Alternative and clean fuel usages are recognized as a crucial step in making air transportation environmentally-benign, efficient and sustainable. Conventional kerosene based jet fuel is mainly used in aviation sector and biofuel is selected as alternative and clean fuel for this investigation. This paper presents the exergoenvironmental analysis of a medium-size turboprop engine (m-TPE) fuelled by jet and biofuel for maximum operation mode (take-off) based on exergy and life cycle assessment (LCA) methodology. Environmental impact regarding the m-TPE and its subsystems is computed using Eco-indicator points per unit hour (mPts/h). Although the component-related environmental impact rate (29.235 mPts/h) of m-TPE is constant for jet and bio fuel utilization, the fuel consumption increases and the exergoenvironmental impact rate reduces by biofuel utilization. Besides, biofuel utilization decreases specific environmental impact of engine product from 5224.91 mPts/GJ to 1384.81 mPts/GJ due to the environmental impact points of fuel lessens from 240 mPts/kg for jet fuel to 55.80 mPts/GJ for biofuel. The results indicate that environmentally-friendly and clean fuel utilization in aero engines goes down environmental impacts although fuel consumption rises up to meet the energy needs of m-TPE. It is expected that the exergoenvironmental analysis method could help comparing m-TPE engines and different fuel utilization in terms of exergy based environmental impact. Additionally, this method can also be effective in making decisions about fuel selection of aero engines.