SCCPs were also accumulated up to two times more in the moss tissues. PBDEs in the mussels exceeded the environmental quality standard (EQS). The applied combined transplants, and especially the moss-bags, revealed severe contamination with heavy metals not detected by the water samples. The moss and the mussel followed a different model of trace element and PBDEs accumulation. The SCCPs levels were alarmingly high in all plant samples. The study confirmed PBDEs and SCCPs as bioaccumulative compounds and suggested that an EQS for SCCPs in biota needs to be established.

Cisplatin Changes Expression of SEMA3B in Endometrial Cancer.

Semaphorin 3B (SEMA3B) is characterized as a strong suppressing factor of the proliferation of cancerous cells and also by its anti-angiogenic effect. However, the knowledge on the changes in the expression profile of SEMA3B under the influence of cisplatin in endometrial cancer remains fragmented. The aim of this work was to note the changes in expression of SEMA3B when under the influence of cisplatin in the endometrial cancer cell line. Ishikawa cell line cells were exposed to three different concentrations of cisplatin: 2.5μM; 5μM; 10μM for 12, 24 and 48 hours and were compared to cells untreated by the drug. Changes in the expression profile of SEMA3B were determined based upon RtqPCR (mRNA) alongside the ELISA assay (protein). The Statistica 13.0 PL program was used for statistical analysis (p<0.05). Changes on the transcriptome level seem to be more dynamic than on the proteome level. Regardless of the concentration given or the exposition period, the expression of semaphorin 3B was, in fact, higher in cells exposed to cisplatin. Statistically substantial differences (p<0.05) in the expression of SEMA3B mRNA and protein were seen for all incubation periods at the given cisplatin level when compared to the control. Cisplatin causes a growth in the expression of SEMA3B in an endometrial cancer cell culture, this results in the restoration in the state of cell homeostasis and shows the effectiveness of pharmacotherapy, including a low risk of drug resistance.

A Q fever outbreak associated to courier transport of pets.

On August 3rd, 2017, a Q fever outbreak alert was issued at a courier company that in addition to urgent freight transport offered pet delivery services. The epidemiological investigation set the exposition period between June 1 and August 8. In this period, 180 workers from two operational platforms for parcel distribution located in two provinces of the Basque Country (Bizkaia and Araba) were exposed; 64 filled a questionnaire and provided blood samples for serological testing, resulting in 10 confirmed cases (15.6%) and six (9.4%) probable cases. Nine workers (8 confirmed and 1 probable) showed Q fever symptoms, including pneumonia (five cases), and required medical care services, including one hospital admission. The attack rate was 25% (16/64), being higher among workers that visited the Bizkaia platform. This suggested that the origin of the outbreak was in the Bizkaia platform, where animals in transit waited at a pet holding site until being moved to their destination. Environmental samples consisting on 19 surface dust and two aerosol samples were collected at the Bizkaia platform to investigate the presence of C. burnetti DNA. All dust samples were positive by real time PCR, the lowest Ct values being found in dust collected at the pet holding facilities, and therefore suggesting that contamination originated at the pet holding site. The genotype identified in dust was SNP1/MST13, one of the most commonly identified genotypes in goats and sheep in the Basque Country. During the exposure period, two deliveries of miniature goats were made, of which only one could be investigated and tested negative. Although the contamination source could not be unequivocally identified, transport of ruminants was banned at the company, and Q fever was included among the occupational-associated health risks.

Ácidos hidroxicinámicos en producción animal: farmacocinética, farmacodinamia y sus efectos como promotor de crecimiento. Revisión

El uso de aditivos de origen natural en producción animal ha tomado gran importancia en el sector pecuario, debido a su potencial de promover el crecimiento de una forma similar a los compuestos sintéticos como hormonas y antibióticos, pero sin causar posibles daños a la salud del animal, del consumidor o detrimento en la calidad de la carne. En los aditivos de origen natural existe una amplia variedad de compuestos, que son extraídos de distintas partes de las plantas, donde se toman ciertos aceites esenciales, mezclas de compuestos o compuestos aislados para utilizarse como remedios medicinales o suplementos alimenticios. Dentro de estos extractos, se encuentran los ácidos hidroxicinámicos, presentes en una gran variedad de vegetales, frutas y granos; los cuales presentan interesantes propiedades bioactivas como son, antioxidantes, antimicrobianos, preventivos de enfermedades cardiovasculares e inmunomoduladores. El uso de este tipo de aditivos en producción animal aún es limitado, pero se sugiere que su inclusión puede ser favorable como una estrategia para promover el crecimiento; sin embargo, dos aspectos importantes a estudiarse en los ácidos hidroxicinámicos es su farmacocinética y farmacodinamia, y a partir de allí establecer las condiciones de dosis, períodos de uso y efectos, además las posibles rutas y biotransformaciones que pueden ocurrir en el organismo animal. Esta revisión discute sobre la inclusión de ácidos hidroxicinámicos en dietas de animales de engorda, propiedades farmacocinéticas y farmacodinamias, y los hallazgos como promotores de crecimiento y sus efectos en la calidad de la carne.

Major events and urban development: exploring the spatial impact of China's expositions in the early twentieth century

ABSTRACT Urban developments steered by major events have a long history. Already from the second half of the nineteenth century, World Expositions were mobilized as opportunities for urban upgrading. This article highlights the spatial effects of three Chinese major expositions on their host cities in the early twentieth century (1906–1929). It will in particular highlight the impact on urban development and planning, such as the construction of modern public complexes, the promotion of new urban districts, and the catalysis of structural urban transitions. Considering the significant historical, political, and social analogies, we argue in this article that expositions were adopted under the influence of foreign examples as a model of planning interventions to prompt the modernization of the host cities in China. However, while there was an important transfer of spatial concepts and models, we contend that Chinese authorities played a leading role in importing and exploiting these expositions as strategic instruments. They did so by actively and consciously mobilizing multiple urban actors such as social elites, but also civil society leaders and merchants. This article based on archival research on three expositions, provides novel insights into the urban history of the host cities during the exposition period.

From single GaAs detector to sensor for radiation imaging camera

Our research in the field of bulk SI (semi-insulating) GaAs detectors for digital imaging has taken almost 20 years. We have optimized the process of detector fabrication and improved the detector quality in terms of its detection properties. We have obtained the energy resolution of 7.2% for 122 keV photons decreased to 5.3% by cooling the detector to 255 K. The typical charge collection efficiency of our SI GaAs detectors is between 85 and 88%. Besides, we were developing various X-ray imaging systems. We started with moving one single detector, where we obtained a spatial resolution of 500 µm but the exposition period was of several hours. Later we prepared a digital X-ray scanner composed of 480 strip detectors in one line scanning an area of 12 × 15 cm2 and obtaining the spatial resolution of 250 µm. This fully homemade scanner shortened the exposition time to several minutes. Finally we have fabricated a two-dimensional system of 256 × 256 pixel SI GaAs detectors covering an area of 1.4 × 1.4 cm2 suitable for Timepix read-out chip developed by the MEDIPIX2 collaboration reaching a basic spatial resolution of 55 µm and exposition in the range of not more than a few seconds.

Bioerosion structures in high-salinity marine environments: Evidence from the Al–Khafji coastline, Saudi Arabia

Salinity is one the major stress factors that controls the biotic activities in marine environments. In general, the mixture with fresh-water has been mention as a great stress factor, but the opposite, i.e. high-salinity conditions, is less developed in the ichnological literature. Along the Al-Khafji coastline, Saudi Arabia, hard substrates (constituted by gastropods, bivalves and coral skeletons) contain diverse and abundant bioerosion traces and associated encrusters. Field and laboratory observations allowed the recognition of eight ichnospecies belong to the ichnogenera Gastrochaenolites, Entobia, Oichnus, Caulostrepsis and Trypanites, which can be attributed to various activities produced by bivalves, sponges, gastropods and annelids. The borings demonstrate two notable ichnological boring assemblages, namely, Entobia-dominated and Gastrochaenolites-dominated assemblages. The highly diversified bioerosion and encrustation in the studied hard organic substrate indicate a long exposition period of organic substrate with slow to moderate rate of deposition in a restricted (high-salinity) marine environment. This bioerosion study shows that high-salinity, at least for the study area, is not an important controlling factor for ichnology.

Influence of Aging on Oil Rejuvenated Binder

The presence of aged binder in asphalt mixtures containing high amount of Reclaimed Asphalt Pavement (RAP) is the main reason why the technology of oil rejuvenation (modification) was introduced. When the recycling rate generally exceeds 25 %, it is essential to modify aged binder characteristics in order to guarantee that asphalt mixture performance remains the same as in the case of the virgin mixture. Reclaimed asphalt binder is much stiffer compared to the virgin binder and therefore it is perceived to be susceptible to cracking at low temperatures. The aim of this study is to compare various oil modifiers and evaluate their effects on rheological characteristics before and after the aging. Four different oils were blended together with an artificially aged binder in concentrations which ensure that resulting blends have similar properties as the virgin reference binder. The effect of aging during the production process was simulated by the RTFOT (Rolling Thin Film Oven Test) procedure, long term aging was then simulated by modified RTFOT test with prolonged exposition period. The blends performance assessment was carried out by the frequency sweep test on the Dynamic Shear Rheometer (DSR) and by the evaluation of the critical temperatures on the Bending Beam Rheometer (BBR). The results indicate that addition of oil rejuvenators did not negatively influence binder stiffness at higher temperatures and at the same time enhanced its properties at low temperatures significantly. However, due to the aging effect the difference between stiffness critical temperature and m-value critical temperature increase considerably which might indicate decreasing colloidal stability and lower ability to relax induced stresses.

Efficacy of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) on developmental stages of house fly, Musca domestica.

The housefly, Musca domestica is a major domestic, medical and veterinary pest. The management of these flies reliance on insecticide, causes environmental constraints, insecticide resistance and residues in the meat, skin. Therefore one of the eco-friendly alternate methods is by using biological agents such as entomopathogenic nematodes (EPN). In the present study evaluated the survival of EPN species Steinernema feltiae, Heterorhabditis indica, S. carpocapsae, S. glaseri and S. abbasi in poultry manure and also their efficacy against different developmental stages of house fly. After exposing to poultry manure, S. feltiae showed more survival as followed by H. indica, S. carpocapsae, S. glaseri and S. abbasi in all exposition period. When the exposition period extended to 96h, all nematode species survivability was drastically reduced. After exposing these nematodes to poultry manure at 24h their virulence capacity against wax moth, Galleria mellonella showed all the nematode species were able cause 100% mortality. However their progeny production was significantly reduced. Fly eggs and pupae were refractory to these nematode infection. Petri dish without artificial diet assay showed that, second and 3rd-instar larvae were highly susceptible to EPNs as compared to larvae provided with artificial diet. H. indica showed high virulence capacity compared to other nematodes tested. Poultry manure assay revealed that, H. indica and S. carpocapsae caused minimal mortality where as S. feltiae, S. glaseri and S. abbasi did not cause any mortality. This may be because of poor survival and limited movement of nematodes in poultry manure which may be due to ammonia, other toxic substances in poultry manure. The decrease in larval mortality in manure suggests that biocontrol of housefly by using EPNs is unlikely.

English

Oncidium varicosum is an orchid largely distributed in South America, whose inflorescences with bright yellow flowers are of rare beauty. One of the objectives of the postharvest physiology of flowers is the study of the factors related with the quality loss of cut flowers. The ethylene performs a very important function on the senescence of flowers because it induces abscission of flowers buds and open flowers causing precocious wilting and loss of quality. The sensitivity to ethylene has been reported as variable in function of floral species, exposition period and regulator concentration. The present research evaluated the postharvest of cut inflorescences of O. varicosum ‘Samurai’, after application of ethylene (Ethrel: 1, 10 and 100 mL L-1). The experiment was performed as complete randomized design with factorial scheme composed of two factors: four postharvest treatments and four dates of evaluation. The inflorescences were maintained in the refrigerated ambient. The results demonstrated that the exposition to ethylene caused physiological alterations such as reduction of relative water content of the flowers, decrease of the soluble carbohydrates contents of the petals, increase of the respiration rate and flowers abscission. The concentration of 100 mL L -1 of Ethrel reduced the decorative life of flowers in seven days in relation to the control without product application. while at 1 μL L-1, the flowers presented lower sensitivity to product, with results similar to the control. Key words: Postharvest physiology, orchids, carbohydrates, respiratory rate.

The Appraisal and Analysis of Visitors’ Program Satisfaction in Korea Agriculture Exposition

This study was carried out to analyze the visitors’ program satisfaction, and to propose the direction of improvement in Korea AgricultureExposition. The survey was conducted among 593 visitors during the exposition period from October 18 until October 27. Among visitors, female visitors were 19.4% more than male, the rate of 40s and 50s was high to 53.5%, farmers was most frequent to 26.5%, and residents in Gwangju and Jeonnam were the majority with 84%. The visitors with family as companion form were most frequent to 44.2%, the rate of first visiting was highest to 37.9% in number of visiting, and exposition held information was obtained most frequently to 49.9% by word of mouth. In survey of program contents, visitors preferred the subtropical garden the most among exhibition hall, and 65.9% of visitors were satisfied about guide. The rate of satisfaction was 71.6%, and 32.7% of visitors responded that it did not participate in experience program because there was no guide. In survey of satisfaction in agricultural products purchase, 73.9% of visitors purchased agricultural products, and 86.3% of purchaser were satisfied. The 65.1% of purchaser responded that they bought because of belief about agricultural products, and purchase amount between 10000 and 20000 won was the highest with 33.6%. On the other hand, 41.3% of visitors did not purchase because they do not need. The visitors’ overall satisfaction of in Korea Agriculture Exposition was high to 82.6%, and the most unsatisfactory part appeared to drinking water facilities and parking lot. In survey of development possibility of agriculture through Korea Agriculture Exposition, 86.2% of visitors were positive, and the rate of revisiting intention highly appeared to 80.4%. Keywords: Development possibility, Exhibition hall, Experience program, Program contents, Purchase of agricultural products, Revisiting intention

EFEITO DO NÚMERO E DA IDADE DE OVOS DE Stenoma catenifer NO PARASITISMO POR Trichogramma pretiosum

EFEITO DO NÚMERO E DA IDADE DE OVOS DE Stenoma catenifer NO PARASITISMO POR Trichogramma pretiosum