Exclusion Zone Research Articles

Seasonal habitat selection and ranging of domestic cats (Felis catus) in rural and urban environments

Domestic cats (Felis catus) thrive at high densities alongside humans in urban and rural environments and are responsible for excessive wildlife predation worldwide. As urbanisation and farmland expands, and domestic cats inevitably reach previously unoccupied habitats, management plans will rely on understanding cat ranging behaviours. Cat movements and habitat selection may differ depending on their surroundings, and consequently, we sought to assess how male and female cat behaviours varied during different seasons in urban, suburban, and rural environments. In cities and farmland in the UK, the location of 56 owned cats (26 F:30 M) was recorded every 5 min for a total of 5237 h using GPS collars (454 ± 25 fixes over c. 4 days per individual). Urban and rural cats exhibited similar patterns of home ranges, maximum distances travelled from their owner’s house, and habitat selection, where they selected for built-up areas with good cover and avoided open spaces. Cats spent an average of 75% of their time outside their owners’ house or garden and therefore had great potential to encounter wild prey. Males in rural areas were almost twice as active than other males but all exhibited crepuscular activity patterns compared to cathemeral or diurnal females. In summer, cats had smaller home ranges and were more nocturnal, poentially concentrating their impacts around core areas during hotter months. Similarities in cat ranging behaviours across the urban–rural gradient suggest management plans can be equally applied in areas alongside cities as well as farmland. Buffer or exclusion zones of 750 m around protected areas would exclude 95% of cats, but specialised management, such as periodic confinement during specific active periods, could prove effective during vulnerable prey species’ breeding seasons. These findings improve our understanding of how cat ranging is affected by urbanisation under seasonal variation, and can be used to tailor management strategies as new species and populations are exposed to domestic cat predation.

Uptake from water and depuration of 137Cs and 90Sr by silver Prussian carp (Carassius gibelio)

To follow up field observations in the Chornobyl Exclusion Zone (ChEZ), a series of controlled model aquarium experiments were conducted to determine the uptake and depuration rates of 137Cs and 90Sr in silver Prussian carp (Carassius gibelio) in fresh water, varying in temperature from 5 to 27 °C, with daily feeding rates of 0–1.5 % fish weight day−1.In the present study, the 137Cs uptake rates in muscle tissues directly from water, 0.05–0.09 day−1 at temperatures of 5–27 °C, were significantly lower than previously reported for fish fed under natural conditions in contaminated lakes within the ChEZ. The rate of 90Sr uptake in bone tissues of silver Prussian carp varied from 0.055 day−1 at a water temperature of 5 °C and feeding rates ≤0.15 % fish weight day−1 to 1.5 ± 0.2 day−1 at a temperature of 27 ± 1 °С and at the highest tested feeding rate of 1.5 % day−1.The rate of decrease of 137Cs concentration in muscle tissues was kb = 0.0028 ± 0.0004 day−1 (T1/2 = 248 ± 35 days) at the lowest water temperature tested (5 °С). At water temperatures between 13 and 26 °С and a feeding rate of 0.15 % day−1, the rate increased to kb = 0.0071–0.0092 day−1 (T1/2 = 75–99 days).The rates of decrease of 90Sr activity concentration in bone tissues at water temperatures between 22 and 25 °С and a feeding rate of 0.5 % day−1 were kb=0.004–0.0014 day−1, and the associated biological half-life T1/2 ranged 50–160 days, respectively. The present work supported conclusions related to the main pathways of 137Cs and 90Sr uptake by silver Prussian carp, and demonstrated the usefulness of combining field and laboratory uptake and depuration experiments.

Intelligent control algorithm for dynamic positioning control system

Introduction: The dynamic positioning system resists the environmental forces such as wind, wave and current acting on the ship through the thruster, so that the ship can remain in the position required by the sea level as much as possible, and the operation is very convenient. But its current dynamic positioning ability can not meet people's needs.Methods: A Kalman filter based on untracked optimization was designed for dynamic positioning control system. Then the intelligent control algorithm is designed for the dynamic positioning top-level controller and thrust optimal distribution controller, which occupy an important position in the system, namely the adaptive weight variation particle swarm optimization algorithm and thrust optimal distribution algorithm.Results and Discussion: The average position error of three degrees of freedom after filter processing is 1.53 m. Compared with other mainstream controllers, the mean root error of controllers based on adaptive weight variation particle swarm optimization in environment A and B is 2.295 and 1.8 m, respectively. In environment C, the controller based on thrust optimization allocation algorithm can get the optimal solution when the full rotary thruster reaches the 7 s and the channel thruster reaches the 4 s. The thrust exclusion zone is crossed at 46 s in environment D. In the dynamic positioning capability curve of the system, the experimental hull can balance the different environmental loads at all angles. In summary, the intelligent control algorithm proposed in this paper can effectively improve the positioning ability of the dynamic positioning control system and meet the needs of people for ship navigation today.

К ВОПРОСАМ О ДУХОВНОЙ КУЛЬТУРЕ В СЕВЕРОКИТАЙСКОЙ ПРОВИНЦИИ МАНЧЖУРИИ В НАЧАЛЕ XX в.

The paper touches on the processes of formation of the Russian population on the border territory of North-East China in the exclusion zone of the Chinese Eastern Railway, and reveals aspects of spiritual culture. The main emphasis is on creating a religiously tolerant environment for settlers in Manchuria, which was a common policy for the entire population of the Far Eastern Region. Particular attention is paid to Baptists, which took an active position in the Far East. Research is based on a wide range of sources, archival funds, personal archives, newspaper articles, research and general scientific literature. Some aspects of the work of Protestant missionaries are reflected only in confessional sources (in the books of V.I. Franchuk, Y.F. Sablin, G.P. Vince). Historical-systemic, comparative chronological, statistical and other methods made it possible to examine part of the spiritual culture in the designated area. The article examines aspects of the spread of evangelical doctrine at the beginning of the 20th century. As a result of the active work of Baptist churches and missionaries, the Far Eastern Union of Baptists (FEBL) was formed, which extended its activities to the border areas. Foreign missions provided personnel and financial support to fellow believers, this contributed to the spread of the gospel message in the 10s of the 20th century, but by the end of the 20s and 30s the situation had changed, and cooperation with foreign organizations was unfavorable. According to the author, the activities of the Manchu Baptist communities were closely connected with the Russian Far East, on the territory of which the governing body of the DVSB was located (initially in Blagoveshchensk, and then in Khabarovsk). The adherents of Evangelical Christian Baptists were mostly Russian-speaking, but work was also carried out with the Chinese population. In turn, the Far Eastern Union of Baptists, through the territory of Northern China, had the opportunity to more quickly and without control receive various types of assistance from European and American churches: funds and spiritual literature for the development of missionary activities.

Uptake of technetium-99 by food crops under field conditions

Long-term field experiments have been carried out in the Chornobyl Exclusion zone to determine parameters describing technetium (99Tc) transfer into five food plants (Lettuce, Radish, Wheat, Bean, and Potato) from four types of soil, namely Podzoluvisol, Greyzem, Phaeozem, and Chernozem. Technetium was added to the soils under field conditions in a pertechnetate form. In the first two years, soil type had little effect on Tc uptake by plants. In the first and second years after contamination, the concentration ratios (CR), defined as 99Tc activity concentration in the crop (dry weight) divided by that in the soil (dry weight), for radish roots and lettuce leaves ranged from 60 to 210. For potato tubers, the CR was d 0.4–2.3, i.e., two orders of magnitude lower than for radish and lettuce, and for summer wheat grain it was lower at 0.6 ± 0.1. After 8–9 years, root uptake of 99Tc by wheat decreased by 3–7 fold (CR from 0.016 ± 0.005 to 0.12 ± 0.034) and only 13–22 % of the total 99Tc added remained in the upper 20 cm soil layers. The time taken for half of the added 99Tc to be removed from the 20-cm arable soil layer due to vertical migration and transfer to plants was short at c. 2–3 years.

Public acceptability of proposals to manage new takeaway food outlets near schools: cross-sectional analysis of the 2021 International Food Policy Study

ABSTRACT Global trends indicate that takeaway food is commonly accessible in neighbourhood food environments. Local governments in England can use spatial planning to manage the opening of new takeaway outlets in ‘takeaway management zones around schools’ (known sometimes as ‘exclusion zones’). We analysed data from the 2021 International Food Policy Study to investigate public acceptability of takeaway management zones around schools. Among adults living in Great Britain (n = 3323), 50.8% supported, 8.9% opposed, and 37.3% were neutral about the adoption of these zones. Almost three-quarters (70.4%) believed that these zones would help young people to eat better. Among 16-17 year olds (n = 354), 33.3% agreed that young people would consume takeaway food less often if there were fewer takeaways near schools. Using adjusted logistic regression, we identified multiple correlates of public support for and perceived effectiveness of takeaway management zones. Odds of support were strongest among adults reporting that there were currently too many takeaways in their neighbourhood food environment (odds ratio: 2.32; 95% confidence intervals: 1.61, 3.35). High levels of support alongside limited opposition indicate that proposals for takeaway management zones around schools would not receive substantial public disapproval. Policy makers should not, therefore, use limited public support to rationalise policy inertia.

Chromosomal disorders in Triticum aestivum subject to prolonged radionuclide pollution of soil from the Exclusion Zone of the Chornobyl Nuclear Power Plant

Due to the large number of nuclear power plants around the globe and the growing threat of radioactive accidents, a complex assessment of the biological consequences of the Chornobyl disaster is a relevant issue. Study of mutagenic activity of prolonged and chronic action of radionuclide pollution of soil in the Chornobyl Exclusion Zone long after the accident will allow us to identify the current level of threat they pose to the human genome and can reveal specific cytogenic markers of radionuclide environmental pollution. For this purpose, we germinated seeds of wheat of soft winter varieties Smuhlianka and Bohdana in soil samples from the Chornobyl Exclusion Zone 35 years after the disaster. Specific activity of Cs-137 and Sr-90 accounted for 4.5–28.2 kBq/kg. To determine the frequency and range of chromosomal aberrations, we used the anaphase-telophase method. In aberrant cells, we studied extracellular distribution of chromosomal disorders. The frequency of aberrant cells was 3.53–7.55 times above the spontaneous level. High mutagenic activity remained in the conditions of the lowest density of radionuclide pollution of soil. The range of chromosomal disorders mostly included paired fragments and bridges and containd chromosomal acentric rings, binding of chromosomes, myocardium, and lagging and leading chromosomes. Radionuclide pollution in the Chornobyl Exclusion Zone induced multiple cellular aberrations in the rhizome meristem, the share of which was the highest in case of low specific radionuclide pollution of soil, and exhibited a tendency towards decrease against the background of the highest density of radionuclides. Pollution of the environment with radionuclides as a result of disasters at nuclear energy objects poses a threat to the human genome and is a global problem, requiring state support to solve through systematic genetic monitoring in readionuclide-polluted territories and areas adjacent to them.

Changes in the number and outcome of takeaway food outlet planning applications in response to adoption of management zones around schools in England: A time series analysis

Physical exposure to takeaway food outlets (“takeaways”) is associated with poor diet and excess weight, which are leading causes of excess morbidity and mortality. At the end of 2017, 35 local authorities (LAs) in England had adopted takeaway management zones (or “exclusion zones”), which is an urban planning intervention designed to reduce physical exposure to takeaways around schools. In this nationwide, natural experimental study, we used interrupted time series analyses to estimate the impact of this intervention on changes in the total number of takeaway planning applications received by LAs and the percentage rejected, at both first decision and after any appeal, within management zones, per quarter of calendar year. Changes in these proximal process measures would precede downstream retail and health impacts. We observed an overall decrease in the number of applications received by intervention LAs at 12 months post-intervention (6.3 fewer, 95% CI -0.1, -12.5), and an increase in the percentage of applications that were rejected at first (additional 18.8%, 95% CI 3.7, 33.9) and final (additional 19.6%, 95% CI 4.7, 34.6) decision, the latter taking into account any appeal outcomes. This effect size for the number of planning applications was maintained at 24 months, although it was not statistically significant. We also identified three distinct sub-types of management zone regulations (full, town centre exempt, and time management zones). The changes observed in rejections were most prominent for full management zones (where the regulations are applied irrespective of overlap with town centres), where the percentage of applications rejected was increased by an additional 46.1% at 24 months. Our findings suggest that takeaway management zone policies may have the potential to curb the proliferation of new takeaways near schools and subsequently impact on population health.

A tuning-free moderate-scale burned area detection algorithm — a case study in chornobyl-contaminated region

ABSTRACT The predefined threshold is one of the main fundamental and methodological challenges underlying the uncertainty and delayed release of existing burned area (BA) products. To improve the accuracy and timeliness of BA mapping, a tuning-free moderate-scale BA detection algorithm (AT&RF algorithm) was proposed to carry out rapid BA mapping for the immediate post-fire assessment. A case study was carried out at the Chornobyl Exclusion Zone (ChEZ) to assess the performance of the algorithm. The evaluation results indicated that the algorithm successfully detected the BA in 2015 and 2020 in ChEZ, which outperforms the existing BA products by significantly decreasing the omission error by 18.6% and 15.1% and improving the Kappa coefficient and critical success index by 9.4% and 4.0% on average for MCD64A1 and FireCCI51 products, respectively. It is also possible to transfer the algorithm quickly to other regions without complicated adjustments and significantly improve the timeliness and accuracy compared to the existing global BA products for assessing environmental impact and scope.

INFLUENCE OF рН AND Ca<sup>2+</sup> IONS ON CHEMICAL COMPOSITION AND SORPTION OF <sup>137</sup>Cs BY CHERKASY BENTONITES (UKRAINE)

The safety of near-surface and deep radioactive waste storage facilities is based on a system of engineered and natural barriers. Significant degradation of the engineered barrier system composed of cemented waste matrixes covered by cement mixture, concrete compartments, and structures at the basement of the storage facility may cause radionuclide transfer from the facility to groundwater. Mixing of the cement and concrete with water leads to the formation of several various hydration products with subsequent leaching of Ca2+ ions and formation of hydroxyl ions (OH-), which affects the alkalinity of the water environment and the sorption properties of bentonite, as a component of the engineered barrier at the basement of near-surface facilities. The article presents the results of an experimental study of the influence of Ca2+ ion concentration and pH of the model solution (similar to groundwater composition at Vector Site in Chornobyl Exclusion Zone) on the elemental composition and sorption properties of natural (NB) and Na-modified (PBA-20) bentonites from the Cherkasy deposit concerning 137Cs at the Solid : Solution ratio of 1 : 100. Geochemical modeling suggests that addition of CaCl2 to test solution and resulting alkaline pH leads to precipitation of solids, mainly oxides, hydroxides, Fe oxyhydroxides (hematite, goethite, limonite), and Ca carbonates (calcite, aragonite, dolomite). Their role in Cs adsorption was evaluated. The concentration of structural elements (Si, Al) in bentonites practically does not change with Ca2+ ion concentration increase in the model solution, demonstrating the bentonite structure’s stability under these conditions. At the same time, an increase in the Ca concentration and a decrease in the Na concentration was found in the ion exchange complex of the bentonites if compared to the initial natural bentonite. This results in the transformation of Na-modified bentonite from Na, Ca-form to Ca, Na-form. The total sorption capacity of NB and PBA-20 bentonite concerning Cs+ ions at increased concentrations of Ca2+ ions and pH of the solution slightly decreases, though retaining high values of the degree of absorption (> 90 %). The total adsorption of Cs+ ions on NB and PBA-20 bentonites from model groundwater with the addition of CaCl2 from 16 to 960 mg/dm3 and increase of рН from 7.4 to 11.8 decreases with the increase in ionic strength, in particular, due to competition with Са2+ and Na+ ions. NB and PBA-20 bentonites of the Cherkasy deposit remain a reliable component of the liner at the repository basement owing to their main functional property – high absorption capacity for 137Cs, which is an important dose-forming radionuclide of short-lived low- and medium-level waste.

The Moderating Effect of Instant Runoff Voting

Instant runoff voting (IRV) has recently gained popularity as an alternative to plurality voting for political elections, with advocates claiming a range of advantages, including that it produces more moderate winners than plurality and could thus help address polarization. However, there is little theoretical backing for this claim, with existing evidence focused on case studies and simulations. In this work, we prove that IRV has a moderating effect relative to plurality voting in a precise sense, developed in a 1-dimensional Euclidean model of voter preferences. We develop a theory of exclusion zones, derived from properties of the voter distribution, which serve to show how moderate and extreme candidates interact during IRV vote tabulation. The theory allows us to prove that if voters are symmetrically distributed and not too concentrated at the extremes, IRV cannot elect an extreme candidate over a moderate. In contrast, we show plurality can and validate our results computationally. Our methods provide new frameworks for the analysis of voting systems, deriving exact winner distributions geometrically and establishing a connection between plurality voting and stick-breaking processes.

Abstract 7322: Polygenic adaptation and co-regulatory dynamics in Chernobyl wolves: Unveiling immune and oncogenic stress interactions with implications for human cancer resilience

Abstract Wolves living in the Chernobyl Exclusion Zone (CEZ) for at least six generations have adapted to high radiation exposure and are a unique model for studying genetic selection under extreme conditions of oncogenic stress. The objective of this study was to build off previously described signatures of adaptation in CEZ wolves, identify networks of genes associated with immune adaptation in CEZ wolves, and determine prognostic significance of these genes in human cancer datasets to provide insight into immune adaptation and cancer immunity. RNA from blood samples of wolves in CEZ, Belarus (BLR), and Yellowstone (YLS) were sequenced previously. Here we analyzed these data by mapping wolf transcripts to human orthologs. Radiation exposure (in CEZ and BLR) was quantified by intramuscular Cesium-137. Differential gene expression (DESeq2), network co-expression analysis (lmQCM), and gene set variation analysis (GSVA) assessed transcriptomic changes between populations. Germline single nucleotide polymorphism (SNP) for all three populations were inferred from RNA-seq data. Genes differentially regulated between populations, including gene modules showing signatures of polygenic selection through increased rates of genetic divergence (p< 0.05) in CEZ wolf populations, were deemed as outlier genes. Those n=3,180 CEZ outlier genes were then investigated in human cancer data from The Cancer Genome Atlas (TCGA) to determine prognostic impact in 10 tumor types highly conserved between human and canines. GSVA confirmed activation of neutrophil and macrophage signatures in CEZ wolves and suppression of several adaptive immune-related signatures (p < 0.05 vs BLR and YLS). A signature including immune checkpoint targets was also significantly differentially expressed in CEZ wolves. In TCGA, Cox PH analysis identified 23 genes over-expressed in CEZ wolves with significant positive prognostic impact in ≥ 2 human tumor types. PTPN6, previously identified to have positive prognostic value in bladder cancer1, was also prognostic in three additional tumor types. In human bladder cancer, genes identified as differentially regulated in CEZ wolves were disproportionately associated with improved survival. As predicted, gene signature variations in CEZ wolves reveal a distinct immune profile, likely shaped by prolonged radiation exposure. These findings, along with evidence of polygenic selection, suggest adaptation to multigenerational radiation exposure (an oncogenic stress). Notably, the enrichment of genes with positive prognosis in human cancer overexpressed in CEZ wolves present a valuable model to explore genetic underpinnings of cancer immunity and advance our understanding of cancer resilience in humans. Reference: 1Shen C. et al. The Analysis of PTPN6 for Bladder Cancer: An Exploratory Study Based on TCGA. Dis Markers. 2020. Citation Format: Christina Y. Yu, Cara N. Love, Merzu Belete, Hareesh Chandrupatla, Lauren K. Brady, Sriram Sridhar, Shane C. Campbell-Staton. Polygenic adaptation and co-regulatory dynamics in Chernobyl wolves: Unveiling immune and oncogenic stress interactions with implications for human cancer resilience [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 7322.

Ungulate herbivores promote contrasting modifications of soil properties and organic carbon stabilization in a grazed grassland versus rewilded woodland environment

Large vertebrate herbivores are ecosystem engineers, provoking cascading effects on soil properties through modifications of plant communities as well as direct modulation of organic matter incorporated into soils. However, most studies have been carried out with domestic ungulates in grasslands, and the effects which predominate in grasslands may not be generalizable to other ecosystems. It is not well understood how trophic rewilding with wild ungulates may affect soil processes, especially in non-grassland systems albeit rewilding of natural areas is a process associated with larger trends of land abandonment, shrub encroachment or land use change. In this study we investigated ungulate herbivore exclusion in two geographically and pedo-climatically equivalent but ecologically distinct contexts: a Mediterranean grassland with domesticated herbivores, and a reforested area populated by a community of wild ungulates. In each context, long-term herbivore exclusion zones were used to understand the effect of herbivores on soil properties and particularly on soil organic carbon (SOC). Samples from the topsoil (0–5 cm) were taken inside and outside the exclosures distributed between the two contexts, and ecosystem-level effects and responses to grazing were investigated. We found that soil properties’ response to grazing depended on the ecological context in which the grazing occurred. SOC contents in the grassland increased by up to 37% with grazing, while grazing reduced SOC concentrations by 23% in the rewilded area. In both cases, grazing was also associated with significant alterations of soil microbial biomass, the microbial metabolic quotient (qCO2), and aggregate stability, but the effects were opposite: On one hand, domesticated herbivores in the grassland positively influenced carbon stabilization which was strongly associated with soil microbial processes and characteristics, and additionally the proportion of stable macroaggregates was increased. In contrast, wild ungulate herbivory in the forest ecosystem reduced SOC concentrations, accompanied by decreased microbial biomass and increased microbial maintenance costs, and stable macroaggregates were reduced. The results indicate the importance of ecological context in determining the effect of herbivores on soil properties and processes and suggest that the directionality of effects on SOC may change owing to the predominance of different mechanisms of SOC stabilization. Consequently, this study provides evidence that the management of ungulates in rangelands or forests can be associated with different outcomes as related to crucial soil properties such as SOC contents and aggregation.

Active Gait System for Real-Time Surveillance Against Cyber-Physical Attacks

Cyberterrorism, espionage, and warfare are serious threats to national security. These attacks can harm people or destroy critical infrastructures like the data centres, computer networks, and systems. Surveillance systems currently used in monitoring critical infrastructures, national key points, and military exclusion zones (MEZ) are ineffective in detecting unauthorised intrusions. These issues compromise the stability of the countries, and the safety of the citizens and result in the loss of important assets. This experimental research study developed a Cyber Physical Security (CPS) defense gait-recognition monitoring system. Autonomous Machine Learning (ML) technology was employed to enhance the precision and reliability of the system against CPA, in tracking access, managing security clearances, and triggering alerts in the event of unauthorized entries to restricted areas.

Changes in the number of new takeaway food outlets associated with adoption of management zones around schools: A natural experimental evaluation in England

By the end of 2017, 35 local authorities (LAs) across England had adopted takeaway management zones (or “exclusion zones”) around schools as a means to curb proliferation of new takeaways. In this nationwide, natural experimental study, we evaluated the impact of management zones on takeaway retail, including unintended displacement of takeaways to areas immediately beyond management zones, and impacts on chain fast-food outlets. We used uncontrolled interrupted time series analyses to estimate changes from up to six years pre- and post-adoption of takeaway management zones around schools. We evaluated three outcomes: mean number of new takeaways within management zones (and by three identified sub-types: full management, town centre exempt and time management zones); mean number on the periphery of management zones (i.e. within an additional 100 m of the edge of zones); and presence of new chain fast-food outlets within management zones. For 26 LAs, we observed an overall decrease in the number of new takeaways opening within management zones. Six years post-intervention, we observed 0.83 (95% CI -0.30, −1.03) fewer new outlets opening per LA than would have been expected in absence of the intervention, equivalent to an 81.0% (95% CI -29.1, −100) reduction in the number of new outlets. Cumulatively, 12 (54%) fewer new takeaways opened than would have been expected over the six-year post-intervention period. When stratified by policy type, effects were most prominent for full management zones and town centre exempt zones. Estimates of intervention effects on numbers of new takeaways on the periphery of management zones, and on the presence of new chain fast-food outlets within management zones, did not meet statistical significance. Our findings suggest that management zone policies were able to demonstrably curb the proliferation of new takeaways. Modelling studies are required to measure the possible population health impacts associated with this change.

Gradient-based wind farm layout optimization with inclusion and exclusion zones

Abstract. Wind farm layout optimization is usually subjected to boundary constraints of irregular shapes. The analytical expressions of these shapes are rarely available, and, consequently, it can be challenging to include them in the mathematical formulation of the problem. This paper presents a new methodology to integrate multiple disconnected and irregular domain boundaries in wind farm layout optimization problems. The method relies on the analytical gradients of the distances between wind turbine locations and boundaries, which are represented by polygons. This parameterized representation of boundary locations allows for a continuous optimization formulation. A limitation of the method, if combined with gradient-based solvers, is that wind turbines are placed within the nearest polygons when the optimization is started in order to satisfy the boundary constraints; thus the allocation of wind turbines per polygon is highly dependent on the initial guess. To overcome this and improve the quality of the solutions, two independent strategies are proposed. A case study is presented to demonstrate the applicability of the method and the proposed strategies. In this study, a wind farm layout is optimized in order to maximize the annual energy production (AEP) in a non-uniform wind resource site. The problem is constrained by the minimum distance between wind turbines and five irregular polygon boundaries, defined as inclusion zones. Initial guesses are used to instantiate the optimization problem, which is solved following three independent approaches: (1) a baseline approach that uses a gradient-based solver; (2) approach 1 combined with the relaxation of the boundaries, which allows for a better design space exploration; and (3) the application of a heuristic algorithm, “smart-start”, prior to the gradient-based optimization, improving the allocation of wind turbines within the inclusion polygons based on the potential wind resource and the available area. The results show that the relaxation of boundaries combined with a gradient-based solver achieves on average +10.2 % of AEP over the baseline, whilst the smart-start algorithm, combined with a gradient-based solver, finds on average +20.5 % of AEP with respect to the baseline and +9.4 % of AEP with respect to the relaxation strategy.

Environmental radiation exposure at Chornobyl has not systematically affected the genomes or chemical mutagen tolerance phenotypes of local worms

The 1986 disaster at the Chornobyl Nuclear Power Plant transformed the surrounding region into the most radioactive landscape known on the planet. Whether or not this sudden environmental shift selected for species, or even individuals within a species, that are naturally more resistant to mutagen exposure remains an open question. In this study, we collected, cultured, and cryopreserved 298 wild nematode isolates from areas varying in radioactivity within the Chornobyl Exclusion Zone. We sequenced and assembled genomes de novo for 20 Oscheius tipulae strains, analyzed their genomes for evidence of recent mutation acquisition in the field, and observed no evidence of an association between mutation and radioactivity at the sites of collection. Multigenerational exposure of each of these strains to several chemical mutagens in the lab revealed that strains vary heritably in tolerance to each mutagen, but mutagen tolerance cannot be predicted based on the radiation levels at collection sites, and Chornobyl isolates were not systematically more resistant than strains from undisturbed habitats. In sum, the absence of mutational signatures does not reflect unique capacity for tolerating DNA damage.

Sulfobetaine-Siloxanes: A Class of Self-Destructive Surfactants.

The hydrolytic susceptibility of sulfobetaine-siloxane surfactants is investigated by comparison of a homologous series in this subclass of surfactants (R-(CH2)3N+(Me)2(CH2)3SO3-; R = (Me3SiO)3Si-, (Me3SiO)2Si(Me)-, (Me2SiO)3-Si(Me)-) with an analogue series of oxyethylene-siloxane surfactants (R-(CH2)3(OCH2CH2)10.2OH; R = (Me3SiO)3Si-, (Me3SiO)2Si(Me)-, (Me2SiO)3-Si(Me)-). Nuclear magnetic resonance (NMR) monitoring of these surfactants in an aqueous solution shows that the presence of the sulfobetaine head structure greatly enhances the hydrolysis rate of the siloxane tail as compared with oxyethylene-siloxane analogue control experiments. This sulfobetaine effect is confirmed by adding a model compound, (Me)3N+(CH2)3SO3-, to the oxyethylene-siloxane surfactants and observing the large hydrolysis enhancement. Measurements of pH indicate the sulfobetaine presence greatly enhances acidity, but rigorous analysis could discover no source of acid other than the presence of the sulfobetaine structure. Titration measurements confirmed the presence of a tightly bound hydration layer of 4-7 water molecules per sulfobetaine group. It is speculated that the source of acidity may originate from an aqueous exclusion zone nucleated by the hydrated sulfobetaine at the interface of a sulfobetaine-siloxane surfactant bilayer aggregate. Hydrolysis prevention is investigated by addition of a pH 7 phosphate buffer, of an alkyl polyglycoside cosurfactant, and of a combination of both, with a finding of very significant but not complete suppression of the hydrolysis.

War drives forest fire risks and highlights the need for more ecologically-sound forest management in post-war Ukraine.

Since 24 February 2022, Ukraine has experienced full-scale military aggression initiated by the Russian Federation. The war has had a major negative impact on vegetation cover of war-affected regions. We explored interactions between pre-war forest management and the impacts of military activities in three of the most forested Ukrainian areas of interest (AOI), affected by the war. These were forests lying between Kharkiv and Luhansk cities (AOI 'East'), forests along the Dnipro River delta (AOI 'Kherson'), and those of the Chornobyl Exclusion Zone (AOI CEZ). We used Sentinel satellite imagery to create damaged forest cover masks for the year 2022. We mapped forests with elevated fire hazard, which was defined as a degree of exposure to the fire-supporting land use (mostly an agricultural land, a common source of ignitions in Ukraine). We evaluated the forest disturbance rate in 2022, as compared to pre-war rates. We documented significant increases in non-stand replacing disturbances (low severity fires and non-fire disturbances) for all three of the AOIs. Damaged forest cover varied among the AOIs (24,180 ± 4,715ha, or 9.3% ± 1.8% in the 'East' AOI; 7,293 ± 1,925ha, or 15.7% ± 4.1% in the 'Kherson' AOI; 7,116 ± 1,274ha, or 5.0% ± 0.9% in the CEZ AOI). Among the forests damaged in 2022, the 'Kherson' AOI will likely have the highest proportion of an area with elevated fire hazard in the coming decades, as compared to other regions (89% vs. 70% in the 'East' and CEZ AOIs respectively). Future fire risks and extensive war-related disturbance of forest cover call for forest management to develop strategies explicitly addressing these factors.

Nuclear accident sites and tourism: a comparative analysis of the Chernobyl and Fukushima exclusion zones

ABSTRACT This article aims to explore similarities and differences in the relationship between nuclear accident sites and tourism through a comparative study of two culturally different exclusion zones, namely Chernobyl (Ukraine) and Fukushima (Japan). Although the causes of the nuclear catastrophe were different, today it is possible to confirm some similarities in the transition and recovery process between these contaminated and abandoned sites. A range of grassroots initiatives lead to movements that try to look at nuclear accident sites from a new perspective. These activities include the ones that are planned and conducted to develop the sites as tourist destinations. In addition, in the context of war in Ukraine, the article shows, through the Stone’s darkest-lightest indicator, how the narration of the places of cultural dissonances changes with the changing geopolitical reality. In order to achieve the aim of this paper, a comparative analysis was conducted referring to qualitative data collected in the Chernobyl Exclusion Zone and secondary data on both Chernobyl and Fukushima exclusion zones. The following interviews with tourists, participatory observation and collected online sources of these two post-catastrophe sites have first and foremost provided a better understanding of the cultural dissonances emerging in these spaces.