This research demonstrated that an aerobic fluidized bed reactor can be operated either to maximize substrate removal, to minimize solids production or to treat intermittently produced wastewater. The operational mode is controlled by the quantity of biomass attached to the media. A thinner biomass encourages endogenous respiration and minimizes excess sludge production. Increasing biofilm thickness increases substrate removal efficiencies up to the point at which the diffusion of substrate, nutrients or oxygen becomes limiting. A thick biofilm was found to remain active even after an extended non-loading period, most likely because of endogenous respiration. Upon the introduction of substrate after the non-loading period, the substrate was immediately removed. This indicates the potential of using a fluidized bed reactor for applications that produce only intermittent wastewater flows, such as those found in many industries and hazardous waste remediation systems.