Efficient lithium extraction via electrochemical adsorption holds great promise for resource utilization and energy storage. However, the mechanism of how temperature influences the electrochemical adsorption process, particularly the thermodynamics and dynamics in the liquid, liquid–solid interface, and solid remains unclear. To address this challenge, we explored the impact of temperature (273.15 to 328.15 K) using Li1-xMn2O4 as an adsorbent. For the liquid phase, the conductivity increased from 0.89 to 0.95 S/m following lnΛ = -0.11(1000/T) + 0.27 in thermodynamics; the solution viscosity decreased from 1.71 × 10-3 to 5.15 × 10-4 Pa·s conforming lnη = 1.96(1000/T) − 13.54 in dynamics. For the liquid–solid interface, the charge transfer resistance (Rct) decreased from 42.03 to 25.17 ohm obeying ln(1/Rct) = -0.82(1000/T) − 0.69 in thermodynamics; the exchange current (i0) showed a positive correlation with temperature in fitting Logi0 = -0.50(1000/T) − 1.40 in dynamics. For the solid phase, the increase in reduction peak voltage (ERP) and the decrease in Gibbs free energy (G), entropy (S), and enthalpy (H) all indicate that the increase of temperature promotes lithium ions to inserted into Li1-xMn2O4 in thermodynamics; the increased diffusion coefficient (DLi+(S)) and higher reduction peak current (IRP) suggest that higher temperature strengthens lithium ions diffusion and insertion in dynamics. In the temperature range, the adsorption capacity gradually increased from 1.15 to 7.37 mg/g, the initial current enhanced from −3.40 × 10-4 to −1.85 × 10-3 A, the terminal current raised from −1.59 × 10-4 to −7.40 × 10-4 A, and the current efficiency improved from 31.28 % to 66.11 % in the temperature range. This work provides a deeper understanding of the impact of temperature on the electrochemical lithium adsorption process from thermodynamics and dynamics. This work also offers crucial guidance for the upgrading of electrochemical lithium adsorption technology and can be applied to other fields such as energy storage, catalysis, and synthesis, providing a valuable reference for optimizing reaction parameter design in thermodynamics and dynamics.