Exclusion Zone Research Articles

Cytotoxic Flavokawain B Inhibits the Growth and Metastasis of Hepatocellular Carcinoma through UCK2 Modulation of the STAT3/Hif-1α/VEGF Signalling Pathway.

Hepatocellular carcinoma (HCC) is associated with a high mortality rate due to early recurrence and its metastasis features. To this day, effective treatment options for metastatic HCC remain a major challenge to patient treatment. Flavokawain B (FKB) is a naturally occurring chalcone molecule capable of providing effective therapy against this life-threatening disease. This study investigated the anti-metastatic effects of FKB on the growth and development of metastatic HCC. HepG2 cells were used in this study and a neutral red assay was performed to determine the IC50 value of FKB. Cell scratch and exclusion zone assays were performed to assess the rate of cell migration and invasion. Relative mRNA levels of UCK2, STAT3, VEGF and HIF-1α genes were quantified using RT-qPCR. FKB inhibited the proliferation of HepG2 cells at an IC50 value of 28 μM after 72 h of incubation. Its cytotoxic effect was confirmed to induce apoptosis through the phase-contrast inverted microscope. Cell migration and invasion were significantly inhibited at 7, 14, and 28 μM of FKB as compared to untreated cells. The inhibition in the cell migration significantly increased with the increasing concentrations of the bioactive compound. The relative expression levels of the UCK2 gene and its downstream genes, STAT3, VEGF and HIF-1α, were significantly downregulated after 72 h exposure to FKB treatment. Our data suggest that FKB inhibited HepG2 proliferation and further suppressed its metastasis partly by regulating the STAT3/Hif-1α/VEGF signalling pathway. FKB could be a potential alternative and viable strategy against HCC.

Population transcriptogenomics highlights impaired metabolism and small population sizes in tree frogs living in the Chernobyl Exclusion Zone

BackgroundIndividual functional modifications shape the ability of wildlife populations to cope with anthropogenic environmental changes. But instead of adaptive response, human-altered environments can generate a succession of deleterious functional changes leading to the extinction of the population. To study how persistent anthropogenic changes impacted local species’ population status, we characterised population structure, genetic diversity and individual response of gene expression in the tree frog Hyla orientalis along a gradient of radioactive contamination around the Chernobyl nuclear power plant.ResultsWe detected lower effective population size in populations most exposed to ionizing radiation in the Chernobyl Exclusion Zone that is not compensated by migrations from surrounding areas. We also highlighted a decreased body condition of frogs living in the most contaminated area, a distinctive transcriptomics signature and stop-gained mutations in genes involved in energy metabolism. While the association with dose will remain correlational until further experiments, a body of evidence suggests the direct or indirect involvement of radiation exposure in these changes.ConclusionsDespite ongoing migration and lower total dose rates absorbed than at the time of the accident, our results demonstrate that Hyla orientalis specimens living in the Chernobyl Exclusion Zone are still undergoing deleterious changes, emphasizing the long-term impacts of the nuclear disaster.

Freshwater gastropods as pathogens of natural focal invasions in the aquatic ecosystems of the Chernobyl exclusion zone

It has been established that 20 species (including two species complexes) of freshwater gastropods from 7 families live in water bodies on the territory of the PSRER, in which 51 species of trematodes from 21 families with a predominance of species of the Echinostomatidae family (10 species) parasitize at the parthenitis stage. The maximum number of trematode species was registered in the mollusks Lithoglyphus naticoides and Planorbarius corneus – 8 species of parasites with an infestation of 11.0 and 28.4 %, respectively. In the exclusion zone of the Chernobyl NPP, the circulation of natural focal helminthiasis caused by parasitization of trematodes Opisthorchiidae (Metorchis bilis, Pseudamphistomum truncatum, Opisthorchis felineus) identified in the studied animal species was established. For the mollusk Bithynia leachii, a high degree of infection (43.1 %) with the trematode O. felineus was noted.

Evaluating how well active fault mapping predicts earthquake surface-rupture locations

Abstract Earthquake surface-fault rupture location uncertainty is a key factor in fault displacement hazard analysis and informs hazard and risk mitigation strategies. Geologists often predict future rupture locations from fault mapping based on the geomorphology interpreted from remote-sensing data sets. However, surface processes can obscure fault location, fault traces may be mapped in error, and a future rupture may not break every fault trace. We assessed how well geomorphology-based fault mapping predicted surface ruptures for seven earthquakes: 1983 M 6.9 Borah Peak, 2004 M 6.0 Parkfield, 2010 M 7.2 El Mayor–Cucapah, 2011 M 6.7 Fukushima-Hamadori, 2014 M 6.0 South Napa, 2016 M 7.8 Kaikoura, and 2016 M 7 Kumamoto. We trained geoscience students to produce active fault maps using topography and imagery acquired before the earthquakes. A geologic professional completed a “control” map. Mappers used a new “geomorphic indicator ranking” approach to rank fault confidence based on geomorphologic landforms. We determined the accuracy of the mapped faults by comparing the fault maps to published rupture maps. We defined predicted ruptures as ruptures near a fault (50–200 m, depending on the fault confidence) that interacted with the landscape in a similar way to the fault. The mapped faults predicted between 12% to 68% of the principal rupture length for the studied earthquakes. The median separation distances between predicted ruptures and strong, distinct, or weak faults were 15–30 m. Our work highlights that mapping future fault ruptures is an underappreciated challenge of fault displacement hazard analysis—even for experts—with implications for risk management, engineering site assessments, and fault exclusion zones.

Agreement between cardiac output estimation with a wireless, wearable pulse decomposition analysis device and continuous thermodilution in post cardiac surgery intensive care unit patients.

Pulse Decomposition Analysis (PDA) uses integration of the systolic area of a distally transmitted aortic pulse as well as arterial stiffness estimates to compute cardiac output. We sought to assess agreement of cardiac output (CO) estimation between continuous pulmonary artery catheter (PAC) guided thermodilution (CO-CCO) and a wireless, wearable noninvasive device, (Vitalstream, Caretaker Medical, Charlottesville, VA), that utilizes the Pulse Decomposition Analysis (CO-PDA) method in postoperative cardiac surgery patients in the intensive care unit. CO-CCO measurements were compared with post processed CO-PDA measurements in prospectively enrolled adult cardiac surgical intensive care unit patients. Uncalibrated CO-PDA values were compared for accuracy with CO-CCO via a Bland-Altman analysis considering repeated measurements and a concordance analysis with a 10% exclusion zone. 259.7h of monitoring data from 41 patients matching 15,583 data points were analyzed. Mean CO-CCO was 5.55L/min, while mean values for the CO-PDA were 5.73L/min (mean of differences +- SD 0.79 ± 1.11L/min; limits of agreement - 1.43 to 3.01L/min), with a percentage error of 37.5%. CO-CCO correlation with CO-PDA was moderate (0.54) and concordance was 0.83. Compared with the CO-CCO Swan-Ganz, cardiac output measurements obtained using the CO-PDA were not interchangeable when using a 30% threshold. These preliminary results were within the 45% limits for minimally invasive devices, and pending further robust trials, the CO-PDA offers a noninvasive, wireless solution to complement and extend hemodynamic monitoring within and outside the ICU.

Transfer of 137Cs and 90Sr from soil-to-potato: Interpretation of the association from global fallout in Aomori to accidental release in Fukushima and Chornobyl

Ceasium-137 and 90Sr are major artificial radionuclides that have been released into the environment. Soil-to-plant transfer of radionuclides is an important route to food contamination. The radionuclide activity concentrations in crops must be quantitatively predicted for estimating the internal radiation doses from food ingestion. In this study, soil and potato samples were collected from three study sites contaminated with different sources of 137Cs and 90Sr: Aomori Prefecture (global fallout) and two accidental release areas (Fukushima Prefecture and the Chornobyl exclusion zone). The 137Cs activity concentrations in the soil and potato samples widely ranged from 1.0 to 250,000 and from 0.048 to 200,000 Bq kg−1 dry weight, respectively. The soil-to-potato transfer factor of 137Cs also ranged widely (0.0015–1.1) and decreased with increasing concentration of exchangeable K. Meanwhile, the activity concentrations of 90Sr in the soil and potato samples were 0.50–64,000 and 0.027–18,000 Bq kg−1 dry weight respectively, and the soil-to-potato transfer factor of 90Sr was 0.023–0.74, decreasing with increasing concentration of exchangeable Ca. The specific activity ratios of 137Cs/Cs and 90Sr/Sr in the exchangeable fraction were similar to those in potatoes, with a factor of 3 in the ±95 % confidence intervals over six orders of magnitude and a factor of 2 in the ±95 % confidence intervals over five orders of magnitude, respectively. According to the data, the accuracy of predicting the activity concentrations of 137Cs and 90Sr in potatoes can be improved by applying the specific activity ratios of 137Cs/Cs and 90Sr/Sr in the exchangeable fraction. This approach accounts for variable factors such as the effects of K and Ca fertilization and soil characteristics. It also emphasizes the benefit of determining the stable Cs and Sr concentrations in potatoes and other crops prior to possible future contamination.

Documenting Wordless Testimony

This article considers what it means to give plants a voice as witnesses to nuclear events. It examines two texts that attempt to represent the nonverbal testimony of irradiated plants through a hybrid approach of text and image: Sugihara Rieko’s Pilgrimage to the A-Bombed Trees (Hibakuju junrei, 2015) and Michael Marder and Anaïs Tondeur’s The Chernobyl Herbarium: Fragments of an Exploded Consciousness (2016). Published a year apart, both texts focus on the afterlife of nuclear catastrophes: the atomic bombing of Hiroshima in 1945 and the Chernobyl nuclear disaster in 1986. Sugihara’s book is an account of the hibakujumoku – trees that survived the atomic bombing of Hiroshima and continue to produce new growth to this day. Combining maps, photographs, interviews, and short essays, Pilgrimage to the A-Bombed Trees is intended as an immersive guidebook to the nearly 170 irradiated trees located within a two-kilometer radius around Hiroshima’s ground zero. It presents these trees as “living witnesses” whose subsequent flourishing has provided human survivors with a conceptual figuration of destructive plasticity. Marder and Tondeur’s The Chernobyl Herbarium formally resembles Sugihara’s Pilgrimage to the A-Bombed Trees, and likewise combines text (a combination of short theoretical essays and personal remembrances of the Chernobyl meltdown) and images (Tondeur’s photograms of plants grown in the exclusion zone) into a slim volume that calls for a rejection of language as the sole means of bearing witness. According to Marder, Tondeur’s photograms “bring out the testimony of the plant,” and yet the same procedure renders these plant witnesses “specimens,” full of radioactivity but devoid of life (something Sugihara’s book avoids through the medium of photography). The Chernobyl Herbarium is an attempt to capture, in fragmentary form, the limits of language in the conceptualization of plant life and in the proper conceptualization of the Chernobyl disaster. Ultimately, both texts are experiments to “think the unthinkable and represent the unrepresentable,” to borrow Marder’s words, through a deep, speculative engagement with the botanical realm. As wordless testimony is given voice through human–botanical intra-action, the plasticity of plant life (its capacity to adapt and change) is highlighted, and this allows us to locate moments of phytomorphism – the attribution of vegetal qualities to the human – in these two self-proclaimed “guidebooks.”

Wall-to-wall mapping of carbon loss within the Chornobyl Exclusion Zone after the 2020 catastrophic wildfire

Key messageWe propose a framework to derive the direct loss of aboveground carbon stocks after the 2020 wildfire in forests of the Chornobyl Exclusion Zone using optical and radar Sentinel satellite data. Carbon stocks were adequately predicted using stand-wise inventory data and local combustion factors where new field observations are impossible. Both the standalone Sentinel-1 backscatter delta (before and after fire) indicator and radar-based change model reliably predicted the associated carbon loss.ContextThe Chornobyl Exclusion Zone (CEZ) is a mosaic forest landscape undergoing dynamic natural disturbances. Local forests are mostly planted and have low ecosystem resilience against the negative impact of global climate and land use change. Carbon stock fluxes after wildfires in the area have not yet been quantified. However, the assessment of this and other ecosystem service flows is crucial in contaminated (both radioactively and by unexploded ordnance) landscapes of the CEZ.AimsThe aim of this study was to estimate carbon stock losses resulting from the catastrophic 2020 fires in the CEZ using satellite data, as field visitations or aerial surveys are impossible due to the ongoing war.MethodsThe aboveground carbon stock was predicted in a wall-to-wall manner using random forest modelling based on Sentinel data (both optical and synthetic aperture radar or SAR). We modelled the carbon stock loss using the change in Sentinel-1 backscatter before and after the fire events and local combustion factors.ResultsRandom forest models performed well (root-mean-square error (RMSE) of 22.6 MgC·ha−1 or 37% of the mean) to predict the pre-fire carbon stock. The modelled carbon loss was estimated to be 156.3 Gg C (9.8% of the carbon stock in burned forests or 1.5% at the CEZ level). The standalone SAR backscatter delta showed a higher RMSE than the modelled estimate but better systematic agreement (0.90 vs. 0.73). Scots pine (Pinus sylvestris L.)-dominated stands contributed the most to carbon stock loss, with 74% of forests burned in 2020.ConclusionThe change in SAR backscatter before and after a fire event can be used as a rough proxy indicator of aboveground carbon stock loss for timely carbon map updating. The model using SAR backscatter change and backscatter values prior to wildfire is able to reliably estimate carbon emissions when on-ground monitoring is impossible.

The surprising artificial radioactivity of glaciers

Glaciers accumulate significant amounts of fallout radionuclides from nuclear accidents and weapons testing – sometimes in the highest radioactive concentrations ever found outside of nuclear exclusion zones and test sites. Michael Allen digs into the depths of this unexpected issue and the associated risks as glaciers melt.

Developing virtual acoustic terrain for Urban Air Mobility trajectory planning

Urban Air Mobility (UAM) is a transformative concept that must operate harmoniously within the constraints imposed by societal impacts. Noise-aware flight trajectory planning can address UAM’s community noise concerns. However, the traditional trajectory optimization paradigm requires repetitive computations of a flight’s noise footprints in complex urban environments and is computationally expensive. In this work, we propose virtual acoustic terrain, a novel concept to enable an efficient trajectory optimization paradigm. By applying acoustic ray tracing and the principle of reciprocity in a complex urban environment, we convert different noise constraints into 3D exclusion zones which UAM operations should avoid to maintain limited noise impact. It combines with the physical urban terrain to define an acceptable fly zone for non-repetitive noise-aware trajectory optimization. This framework provides a new angle to future urban area airspace management and can also accommodate other forms of societal constraints.

CHORNOBYL AS TEXT IN THE WORKS BY MARKIIAN KAMYSH

Focused on the works “Oformliandiia”, “Scrap metal” (“Chormet”), and “Duckweed” (“Riaska”) by the modern Ukrainian writer Markiian Kamysh, the paper clarifies the peculiarities of the literary representation of the Chornobyl disaster at various levels of the fictional text, such as narratological, pictorial, thematic, and architectonic. While each work has its distinct structure, hero type, and set of issues, they all share a common thread of characters being involved in the exclusion zone.
 Kamysh takes a bold approach to developing the Chornobyl theme, emphasizing the dark aspects of life in the zone, the complex existential process of self-understanding, and long-term stays in the zone as a way to alleviate pain and talk effectively about trauma. Every visit to the exclusion zone is an important event in the inner life of the characters. The confessional nature of the narrative, reflections on the exclusion zone as a territory for self-understanding, self-therapy, relief from trauma, and therefore general spiritual healing, allow for “Oformliandia” to be considered an alternative existential post-traumatic text within the modern Ukrainian nuclear discourse.
 M. Kamysh emphasizes that staying in the exclusion territory opens opportunities for the hero of “Oformliandia” to deeper understand his belonging to the country, its history, traditions, and the emerging culture of ties with the zone. In the form of fiction, the writer illustrates the impact of the accident on the morals and values of the whole society and a particular person and offers his recipe for life after the Chornobyl accident and with the Chornobyl tragedy.
 The narrator-witness from “Oformliandia” is the author’s alter ego; this fact increases the degree of emotionality and truthfulness of the presented fictional material. Through his works “Oformliandiia”, “Chormet”, and “Riaska”, M. Kamysh marked a new direction for the understanding of the Chornobyl theme in Ukrainian literature in the 21st century.

Open-Source Intelligence for Detection of Radiological Events and Syndromes Following the Invasion of Ukraine in 2022: Observational Study.

On February 25, 2022, Russian forces took control of the Chernobyl power plant after continuous fighting within the Chernobyl exclusion zone. Continual events occurred in the month of March, which raised the risk of potential contamination of previously uncontaminated areas and the potential for impacts on human and environmental health. The disruption of war has caused interruptions to normal preventive activities, and radiation monitoring sensors have been nonfunctional. Open-source intelligence can be informative when formal reporting and data are unavailable. This paper aimed to demonstrate the value of open-source intelligence in Ukraine to identify signals of potential radiological events of health significance during the Ukrainian conflict. Data were collected from search terminology for radiobiological events and acute radiation syndrome detection between February 1 and March 20, 2022, using 2 open-source intelligence (OSINT) systems, EPIWATCH and Epitweetr. Both EPIWATCH and Epitweetr identified signals of potential radiobiological events throughout Ukraine, particularly on March 4 in Kyiv, Bucha, and Chernobyl. Open-source data can provide valuable intelligence and early warning about potential radiation hazards in conditions of war, where formal reporting and mitigation may be lacking, to enable timely emergency and public health responses.

Adult conspecific density affects Janzen-Connell patterns by modulating the recruitment exclusion zones.

Our model highlights the complex interconnection between NF intensity, stand density, and recruitment patterns explaining where and why the JC distribution occurs. Moreover, predicting the occurrence of JC in relation to stand density we clarify the relevance of this ecological phenomenon for future integration in plant community frameworks.

Financing marine conservation from restructured debt: a case study of the Seychelles

In the face of the threats posed to the oceans by a changing climate, the need for marine conservation programs grows rapidly. Scaling with this need demands sufficient funding to support ambitious conservation projects. This funding must be obtained from increasingly varied and innovative sources since private grants and government allocated funds has proved insufficient. Debt-for-nature swaps are a financial mechanism seeking to improve debt burden while setting up environmental programs. This method of debt restructuring has existed for decades, but is seeing a resurgence of use and interest in recent years. Here we present an exploratory case study of a Seychelles debt-for-nature swap which examines this financial mechanism’s ability to fund impactful conservation projects, particularly in marine Economic Exclusion Zones (EEZ) of Small Island Developing States (SIDS). The Seychelles finalized a conversion of their sovereign debt with Paris Club creditors and The Nature Conservancy as a broker in 2015 with the goal of creating a robust marine spatial plan (MSP). They received notable recognition for multiple novel aspects of this deal as well as the sheer scale of the marine space protected (400,000 sq. km), and thus serves as a robust case study to analyze if debt-for-nature swaps have evolved since its theoretical conception in 1984. Our research favors qualitative data by employing a case study approach which draws on semi-structured interviews with key informants, content analysis of online resources, and a literature review. This research suggests that while the model has yet to be cemented, the Seychelles case study is representative of a coming evolution in debt-for-nature swap practices. By examining the critical governance factors that were employed in the Seychelles, this research reveals key takeaways for future implementation and establishing national candidacy. The findings highlight debt status relative to the economy, political willpower, funding streams utilized, and the use of co-production practices. We show how the Seychelles case study demonstrates marked progress from the historical standard regarding sovereignty concerns and governance, but not concerning timescales and low converted sum. However, we note that this innovative debt-for-nature swap suggests that a new standard is possible and provides a new framework and set of best practices. In doing so, the Seychelles MSP can potentially lead the way for additional marine debt-for-nature swaps.

Chornobyl radiation spikes are not due to military vehicles disturbing soil

On 25th February 2022, increased gamma radiation dose rates were reported within the Chornobyl Exclusion Zone (CEZ). This coincided with Russian military vehicles entering the Ukrainian part of the CEZ from neighbouring Belarus. It was speculated that contaminated soil resuspension by vehicle movements or a leak from the Chornobyl Nuclear Power Plant complex may explain these spikes in radiation dose rates. The gamma dose rate monitoring network in the CEZ provides a crucial early warning system for releases of radioactivity to the environment and is part of the international safeguards for nuclear facilities. With the potential for further military action in the CEZ and concerns over nuclear safety, it is essential that such anomalous readings are investigated. We evaluate the hypotheses suggested to explain the apparent gamma dose rate increases, demonstrating that neither military vehicle-induced soil resuspension nor a leak from the Chornobyl Nuclear Power Plant are plausible. However, disruption of the Chornobyl base-station's reception of wireless signals from the gamma dose rate monitoring network in the CEZ may potentially explain the dose rate increases recorded.

The role of mouse-like rodents in the Chornobyl Exclusion Zone in the spread of blood-borne diseases

A feature of the conducted research is the study of a topical issue – the influence of external and internal factors on the prevalence of pathogens of blood-parasitic diseases. One of the most important external factors of influence is the exposure dose of γ-radiation, since the catching of mouse-like rodents was carried out at the landfills of the drained bed of the cooling reservoir of the Chernobyl Nuclear Power Plant. Therefore, the aim of the work was to study mouse-like rodents for the presence of causative agents of blood-parasitic diseases in natural populations under the influence of radioecological conditions of man-made transformation of the cooling reservoir of the Chernobyl Nuclear Power Plant. Mouse-like rodents of the genera Apodemus and Myodes were studied from three different locations of the cooling pond using parasitological methods. Radiological methods were used to determine the level of contamination of the specified three places where mouse-like rodents were caught using the γ- and β-dosimeter Pripyat RKS 20.3. Examination of blood smears of mouse-like rodents from three experimental polygons of the cooling reservoir of the Chernobyl Nuclear Power Plant revealed the presence of pathogens of blood-parasitic diseases in 93.8% of animals. In particular, such pathogens as Rickettsia spp., Ehrlichia spp., Bartonella spp., Babesia spp., Hepatozoon spp., Haemobartonella (Mycoplasma) spp., were identified. and spirochetes. The prevalence of zoonotic agents among mouse-like rodents of the Chernobyl Zone of radioactive contamination, as well as its dependence on the level of radioecological pollution of the respective landfills, was established. Therefore, according to the results of the conducted research, the presence of causative agents of blood-parasitic diseases among mouse-like rodents, which are both intermediate and their definitive hosts, including those from dangerous zoonoses, which, subject to the application of timely and complex preventive measures, will prevent human infection

Global variability and controls on the accumulation of fallout radionuclides in cryoconite

The accumulation of fallout radionuclides (FRNs) from nuclear weapons testing and nuclear accidents has been evaluated for over half a century in natural environments; however, until recently their distribution and abundance within glaciers have been poorly understood. Following a series of individual studies of FRNs, specifically 137Cs, 241Am and 210Pb, deposited on the surface of glaciers, we now understand that cryoconite, a material commonly found in the supraglacial environment, is a highly efficient accumulator of FRNs, both artificial and natural. However, the variability of FRN activity concentrations in cryoconite across the global cryosphere has never been assessed. This study thus aims to both synthesize current knowledge on FRNs in cryoconite and assess the controls on variability of activity concentrations. We present a global database of new and previously published data based on gamma spectrometry of cryoconite and proglacial sediments, and assess the extent to which a suite of environmental and physical factors can explain spatial variability in FRN activity concentrations in cryoconite. We show that FRNs are not only found in cryoconite on glaciers within close proximity to specific sources of radioactivity, but across the global cryosphere, and at activity concentrations up to three orders of magnitude higher than those found in soils and sediments in the surrounding environment. We also show that the organic content of cryoconite exerts a strong control on accumulation of FRNs, and that activity concentrations in cryoconite are some of the highest ever described in environmental matrices outside of nuclear exclusion zones, occasionally in excess of 10,000 Bq kg−1. These findings highlight a need for significant improvements in the understanding of the fate of legacy contaminants within glaciated catchments. Future interdisciplinary research is required on the mechanisms governing their accumulation, storage, and mobility, and their potential to create time-dependent impacts on downstream water quality and ecosystem sustainability.

”Iteratively nafionated water” in its solid phase at room temperature is in fact a mixture of lyophilized biological and non-biological contaminants

A novel solid phase of water that is stable at room temperature has been reported to be accessible from hydrophilic surfaces by repeated perturbation of interfacial layers. These intriguing studies seemed to highlight the importance of exclusion zones at surfaces and coherence domains in bulk water, leading to far-reaching speculations regarding the formation of life. Extensive physico-chemical analyses suggested novel properties of such presumably enriched surface water and its lyophilized aggregate, a solid form named Xerosydryle. Here, we carried out a detailed biological and biochemical analysis of iteratively perturbed surface water prepared from NafionTM sheets. We demonstrate that the observed phenomena most likely do not originate from a novel solid phase of water, but instead from the accumulation of microbiological and organic contaminants. Likewise, a sample of the lyophilized solid material − that was presumed to represent stable water aggregates − was found to largely consist of a conglomerate of bacteria, algae and synthetic materials which had inadvertently accumulated during the long period of sample processing in open containers of up to several weeks.

Multi-Omics Analysis of Vicia cracca Responses to Chronic Radiation Exposure in the Chernobyl Exclusion Zone.

Our understanding of the long-term consequences of chronic ionising radiation for living organisms remains scarce. Modern molecular biology techniques are helpful tools for researching pollutant effects on biota. To reveal the molecular phenotype of plants growing under chronic radiation exposure, we sampled Vicia cracca L. plants in the Chernobyl exclusion zone and areas with normal radiation backgrounds. We performed a detailed analysis of soil and gene expression patterns and conducted coordinated multi-omics analyses of plant samples, including transcriptomics, proteomics, and metabolomics. Plants growing under chronic radiation exposure showed complex and multidirectional biological effects, including significant alterations in the metabolism and gene expression patterns of irradiated plants. We revealed profound changes in carbon metabolism, nitrogen reallocation, and photosynthesis. These plants showed signs of DNA damage, redox imbalance, and stress responses. The upregulation of histones, chaperones, peroxidases, and secondary metabolism was noted.

Hydrological setting controls 137Cs and 90Sr concentrations in a headwater catchment in the Chornobyl Exclusion Zone

Concentration-discharge relationships are widely used to understand the hydrological processes controlling river water chemistry. This study investigates how hydrological processes affect radionuclide (137Cs and 90Sr) concentrations in surface water in headwater catchments within the Chornobyl Exclusion Zone (ChEZ) in Ukraine. In the flat wetland catchments, the depth of the saturated soil layer changes little throughout the year, but changes in the saturated soil surface area during snowmelt and immediately after rainfall affect water chemistry by changing the opportunities for contact between the surface water and the soil surface. On the other hand, in the slope catchments where there are few wetlands, the water chemistry of river water is governed by changes in the relative contributions of “shallow water” and “deep water” due to changes in the catchment water supply pathways feeding the rivers. In this study, no correlations were observed between dissolved or suspended 137Cs concentrations and either discharge rates or competitive cations, but the solid-liquid ratio of 137Cs was found to be significantly and negatively correlated with water temperature. However, 90Sr concentrations in surface water were found to be strongly related to the water pathways for each of the catchments. Moreover, contact between the surface water and the soil surface and changes in the relative contributions of shallow and deep waters to stream water were correlated with changes in 90Sr concentrations in surface water in wetland and slope catchments, respectively. The study concludes that 90Sr in rivers inside the ChEZ are strongly affected by the water pathways in headwater catchments. Additional studies will be necessary to clarify the details of sorption/desorption reactions.