Experimental Research Research Articles

LncRNA MEG3: Targeting the Molecular Mechanisms and Pathogenic causes of Metabolic Diseases.

Non-coding RNA is a type of RNA that does not encode proteins, distributed among rRNA, tRNA, snRNA, snoRNA, microRNA and other RNAs with identified functions, where the Long non-coding RNA (lncRNA) displays a nucleotide length over 200. LncRNAs enable multiple biological processes in the human body, including cancer cell invasion and metastasis, apoptosis, cell autophagy, inflammation, etc. Recently, a growing body of studies has demonstrated the association of lncRNAs with obesity and obesity-induced insulin resistance and NAFLD, where MEG3 is related to glucose metabolism, such as insulin resistance. In addition, MEG3 has been demonstrated in the pathological processes of various cancers, such as mediating inflammation, cardiovascular disease, liver disease and other metabolic diseases. To explore the regulatory role of lncRNA MEG3 in metabolic diseases. It provides new ideas for clinical treatment or experimental research. In this paper, in order to obtain enough data, we integrate and analyze the data in the PubMed database. LncRNA MEG3 can regulate many metabolic diseases, such as insulin resistance, NAFLD, inflammation and so on. LncRNA MEG3 has a regulatory role in a variety of metabolic diseases, which are currently difficult to be completely cured, and MEG3 is a potential target for the treatment of these diseases. Here, we review the role of lncRNA MEG3 in mechanisms of action and biological functions in human metabolic diseases.

Penetration test and numerical simulation of scaled earth penetrator for coral aggregate seawater concrete cylindrical target

In order to study the mechanical properties of coral aggregate seawater concrete (CASC) subjected to the penetration of scaled earth penetrator, firstly, the penetration depth and damage of CASC and ordinary sand-gravel concrete under the same conditions were compared by experimental research, and the influence of concrete strength and projectile velocity on penetration performance was analyzed. Then, the three-dimensional mesoscopic model is used to simulate the test conditions, and the failure process and failure mechanism are analyzed. The results show that under the same penetration conditions, as the strength of CASC increases, the crater diameter becomes larger, which is consistent with the conclusion of ordinary concrete (OPC). During the penetration process, the CASC and the mortar are destroyed as a whole. At the same time, as the penetration speed of the projectile increases, the width of the crack on the surface of the target increases. When the projectile velocity is low, the anti-penetration performance of CASC with the same strength is not as good as that of OPC with the same strength. For the target with a thickness of 25 cm, the penetration depth of the concrete target with the same strength will increase with the increase of the velocity of the projectile. When the velocity of the projectile is constant, the penetration depth of CASC will decrease with the increase of the strength. The numerical simulation results obtained by using the K & C mesoscopic model are in good agreement with the experimental results, indicating that the model and its model parameters have high reliability in the analysis of CASC penetration mechanical properties.

Experimental research on the degradation law of the bond performance between steel bars and concrete with rust expansion cracking

Experimental research on the degradation law of the bond performance between steel bars and concrete with rust expansion cracking

An integrative literature review: design and stages of peace group counselling as peace counselor/educator strategies for fostering a peace mindset

Counsellors as peace educators are to foster a peaceful mindset to students. However, not every peace counsellor shares peaceful thinking with clients or students with problems as a gap. Peace group leaders can integrate counselling as a science with religion through the design and stages of peace group counselling (PGC). This research aims to design and organize PGC stages to facilitate peaceful thinking patterns in peace counsellors/educators (PC/E) to foster peaceful thinking patterns. Integrative literature review as a research model by collecting data through documentation with relevant books and journals, then analyzing it descriptively and qualitatively. The research findings found that the PC/E's duties as imams (group leaders) and kaffah individuals have therapeutic abilities. PC/E combines peaceful thinking with help from God. The PGC process has three stages: initial, work, and the final. The work stages combine the sub-stages of peace counselling, starting from rational, exploring the root causes of peaceful thoughts, self-reflection, seeking, and searching for peaceful thought patterns, looking for different forms and behaviors of peaceful thought patterns towards God's teachings as a novelty, and ending with evaluation and follow-up. PC/E implements the PGC design and stages so that individuals can cultivate a peaceful mindset through training and experimental research.

Stabilizing Pipe Flow by Flattening the Velocity Profile

It is important to control turbulence in industrial processes. Past experimental and numerical researches have shown that a turbulent puff in pipe flow can be removed or delayed by flattening the profile of the upstream velocity because a flattened velocity profile causes the point of inflection on it to collapse. The energy gradient theory has been developed to study turbulent transition, and the relevant studies have shown that turbulence arises due to the generation of singularities in the flow field. In pressure-driven flows like the pipe flow, the point of inflection on the velocity profile leads to the appearance of a singular point in the unsteady Navier–Stokes equation. In this study, the energy gradient theory is used to demonstrate why the point of inflection on the profile of velocity of pipe flow is the critical point for generating turbulence. Then, it is shown how flattening the velocity profile leads to the elimination of the point of inflection on the velocity profile of pipe flows to delay turbulent transition. It is also clarified why this technique is not effective at higher Reynolds number because the flattened velocity profile violates the criterion for flow stability relating to transition to turbulence.

Derajat Aglutinasi pada Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Masa Simpan Test Sel A dan Test Sel B Hari Ke-0, Ke-2, Ke-4, Ke-6 dan Ke-8

Blood group examination is an examination that aims to determine the type of blood group. Cell test is a blood group examination reagent used to detect antibodies in the serum being examined. The long shelf life of cell tests that can only last for two days is considered less effective for agencies with a high level of blood services. The purpose of this study was to describe the degree of agglutination in blood group examination with cell test A and B stored on the 0th, 2nd, 4th, 6th, and 8th days. This study is a descriptive study with a Quasi Experimental research design. Test cell A and test cell B were made from red blood cell specimens of 3 blood type A and 3 blood type B respectively. Test cells are stored in a refrigerator with a temperature of 2-6° C. Test cells were then examined on the 0th, 2nd, 4th, and 6th day of storage. Calculation of samples and repetitions using the Federer formula with the number of treatments in this study is 5 treatments. Based on the calculation, one sample of test cell A and test cell B was obtained with five repetitions of each examination. The results showed that on the 0th, 2nd, 4th, and 6th day of cell test storage, the results of blood type examination were obtained, namely the degree of agglutination 4+ with erythrocytes in the cell test clumping into one bond, cells forming large agglutination with clear supernatant. On the 8th day of storage, the result of agglutination degree is 3+ with erythrocytes in test cells not clumping perfectly, there are erythrocyte granules and cloudy supernatant. Based on the results of the study, it is concluded that test cell A and test cell B can be used optimally until day 6 storage.

Experiment Research of Superconducting Joint Resistance in Large-Scale HTS Magnet

Experiment Research of Superconducting Joint Resistance in Large-Scale HTS Magnet

Characteristics of constant pressure dehydrogenation and construction of linear diffusion model for solid hydrogen storage materials: A case study of TiMn1.5 alloy

Solid-state hydrogen storage is considered to be the most promising hydrogen storage technology because of its high volumetric capacity and good security. The process of hydrogenation and dehydrogenation of solid hydrogen storage materials can be characterized by a diffusion kinetic model, which can be used to guide the safe and efficient application of hydrogen storage materials. Currently, experimental study on the dehydrogenation characteristics under constant pressure outer boundary condition has not been carried out methodically, and a simple and reliable diffusion kinetic model under this condition should be established based on the experimental results. In this study, an experimental platform for hydrogen absorption and constant pressure dehydrogenation of solid hydrogen storage materials was independently established. The TiMn1.5 alloy and its interstitial hydrides were taken as the experimental research objects to conduct constant pressure dehydrogenation tests, and the dehydrogenation properties at different temperatures were systematically studied. The results show that the dehydrogenation rate decreases with the increase of testing time. The ultimate capacity of hydrogen release increases first and then decreases with the increasing temperature, reaching the maximum value at 30 °C. It is found that the diffusion coefficient increases linearly with time during constant pressure dehydrogenation process. On this basis, a linear diffusion kinetic model is established. The initial diffusion coefficient D0 calculated by the new model has a quadratic polynomial relationship with temperature, and the increase coefficient α of diffusion coefficient has a linear relationship with temperature. According to the mathematical expressions, the dehydrogenation process of TiMn1.5 alloy at different temperatures can be predicted. The research results can provide theoretical guidance for the safe, efficient and controllable application of hydrogen storage materials.

Assessment of the efficiency of functioning of the environmental management system of enterprises

Purpose. A multifactor model is developed to assess the efficiency level of an enterprise’s environmental management system, depending on the effectiveness of organizational environmental measures. Methodology. The authors’ method for determining a comprehensive criterion for monitoring the effectiveness of the environmental management system, which characterizes the efficiency of operation and determines the level of environmental safety of enterprises, is proposed and used. To solve the tasks, a complex research method was also used, which included the analysis and generalization of literary and patent sources, and analytical, experimental research using computer and mathematical modeling methods. Findings. According to the studies and calculations of the generalized environmental quality indicator – 0.64, 0.66 and 0.66, the largest negative impact on the environment is caused by the activities of enterprises in the Pecherskyi, Podilskyi, and Solomianskyi districts of Kyiv, respectively. The obtained data testify to the relationship between the effectiveness of the implemented environmental measures (saving and rational use of resources, application of environmental technologies, advanced training, and environmental competence of employees) and the level of efficiency of the environmental management system of enterprises. Originality. As a result of the studies conducted using actual data, a system of indicators of the generalized environmental quality indicator has been proposed for the first time, which allows determining the environmental efficiency and effectiveness of the implemented environmental measures to assess the effectiveness of the functioning of the implemented environmental management system of the enterprise. Practical value. Based on the research results, a technique is proposed to assess the effectiveness of the environmental management system of enterprises by determining a generalized environmental quality indicator in terms of reducing the negative impact of the enterprise’s activities on the environment. Such an assessment system will help the management of the enterprise to promptly introduce corrective actions to improve the efficiency of the environmental management system and increase the level of environmental safety.

2.F. Scientific session: Challenges in healthcare architectures

Abstract The Workshop titled “Challenges in healthcare architectures” - proposed and developed by the three EUPHA Section: Urban Public Health (URB), Public Mental Health (MEN) and Environmental Health (ENV) - is aimed to foster the dialogue between designers (architects and urban planners), Public Health experts (operators, professionals and epidemiologists), general/sanitary Directors of healthcare facilities and medical staff, to establish a multidisciplinary approach for understanding together how to create and manage the healthcare facilities of the future. According to the main Conference topic “Sailing the waves of European public health: exploring a sea of innovation”, the workshop mainly addresses both the “Health care services and systems” and the “Health workforce, practice and training” EPH24 conference topics. The Workshops purpose is to explore the concept for the hospital of the future, that should be resilient to changes and capable of protecting different users’ health and tackle the transforming social, economic, environmental and epidemiological needs of the context in which they are located. As in many other spheres and economic activities, the pandemic accelerated the processes of innovation and digitization that had somehow already been activated. The healthcare issue, the use of smart devices for a dialogue between the healthcare professionals and the pervasive use of apps to monitor certain health parameters, are just some of the megatrends that were being observed and which have now necessarily entered into everyday life. In the same way, the hospital, which in the face of this gradual process of dematerialization and relocation of ‘softer’ clinical-diagnostic activities, reaffirms itself as a center of the highest specialty for the treatment and care of acute patients, for high-level clinical and experimental re-search, for diagnostics and more complex operations. At the same time, and finally, the hospital is inserted in a territorial healthcare facilities network, that should be planned, designed, and managed. The workshop program includes inputs which they argue current experiences, emerging practices and scientific outcomes related to the hospital design, planning and management sphere, coming from four different European countries. From designing to managing practices, in “Hospital Design and planning in the line of Foresight of Health Needs” and in “Sustainability in the Next Generation Hospital”. From digitalization in national resilience experiences, in “Digital Health Spaces in the City of Lisbon. The Role of Digitalization in linking Boundaryless Hospitals and Smart Health Cities” to novel approaches for specific disease, in “Building bridges: mobile mental health care units in four European countries”. Key messages • Explore the “hospital of the future” concept, resilient to changes, capable of protecting users’ health, and to tackle the transforming social, economic, environmental and epidemiological needs. • Establish a multidisciplinary and territorial approach, for understanding together how to create and manage the healthcare network of the future.

Efficient Removal of Brilliant Cresyl Blue from Solution Using Inula Racemosa: Experimental Insights, DFT Modeling, and Docking Simulation

AbstractThis study examined the efficacy of Inula Racemosa leaves in the adsorption of Brilliant Cresyl Blue (BCB) from aqueous solutions. The study uses both experimental and theoretical research, including Density Functional Theory (DFT), to gain a better understanding of how the adsorbent and Brilliant Cresyl Blue molecules interact with each other. We determined the point zero value of the charged powder of Inula Racemosa and characterized the functional groups using FTIR spectroscopy. The experimental results indicate a significant ability of our adsorbent to adsorb the cationic dye Brilliant Cresyl Blue, and we have identified optimal conditions for its effective removal. The results suggest that the most effective amount of our biosorbent was 0.1 g. The equilibrium period for biosorption was 10 min, and the biosorbent capacity for biosorption improved with increasing pH. The Pseudo‐second‐order model accurately predicts the retention of Brilliant Cresyl Blue using Inula Racemosa. The Freundlich model was found to be better suitable for analyzing the isotherm data, revealing a maximum adsorption capacity of 3.49 mg g−1, achieving an efficiency removal of 89.95 %. We compared Density Functional Theory (DFT) calculations with experimental observations to validate the theoretical predictions and improve our understanding of the adsorption mechanism. Docking simulations show how the molecules of Inula Racemosa and Brilliant Cresyl Blue interact with each other, including the interactions of hydrogen bonds and electrostatic forces.

Topological electronic structure and electronic nematicity in candidate kagome superconductors, ATi3Bi5 (A = Rb, Cs)

Abstract The newly discovered family of titanium-based kagome metals, ATi₃Bi₅ (where A can be Rb or Cs), has been found to exhibit non-trivial band topology and fascinating electronic instabilities, including electronic nematicity and potential bulk superconductivity. Distinct from their vanadium-based counterparts (AV₃Sb₅), which display a charge density wave (CDW) phase that already breaks rotational symmetry, ATi₃Bi₅ shows no evidence of CDW, providing a unique platform to study nematicity in its pure form and its interplay with other correlated quantum phenomena, such as superconductivity. In this review, we highlight recent progress in both experimental and theoretical research on ATi₃Bi₅ and discuss the unresolved questions and challenges in this burgeoning field.

Study on recrystallization process of nitroguanidine by directly adding cold water to control temperature

Abstract Nitroguanidine (NGu), as a kind of high-energy material, is widely used as explosive propellant and energy component of explosives and smokeless powder. However, NGu crystals prepared by crystallization process are hollow and long needles, and their bulk density and bombing performance are greatly limited due to the high energy density at the tip, large porosity, and poor fluidity. Surprisingly, the particle size and morphology of NGu can effectively be improved by the recrystallization process. In this article, the recrystallization process by directly adding cold water to control temperature is first proposed, aiming to improve the morphological characteristics and reduce the particle size of NGu. Through single factor experimental research, the influences of various operating parameters on the morphology and particle size of NGu during recrystallization process were studied in detail. The results showed that the smallest average particle size of NGu was 19.8 μm when the crude NGu concentration, cold water temperature, volume ratio of boiling to cold water, stirring speed, and polyvinyl alcohol concentration were 5 g/100 mL, 0°C, 1:1, 800 rpm, and 0.35 g/100 mL, respectively. Correspondingly, the morphology of NGu was short rod, which was helpful to improve the bulk density. This novel recrystallization process has great potential to improve the NGu morphological characteristics, providing a new idea for the preparation of military NGu with small particle size and high bulk density.

The Effect of Using Interactive Learning Media on Science Learning Outcomes of Class IV Students at SD Negeri 136 Palembang

This research aims to determine whether there is an influence of the use of interactive learning media on the science learning outcomes of class VI students at SD Negeri 136 Palembang. This research is a quantitative type using the True Experimental type of experimental research method with a Matching Pretest – Posttest Comparison Group Design. The population of this study were all fourth grade students at SD Negeri 136 Palembang. The sample for this research was students in class IV.A and IV.B with a total of 43 students. The sample selection technique used was simple random sampling. Data collection techniques in this research are observation and tests. Data analysis used the Independent sample T-test. The results of this research show that the average posttest learning outcome score for the experimental class is 78.18 and the control class is 67.62. The results of the t-test calculation of learning outcomes obtained t_count= 2.460 ≥ t_tabel= 1.6829 with a significance level = 0.05. Based on the research results, it can be concluded that there is a significant influence of the use of interactive learning media on the science learning outcomes of class IV students at SD Negeri 136 Palembang.

Electron-positron pair creation under Gaussian and super-Gaussian pulse trains

The electron-positron pair (EPP) creation under Gaussian and super-Gaussian pulse trains are studied by the computational quantum field theory (CQFT) in the single-photon regime. The details of the EPP creation are studied from the time evolution of the EPP number, energy spectra and spatial distribution of the electrons. The results indicate that the final created EPPs is the non-linear accumulation of the multi-pulses, which depends on the time interval, pulse shape and pulse number. The optimal time interval can be chosen based on the pulse resonance condition, which is derived by the perturbation method. Besides, steeper super-Gaussian pulses and adding more pulses facilitate the EPP creation as well. The results indicate that, under optimal multi-pulse parameters, the number of the EPPs obtained is much larger than the sum of the EPPs created under the same number of single pulses. This finding not only can enhance the EPP creation, but also can improve the multi-pulse utilization and guide future experimental research on the EPP creation.

Study on the wear performance of 304 stainless steel aerospace joint bearings

In order to investigate the influence of surface micro-texture on the oil film carrying capacity, a theoretical model of dynamic pressure lubrication is constructed based on CFD, and dynamic pressure lubrication simulation and friction and wear experimental research are used to analyse the influence of speed and load on the friction behaviour of the bearing pair, and the wear performance of the specimen is evaluated by means of the surface morphology and friction coefficient. The results show that the carrying capacity of the oil film on the textured surface is relatively large compared with that of the non-textured surface, showing regular fluctuations, and that the pressure values of turbulent flow are greater than those of laminar flow. As the rotational speed increases, the cavitation effect becomes more and more obvious, and the carrying capacity of the oil film increases. The smaller the thickness of the surface oil film, the higher the load carrying capacity and the lower the degree of wear; The surfaces of the textured specimens showed the best friction reduction and anti-wear properties, followed by the smooth surfaces and the worst rough surfaces. With the increase of rotational speed, the friction coefficient tends to decrease. When the rotational speed is 0.4 m s−1, the wear of the textured surface is reduced. With the increase of load, the thickness of the formed oil film decreases, the friction coefficient decreases, and the anti-friction effect of the textured surface increases. This indicates that the surface texure treatment of 304 stainless steel, and the selection of appropriate working condition parameters can effectively reduce wear during the friction process, and improve the wear resistance of bearings.

Welding of Toroidal Cores Made out of Amorphous Alloy Using Capacitor Discharge Spud Welding Technology (CD Welding)

Amorphous alloys exhibit exceptional characteristics not typically associated with other known classes of materials. These alloys are industrially manufactured in the form of ribbons with thicknesses below 50 μm, which presents challenges in their welding process. Resistance spot welding utilizing energy stored in capacitors is employed for thin sheets or foils due to it’s precise energy control during capacitor discharge. This paper presents the findings from experimental research conducted on spot welding metallic amorphous ribbons with a thickness of 30 μm using stored energy in capacitors, aiming to establish effective welding technologies. Several tests with varying parameters were conducted to optimize the welding process and determine the most effective technology for this foil.

The Influence of the Talking Stick Learning Model on the Motivation and Learning Outcomes of Class V Students at SD Negeri 88 Palembang

This research aims to determine whether there is an influence of using the Talking Stick learning model on the motivation and learning outcomes of class V students at SD Negeri 88 Palembang. This research is a quantitative type using a Quasi Experimental type of experimental research method with a Nonequivalent Control Group Design. The population of this study were all fifth grade students at SD Negeri 88 Palembang. The sample for this research was students in classes V.3 and V.4 with a total of 50 students. The sample selection technique used was proportional sampling. Data collection techniques in this research are questionnaires and tests. Data analysis used the Independent sample T-test. The results of this research show that the average posttest learning motivation score for the experimental class is 87.28 and the control class is 78.72, while the average posttest learning result for the experimental class is 86 and the control class is 71.20. The results of the t-test calculation of learning motivation obtained t_count= 6.591 ≥ t_tabel= 1.678 and the results of the calculation of learning outcomes obtained t_count= 4.714 ≥ t_tabel= 1.678 with a significance level = 0.05. Based on the research results, it can be concluded that there is a significant influence of the use of the Talking Stick learning model on the motivation and learning outcomes of class V students at SD Negeri 88 Palembang.

Navigating the complexity of User Engagement: need for normative evaluations in digital interventions

Abstract Digital behavioral change interventions (DBCI), leveraging digital technology to promote cognitive behavioral therapy or positive psychology techniques, can potentially revolutionize health promotion. With applications across a spectrum of health behaviors-from managing common mental health disorders to enhancing physical activity, addressing smoking cessation, alcohol use, and weight management-these interventions offer promise for both healthy and clinical populations. However, their effectiveness hinges significantly on user engagement. Digital interventions are prone to attrition due to their self-help and unguided nature, which impedes their translation from research to real-life settings. This talk discusses the findings of a scoping review to shed light on these complexities. Firstly, we explore the various methodologies used to evaluate engagement across studies, hindering effective comparison and understanding. Secondly, we address the lack of systematic assessment of engagement strategies, advocating for experimental research to fill this gap. Lastly, we emphasize the need to establish an optimal level of engagement for desired outcomes, noting that continuous engagement may not always be conducive to behavioral change interventions, which aim to instill lasting changes in individuals. This underscores the importance of comprehensive assessment using both objective and subjective measures. In conclusion, engagement with digital interventions encompasses both quantitative usage metrics and qualitative subjective experiences, highlighting the need for comprehensive assessment beyond the mere evaluation of frequency or duration. Contrary to common belief, increased engagement does not always lead to better outcomes. Too much engagement can lead to exhaustion, while too little can result in inefficiency. Therefore, designing a DBCI that encourages optimal use is essential, fostering a balance that maximizes effectiveness while minimizing adverse effects.

AUDIT‐C shows reliability when used online in patient portals

The Alcohol Use Disorders Identification Test–Consumption (AUDIT‐C) is a validated screen, but until recently has not been evaluated in routine conditions for adult primary care patients. A new study has found that AUDIT‐C screens have “excellent” test‐retest reliability, which assesses the consistency of results when given at two different times. Excellent test‐retest reliability means that there are similar results across separate test administrations, and the study, supported by the National Institute on Alcohol Abuse and Alcoholism (NIAAA), shows that the AUDIT‐C can be used reliably in adult primary care patients. The study, led by Claire B. Simon, M.D., of the University of Washington in Seattle, used electronic health record (EHR) data from Kaiser Permanente. For the study, more than 18,000 adult primary care patients completed two AUDIT‐C screens at 1 to 21 days apart as part of routine care in 2021. Screen results were reliable whether completed in person or online through patient portals. However, reliability was higher when the patient completed both screens using online patient portals, compared to completing both screens in the clinic or one in the clinic and one online. Therefore, completing two screens may be a better option than just doing one. The study, High test–retest reliability of the Alcohol Use Disorders Identification Test–Consumption (AUDIT‐C) questionnaire completed by primary care patients in routine care, is published in the February 2024 issue of Alcohol, Clinical and Experimental Research. The researchers also found that reliability was lower for American Indian/Alaska Native (AI/AN) patients and multiracial patients.