Exploratory Walking Research Articles

Route learning and transport of resources during colony relocation in Australian desert ants.

Many ant species can respond to dramatic changes in local conditions by relocating the entire colony to a new location. While we know that careful learning walks enable the homing behavior of foraging ants to their original nest, we do not know whether additional learning is required to navigate to the new nest location. To answer this question, we investigated the nest relocation behavior of a colony of Australian desert ants (Melophorus bagoti) that relocated their nest in response to heavy rainfall in the semidesert terrain of Alice Springs. We identified five types of behavior: exploration between nests (Old-to-New nest and New-to-Old nest), transport from Old to New nest, and relearning walks at Old and New nests. Initially, the workers performed relearning walks at the Old nest and exploratory walks between the Old and New nests. Once they completed the exploratory walks, the workers transported resources and brood to the new nest. Finally, we observed the workers performing relearning walks at the New nest. While the relearning walks at the Old nest were slow and appear to enable exploratory walks to the New nest, the relearning walks at the new nest were faster and appeared to enable homing from foraging trips. These observations shed insight on how learning helps these ants to respond to sudden changes in their environment.

Freezing of movements and its correspondence with MLG1 neurons response to looming stimuli in the crab Neohelice.

Upon visually detecting a moving predator animals often freeze, i.e. stop moving, to minimize being uncovered and to gather detailed information of the object's movements and properties. In certain conditions the freezing behavior can be enough to avoid a predatory menace but, when the risk is high or increases to a higher level, animals switch strategy and engage in an escape response. The neural bases underlying escape responses to visual stimuli are extensively investigated both in vertebrates and arthropods. However, those involved in freezing behaviors are much less studied. Here, we investigated the freezing behavior displayed by the crab Neohelice granulata when confronted with a variety of looming stimuli simulating objects of distinct sizes approaching on a collision course at different speeds. The experiments were performed in a treadmill-like device. Animals engaged in exploratory walks respond to the looming stimulus with freezing followed by escaping. The analysis of the stimulus optical variables shows that regardless of the looming dynamic, the freezing decision is made when the angular size of the object increases by 1.4°. In vivo intracellular recording responses of Monostratified Lobula Giant Neurons (MLG1) to the same looming stimuli show that the freezing times correlate with the times predicted by a hypothetical spike counter of this neuron.

A city in the process of revitalisation in the opinion of young people from disadvantaged neighbourhoods. Results of a pilot qualitative study

The article presents the results of a qualitative pilot study conducted to reconstruct both the representation of the city from the perspective of young people from disadvantaged neighbourhoods (DN) in Łódź (Poland) and the configuration of its determinants. Exploration of the city, regulated by the principles of the qualitative content analysis method included exploratory walks (research participant + two students) and a discussion with a group of youth from DN – participants of a youth club established in one of the local educational institutions. The study results present a city full of spatial divisions in which the disadvantaged position of young people from DN becomes evident. The reconstructed picture of the relationship between space and places reveals discursive, cultural and economic mechanisms of extending the power of some groups of citizens over others, whose intersectionality in the era of intensification of revitalisation processes is reflected in the identity processes of these young people. The conclusions of the study encourage its continuation on a larger and more purposefully diverse research sample as well as a revitalised discussion around the role of social professions in revitalisation processes.

Regulation of sensory perception and motor abilities by brain-specific action of chromatin remodeling factor CHD1.

The ATP-dependent chromatin remodeling factor CHD1 (chromodomain-helicase-DNA binding protein 1) is involved in both the de novo assembly and the remodeling of chromatin. Recently, we discovered a crucial role of CHD1 in the incorporation of the histone variant H3.3 in the fly brain illustrated by widespread transcriptional upregulation and shortened lifespan in Chd1-mutant animals. Because many genes linked to sensory perception were dysregulated in Chd1-mutant heads, we studied the role of CHD1 in these processes. Here we show that Chd1-mutant flies have severe defects in their response behavior to olfactory and gustatory but not visual stimuli. Further analyses suggested that poor performance in gustatory response assays was caused by reduced motivation for foraging and feeding rather than defects in taste perception. Moreover, we show that shortened lifespan of Chd1-mutant flies is accompanied by indications of premature functional aging as suggested by defects in negative geotaxis and exploratory walking assays. The latter phenotype was rescued by neuronal re-expression of Chd1, while the olfactory defects were not. Interestingly, we found evidence for indirect regulation of the non-neuronal expression of odorant binding proteins (Obp) by neuronal expression of Chd1. Together, these results emphasize the crucial role of CHD1 activity controlling diverse neuronal processes thereby affecting healthy lifespan.

Reduced Insulin Signaling Targeted to Serotonergic Neurons but Not Other Neuronal Subtypes Extends Lifespan in Drosophila melanogaster.

Reduced Insulin/IGF-like signaling (IIS) plays an evolutionarily conserved role in improving longevity and some measures of health-span in model organisms. Recent studies, however, have found a disconnection between lifespan extension and behavioral health-span. We have previously shown that reduction of IIS in Drosophila neurons extends female lifespan but does not improve negative geotaxis senescence and has a detrimental effect on exploratory walking senescence in both sexes. We hypothesize that individual neuronal subtypes respond differently to IIS changes, thus the behavioral outcomes of pan-neuronal IIS reduction are the balance of positive, negative and neutral functional effects. In order to further understand how reduced IIS in neurons independently modulates lifespan and locomotor behavioral senescence we expressed a dominant negative Insulin receptor transgene selectively in individual neuronal subtypes and measured the effects on lifespan and two measures of locomotor senescence, negative geotaxis and exploratory walking. IIS reduction in cholinergic, GABAergic, dopaminergic, glutamatergic, and octopaminergic neurons was found to have either no affect or a detrimental effect on lifespan and locomotor senescence. However, reduction of IIS selectively in serotonergic neurons resulted in extension of lifespan in females with no effect on locomotor senescence. These data indicate that individual neuronal subtypes respond differently to IIS changes in the modulation of lifespan and locomotor senescence, and identify a specific role for the insulin receptor in serotonergic neurons in the modulation of lifespan.

Exploratory walk and local cohesion— the concept and application

ABSTRACT The article outlines the concept of an exploratory walk as a method of research and co-creating social cohesion in the local context. The term exploratory walk refers to the mobile participatory research method, which is connecting cognitive aims with practical during the walks in pairs or groups. This method goes beyond the formula of ‘walking interview’, offering the participants an active role as experts or guides in the space familiar to them. The article discusses the following distinguished potentials of exploratory walks: cognitive, emotional, participatory, collaborative and transformative. They are analysed and assessed as possible to use in the study and co-creation of local cohesion, which is defined as ties between inhabitants. These ties are expressed by the ability to launch and develop positive relations, manage and share resources, and regulate the behaviour of co-citizens. Analyses presented here describe this method as promising from the possibility of knowing and strengthening social cohesion on the local scale. The concept is illustrated with an example of research on revitalisation. Moving together in cognised space – as shown in the empirical illustration – stimulates participants to perceive spatial manifestations of local cohesion or its deficits, to involvement, participation, collaboration and acting for change.

Behavioral patterns in the early-stage antipredator response change after tail autotomy in adult wall lizards.

Autotomy is a drastic antipredator defense consisting of the voluntary shedding of a body part to escape from the predators. The loss of a body part may impair locomotion, feeding or mating, so animals may face a higher predation risk shortly after autotomy. Thus, until regeneration is completed, prey may adjust their behavior to reduce predation risk, and this could involve secondary costs. We assessed the effect of tail loss on the antipredator behavior of wall lizards (Podarcis muralis), comparing the behavior of tailed and tailless individuals exposed to a predatory snake (Coronella austriaca) scent, under controlled experimental conditions. Tailless lizards spent significantly more time performing behaviors with antipredatory significance (e.g., moving slowly), whereas tailed individuals performed exploratory walking for significantly more time. Moreover, tailless lizards spent more time basking, which probably increases the effectiveness of their cryptic design and decreases detection by predators. Lizards intensified the tongue flick rates when exposed to a pungent control or snake scents, as compared to their response to a neutral control. Besides, both tailed and tailless lizards intensified some aspects of their antipredator behavior (walking slowly and avoiding refuge use) when exposed to snake scent, which indicates discrimination of the smell of predatory snakes. Lizards decreased refuge use when exposed to predator scents, probably because the refuges are evaluated as unsafe due to a high concentration of snake scents. To conclude, our experiments showed that, after losing their tails, wall lizards modify their behavior in a way that likely minimizes predation risk.

Insect locomotion: Flies show you how to stay on course

Walking animals are faced with making a trade-off between maintaining a stable posture and gait and pursuing other goals such as keeping a straight path. A new study on exploratory walking in flies provides a sophisticated quantitative account of this behavioural problem, with some intriguing discoveries.

Fast tuning of posture control by visual feedback underlies gaze stabilization in walking Drosophila

SummaryLocomotion requires a balance between mechanical stability and movement flexibility to achieve behavioral goals despite noisy neuromuscular systems, but rarely is it considered how this balance is orchestrated. We combined virtual reality tools with quantitative analysis of behavior to examine how Drosophila uses self-generated visual information (reafferent visual feedback) to control gaze during exploratory walking. We found that flies execute distinct motor programs coordinated across the body to maximize gaze stability. However, the presence of inherent variability in leg placement relative to the body jeopardizes fine control of gaze due to posture-stabilizing adjustments that lead to unintended changes in course direction. Surprisingly, whereas visual feedback is dispensable for head-body coordination, we found that self-generated visual signals tune postural reflexes to rapidly prevent turns rather than to promote compensatory rotations, a long-standing idea for visually guided course control. Together, these findings support a model in which visual feedback orchestrates the interplay between posture and gaze stability in a manner that is both goal dependent and motor-context specific.

How are allotment gardens managed? A comparative study of usage and development in contemporary urban space in Germany and Poland

Abstract This article aims to analyse the development and use of allotment gardens (AGs) and plots in Westphalia and Lippe (Germany) and Wielkopolska (Poland) to assess what functions are feasible in their current stage, thus, contributing to an ongoing discussion on the role of AGs in contemporary urban space. The analysis considered ecosystem services, green infrastructure and urban agriculture. Characteristic features of AGs were identified by means of a survey of plot-holders, in-depth interviews with representatives of allotment associations, and exploratory walks. The management and use of AGs differs in both regions in terms of accessibility, common areas, impact on the landscape and plots development. AGs in Westphalia and Lippe are accessible and developed in a way which meets needs of external users. They fit harmoniously into the surrounding landscape. However, AGs in Wielkopolska are not as accessible, prioritise internal users, and do not always blend in with their surroundings. In Westphalia and Lippe, in addition to recreational and edible plant-growing plots, there are also educational and integration ones, while in Wielkopolska main categories of plots were more numerous and varied; from recreational with a predominance of ornamental plants to fully-cultivated. However, the considerable freedom that Polish plot-holders enjoy gives rise to the gradual marginalisation of edible-plant growing.

Learning walks in an Australian desert ant, Melophorus bagoti.

ABSTRACTThe central Australian ant Melophorus bagoti is the most thermophilic ant in Australia and forages solitarily in the summer months during the hottest period of the day. For successful navigation, desert ants of many species are known to integrate a path and learn landmark cues around the nest. Ants perform a series of exploratory walks around the nest before their first foraging trip, during which they are presumed to learn about their landmark panorama. Here, we studied 15 naive M. bagoti ants transitioning from indoor work to foraging outside the nest. In 3–4 consecutive days, they performed 3–7 exploratory walks before heading off to forage. Naive ants increased the area of exploration around the nest and the duration of trips over successive learning walks. In their first foraging walk, the majority of the ants followed a direction explored on their last learning walk. During learning walks, the ants stopped and performed stereotypical orientation behaviours called pirouettes. They performed complete body rotations with stopping phases as well as small circular walks without stops known as voltes. After just one learning walk, these desert ants could head in the home direction from locations 2 m from the nest, although not from locations 4 m from the nest. These results suggest gradual learning of the visual landmark panorama around the foragers’ nest. Our observations show that M. bagoti exhibit similar characteristics in their learning walks to other desert ants of the genera Ocymyrmex and Cataglyphis.

Mushroom Body Extrinsic Neurons in Walking Bumblebees Correlate With Behavioral States but Not With Spatial Parameters During Exploratory Behavior.

Central place foraging insects like honeybees and bumblebees learn to navigate efficiently between nest and feeding site. Essential components of this behavior can be moved to the laboratory. A major component of navigational learning is the active exploration of the test arena. These conditions have been used here to search for neural correlates of exploratory walking in the central arena (ground), and thigmotactic walking in the periphery (slope). We chose mushroom body extrinsic neurons (MBENs) because of their learning-related plasticity and their multi-modal sensitivities that may code relevant parameters in a brain state-dependent way. Our aim was to test whether MBENs code space-related components or are more involved in state-dependent processes characterizing exploration and thigmotaxis. MBENs did not respond selectively to body directions or locations. Their spiking activity differently correlated with walking speed depending on the animals’ locations: on the ground, reflecting exploration, or on the slope, reflecting thigmotaxis. This effect depended on walking speed in different ways for different animals. We then asked whether these effects depended on spatial parameters or on the two states, exploration and thigmotaxis. Significant epochs of stable changes in spiking did not correlate with restricted locations in the arena, body direction, or walking transitions between ground and slope. We thus conclude that the walking speed dependencies are caused by the two states, exploration and thigmotaxis, rather than by spatial parameters.

The development process and effects from the management of community gardens in two post-socialist cites: Bratislava and Poznań

Community gardens are perceived as one of many forms of urban agriculture and gardening practices using high levels of social innovation and environmentally-friendly lifestyles. In contrast to the years of experience in the US and Western European countries, community gardens are rather new in Central and Eastern European countries. Our research strives to contribute to the lively discussion of the development process and effects from the management of community gardens in the urban environment, especially through a comparative analysis of community gardens in two post-socialist countries, Slovakia and Poland, with an emphasis on Bratislava and Poznań. In order to better understand the nuances of community gardening in these areas, we carried out extensive field research involving exploratory walks, participatory observation and semi-structured interviews with community garden representatives in both cities. The results of our analysis revealed both similarities and differences in the city-specific approaches in the ways community gardens are established, managed and developed under purportedly similar historical, geographical and socio-economic conditions of two post-socialist cities. We conclude that a strictly copy-paste approach does not exist. In other words, the proper way of management of community gardens and even the perception and interpretation of what is considered a community garden can vary from one place to another and is usually negotiated in the place-specific local context.

Floristic survey of ornamental plants used in Dom Delgado University City at the Universidade Federal do Maranhão, São Luís, Maranhão State, Brazil

The objective of this study is to list the flora used in ornamentation, afforestation and landscaping in the common areas of the Dom Delgado Campus (São Luís) at the Universidade Federal do Maranhão (2º32’20.7”S 44º16’58.4”W), where this study was carried out. The collection took place through exploratory walks, including individual specimens planted there and those that were kept during the architectonical development of the University. Identification was obtained using analytical keys, specialized literature and the comparison with material previously identified in the Herbarium of Maranhão (MAR) where the exsiccates produced in the present study were deposited. 70 species, 56 genres and 29 families were found. Fabaceae (with 10 species), Apocynaceae (with nine spp.); Malvaceae and Rubiaceae (six spp.); Arecaceae (four spp.); Araceae, Bignoniaceae and Heliconiaceae with three species each were the most representative families in terms of the number of species. Among the total number of species identified, it was noted that there was no great difference in the quantity of native Brazilian species (38%), when compared to exotic species (34%); the remaining species were classified as cultivated (17%) and naturalized (11%). In conclusion, it is clear that the afforestation/ landscaping of the Dom Delgado Campus, follows a method of randomization in relation to the planting of the species used.

Behavioral Senescence and Aging-Related Changes in Motor Neurons and Brain Neuromodulator Levels Are Ameliorated by Lifespan-Extending Reproductive Dormancy in Drosophila.

The lifespan of Drosophila melanogaster can be extended substantially by inducing reproductive dormancy (also known as diapause) by lowered temperature and short days. This increase of longevity is accompanied by lowered metabolism and increased stress tolerance. We ask here whether behavioral senescence is ameliorated during adult dormancy. To study this we kept flies for seven or more weeks in normal rearing conditions or in diapause conditions and compared to 1-week-old flies in different behavioral assays of sleep, negative geotaxis and exploratory walking. We found that the senescence of geotaxis and locomotor behavior seen under normal rearing conditions was negligible in flies kept in dormancy. The normal senescence of rhythmic activity and sleep patterns during the daytime was also reduced by adult dormancy. Investigating the morphology of specific neuromuscular junctions (NMJs), we found that changes normally seen with aging do not take place in dormant flies. To monitor age-associated changes in neuronal circuits regulating activity rhythms, sleep and walking behavior we applied antisera to tyrosine hydroxylase (TH), serotonin and several neuropeptides to examine changes in expression levels and neuron morphology. In most neuron types the levels of stored neuromodulators decreased during normal aging, but not in diapause treated flies. No signs of neurodegeneration were seen in either condition. Our data suggest that age-related changes in motor neurons could be the cause of part of the behavioral senescence and that this is ameliorated by reproductive diapause. Earlier studies established a link between age-associated decreases in neuromodulator levels and behavioral decline that could be rescued by overexpression of neuromodulator. Thus, it is likely that the retained levels of neuromodulators in dormant flies alleviate behavioral senescence.

An experience-based learning framework

Purpose This paper aims to present an experience-based learning framework that provides a bottom-up, student-centered entrance point for the development of systems thinking, normative and collaborative competencies in sustainability. Design/methodology/approach The framework combines mental mapping with exploratory walking. It interweaves mapping and walking activities with methodological and theoretical inputs as well as with reflections and discussions. The framework aligns experiential activities, i.e. mental mapping and walking, with learning objectives, i.e. novice-level sustainability competencies. The authors applied the framework for student activities in Phoenix/Tempe and Hamburg/Lüneburg as part of The Global Classroom, a project between Arizona State University in the USA and Leuphana University of Lüneburg in Germany. Findings The application of the experience-based learning framework demonstrates how students started developing systems thinking (e.g. understanding urban systems as functional entities and across different domains), normative (e.g. using different sustainability principles) and collaborative (e.g. learning across disciplinary, social and cultural differences) competencies in sustainability. Originality/value The experience-based learning framework contributes to the development of curricular activities for the initial development of sustainability competencies in introductory-level courses. It enables students from different disciplinary, social and cultural backgrounds, e.g. in international education, to collaboratively start developing such competencies. The framework can be adapted to different educational contexts.

Feeding and metabolic compensations in response to different foraging costs

How energetic cost of locomotion affects foraging decisions, and its metabolic consequences are poorly understood. In several groups of animals, including hermit crabs, exploratory walking enhances the efficiency of foraging by increasing the probability of finding more and better food items; however, the net gain of energy will only be enhanced if the costs of walking are lower than the benefits of enhanced food acquisition. In hermit crabs, the cost of walking increases with the mass of the shell type occupied. Thus, we expected that hermit crabs should adjust their foraging strategy to the cost of movement in different shells. We assessed the foraging, the quantity and quality of food intake, and the energetic cost of maintenance of hermit crabs paying different costs of foraging in the wild. The exploratory walking negatively correlated with shell mass, showing that hermit crabs use different foraging strategies in response to the expenditure required to move. Hermit crabs deal with high energetic costs of foraging in heavy shells by reduces their exploratory walking and overall metabolic rate, as a strategy to maximize the net energy intake. This study integrates behavioral and metabolic compensations as a response to foraging at different costs in natural conditions.

Intergenerational and intercultural civic learning through storied child‐led walks of Chiang Mai

AbstractRecent times have witnessed global trends of increased protection of children in public spaces. The participatory arts project The Walking Neighbourhood hosted by Children renegotiates child agency in public spaces by inviting primary school aged children to curate and lead adult audiences on walks of local neighbourhoods. Multiple cities across Australia, Asia, and Europe have hosted The Walking Neighbourhood since 2012. This article focuses on one aspect of that initiative: the Australian‐Thai research collaboration for the Chiang Mai child‐hosted walks. Through storytelling, the Australian and Thai authors share their sensorial ethnographic encounters of two child‐led walks in Chiang Mai to provide lived sensorial affective accounts of children's perceptions and engagement with public spaces. These stories demonstrate how the project provides education for children's independently mobile engagement with their neighbourhoods and public spaces, in that the children competently managed responsibility for their adult audiences, and embraced responsibility for sharing their emplaced connections with a neighbourhood locale. Through participatory arts practice, artists, child hosts, and adult audience members co‐construct and interpret exploratory walks of local neighbourhoods to enable enhanced independent mobilities for children, challenging the norms that assert controlled childhoods. Such interdisciplinary, intergenerational, and intercultural experiences can enable reconceptualisation of children and public spaces and new realities for civic engagement and learning for all. © 2016 Institute of Australian Geographers

The Drosophila insulin receptor independently modulates lifespan and locomotor senescence.

The Insulin/IGF-like signalling (IIS) pathway plays an evolutionarily conserved role in ageing. In model organisms reduced IIS extends lifespan and ameliorates some forms of functional senescence. However, little is known about IIS in nervous system ageing and behavioural senescence. To investigate this role in Drosophila melanogaster, we measured the effect of reduced IIS on senescence of two locomotor behaviours, negative geotaxis and exploratory walking. Two long-lived fly models with systemic IIS reductions (daGAL4/UAS-InRDN (ubiquitous expression of a dominant negative insulin receptor) and d2GAL/UAS-rpr (ablation of insulin-like peptide producing cells)) showed an amelioration of negative geotaxis senescence similar to that previously reported for the long-lived IIS mutant chico. In contrast, exploratory walking in daGAL4/UAS-InRDN and d2GAL/UAS-rpr flies declined with age similarly to controls. To determine the contribution of IIS in the nervous system to these altered senescence patterns and lifespan, the InRDN was targeted to neurons (elavGAL4/UAS-InRDN), which resulted in extension of lifespan in females, normal negative geotaxis senescence in males and females, and detrimental effects on age-specific exploratory walking behaviour in males and females. These data indicate that the Drosophila insulin receptor independently modulates lifespan and age-specific function of different types of locomotor behaviour. The data suggest that ameliorated negative geotaxis senescence of long-lived flies with systemic IIS reductions is due to ageing related effects of reduced IIS outside the nervous system. The lifespan extension and coincident detrimental or neutral effects on locomotor function with a neuron specific reduction (elavGAL4/UAS-InRDN) indicates that reduced IIS is not beneficial to the neural circuitry underlying the behaviours despite increasing lifespan.

Neuronal control of locomotor handedness in Drosophila

Genetically identical individuals display variability in their physiology, morphology, and behaviors, even when reared in essentially identical environments, but there is little mechanistic understanding of the basis of such variation. Here, we investigated whether Drosophila melanogaster displays individual-to-individual variation in locomotor behaviors. We developed a new high-throughout platform capable of measuring the exploratory behavior of hundreds of individual flies simultaneously. With this approach, we find that, during exploratory walking, individual flies exhibit significant bias in their left vs. right locomotor choices, with some flies being strongly left biased or right biased. This idiosyncrasy was present in all genotypes examined, including wild-derived populations and inbred isogenic laboratory strains. The biases of individual flies persist for their lifetime and are nonheritable: i.e., mating two left-biased individuals does not yield left-biased progeny. This locomotor handedness is uncorrelated with other asymmetries, such as the handedness of gut twisting, leg-length asymmetry, and wing-folding preference. Using transgenics and mutants, we find that the magnitude of locomotor handedness is under the control of columnar neurons within the central complex, a brain region implicated in motor planning and execution. When these neurons are silenced, exploratory laterality increases, with more extreme leftiness and rightiness. This observation intriguingly implies that the brain may be able to dynamically regulate behavioral individuality.