Excreta Moisture Research Articles

Effect of type of fiber and its physicochemical properties on performance, digestive transit time, and cecal fermentation in broilers from 1 to 23 d of age

The effects of insoluble fiber (IF) sources differing on particle size and hydration capacity (HC) on growth performance, gastrointestinal tract (GIT) development, cecal fermentation, and digestive transit time were investigated from 1 to 23 d of age in 550 Ross-308 broiler males housed in 50 cages. The experimental design was based on the administration of a common corn-soybean meal nonsupplemented with additional IF sources diet in mash form (Control), and 4 dietary treatments consisting in the Control diet diluted with 1.5% of different IF: lignocellulose (LC), fine-ground straw (FS) and coarse-ground straw (CS), all characterized by high HC; and almond shell (AS) having low HC. Cecal fermentation was assessed by in vitro fermentation of the IF sources using the cecal content of 22-day broilers as inoculum. Compared with control birds, the inclusion of IF sources tended to impair the FCR (P=0.053), with birds fed the HC-IF sources (LC, FS, and CS) showing lower ADFI (P=0.005) and ADG (P=0.001) than those fed the AS diet. The relative weight of gizzard and cecum, small intestine length, and digestive transit time decreased in AS group (P ≤ 0.050) compared with the average value of the groups fed the other IF sources. The inclusion of IF reduced (P=0.006) the excreta moisture content, with no differences among IF sources. Dietary treatments had no effect either on cecal short fatty acids concentration or on intestinal morphology. As indicated by the in vitro gas production results, both LC and AS were less fermented than FS and CS, although all IF sources were low fermentable. None of the IF sources were able to influence the fermentation capacity of cecal microbiota after 22 d of feeding. In summary, animals fed AS presented smaller gizzard and cecum relative size, showed higher ADFI and ADG, and tended to improve fed conversion ratio in comparison to those fed the rest of IF sources.

Kecernaan Kalsium dan Fosfor, pH gizzard, Kadar Air Ekskreta dan Digesta pada Ayam Petelur yang Diberi Ukuran Partikel Batu Kapur Berbeda dan Enzim Fitase

This study aimed to determine the coefficient of total calcium and phosphorus digestibility effects from limestone as a source of calcium and the effect of phytase enzymes on laying hens. This study used 180 Hy-line Brown laying hens aged 75 weeks. The experimental design used was a 2x2 factorial complete randomized design (CRD) with 9 replications. The first factor was limestone particle size, i.e., P1 = limestone flour and P2 = limestone granules, and the second factor was phytase enzyme dose, i.e., Q1 = 0 FTU and Q2 = 1000 FTU. The observed variables were gizzard pH, excreta moisture content (MC), digesta MC, calcium and phosphorus digestibility using two samples and ileal digestibility. The results showed that the digestibility of calcium from limestone flour was significantly different compared to limestone granules at P<0.05. The phytase addition and the interaction with limestone particle size did not affect gizzard pH, total and ileal digestibility of calcium and phosphorus. Excreta and digesta MC had a significant effect on the particle size of limestone, the highest MC was limestone granules compared to flour (P<0.001). The addition of phytase had an effect on reducing excreta MC (P< 0.001). There was interaction between limestone size and phytase in excreta and digesta MC. The conclusion of the research was that laying hens were more efficient in digesting calcium from limestone flour compared to limestone granules. Phytase could reduce excreta and digesta MC.
 Key words: calcium, digestibility, limestone, particle size, phytase

Quantitative and molecular aspects of water intake in meat-type chickens

Even though water is the most essential nutrient for poultry production, adequate data on individual water intake in broiler chickens and its relationship with other traits of economic importance is scant. Water is provided to chickens in an unrestricted manner in spite of being a finite resource. Climate change continues to affect water sources and efficient bird use of water is long overdue. Understanding the biological basis of water intake is essential for sustainability of the poultry industry. Individual water and feed intake, and growth data was collected on 520 commercial broilers aged 14 to 42 days. We introduced the concepts of water conversion ratio (WCR) and residual water intake (RWI) as parameters that can be used to assess water intake efficiency. Water conversion ratio was defined as the amount of water consumed per unit of body weight gain, and RWI was defined as the difference between the actual water intake (WI) of a given bird and the expected WI by an average bird from the population with the same metabolic body weight, feed intake (FI) and body weight gain (BWG). The correlation between WI and FI was positive (r=0.77; P<0.0001), and the correlation between WI and BWG was positive (r=0.80; P<0.0001). Based on the distribution of RWI, the bottom 5 birds (LRWI) and the top 5 birds (HRWI) for RWI were selected for mRNA expression differences. The average broiler consumed about 7.8 L (± 1L) of water from 14 to 42 days of age. The mRNA expression of arginine vasopressin (AVP) antidiuretic hormone, calcium sensing receptor (CasR), sodium channel epithelial 1 subunit alpha (SCNN1A) and SCNN1D in the hypothalamus was upregulated in the LRWI group compared to the HRWI group. Similarly, kidney aquaporins (AQP) 2, 3, and 4 were upregulated in the LRWI group compared with the HRWI group. Given that water was provided ad libitum, the up-regulation of AVP and AQP gene mRNA expressions seem to indicate that the LRWI birds were more efficient in water reabsorption in the kidney compared to their HRWI counterparts. Increased water reabsorption will reduce the amount of water consumed to attain hydration. The water reabsorption potential was reflected in the excreta moisture levels as the LRWI birds had significantly lower excreta moisture than the HRWI birds. Excreta moisture level require further studies and could be considered as a potential proxy trait for water intake.

Indicators of thermal comfort and nitrogen digestibility as a function of digestible arginine: lysine ratios in the diet of laying Japanese quails raised in hot weather

The objective with this study was to determine the influence of the digestible arginine: lysine ratio in the diet of laying Japanese quails raised in hot weather on the physiological variables of thermoregulation, time in tonic immobility, behavior and nitrogen utilization. A total of 240 laying quails (Coturnix coturnix japonica) with 40 weeks of age were used in a completely randomized design with five treatments and six replications containing eight quails per experimental unit. The studied digestible arginine: lysine ratios were 110%; 120%, 130%, 140% and 150%, for 63 days divided into three periods of 21 days each. There was a quadratic effect (p < 0.05) for the variables average surface temperature, average body temperature, core to surface thermal gradient, ambient to surface temperature gradient observed in the morning. In the afternoon, a quadratic effect (p < 0.05) of the arginine: lysine ratio was observed for variables average surface temperature, average body temperature, core to surface thermal gradient, ambient to surface thermal gradient, back temperature, head temperature and respiratory frequency. There was no significant effect (p > 0.05) of arginine: lysine ratios on the frequency of behaviors, except drinking and panting (p < 0.05), for which 130% ratio provided the lowest frequencies of these behaviors. There was a quadratic effect (p < 0.05) for the parameters of nitrogen retention, nitrogen excreted and nitrogen retention efficiency. Nitrogen intake and excreta moisture were not influenced by the different relationships studied (p > 0.05). The 130% arginine: lysine ratio corresponding to 0.131 g/kg/feed of arginine improves physiological responses related to thermoregulation and decreases the expression of behaviors correlated to heat stress, also meeting the best estimated ratio for nitrogen retention in the diet of quails laying Japanese raised in hot weather.

Assessments of productive performance, eggshell quality, excreta moisture, and incubation traits of laying breeder hens fed a proprietary blend of Quillaja and Yucca.

A study was conducted to evaluate performance, eggshell quality, nutrient metabolizability, and incubation traits of laying breeder hens fed diets supplemented with an additive containing polyphenols and saponins of a proprietary blend from Quillaja saponaria and Yucca schidigera (QY) biomass. Hens were fed 4 feeds in 5 periods of 28 days each from 30 to 49 weeks of age. Experimental feeds were a Control diet; Control + virginiamycin (33 g/ton); Control + QY (250 g/ton) and Control + virginiamycin + QY. A total of 40 White Plymouth Rock and 44 Rhode Island Red breeder hens were allocated in individual cages using a completely randomized block design with 21 replicates. Performance parameters, evaluated per period, were egg production, egg weight, FCR, egg mass, and culled eggs. All eggs were collected in the last 4 days of each period to evaluate specific egg weight, percentage of albumen, yolk and shell, and Haugh unit as well as cuticle quality, shell strength, and shell thickness. At the end of the experiment, nutrient metabolizability assessment and four incubations were conducted. There were no interactions between diet and period in all evaluated responses (P > 0.05). Experimental diets did not affect daily egg production, egg weight, and egg mass as well as Haugh unit, yolk and albumen percentage, and yolk color (P > 0.05). However, hens fed Control + QY produced eggs with better shell strength, shell thickness, and cuticle quality than hens fed the Control (P < 0.05). Hens fed Control + QY or Control + virginiamycin + QY had lower culled eggs, better FCR and higher egg specific weight, shell percentage, and yolk strength compared to breeder hens fed the Control (P < 0.05). In general, hens fed QY achieved enhanced performance and egg quality compared to virginiamycin. In conclusion, laying breeder hens fed diets supplemented with Quillaja and Yucca additive, from 30 to 49 weeks of age, maintained their productive performance, had improved eggshell and cuticle quality and reduced culled, dirty and contaminated eggs.

Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks

The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers. Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch. These 5 treatments were administered to 10 replicate cages of 8 birds. Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d. At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage. Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically. Feed conversion ratio increased (P < 0.01) linearly as GOS increased. There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn. There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased. At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased. Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS. Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium. Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.

Effect of graded dietary inclusion levels of hybrid rye grain on productive performance, the cost-effectiveness of nutrition and egg quality in laying hens

Abstract The study was designed to investigate productivity parameters, egg quality and the cost effectiveness of feeding laying hens with diets where ground wheat was replaced with varying levels of whole hybrid rye grain cv. Brasetto. A total of 396 Hy-Line Brown hens at 34 weeks of age were allocated to three treatment groups with 66 replicates (cages) of two hens each, and were fed isocaloric and isonitrogenous pelleted diets for 12 weeks. The diet for control group hens (R0) did not contain rye grain. In diets for hens from groups R100 and R200, ground wheat grain was partially replaced with whole rye grain at 100 g/kg and 200 g/kg, respectively. Whole intact rye grains were included in the diets. The cost of diets containing 10% and 20% of whole rye grain was by approximately 1.0% and 1.9% lower, respectively, compared with the control diet that contained no rye grain. During the entire 12-week feeding period, dietary treatments had no effect on laying rate (95.64% to 97.07%), egg weight (62.51 g to 62.74 g) or egg mass output (5.04 kg to 5.12 kg per hen). The inclusion of whole rye grain in layer diets caused a linear increase in feed intake (P&lt;0.047) and tended to linearly increase the feed conversion ratio (FCR, P = 0.078). Both groups of hens fed diets containing whole rye grain were characterized by a linear decrease in the dry matter (DM) content of excreta (P = 0.001). Increased excreta moisture was accompanied by a tendency towards a higher incidence of dirty eggshells in R200 hens (P = 0.068). The inclusion of 100 g/kg of whole rye grain in layer diets decreased the cost of feed per kg of eggs by 1.9%. Dietary treatments had no significant effect on eggshell thickness (0.330 mm–0.345 mm), albumen quality (Haugh units, 83.46–85.84) or yolk color (3.8–4.0). The results of this experiment indicate that pre-pellet inclusion of whole rye (at up to 200 g/kg) in diets with supplemental NSP-degrading enzymes and phytase can be an effective and economically viable alternative to ground wheat in pelleted layer diets.

Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens

ABSTRACT 1. The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens. Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured. 2. Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg). Birds were fed the dietary treatments for 56 d. 3. Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05). Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058). 4. Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp. and isobutyric and succinic acid, and ileal concentration of Bacillus sp. and total anaerobic bacteria (P < 0.05). Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp. and valeric acid (P < 0.05). 5. These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.

The Impact of Bacillus subtilis PB6 and Chromium Propionate on the Performance, Egg Quality and Nutrient Metabolizability of Layer Breeders.

Simple SummaryEnvironmental conditions can generate heat stress in poultry production. Feed additives have been supplemented in diets for layers and broilers to improve their health status, immune system and nutrition, as well as productive performance. This paper shows the effects of probiotic Bacillus subtilis PB6 and chromium propionate diet supplementation on productive parameters, egg and eggshell quality, excreta moisture, cortisol and serum biochemistry of laying breeder hens. Diets supplemented with Bacillus subtilis PB6, chromium propionate or a combination of the two resulted in improved egg production, feed conversion ratio, eggshell quality and nutrient metabolizability, without modifying the main serum biochemical parameters of hens from 55 to 70 weeks of age. Highlighted improvements in hen performance and eggshell quality were observed when hens were fed diets supplemented with a combination of the probiotic and chromium. This study expands our understanding concerning the combined supplementation of probiotics and organic chromium for poultry.The objective of this study was to evaluate the effects of Bacillus subtillis PB6, chromium propionate or a combination of the two on the performance, egg and eggshell quality, nutrient metabolizability and serum biochemistry of layer breeders. White Plymouth Rock and Red Rhodes Island breeder hens at 55 weeks of age were allocated in individual cages using a completely randomized block design with 16 replicates. Hens were fed control, control + probiotic (500 g/ton of Bacillus subtilis PB6), control + CrProp (50 g/ton of chromium propionate) and control + probiotic + CrProp diets from 55 to 70 weeks of age. Productive parameters and eggshell quality as well as cortisol and blood biochemistry were grouped each 28 d as well as for the overall period. The metabolizability of nutrients and energy was determined at 70 weeks of age. In the overall period, hens fed the control + probiotic or control + probiotic + CrProp diets had significantly higher egg production, egg mass, shell percentage, thickness and shell strength. The metabolizability of dry matter, nitrogen and energy increased in hens that were fed the control + probiotic + CrProp diet. In conclusion, diets supplemented with Bacillus subtillis PB6 and chromium propionate resulted in improved productive performance, eggshell quality and nutrient metabolizability of layer breeders, without modifying serum cortisol, albumin and triglycerides.

Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers

ABSTRACT 1. The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens. 2. Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high). Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet. The diets were fed in three phases, starter (d 0–12), grower (d 12–23) and finisher (d 23–31). 3. For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen. Dry matter (DM) content was measured in the excreta and litter samples. Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated. 4. In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0–23 compared to low sNSP (P = 0.004 and P = 0.044, respectively). In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008). For all diets, degradability of sNSP was higher and insoluble NSP was lower when feeding low compared to high sNSP (P < 0.001). In birds fed the barley- and sorghum-based diets, AMEn was lower when feeding the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively). 6. Results from this study showed that level of dietary sNSP impacts broiler productive performance and nutrient utilisation.

Impact of fiber on growth, plasma, gastrointestinal and excreta attributes in broiler chickens and turkey poults fed corn- or wheat-based diets with or without multienzyme supplement

Effects of fiber on growth performance, gizzard attributes, ileal digesta viscosity, plasma uric acid (PUA) and excreta characteristics were investigated in broiler chickens (experiment 1) and turkey poults (experiment 2) fed corn or wheat-based diets with or without multienzyme supplement (MES). Fibrous diets were created by adding 10% corn distillers dried grains with solubles or wheat middlings in corn or wheat-based diets, respectively. The MES had main activities of xylanase and β-glucanase. A total of 960-d old Ross x Ross 708 male chicks and 720-d old male Hybrid toms were allocated to eight grain, fiber and MES combinations to give 6 replicates per combination. In each experiment, birds had free access to feed and water for 28 days. Excreta samples were collected for 3-d prior to the end and on d 28, body weight and feed intake were recorded, birds bled and subsequently necropsied for gastrointestinal samples. There was an interaction (P ≤ 0.036) between grain, fiber and MES in broilers final body weight (FBW) and BW gain (BWG). In this context, high fiber corn diets reduced FBW and BWG and supplementation of MES improved these parameters. Broilers fed corn had a higher (P < 0.05) FBW (1,462 vs. 1,424 g) and BWG (1,416 vs. 1,378 g) than birds fed wheat diets. Broilers fed corn-based diets without fiber diets had a higher ileal viscosity and excreta moisture compared to birds fed wheat-based and high fiber diets. Broilers fed low fiber wheat diets without MES had higher (P < 0.05) PUA concentration compared to birds fed low fiber corn diets without MES. Poults fed wheat diets had a higher (P < 0.05) FBW (1,441 vs. 1,408 g) and BWG (1,376 vs. 1,343 g) than poults fed corn diet. The MES supplementation in corn-based diets rich in fiber increased (P = 0.03) gizzard weight in poults. In conclusion, there were varied growth and physiological responses in broilers and turkey suggesting the need for refining enzyme application for different poultry species.

The effect of different dietary levels of sodium and chloride on performance, blood parameters and excreta quality in goslings at 29 to 70 days of age.

The purpose of this study was to ascertain the appropriate levels of dietary sodium (Na+ ) and chloride (Cl- ) for 29- to 70-day-old goslings and to investigate the effects of different levels of Na+ and Cl- on the growth performance, water consumption, blood parameters and excreta quality of goslings to provide a reference for the healthy production of goslings. In Experiment 1, a total of 432 29-day-old male Jiangnan White goslings were randomly allotted to nine treatments according to a 3×3 factorial design, with six pens containing eight birds per treatment. The goslings were fed diets with three concentrations of Na+ (0.10%, 0.15% and 0.20%) and three concentrations of Cl- (0.15%, 0.20% and 0.25%). The experimental period was 42days. In Experiment 2, a total of 24 70-day-old Jiangnan White goslings were selected for four treatments (0.10% Na+ ×0.15% Cl- ; 0.10% Na+ ×0.25% Cl- ; 0.20% Na+ ×0.15% Cl- and 0.20% Na+ ×0.25% Cl- ) and housed separately in metabolic cages. The faeces were collected for 3 consecutive days. In Experiment 1, the average daily feed intake (ADFI), average daily gain (ADG) and feed/gain (F/G) ratio of goslings were unaffected by the treatments. However, low levels of Na+ and Cl- significantly reduced the water consumption of goslings in the later growth period (p<0.05). The average water consumption of goslings fed with 0.10% Na+ ×0.15% Cl- was significantly lower than that of the goslings fed with 0.20% Na+ ×0.25% Cl- (56days, 1304.2ml vs. 1471.7ml; 63days, 1452.8ml vs. 1610.8ml; 70days, 1540.0ml vs. 1775.4ml; p<0.05). The interaction between Na+ and Cl- (Na+ ×Cl- ) had a significant impact on the blood haemoglobin (HGB) and haematocrit (HCT) levels in the goslings (p<0.05). HGB increased linearly with increasing levels of Na+ . HGB and HCT first increased and then decreased with increasing levels of Cl- . In Experiment 2, Na+ and Cl- levels had significant effects on the excreta moisture content (p<0.05). Goslings fed with 0.10% Na+ ×0.15% Cl- had a low moisture content of 5.58% compared to the goslings fed with 0.20% Na+ ×0.25% Cl- (87.51% vs. 93.09%; p<0.05). The levels of dietary Na+ had a significant effect on the retention ratio of Na (p<0.05), with the value for the 0.20% Na+ group being significantly higher than that for the 0.10% Na+ group (p<0.05). In summary, different levels of Na+ and Cl- did not affect the growth of goslings. To reduce the water consumption and moisture content of excreta, the Na+ and Cl- levels in the diet can be as low as 0.10% and 0.15%, respectively.

Inclusion of kaolin in the diet of brown egg layers at the end of the production cycle

ABSTRACT: This study investigated the inclusion of kaolin levels in the diet of brown egg layers at the end of the production cycle. A completely randomized design involved the distribution of 288 brown egg layers into six treatments of eight replications, with six birds per replication. When the birds reached the age of 63 weeks, the feeding of the experimental diets began. Diets differed by the inclusion of increasing levels of kaolin: Control (no additive), and the inclusion of 1%, 2%, 3%, 4%, and 5% kaolin. The performance, egg quality, intestinal morphometry, and excreta moisture were evaluated. The inclusion of kaolin promoted improvement in the jejunum villus height, enabling less feed intake; however, there were no significant differences in egg quality. The excreta moisture decreased linearly as the kaolin level in the diet increased. The inclusion of 5% kaolin in the diet of brown egg layers at the end of the production cycle improved intestinal morphometry and the quality of excreta through a reduction of moisture.

Aluminossilicatos na alimentação de aves: revisão de literatura

Os aluminossilicatos são comumente utilizados como aditivos tecnológicos na avicultura industrial pela sua ação como aglutinantes de rações e como adsorventes de micotoxina, no entanto quando fornecidos em maiores quantidades, podem atuar em diversas outras funções fisiológicas da ave, traduzindo em ganhos produtivos. Portanto o objetivo dessa revisão bibliográfica é elucidar sobre a ação de diferentes aluminossilicatos, e a possibilidade da utilização dos mesmos na nutrição de aves de interesse zootécnico. Com o aumento do uso de rações peletizadas nas indústrias os aluminossilicatos podem ser usados, aumentando a durabilidade e qualidade dos pellets, através da absorção da umidade. Devido à presença de cargas elétricas, esses aditivos possuem capacidade de ligação com cerca de 86% a 97% da quantidade de micotoxinas presentes nas rações, adsorvendo-as. Essa mesma propriedade os permite se ligar à toxinas e bactérias do intestino, e sua habilidade de absorver água, impacta significativamente na redução da umidade das excretas. Todas essas características podem resultar em melhor desempenho, bem-estar e qualidade dos produtos avícolas. No entanto devido à essa presença de cargas os aluminossilicatos podem adsorver vitaminas, minerais e outros aditivos presentes nas rações. Portanto o uso de aluminossilicatos é uma alternativa viável em rações de frangos de corte, galinhas, codornas e perus, no entanto, mais estudos devem ser realizados a fim de determinar quais desses minerais podem ser usados e em quais concentrações.

The effect of NSP-degrading enzymes on gut physiology and growth performance of turkeys fed soybean meal and peas-based diets

The aim of this study was to determine nutrient digestibility, short chain fatty acids (SCFA) production, bacterial enzyme activities and production performance effects of peas as an alternative dietary protein source. Diets containing peas were fed to growing turkeys for 8 weeks. The experimental design involved two diets, a wheat-soybean meal (SBM) diet and a wheat-SBM-peas diet, each with and without the addition of NSP-degrading enzymes. Peas were added at 300 g/kg at the expense of wheat and SBM. Nutrient digestibility, energy metabolizability and nitrogen retention remained unchanged in all groups. The SBM and peas diets did not affect the final body weights (BW) of birds, but the peas diet significantly (P < 0.05) increased FCR relative to the SBM diet (1.80 vs. 1.74 kg/kg). Enzyme supplementation significantly increased the final BW of turkeys (3.88 vs. 3.75 kg), irrespective of the diet type. A diet by enzyme interaction revealed that during the first 4 weeks of feeding, the enzyme preparation improved feed utilization in the SBM diet but not in the peas diet (1.37 vs. 1.44 kg feed/kg BWG). Enzyme supplementation decreased apparent ileal viscosity (1.60 vs. 1.82 mPas). Cecal ammonia concentrations and the activity of bacterial β-glucosidase, β-xylosidase, and α-arabinofuranosidase decreased significantly in response to peas diets, irrespective of enzyme supplementation. Enzyme supplementation significantly decreased β-glucosidase activity and the SCFA pool in the ceca, regardless of diet type. A diet by enzyme interaction revealed that the NSP-degrading enzyme supplements significantly decreased cecal SCFA concentrations but only in the peas diet (163 vs. 221 μmol/g). Excreta moisture content was significantly lower in the peas diet, regardless of enzyme supplementation and diet type. The Foot Pad Dermatitis (PFD) score was considerably (P < 0.05) reduced in the peas diet (1.43 vs. 1.53–1.60 in the remaining groups). In conclusion, peas could be effectively used in the diets of young growing turkeys at 300 g/kg at the expense of wheat and SBM without any negative effects on the gastrointestinal function and final BW. However, higher FCR values were noted for the peas diet. The benefits of the NSP-degrading enzymes included a decrease in ileal viscosity and excreta moisture content, an increase in cecal butyrate production, and an increase in the final BW of turkeys.

Addition of fermented and unfermented grape skin in broilers’ diets: effect on digestion, growth performance, intestinal microbiota and oxidative stability of meat

Grape skin is a source of polyphenols with antioxidant and antimicrobial properties. Little information is available regarding its application in animal feeding. The present study investigated the effect of inclusion of fermented (FS) and unfermented (UFS) grape skin at two different doses (30 g/kg, FS30 and UFS30, and 60 g/kg, FS60 and UFS60) and 200 mg/kg vitamin E (α-tocopheryl acetate) in a corn–soybean diet on growth performance, ileal protein digestibility, ileal and excreta total extractable polyphenols content and digestibility, intestinal microbiota and thigh meat oxidation in broiler chickens. Growth performance was depressed in chickens fed UFS and FS diets. A reduction in ileal protein digestibility was also observed in birds fed UFS, being this effect more pronounced in those fed 60 g/kg. The dietary inclusion of grape skin increased both ileal and excreta polyphenols contents, being higher in birds fed UFS than in those fed FS. Excreta moisture content increased in birds fed UFS and FS diets. No effect of dietary inclusion of grape skin was observed on ileal counts of lactic-acid bacteria and Clostridium, but UFS inclusion in the diet reduced ileal count of Escherichia coli as compared with FS dietary inclusion. After 7 days of refrigerated storage, values of thiobarbituric acid reactive substances (TBARS) were lower in chicken meat when grape skin was added in the diet at 60 g/kg instead of 30 g/kg, and meat from birds fed 60 g/kg of grape skin reached TBARS values similar to those of birds supplemented with vitamin E. In conclusion, high doses of grape skin polyphenols depressed growth performance and protein digestibility, and increased excreta moisture content. Unfermented grape skin contained more polyphenols than FS, and its inclusion in the diet led to higher ileal and excreta polyphenols contents and to a lower ileal count of E. coli. Furthermore, the antioxidant potential of the polyphenols present in grape skin was observed after 7 days of meat storage, with the dose of 60 g/kg of grape skin being as effective as vitamin E supplementation in maintaining oxidative stability of meat.

Fiber levels in laying quail diets.

The objective of this work was to determine the effect of inclusion levels of dietary fiber in the Japanese quail diets. 480 Japanese quail were distributed in a completely randomized design, with 5 treatments and 12 replicates composed of 8 birds each. The treatments were constituted by five increasing levels of fiber in the diet: 2.78; 3.08; 3.38; 3.68; 3.98%. The parameters of productive performance, water consumption, egg quality, total lipids in the egg, excreta humidity, weight of digestive organs and intestinal morphology were evaluated. There was no significant effect of the use of different fiber levels on feed intake, egg production, viability, live weight, relative and absolute weight of gizzard, small intestine and cecum, and in excreta moisture of Japanese quais. Higher dietary fiber levels resulted in improvements in feed conversion per mass and per dozen eggs and higher water consumption. There was no significant difference for egg quality, except fot the color of the yolk where it was observed higher pigmentation of this structure with higher levels of fiber inclusion, and the lipid concentration in the buds was influenced in a quadratic manner. The inclusion of fiber promoted improvement in the villi of the duodenum and cecum.

Saltbush (Atriplex nummularia and A. amnicola) as potential plants for free-range layer farms: consequences for layer performance, egg sensory qualities, and excreta moisture

Highly adapted plant species suited to low rainfall conditions need to be considered for free-range poultry farms, particularly in marginal rainfall areas. A group of suitable plants are the saltbushes, in particular old man saltbush (Atriplex nummularia) can provide shelter, shade, and possibly forage. The first experiment examined whether hens eat old man saltbush while ranging. Free-range Hy-Line Brown layer hens were provided saltbush or "no" saltbush on the outdoor range throughout 11 wk of early production (16 to 27 wk). The quantity of saltbush eaten by the hens was determined by the n-alkane method. Hen interactions with saltbush were video recorded, and hen live weight, feed intake, egg production, and egg quality were measured. Hens ate the saltbush at 5% of their dietary dry matter intake. This level of saltbush intake had no influence on egg production. The video footage revealed hens actively pecked at the saltbush. The second experiment investigated the consequences of hens diluting their diet by eating increased levels of River saltbush (Atriplex amnicola). Air-dried, hammer-milled river saltbush was mixed and pelleted into a standard commercial layer diet at the following levels; 0 (control), 5, 10, 15, and 20%. Hy-Line Brown layer hens were fed the diets for 28 D (32 to 35 wk of age). Seventy-five hens were housed in individual cages, with 15 hen replicates per diet. The saltbush had no significant impact on egg production, hen live weight, or feed intake. Excreta moisture increased significantly (P < 0.001) with increased saltbush (5, 10, 15, and 20%) in the diet. Furthermore, high saltbush eggs (20%) had significantly stronger egg yolk color (P = 0.006) and thicker egg shells (P < 0.001) compared to the control eggs. Consumers were presented with the eggs from the second experiment and they preferred the high saltbush eggs (20%). In conclusion, saltbush would be a valuable plant on free-range poultry farms to provide shade and shelter, especially in those areas with marginal rainfall.

High dietary zinc and glutamine do not improve the performance or reduce excreta moisture of broiler chickens fed diets with and without magnesium supplementation.

An experiment was conducted to investigate the effect of supplemental L-glutamine (L-Gln) and a higher concentration of zinc (Zn) on excreta moisture under nutritionally induced wet droppings via decreased intestinal water reabsorption. A 2 × 2 × 2 factorial arrangement of treatments was used to investigate 3 dietary factors of L-Gln supplementation (0 or 10g/kg), and added Zn concentration (80 and 160mg/kg) with or without magnesium chloride (MgCl) (2g/kg-only in grower diets). A total of 576 male day-old Ross 308 broiler chickens were assigned to the experimental diets. Each diet was replicated 6 times with 12 birds per replicate. Wheat-based diets were formulated to be isocaloric and isonitrogenous. Starter diets were given from day 0 to 9 followed by grower (day 10 to 23) and finisher diets (day 24 to 35). Excreta moisture was measured for all the growth phases. The moisture content of different segments of intestine was assessed for starter and grower phases of feeding. There was no significant effect of any of the 3 main treatments on body weight gain or feed intake of birds during the experiment. Birds fed higher Zn (160mg/kg) tended (P = 0.09) to have higher weight gain only in the first 9 days of age. Feeding 10g/kg L-Gln increased the feed conversion ratio of the birds only from hatch until day 9 after which there was no significant effect. No effect of experimental treatments was found on digesta or excreta moisture, except a reduction in ileal moisture at the starter phase resulting from higher Zn concentration in the diets. MgCl at 2g/kg was not effective in inducing wet droppings in birds fed grower diets. Under the conditions of this study, no positive response was observed in terms of performance or reduction in excreta moisture when birds were fed diets containing 10g/kg L-Gln or higher concentration of Zn.

Growth performance, apparent retention of components, and excreta dry matter content in Shaver White pullets (5 to 16 week of age) in response to dietary supplementation of graded levels of a single strain Bacillus subtilis probiotic.

Administered in adequate amounts, probiotics can be an alternative to antibiotic growth promoters in poultry production. This study evaluated dose response of a single strain of Bacillus subtilis (SSB, DSM29784) on growth performance, apparent retention (AR) of components, and excreta DM content in pullets. A basal corn-soybean meal diet was formulated to meet the specifications for grower (week 5 to 10) and developer (week 11 to 16) phases. In each phase, SSB was added to the basal diet to create 4 test diets: 0 (control, CON), 1.1E+08 (low; LSSB), 2.2E+08 (medium; MSSB), or 1.1E+09 (high; HSSB) CFU of SSB/kg of feed. All diets had TiO2 (0.5%) as a digestibility marker. A total of 720 day-old Shaver White chicks were placed in 48 cages (15 pullets per cage) and reared on a commercial antibiotic free diet for a 4-wk period. At the beginning of week 5, treatments were allocated based on cage BW (n = 12). Birds had free access to feed and water throughout. The BW, BW uniformity, feed intake (FI) and FCR were obtained weekly. Excreta was collected at the end of each phase for AR of DM, organic matter, CP, neutral detergent fiber, minerals, and AME, as well as excreta DM content. In response to SSB inclusion, BW improved in a linear (P < 0.005) and quadratic (P < 0.0001) manner in grower. FI decreased in a linear and quadratic pattern (P < 0.05, week 8 to 10) in grower, and linearly (P < 0.05) across the developer phase. Overall FCR improved in linear and quadratic pattern (P < 0.01) in the grower phase. In both the phases, AME improved in a quadratic pattern (P < 0.05). Inclusion of SSB had a linear reduction (P < 0.0001) in excreta moisture content in the grower phase. In summary, these results demonstrate that B. subtilis probiotic improved performance through enhanced nutrient utilization and reduced excreta moisture content indicating improved pullet gut health.