The growth of cleaner biodiesel generation associated with hydrodynamic cavitation from canola oil via transesterification is acquiring prominence owing to considerably descending energy needs and time. In this work, the conversion of canola oil into biodiesel and glycerol was achieved through the process of transesterification with methanol, employing sodium hydroxide as a catalyst. The newly offered process was investigated from technical, economic, and environmental points of view, and the precision of the results was verified and accepted compared to previously published works. The technical results revealed that the maximum exergy destruction rates as well as exergy efficiency belonged to the transesterification process. The economic study showed that the capital cost of the process was 638.7 MUSD/year. Also, the return period was computed less than 4 years. Moreover, comparison analysis proved that Canola oil proved to be a more advantageous primary material for the production of biodiesel and was more appropriate from the exergoeconomic point of view in comparison to Jatropha oil and other materials. Finally, by increasing the conversion percentage of the reactor, the value of the exergo-environmental index and the factor of exergy stability increased significantly, which was considered as an advantage from an environmental standpoint.