Abstract This article explores sumptuary legislation and its enforcement in early modern Genoa. Whereas the sumptuary laws from other early modern Italian city-states partitioned society by social rank, profession, or citizenship, the laws in effect in late sixteenth-century Genoa divided the population only into men and women. Genoa therefore represents a key site for exploring gender difference through dress and adornment. Adopting a gendered, sociological, and material culture framework, this article demonstrates how sumptuary laws informed understandings of gender and gendered practices of dressing. It takes as its point of departure a ledger of sumptuary denouncements for the year 1594, and examines how Genoa’s citizens adhered to, or transgressed the gendered expectations set out by the city’s legislative structures. It argues that while prescriptions for idealized masculine and feminine comportment coloured the content and wording of the law, in daily life a spectrum of gendered identities could be enacted through clothing. This article thus advances discourse on the impact of sumptuary laws on understandings of gender in early modern Italy, and the ways in which masculine and feminine identifications were negotiated through and in dialogue with clothing.