Excessive Use Research Articles

A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder.

Alcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to determine the methylation levels of two crucial genes (GHRL and GHSR) in ghrelin signaling and further investigate the association between methylation ratios and plasma ghrelin levels. Individuals diagnosed with (n = 71) and without (n = 82) AUD were recruited in this study. DNA methylation levels were measured through methylation-sensitive high-resolution melting (MS-HRM). Acylated ghrelin levels were detected by ELISA. The GHRL rs696217 polymorphism was analyzed by the standard PCR-RFLP method. GHRL was significantly hypermethylated (P < 0.0022) in AUD between 25 and 50% methylation than in control subjects but no significant changes of GHSR methylation were observed. Moreover, GHRL showed significant positive correlation of methylation ratio between 25 and 50% with age. A significant positive correlation between GHSR methylation and ghrelin levels in the AUD group was determined (P = 0.037). The level of GHRL methylation and the ghrelin levels showed a significant association in the control subjects (P = 0.042). GHSR and GHRL methylation levels did not change significantly between control and AUD groups. However, GHRL and GHSR methylations seemed to have associations with plasma ghrelin levels in two groups. This is the first study investigating the DNA methylation of GHRL and GHSR genes in AUD.

N2O emission factors in bubbling fluidized bed incineration of municipal sewage sludge: The China case

The N2O emissions resulting from sludge incineration are estimated using the default values published by the Intergovernmental Panel on Climate Change (IPCC), which may differ significantly from the actual emissions. In this investigation, N2O emissions from four sludge incineration lines in two plants were monitored for varying durations. The variation in N2O emission factors (EFs) between incineration lines of the same plant was much smaller than the difference between different plants. Data on N2O EFs obtained from brief monitoring may contain variabilities of up to 30%. N2O EFs were more sensitive to temperature changes at low temperatures, necessitating extended monitoring periods to improve the reliability of N2O monitoring outcomes in cases of low furnace temperatures. Excessive use of the SNCR system to reduce NOx emissions resulted in concentrations of N2O and NH3 in the exhaust gases exceeding NOx levels. In the case of furnace temperature control and advanced reburning technology, it is advisable to utilize actual monitoring data or the smaller default values provided by the IPCC in China. Otherwise, the estimated N2O emissions may exceed the actual emissions.

Remediation of arsenic and fluoride from groundwater: a critical review on bioadsorption, mechanism, future application, and challenges for water purification.

In the past few decades, the excessive and inadequate use of technological advances has led to groundwater contamination, mainly caused by organic and inorganic pollutants, which are highly harmful to human health, agriculture, water bodies, and aquaculture. Among all toxic pollutants, As and F- play a significant role in groundwater contamination due to their excellent reactivity with other elements. To mitigate the prevalence of arsenic and fluoride within the water system, the use of biochar gives an attractive strategy for removing them mainly because of the substantial surface area, pore size, pH, aromatic structure, and functional groups inherent in biochar, which are primarily dependent upon its raw material and pyrolysis temperature. Researcher develops different methods like physiochemical and electrochemical for treating arsenic and fluoride contamination. Among all removal methods, bioadsorption using agricultural waste residues shows effective/feasible removal of As and F- due to its low cost, ecofriendly nature, readily available, and efficient reuse compared with several other harmful synthetic materials that demand costly design specifications. This study discusses current developments in bioadsorption methods for As and F- that use agricultural-based biomaterials and describes the prevailing state of arsenic and fluoride removal strategies that use biomaterials precisely.

Fertilizer Optimization through Machine Learning-driven Models: An Empirical Investigation on Smart Farming of Amaranth

Amaranth is a highly nutritious leafy vegetable cum pseudo-cereal crop known for its adaptability to various climatic conditions, making it a promising crop for addressing the food security and nutritional needs of a growing population. To enhance its quality and boost yields, farmers mainly depend on synthetic fertilizers. However, the excessive use of inorganic fertilizers to maximize crop yields poses significant ecological risks. This study aimed to investigate the impact of excessive inorganic fertilizer on the growth, yield, and physiological attributes of Amaranthus with the aid of advanced machine learning paradigm. An experimental pot trial was conducted using different NPK fertilizer dosage regimes, and agronomic parameters such as moisture level, crop yield, plant height, leaf length, leaf width etc. were measured and analyzed using statistical methods. The results demonstrated that the application of excessive inorganic fertilizer initially promoted plant growth but surpassed optimal levels resulting in negative effects, including stunted growth and reduced vigor. By identifying the Amaranthus's productivity and adaptability in different chemically treated soil conditions and automatically phenotyping its traits using image-based machine learning models, this study aims to determine the overuse of synthetic fertilizers. A comparative evaluation of different learning algorithms was carried out and the experimental result proves that SVM classifier could be a more appropriate learning algorithm for the proposed system with 80% accuracy. These findings highlight the importance of adopting sustainable fertilizer practices for the cultivation of Amaranthus and emphasize the need for ecological balance in crop production systems.

Epidemiological Profile of Cases of Hair Cosmetic Poisoning and Investigation of Risk Factors in Morocco (1981-2011)

Intoxications from cosmetic products are common incidents resulting from inappropriate or excessive use, whether accidental or intentional, through ingestion, inhalation, or skin absorption of substances present in cosmetic products. The aim of this retrospective study is to establish the epidemiological profile of hair cosmetic poisoning cases reported to the Moroccan Poison Control and Pharmacovigilance Center (CAPM) between 1981 and 2011. The results indicate a predominance of females (74.12%). However, 54.11% are adults, 20% are infants + toddlers, and 24.70% are adolescent children. Furthermore, 66.67% of them suffered from hair dye and coloring intoxication. On the other hand, a majority of 41% showed a slight risk or no risk at all. Another notable observation is that 39% of the victims have shown a moderate risk. Furthermore, it is worth mentioning that the fatality rate is 1.1%. Furthermore, there is a need to raise awareness among the public and the media about the dangers of using certain cosmetic products.

Excess Use of Surgical Supplies in Minimally Invasive Benign Gynecology Surgery: An Observational Study.

Single use materials and equipment are regularly opened by the surgical team during procedures but left unused, potentially resulting in superfluous costs and excess environmental waste. The aim of this study is to estimate the excess use of surgical supplies in minimally invasive benign gynecological surgeries. This is a prospective observational study conducted at a university affiliated single tertiary medical center. Designated study personnel were assigned to observe surgical procedures performed during July to September 2022. Surgical teams were observed while performing surgeries for benign indications. The teams were not informed of the purpose of the observation to avoid potential bias. Disposable materials and equipment opened during the procedure were documented. Excess supplies were defined as those opened but left unused before being discarded. Costs per item of the excess supplies were estimated based on material and equipment costs provided by the hospital. 99 surgeries were observed, including laparoscopic (32%), robotic (39%), hysteroscopic (14%), vaginal (11%) and laparotomy procedures (3%). Excess use of surgical supplies was documented in all but one procedure. The total cost across all surgeries reached $6,357. Contained tissue extraction bag was the most expensive item not used (Applied Medical, $390 per unit), in 4 procedures, contributing 25.54% to the total cost. Raytec was the most common surgical waste with a total of n=583 opened but unused (average n=5.95 per surgery). A significant difference was found in the rate of excess supplies across the surgical approaches, with robotic surgery contributed 52.19% of the total cost (p=0.01). Excess use of disposable materials and equipment is common in minimally invasive benign gynecological surgeries and contributes to the superfluous costs and the excess environmental waste. It is predominantly attributed to opening of inexpensive materials that are left unused during the procedure. Increased awareness of costs and generated waste may reduce excess use of surgical supplies and should be further explored in future research.

Analysis of Soil Samples for its Physicochemical Parameters of Soil in Bawal Block of Haryana

Without a question, the oldest occupation on Earth is agriculture. The soil was virgin in the early stages of evolution, and farming was done very differently than it is now. Growing more food in a less amount of space has made it necessary to plant more crops more frequently and to use omnicides and fertilizers frequently. Excessive use of fertilizers and omnicides negatively impacts soil characteristics, air and water pollution levels. The study of agricultural science or soil science has piqued people's curiosity. An attempt has been made to address the aforementioned issues in this work. The soil in the area being studied has been physicochemical characterized. Key Words: Water Holding Capacity, High-performance Liquid Chromatography, Nutritive index, Fertility index

The effects of mobile phone dependence on athletic performance and its mechanisms

Mobile phone dependence (also known as internet dependence, MPD), defined as a problematic behavior characterized by excessive use or intermittent craving to use a mobile phone, results in various social, behavioral, and affective problems in daily life. In sports, MPD is directly related to the physical and mental health and sports performance of athletes. The individual and environmental factors, neurobiological mechanisms and theoretical models of MPD affecting athletic performance were analyzed by reviewing previous studies, aiming to construct effective training and development protocols to prevent and control the occurrence of MPD in athletes. At present, athletic performance can be affected by MPD through individual factors and environmental factors. The neurobiological mechanisms between the two are based on the brain reward system and microwave radiation from mobile phones, with athletic performance being restricted by alterations in the corresponding brain regions. Relevant theoretical models mainly include the social cognitive model of self-regulation and the integrative model of self-control, which explain the interrelationship between MPD and athletic performance from the perspectives of athletes’ self-regulation and self-control, respectively. As an emerging phenomenon, the influence pathways and mechanisms by which MPD affects athletic performance need to be further investigated. A longitudinal perspective should be adopted to trace the dynamic impact relationship between the two, and developing relevant theoretical frameworks from an interdisciplinary research perspective should be valuable for providing theoretical support for coaches and sports administrators to formulate scientific training protocols and thus improve the mental health of athletes.

Use of Non-Chlorine Sanitizers in Improving Quality and Safety of Marketed Fresh Salad Vegetables

The safety of vegetable food is compromised by various factors, including the inefficient or excessive use of sanitizers. Instances of individuals falling ill after consuming raw vegetables have been reported, with outbreaks of diseases caused by pathogens on fresh vegetables becoming increasingly prevalent globally, attracting significant media coverage and impacting the economic viability of vegetable cultivation. Measures to enhance food safety in postharvest horticultural produce involve controlling microbial proliferation and minimizing cross-contamination. Sanitizers were utilized in the food safety arsenal for a variety of purposes, including pathogen elimination and microbe reduction, hand, tool, and vegetable contact surface cleaning, and produce shelf-life extension. Choosing an appropriate sanitizer for all vegetables is difficult due to a lack of knowledge on which sanitizers are ideal for the many types of vegetables grown on farms under different environmental circumstances. Although chlorine-based sanitizers, such as sodium or calcium hypochlorite, have been widely used for the past 50 years, recent research has revealed that chlorine reacts with an organic compound in fresh vegetables to produce trihalomethane, a carcinogen precursor, and as a result, many countries have prohibited the use of chlorine in all foods. As a result, horticulture research groups worldwide are exploring non-chlorine, ecologically friendly sanitizers for the vegetable industry. They also want to understand more about the present procedures in the vegetable business for employing alternative sanitizers, as well as the efficacy and potential dangers to the food safety of fresh salad vegetables. This review paper presents detailed information on non-chlorine sanitizers, such as their efficacy, benefits, drawbacks, regulatory requirements, and the need for additional research to lower the risk of marketed salad vegetable food safety.

The Clinical Sequelae of the COVID-19 Pandemic: Loneliness, Depression, Excessive Alcohol Use, Social Media Addiction, and Risk for Suicide Ideation

Background Depression, loneliness, and alcohol use disorder are associated with suicide ideation. The ongoing COVID-19 pandemic has challenged our social structures with social distancing and isolation policies implemented worldwide, severely restricting social interactions. Studies regarding the effects of the pandemic are starting to shed light on the harmful psychological effects of these policies. Aims This study aims to identify whether the increase in suicidal ideation among college students (mostly young adults) during the pandemic was due to the known risk factors of loneliness, depression, alcohol use disorder, social media addiction, and other background variables. Method Nine hundred and eleven college students completed self-report questionnaires assessing suicidal risk, depressive symptoms, loneliness, excessive alcohol use, and social media use. Results During the pandemic suicidal ideation was associated with loneliness (χ2 = 54.65, p < 0.001), depressive symptoms (χ2 = 110.82, p < 0.001), alcohol use disorder (χ2 = 10.02, P < 0.01) and social media addiction (χ2 = 13.73, P < 0.001). Being single [OR = 2.55; p < 0.01], and self-identifying as a non-heterosexual [OR = 2.55; p < 0.01] were found to constitute additional risk factors. Limitations The structural nature of quantitative self-report scales does not offer the flexibility of gaining a deeper understanding of causes, specific to particular circumstances that may lead participants to ideate on suicide, even briefly. Conclusions Social distancing and isolation policies during the COVID-19 pandemic constitute an additional factor in the risk for suicide ideation.

Effect of Intercropping with Cowpea and Maize with Organic Manure Application on the Physiological Parameters

Intercropping involves cultivating two or more crops simultaneously in the same field, optimizing the use of land, water, light, and nutrients for higher yields compared to sole cropping. Organic manures, such as farmyard manure, poultry manure, oilseed cake, vermicompost, and bio-slurry, play a vital role in enhancing soil health by providing essential nutrients and supporting microbial populations. In contrast, excessive use of inorganic fertilizers can lead to environmental degradation. Present study explores the synergistic effects of intercropping and organic manure application on the physiological parameters of maize (Zea mays) and cowpea (Vigna unguiculata). Agricultural practices like intercropping optimize resource utilization and enhance overall productivity, especially in regions with limited water resources like Mizoram, India. These practices exhibit positive responds when combined with organic manure on soil health and yield attributes on crops. The experiment was laid out in randomized block design (RBD) with ten treatments replicated thrice. The result indicate that plants treated with Maize + Cowpea (2:1) + 50% of RDN (Recommended doses of Nitrogen) through Farm Yard Manure + 50% of RDN through Poultry Manure + Lime (200 kg ha-1), resulted in significantly maximum physiological parameters of the crops.

Synthesis of Schiff‐Base Allied Silanes for the Photophysical Detection of Cr(III) Ion And In‐Silico Staphylococcus Aureus Inhibiting Activity

AbstractChromium is known to play an important role in various metabolic pathways; however, its excessive use can result in negative impact on mankind, this necessitates the development of sensors for Cr(III) ion. The current study has incorporated the synthesis of ether derived Schiff base allied silanes 4 a–4 d, which have been characterized using 1H NMR, 13C NMR and mass spectrometry. The spectroscopic technique of UV‐visible has been utilized to study the sensing behaviour of probe 4 a, resulting in a good sensing ability towards Cr(III) ion, yielding a detection limit of 4.2×10−7 M and an association constant value of 2.25×106 M−1. In order to study the stoichiometric binding between probe and metal ion, Job's plot method has been employed which shows 1 : 1 binding ratio. The mechanism of binding has been studied via 1H NMR, UV‐visible, FT‐IR spectroscopy and mass spectrometry. The binding has been further supported by DFT. The real sample analysis has been performed to validate the utility of the synthesized sensor in practical applications. The theoretical tool of molecular docking has been considered to study the protein‐ligand interaction between S. aureus bacterial protein and ligand 4 a, yielding a value of −7.63 kcal/mol for binding energy.

Assessing the market opportunities for pesticide-free vegetables in Bangladesh: consumers’ preferences and willingness to pay

The excessive use of fertilizers and pesticides in agriculture poses threats to ecosystems and human health. To mitigate these risks, researchers have turned their attention to pesticide-free production methods. This study aimed to assess the market opportunities for pesticide-free vegetables (PFVs) through determining consumers’ preferences and willingness to pay (WTP), along with identifying factors influencing consumers' WTP decision in three major cities of the country, namely Dhaka, Khulna, and Mymensingh. Employing a multistage cluster sampling method, 150 samples were selected and data collection utilized the android-based app Kobo through direct interviews. The contingent valuation method (CVM) and double-bounded dichotomous choice model (DBDC) were used to estimate WTP and preferences while ordered logit model were employed to identify influencing factors. The findings indicated that over 90% of consumers in Mymensingh and over 92% in Dhaka and Khulna were willing to pay a premium for pesticide-free tomatoes and brinjal. Additionally, more than 18% and 19% of consumers expressed willingness to pay a 20% premium for brinjal and tomato, while over 39% were willing to pay for both a 10% premium. The study revealed that factors such as age, sex, education, household size, and income significantly influenced consumers’ WTP decisions, with age showing a negative correlation, while education and income were positively associated with interest in consuming pesticide-free vegetables. Overall, the study underscores the increasing demand for pesticide-free vegetables in urban areas, emphasizing the need for governments and stakeholders to develop supportive policies for sustainable market development. Res. Agric. Livest. Fish. Vol. 11, No. 1, April 2024: 69-77

Strategies for Enhancing Plant Immunity and Resilience Using Nanomaterials for Sustainable Agriculture.

Research on plant-nanomaterial interactions has greatly advanced over the past decade. One particularly fascinating discovery encompasses the immunomodulatory effects in plants. Due to the low doses needed and the comparatively low toxicity of many nanomaterials, nanoenabled immunomodulation is environmentally and economically promising for agriculture. It may reduce environmental costs associated with excessive use of chemical pesticides and fertilizers, which can lead to soil and water pollution. Furthermore, nanoenabled strategies can enhance plant resilience against various biotic and abiotic stresses, contributing to the sustainability of agricultural ecosystems and the reduction of crop losses due to environmental factors. While nanoparticle immunomodulatory effects are relatively well-known in animals, they are still to be understood in plants. Here, we provide our perspective on the general components of the plant's immune system, including the signaling pathways, networks, and molecules of relevance for plant nanomodulation. We discuss the recent scientific progress in nanoenabled immunomodulation and nanopriming and lay out key avenues to use plant immunomodulation for agriculture. Reactive oxygen species (ROS), the mitogen-activated protein kinase (MAPK) cascade, and the calcium-dependent protein kinase (CDPK or CPK) pathway are of particular interest due to their interconnected function and significance in the response to biotic and abiotic stress. Additionally, we underscore that understanding the plant hormone salicylic acid is vital for nanoenabled applications to induce systemic acquired resistance. It is suggested that a multidisciplinary approach, incorporating environmental impact assessments and focusing on scalability, can expedite the realization of enhanced crop yields through nanotechnology while fostering a healthier environment.

Diagnosis and treatment of oral mucus lesions: case series of four patients

One of the pressing issues in dermatovenerological and stomatological practices pertains to diseases of the oral mucosa. This challenge is primarily attributed to the intricacies associated with diagnosing these conditions in their early stages, the resemblance of clinical presentations to various oral mucosal lesions, and the potential lack of awareness among healthcare practitioners. Diseases such as lichen planus, herpes simplex, erythema multiforme exudative with skin lesions and vulgar pemphigus are characterized by a particularly persistent long-term course, thereby reducing the patient's ability to work and the overall quality of life. A multitude of endogenous and exogenous factors can influence the state of the oral mucosa. Endogenous factors encompass genetic predisposition, endocrine disturbances, carbohydrate metabolism disorders, neurogenic disorders, autoimmune conditions and infectious diseases. Exogenous factors, as a rule, include taking medications (non-steroidal anti-inflammatory drugs, antibiotics), galvanism, excessive use of alcohol and narcotic substances, and others. It should be noted that the condition of the oral mucosa is also influenced by a wide range of different trigger factors. The narrative delves into the complexities of diagnosing these conditions early on and explores the challenges inherent in devising effective treatment strategies.

Astaxanthin-mediated Nrf2 activation ameliorates glucocorticoid-induced oxidative stress and mitochondrial dysfunction and impaired bone formation of glucocorticoid-induced osteonecrosis of the femoral head in rats

BackgroundOsteonecrosis of the femoral head caused by glucocorticoids (GIONFH) is a significant issue resulting from prolonged or excessive clinical glucocorticoid use. Astaxanthin, an orange-red carotenoid present in marine organisms, has been the focus of this study to explore its impact and mechanism on osteoblast apoptosis induced by dexamethasone (Dex) and GIONFH.MethodsIn this experiment, bioinformatic prediction, molecular docking and dynamics simulation, cytotoxicity assay, osteogenic differentiation, qRT-PCR analysis, terminal uridine nickend labeling (TUNEL) assay, determination of intracellular ROS, mitochondrial function assay, immunofluorescence, GIONFH rat model construction, micro-computed tomography (micro-CT) scans were performed.ResultsOur research demonstrated that a low dose of astaxanthin was non-toxic to healthy osteoblasts and restored the osteogenic function of Dex-treated osteoblasts by reducing oxidative stress, mitochondrial dysfunction, and apoptosis. Furthermore, astaxanthin rescued the dysfunction in poor bone quality, bone metabolism and angiogenesis of GIONFH rats. The mechanism behind this involves astaxanthin counteracting Dex-induced osteogenic damage by activating the Nrf2 pathway.ConclusionAstaxanthin shields osteoblasts from glucocorticoid-induced oxidative stress and mitochondrial dysfunction via Nrf2 pathway activation, making it a potential therapeutic agent for GIONFH treatment.

Sleep Hygiene Intervention Improves Sleep Time and Duration in High School Students

Abstract Objective To evaluate the effects of a sleep hygiene education program for adolescents to address excessive daytime sleepiness, insomnia, and sleep-related behaviors. Materials and Methods The participants were 98 high school students from the 11th and 12th grades. A day-long health promotion action was conducted at their school science fair to disseminate relevant information about sleep disorders. Social status and sleep perception and habits were evaluated through the following validated questionnaires: the Brazilian Economic Classification Criteria, a questionnaire on sleep habits for adolescents, the Pediatric Daytime Sleepiness Scale, the Insomnia Severity Index, and the Sleep Time-Related Information and Communication Technology questionnaire. The questionnaires were administered before the fair and 12 months after participation, and the results before and after the intervention were compared by means of the Student t-test and the Fisher exact test. For the correlations involving the results, the Pearson correlation was used with the level of significance of p &lt; 0.05. Results A qualitative reduction in poor bedtime habits was observed after the intervention, such as watching television and leaving a cell phone on with sound. A higher frequency of use of electronic devices can affect school performance and contribute to later bedtime on weekends (p &lt; 0.001). Excessive daytime sleepiness was correlated with the excessive use of electronic devices (p = 0.017). Conclusion Some habits changed after the awareness intervention. There was an impact of the use of electronic devices on academic performance, on the regularity of bedtimes, and on excessive daytime sleepiness. We emphasize the importance of health education programs with sustainable actions following actual changes in behavior.

Content in the Red Cup: Perceived Implication of Substance Use in Nigeria Hip Hop Music Video Performance

Music is an essential part of mass communication that aids the stimulation of concrete information in a lighter mood. The current trend of cup usage by artist in Nigeria music video performance has presently increased the imitation among the upcoming artist and youth. Substance abuse can be referred to excessive use of psychoactive drugs. This study investigates use of red cup and perceived implication of substance abuse in Nigeria music video performance. The study made use of quantitative research approach. The research design for this study is survey method, questionnaires were used gather data needed for the study. Simple random sampling was adopted to select 200 respondents among students of the department of performing art and film studies and mass communication and media technology Lead City University, Ibadan. The result obtained were analysed and presented in percentage tables. The study found out that most of the red cups found in Nigeria Music Video contain drug substance. The study also revealed that use of substance during performance can lead to drug addiction and other related effects. The study concluded that the content in red cup of Nigeria music videos contain drug substance, this can lead to addiction and immoral imitation that can influence viewers and upcoming artist. The study recommends that artist should reduce the use of red cup as it sends negative signals to the viewers who see them as role model and mentor, also, artist should avoid intake of drugs substance and alcohol during performance in other to retain originality in their music performance.

Production of sawdust and chicken fat briquettes as an alternative solid fuel

This study focuses on converting underutilized or discarded sawdust from Eucalyptus grandis (EG) and Pinus elliottii (PE), along with roasting chicken oil (RCO) and chicken visceral oil (CVO), into alternative briquettes. Employing heuristic methods and branch-and-bound techniques, 27 experiments were conducted based on a central composite design (CCD), including two central point repetitions. The energy density (ED) of the briquettes was evaluated immediately after production (EDi) and three months later (EDf). The higher heating value (HHV), thermogravimetric analysis (TGA) with its first derivative, and cost of each briquette were also characterized. Increasing chicken oil content improved HHV, initial ED, reduced ash content, and enhanced thermal degradation performance. However, exceeding 15% oil content caused wastage during pressing. Excessive CVO use is cost-prohibitive unless produced by the poultry industry. The optimal briquette was obtained in experiment 9 with 21.25% EG, 63.75% PE, 3.75% CVO, and 11.25% RCO by mass. Structural neighbors were identified based on this composition. Sawdust type and particle size had minimal impact on the results.

The prosecutor's office, pretrial detention and the principle of objectivity: legal challenges, criteria and measures

The general objective of this study is to critically review how the excessive demand, by the prosecutor's office as head of public criminal action, in Ecuador, with respect to the application of the precautionary measure of preventive detention, on the occasion of hearings for the formulation of charges and/or classification of flagrancy, ignores and violates the principle of objectivity, pointing out the excessive use of this measure as a research problem. For this purpose, a qualitative approach methodology is used, under exegetical, analytical and inductive methods, using as a data collection technique the interview with three Ecuadorian prosecutors. As a result, it was found that, in effect, the request for this precautionary measure ignores and violates the principle of objectivity, given that the prosecutors take into account elements such as the prevention of new crimes and the protection of victims to base it, moving away from the criteria stipulated by national regulations, which violates this and other procedural principles and, consequently, violates the accused's right to due process. Keywords: