The study presents an analysis of S355J2+N steel and AA5083 aluminum alloy welded structural joints using explosion welded transition joints of reduced thickness. The transition joint thickness reduction significantly hinders the welding of the joints due to the risk of damage to the Al/steel interface as a result of the high temperatures during welding. In the previous article, the strength of the transition joint was analyzed but ship structures, apart from static loads, are subjected to many different cyclical loads. Welded structural joints are analyzed to determine the welding influence on the fatigue life and fracture type of the transition joints. The results of the fatigue tests show that the fatigue damage in the specimens occurs in the aluminum welded joint, and not in the explosively welded joint. The damage obtained was characteristic of cruciform welded joint specimens and both types of root and toe damage occurred. Based on the obtained results, fatigue curves for the joint were determined and compared to the fatigue curves for the AA5083 base material. The experimental fatigue curve was also compared with the design curve for welded aluminum structures from Eurocode. The conducted analysis showed the possibility of using Al/steel explosion welded transition joints of reduced thickness to transfer cyclical loads.