Abstract Using two degrees of freedom (dof) experimental flutter derivatives to perform three-dimensional flutter analysis for a cable-supported bridge is a widely practiced method. It is important to consider the P-derivatives effect to have more accurate analysis for a long-span bridge. Through a case example, this paper studied some of the issues relating to the P-derivatives effects on flutter. The operational condition in two-dof experiments was discussed. It was suggested that due to the strong aeroelastic coupling effect of the sectional model studied in this research, there was an inherent weakness of two-dof experiments. The effect of the P-derivatives was studied for an example bridge by comparing the flutter analysis results using two-dof and three-dof experimental flutter derivatives.