Age is expected to influence women’s sexual functioning and behavior, not only due its impact at the organic level, but also given the strong cohort effects that shape women’s beliefs regarding sexuality and relationships. However, most of the current assumptions about the effects of age in women’s sexual response and behavior, relate to the influence of health issues on the propensity for sexual difficulties. Indeed, broader aspects such as sexual and relationship satisfaction, have only recently fallen under the scope of scientific research. Similarly, other aspects relating to the appraisal of sexual contents by older women have been completely dismissed. Accordingly, the aim of the current study was to test the effect of age on the sexual and emotional responses to sex stimuli, as well as to test the adding effects of women’s beliefs regarding sexuality, on the appraisal of these stimuli. Seventy-three heterosexual women, ranging from 18-77 years old, were exposed to a series of romantic, sexually moderate and sexually explicit pictures in a laboratorial context. Women reported on the levels of emotional valence, subjective sexual arousal, and level of sexual content attributed to the pictures in each set. Additionally, women completed a self-report questionnaire aimed at measuring women’s beliefs regarding female sexuality.