Abstract Hadron calorimeters are one of the most important detectors in the contemporary nuclear and particle physics experiments. Their construction and verification process takes years in designing, testing and data analysis of prototypes. Crucial step for the prototypes are tests performed by exposing the detectors to charge particle beams. Here we study the impact of the different beam content composition to one of the main properties of the hadron calorimeters – their energy resolution. We implement a test detector - Metal Hadronic Calorimeter (MACA) in GEANT4 environment and we perform different simulations to study the influence to the main energy resolution parameters.