Background: The improvement of the thermal conductivity of nanofluids is practical for different processes such as drug delivery, manufacturing of crystals, polymer processing, food and drink, cancer treatment, oil and gas, paper making and for many more. The bioconvection phenomenon has engrossed the attention of numerous researchers for its many applications in biotechnology, mechanical and electrical engineering. Bioconvection nanofluids are more prominent in the fields of biomedicine, pharmacy, nanodrug delivery, biomedical, automotive cooling and the military. Purpose: The major purpose of the current work was to determine the numerical and statistical analysis of a novel thermal radiation and exponential space-based heat source on the bioconvective flow of a pseudoplastic 3D nanofluid past a bidirectional stretched Riga surface. The behavior of the Arrhenius activation energy (AAE) and thermal radiation are also disclosed. Methodology: Suitable similarity transformations were used to transmute the partial differential equations of the flow-modeled phenomena into the structure of ordinary differential ones. The numerical solutions for the renewed set of ODEs were tackled by the bvp4c shooting algorithm built-in MATLAB software. Furthermore, the statistical analysis was computed by applying response surface methodology (RSM). Research implications: The numerical analysis is valid for the incompressible three-dimensional, magnetized flow of a pseudoplastic bioconvection nanofluid through a bidirectional surface with Riga plate aspects in the occurrence of activation energy. Social implications: The flow across three dimensions has quite important implementations in various fields, for example, polymer production, material production technology, the manufacturing of nano-biopolymer computer graphics, industry, powered engineering, aeroplane configurations, etc. The current analysis is more applicable in nanotechnology. Results: The consequences of flow control parameters over flow profiles were studied and explained under the graphic structures. Numerical outcomes were computed and discussed in detail. From the results, it was noted that the velocity field was increased via a larger mixed convection parameter. The temperature distribution was boosted via the thermal Biot number. The concentration of nanoparticles declined via the greater Lewis number. Furthermore, the motile microorganisms field was reduced via the Peclet number. Originality: Until now, no investigation has been recognized to examine the consequences of the bioconvection flow of three-dimensional pseudoplastic nanofluids past a Riga plate containing motile microorganisms utilizing the shooting method called bvp4c. Conclusions: From the results, it was concluded that nanofluids are more helpful for heat transfer increments. Furthermore, from the experimental design observed, the response declined via the thermophoresis parameter, which was significant from the ANOVA observed model.