Excessive Feeding Research Articles

Mariculture is a double-edged sword in China

China now accounts for over 2/3 of the world's production of marine-based seafood. Chinese mariculture has contributed to the stability of the global food supply, but millions of tons of excess feed and drugs are threatening the sustainability of the waters in which it is conducted. Over 26% of the excess nitrogen in China's waters is likely a result of shrimp production alone, worsening eutrophication and the resulting ‘red tide’ in China. While some Chinese operations require the destruction of carbon-rich wetlands, the sector as a whole can be considered a net CO2 sink at the immediate time of harvest; each year, Chinese mariculture extracts the same quantity of CO2 from the atmosphere as 5000 km2 of forest. Novel technical practices, improved management of the harvested products in the food versus industrial supply chain, optimized sea-use and land-use plans, and the encouragement of participatory decision-making by provincial entities offer some promise to ensure the sustainability of this global public resource – but the national Chinese government will have to step up to address the challenges.

Abnormal neurological soft signs in babies born to smoking mothers were associated with lower breastfeeding for first three months

We examined associations between neurological alterations in infants born to smoking mothers and breastfeeding success at discharge and three months of age. This 2016 study compared 35 normal weight infants born to smoking mothers at 37-41weeks and 35 matched controls born to non-smoking mothers at the Maternity Hospital of Careggi University, Florence, Italy. Neonatal behaviour was evaluated using the neurological soft signs (NSS) component of the Graham-Rosenblith Scale. Breastfeeding variables were measured using the LATCH score that covers: breast latching, audible swallowing, type of nipple, mother's comfort and help they needed to hold their baby to their breast. A questionnaire on excessive crying and feeding was distributed at discharge, and further data were collected during a three-month telephone interview. At discharge, the infants born to smoking mothers had a significantly lower LATCH score and significantly poorer performance on several items of the NSS component than the controls. The LATCH score and number of NSS were inversely proportional. At the three-month follow-up only 57.1% of the smoking group infants were breastfeeding compared with 87.5% of the control infants (p<0.01). Infants with smoking mothers displayed altered neurobehavioural profiles and had a difficult start to breastfeeding.

Fuzzy Self-Tuning PID-Based Intelligent Control of an Anti-Wave Buoy Data Acquisition Control System

In aquaculture, water quality is influenced by excessive feeding, a high breeding density, the species composition and the distribution of algae species. Traditional buoys can only collect water quality data at a fixed depth, and it is impossible to measure the water quality at different depths in the water column. Sea waves are common in the open ocean and influence data acquisition systems via wave forces, which affect the accuracy of data collection. Considering the above problems, a data acquisition control system with automatic lifting is designed. The underwater acquisition system collects water quality data and sends the data to a microcontroller. The microcontroller sends a displacement control signal to a PLC according to the processed data. The PLC automatically develops the corresponding acceleration and deceleration operating curves according to the displacement. A fuzzy PID self-tuning control method is used to accurately control the speed and steering of the stepping motor, and an IIR digital filter is designed to reduce the interference from sea waves. The simulation results show that the stepping motor responds quickly, the speed of the stepping motor is stable, and the data acquisition time is reduced based on the proposed algorithm. Moreover, wave interference is largely mitigated, and the system is generally stable.

Additional feeding during late gestation improves initial litter weight of lactating sows exposed to high ambient temperature

ABSTRACT The present study investigated the impacts of additional feeding (AF; containing 16% excess feed than control diet in the last two weeks of gestation) during late gestation period and different backfat thickness (BFT) including low (<20 mm) and high (≥20 mm) backfat on reproductive performance, litter size, blood metabolites, hormonal profiles, colostrum, and milk composition of sows from day 90 of gestation to farrowing, and from farrowing to weaning during summer (average 28.3 °C). Fifty-four crossbred sows (Yorkshire × [...]

Evaluation of a polyherbal formulation for the management of wet litter in broiler chickens: Implications on performance parameters, cecal moisture level, and footpad lesions.

Objective:The study was carried out to develop a wet litter model with magnesium chloride to assess the effectiveness of a polyherbal formulation (PHF) on growth performance, litter and cecal moisture (LCM) level, cecal consistency (CC) score, and footpad lesions (FPLs) score in Ross 308 broiler chickens.Materials and Methods:1,200 one-day-old chicks were assigned into five groups: normal control, negative control [NTC; treated with 1.7% magnesium chloride hexahydrate (MgCl2.6H2O)], and three treatment groups, T1, T2, and T3, where 750, 1,000, and 2,000 gm/ton of PHF, respectively, were supplemented. All the groups were fed a basal diet until day 7. However, the NTC and treatment groups were fed a diet with MgCl2 from days 8 to 42.Results:The addition of MgCl2 for 35 days worsened the growth performance traits in broilers and induced wet litter problems (FPL, high LCM, and poor CC) in the NTC group. However, PHF (750, 1,000, and 2,000 gm/ton) ameliorated the negative effect of a diet with MgCl2 on growth performance and wet litter problems, but a better response with respect to LCM and CC was observed in 2,000 gm/ton of PHF group, followed by that in 1,000 gm/ton of PHF group and 750 gm/ton of PHF group on day 42.Conclusion:The wet litter broiler model was developed through excessive feeding of MgCl2, which caused the performance parameters to worsen and the emergence of problems associated with the wet litter. Supplementation with PHF ameliorated these problems and, therefore, it can be used for the management of wet litter in poultry.

Synthesis of Alkyne-functionalized Polymers via Living Anionic Polymerization and Investigation of Features during the Post-“thiol-yne” Click Reaction

“Thiol-yne” click reaction has already been widely applied in synthesis and modification of new polymer structures or novel materials due to its specific features. However, in most studies, only chain-end strategy was employed when using the di-addition feature of thiol-yne reaction, thus the in-chain di-addition strategy could endow us with a broader space to develop the synthesis of advanced polymers. Therefore, in this paper, the features of in-chain mono- and di-addition were investigated when modifying the alkyne-functionalized polymers to prepare grafted polymers via thiol-yne click reaction. The results showed that it is almost impossible to obtain the in-chain di-adducts even under excess feeding of chain-end thiol-functionalized grafts, while only the in-chain mono-adducts could be obtained efficiently. Further researches investigated that the controlled grafting could be encountered when carrying out the thiol-yne click reaction between chain-end alkyne-functionalized polystyrenes and chain-end thiol-functionalized polystyrenes under proper feedings. Therefore, the effect of steric-hindrance might be the primary reason for the alternative grafting via thiol-yne click reaction between in-chain and chain-end alkyne-functionalized polymers.

English

Dietary wood charcoal can be a potential low-cost feed supplement for the improvement of performance in broiler chicken production, while reducing loss of nutrients through birds’ excreta. However, it has no nutritive value and excess feeding may lead to constipation and thereby hamper birds’ production performance. An experiment was conducted with a total of 24 male broiler chickens which were subjected to a commercial broiler finisher diet with 0, 1.5, 3, and 6% wood charcoal (on a dry matter basis), respectively. This was to ascertain the level of dietary wood charcoal that can be included in a commercial broiler feed without negative effects on production performance of broiler chickens, nutrient utilization and losses through birds’ excreta under adverse climatic conditions in Northern Ghana. Birds’ feed consumption, body weight gain, as well as excreta quality were assessed for four days. Results showed that dietary wood charcoal can replace up to 6% of a commercial broiler chicken feed without negative effects on growth performance, nutrient utilization and excreta consistency, while reducing the phosphorous concentration in broiler excreta. Future research should analyze the long-term effect of feeding charcoal on performance and health of laying hens. Key words: Broiler chickens, dietary wood charcoal, nutrient utilization, production performance, excreta quality.

NOx Emissions from Regenerator of Calcium Looping Process

The calcium looping (CaL) process usually employs a dual-fluidized bed (DFB) solid circulating unit. In the regenerator (calcinator or calciner), decomposition of CaCO3 proceeds. To supply the heat of decomposition, oxyfuel combustion of coal is conducted. However, since coal contains nitrogen, the NOx formation occurs during oxyfuel combustion. Because of the fact that NOx formation and destruction during combustion of solid fuels in a fluidized bed is a complex process, a predictive approach of NOx emissions has not yet been sufficiently recognized, especially during oxyfuel combustion conditions in the CaL systems. The paper introduces a regression-based method for the prediction of NOx emissions from a CaL DFB experimental unit. Effects of fuel type, excess oxygen feed, and NO addition to primary or secondary feed gas on NOx emissions in the regenerator were evaluated. The presented way constitutes a straightforward method to run a complementary technique in relation to other methods of data handling,...

Diet-Induced Growth Is Regulated via Acquired Leptin Resistance and Engages a Pomc-Somatostatin-Growth Hormone Circuit

SUMMARYAnorexigenic pro-opiomelanocortin (Pomc)/alpha-melanocyte stimulating hormone (αMSH) neurons of the hypothalamic melanocortin system function as key regulators of energy homeostasis, also controlling somatic growth across different species. However, the mechanisms of melanocortin-dependent growth control still remain ill-defined. Here, we reveal a thus-far-unrecognized structural and functional connection between Pomc neurons and the somatotropic hypothalamo-pituitary axis. Excessive feeding of larval zebrafish causes leptin resistance and reduced levels of the hypothalamic satiety mediator pomca. In turn, this leads to reduced activation of hypophysiotropic somatostatin (Sst)-neurons that express the melanocortin receptor Mc4r, elevated growth hormone (GH) expression in the pituitary, and enhanced somatic growth. Mc4r expression and αMSH responsiveness are conserved in Sst-expressing hypothalamic neurons of mice. Thus, acquired leptin resistance and attenuation of pomca transcription in response to excessive caloric intake may represent an ancient mechanism to promote somatic growth when food resources are plentiful.

Prevalence and magnitude of acidosis sequelae to rice-based feeding regimen followed in Tamil Nadu, India.

Background and AimIn Tamil Nadu, a southern state of India, rice is readily available at a low cost, hence, is cooked (cooking akin to human consumption) and fed irrationally to cross-bred dairy cattle with poor productivity. Hence, a study was carried out with the objective to examine the prevalence of acidosis sequelae to rice-based feeding regimen and assess its magnitude.Materials and MethodsA survey was conducted in all the 32 districts of Tamil Nadu, by randomly selecting two blocks per districts and from each block five villages were randomly selected. From each of the selected village, 10 dairy farmers belonging to the unorganized sector, owning one or two cross-bred dairy cows in early and mid-lactation were randomly selected so that a sample size of 100 farmers per district was maintained. The feeding regimen, milk yield was recorded, and occurrence of acidosis and incidence of laminitis were ascertained by the veterinarian with the confirmative test to determine the impact of feeding cooked rice to cows.ResultsIt is observed that 71.5% of farmers in unorganized sector feed cooked rice to their cattle. The incidence of acidosis progressively increased significantly (p<0.05) from 29.00% in cows fed with 0.5 kg of cooked rice to 69.23% in cows fed with more than 2.5 kg of cooked rice. However, the incidence of acidosis remained significantly (p<0.05) as low as 9.9% in cows fed feeding regimen without cooked rice which is suggestive of a correlation between excessive feeding cooked rice and onset of acidosis. Further, the noticeable difference in the incidence of acidosis observed between feeding cooked rice and those fed without rice and limited intake of oil cake indicates that there is a mismatch between energy and protein supply to these cattle. Among cooked rice-based diet, the incidence of laminitis increased progressively (p<0.05) from 9.2% to 37.9% with the increase in the quantum of cooked rice in the diet.ConclusionThe study points out the importance of protein supplementation in rice-based feeding regimen to set right the mismatched supply between nitrogen and fermentable organic matter in the rumen. This research has practical implications for animal health, welfare, nutrition, and management

Prospective study of enhanced recovery after surgery protocol in children undergoing reconstructive operations

Enhanced recovery after surgery (ERAS) protocol is a set of peri-operative strategies to increase speed of recovery. ERAS is well established in adults but has not been well studied in children. The purpose of the current study was to establish the safety and efficacy of an ERAS protocol in pediatric urology patients undergoing reconstructive operations. It was hypothesized that ERAS would reduce length of stay and decrease complications when compared with historical controls. Institutional Review Board approval was obtained to prospectively enroll patients aged <18 years if they had undergone urologic reconstruction that included a bowel anastomosis. ERAS included: no bowel preparation, administration of pre-operative oral carbohydrate liquid, avoidance of opioids, regional anesthesia, laparoscopy when feasible, no postoperative nasogastric tube, early feeding, and early removal of intravenous fluids (IVF). Recent (2009-2014) historical controls were propensity matched in a 2:1 ratio on age, sex, ventriculoperitoneal shunt status and whether the patient was undergoing bladder augmentation. Outcomes were protocol adherence, length of stay (LOS), emergency department (ED) visits, re-admission within 30 days, re-operations and adverse events occurring within 90 days of surgery. A total of 26 historical and 13 ERAS patients were included. Median ages were 10.4 (IQR 8.0-12.4) and 9.9 years (IQR 9.1-11), respectively (P=0.94) (see Summary Table). There were no significant between-group differences in prior abdominal surgery (38% vs 62%), rate of augmentation (88% vs 92%) or primary diagnosis of spina bifida (both 62%). ERAS significantly improved use of pre-operative liquid load (P<0.001), avoidance of opioids (P=0.046), early discontinuation of IVF (P<0.001), and early feeding (P<0.001). Protocol adherence improved from 8/16 (IQR 4-9) historically to 12/16 (IQR 11-12) after implementation of ERAS. LOS decreased from 8 days to 5.7 days (P=0.520). Complications of any grade per patient decreased from 2.1 to 1.3 (OR 0.71, 95% CI 0.51-0.97). There were fewer complications per patient across all grades with ERAS. No differences were seen in emergency department (ED) visits, re-admissions and re-operations. Implementation improved consistency of care delivered. Tenets of ERAS that appeared to drive improvements included maintenance of euvolemia through avoidance of excess fluids, multimodal analgesia, and early feeding. ERAS decreased length of stay and 90-day complications after pediatric reconstructive surgery without increased re-admissions, re-operations or ED visits. A multicenter study will be required to confirm the potential benefits of adopting ERAS.

Причини та наслідки дефіциту йоду у харчуванні жінок і дітей

Iodine is an essential micronutrient, which is necessary for the thyroid hormones production. Its insufficient or excessive feeding violates the thyroid function. The best way to get iodine is a balanced nutrition that contains a sufficient amount of iodine'rich foods (seafood, milk and dairy products). For the countries, in which the population is suffering from the iodine deficiency in food, the optimal strategy for its normalization is the use of iodized salt on a regular basis for cooking in households, food outlets and the food industry. Under conditions of insufficient food intake of iodine for the high'risk of iodine deficiency group (children, adolescents, pregnant women, lactating mothers), it is possible to use dietary supplements and drugs with physiological content of iodine.

Intensity of growth and development of rainbow trout using feeds Aller Aqua and Aquafeed Fischfutter

Due to the rapid development of feed since the early 1990's, efficiency has increased dramatically. The effectiveness is achieved by increasing energy levels, reducing the level of carbohydrates and balancing various nutrients that meet the needs of fish. Improvement of efficiency should be taken into account when determining the amount of ration feeding. The economic efficiency of growing fish depends largely on proper feeding. The feed should be dosed depending on its formulation and size, the physiological state of the fish, the temperature of water and the content of oxygen in it. Salmon fish are demanding oxygen treatment. The content of oxygen in water should be at least 7 mg/l. At a lower oxygen level, the growth of fish is suppressed, and the effectiveness of food is reduced. The optimum temperature of water for growing salmon fish is 14–18 °С. Excessive feeding leads to unproductive feed costs and water pollution, insufficient feeding leads to incomplete realization of potential opportunities for the rate of growth of fish. The quantity of the daily ration is established taking into account the temperature of water and the mass of fish. Depending on their state, activity and conditions of maintenance, it can fluctuate within ± 20% of the norm. In addition, high fertility densities, unsanitary conditions, illness, low water exchange rates, low oxygen content in water affect the efficiency of feeding in trout farms. Due to the fact that the cost of forage for trout is up to 60% of all costs for its cultivation, the problem of it rational use and saving is at the forefront. When feeding trout it is necessary to take into account that the size of granules for young fish in the length of 4–20 cm should be from 2.2 to 2.6% of the body length (according to Smith), which corresponds to the size of the mouth and the distance between the gill stamens in rainbow trout. Fish is best fed often in small portions. The smaller the fish, the more often feeding. Going on a feed of another size or type should be gradually over 3–5 days. If necessary, it is necessary to use special therapeutic granulated food or a mixture of therapeutic ingredients to add to the wet granules. It is necessary to create optimal conditions for growing and to carry out constant monitoring of water temperature and its fluctuations, oxygen content in water, pH, atmospheric pressure, etc. It is necessary to follow the rules of storage of forages, taking into account that the quality of feed after being stored for more than 3 months is deteriorating. This happens. It may be caused by poor water quality or disease. In order for the fish to get used to a particular feeding regime, it is recommended to do feed it at the same time. Today, the diversity of feeds allows you to get a better feed rate, fast growth and a minimal impact on the environment under different conditions, since the need of fish in nutrients is fully covered by feeds (energy, protein, minerals and vitamins). The use of Aller Aqua feed guarantees receiving of a strong, healthy fish, thus providing good economic performance to farms. The basis of the research is the analysis of the nutritional value of the feeds of the company Aquafeed Fishsfutter of the German producer and Aller Aqua of the Danish company in the production conditions on the farm of the Western Fish Company PP of Peremyshlyany district, Lviv region. Also we should study fodder, their composition and carry out an objective assessment. It is known that fodder of foreign producers are characterized by higher energy value due to increased fat content, which is achieved by application of the appropriate technology and equipment for vacuum desiccation. For example, Aller Aqua's salmon-based compound feed for protein levels 40–46% contains 15 to 32% lipids and 23–24 MJ/kg of gross energy. Due to the high energy value, these feeds allow to provide the growth of fish at low feed costs. The aim of the work was to analyze the impact of different producers of feed fodder for commodity groups of trout on their growth and the development and receipt of quality commodity products in short terms.

Effects of Monomer Feed Amounts on Solution Properties and Solution Morphologies for a Comb-like Ter-polymer

ABSTRACTAn acrylamide(AM)-based ter-polymer (PAAB) containing sodium 2-acrylamido-2-methylpropane sulphonate (NaAMPS) and p-vinylbenzyl-terminated octylphenoxy poly(ethylene oxide) (VBE, polymerization degree: 24) was synthesized by an aqueous free-radical copolymerization. In order to obtain a PAAB polymer with both good thickening properties and surface and interfacial properties in brine solutions for enhanced oil recovery (EOR), the influences of the NaAMPS and VBE feed amounts on the intrinsic and apparent viscosities and the surface and interfacial tensions of the PAAB polymer in aqueous solutions were investigated. The optimum NaAMPS feed amount was 9 mol%; an excessive feed amount interfered with the aggregations of the PAAB macro-molecules in aqueous solutions and on the surfaces of aqueous solutions and the interfaces between aqueous solutions and kerosene. In addition, for this NaAMPS feed concentration, the PAAB polymer showed the highest apparent viscosities and the lowest surface and interfacial tensions in 5 g L−1 NaCl for a VBE feed amount of 1.1 mol%. The mechanism giving rise to the solution properties for this polymer was examined. For this purpose, the effect of the polymer concentration on the supra-molecular structures of the PAAB polymer in water and in 5 g/L NaCl, were investigated by ultraviolet spectral analysis using pyrene as a probe. A transmission electron microscope (TEM) was also used to observe the morphologies of the associated structures in aqueous solutions to explore the correlation between the solution properties and solution structures for PAAB; expanded polymer chain bundles were formed at a polymer concentration of 0. 4 g L−1, higher than the critical micelle concentration (0. 3 g L−1).

Increase the hosting capacity of four-wire low-voltage supply network for embedded solar generators by optimising generator and load placement on the three supply phases

Australia has topped the world in the penetration of residential rooftop solar generation systems. These residential solar generation systems are connected to the low-voltage (LV) supply network, which is not designed for two-way flow of electricity. Localised voltage rises due to excess solar generation feeding energy back into the supply network is commonly experienced. Supply utilities do not generally select the phases for the connection of solar generators. When load/generation is not balanced between the three LV supply phases, phase voltage unbalance arises as a result, which aggravates the phase voltage condition due to neutral voltage shift. Balancing loads and solar generation on each phase is quite complex due to the stochastic nature of loads and solar generation. This study proposes an allocation methodology based on minimising voltage unbalance on the supply nodes, subject to the constraint that voltages on each supply node/bus are within the regulatory limits. The effectiveness of the approach is illustrated by computer modelling studies implemented in MATLAB© and Open Distribution System SimulatorTM, using load and network data of a real-LV network in Australia.

The use of large databases to inform the development of an intestinal scoring system for the poultry industry

There is increasing interest among the poultry industry to develop a comprehensive index that can be used to evaluate overall intestinal health and impact on production performance. The Intestinal Integrity (I2) index is a quantitative measurement tool used to assess the intestinal health of flocks that use the Health Tracking System (HTSi), a global surveillance system developed by Elanco Animal Health that captures flock-level information on health and performance. To generate an I2 index score for a flock, the presence of 23 intestinal health conditions is assessed and recorded, then entered into a mathematical equation. The objective of this study was to use data from the HTSi dataset to investigate the association between health conditions contained within the I2 index and five performance outcomes: average daily gain (ADG), mortality during the first week, feed conversion ratio (FCR), European Production Efficiency Factor (EPEF), and percent livability. At the time of analysis, the HTSi dataset contained information from the years 2006–2015 on 921,646 individual bird necropsy records from over 153,576 flocks at 1,570 broiler production flows across 53 countries. Flock-level production data used for this study were available for a subset of this population, 33,212 total flocks representing 6 US and 4 UK production flows. A separate multivariable linear or logistic regression model, with farm as a random effect, was built for each of the five outcomes mentioned above. All models controlled for clustering of flocks within production flows. Significant associations were found between key performance indicators and ten intestinal conditions (gross E. acervulina, gross E. maxima, microscopic E. maxima, gizzard erosions, roundworms, excessive intestinal fluid, thin intestines, excessive intestinal mucus, feed passage, and necrotic enteritis) and two management parameters (production flow and down time). Results from this study demonstrate that large databases, such as the HTSi database, can be used to inform and evaluate changes that can optimize intestinal health, and hence welfare, productivity, and sustainability of the poultry industry. In addition, large databases can be used for monitoring and benchmarking intestinal health of poultry and informing the development of innovative indices such as the I2 index for scoring intestinal health and providing routine data for its calculation.

Synthesis of Aliphatic Carbonate Macrodiols and Their Application as Sustainable Feedstock for Polyurethane.

High-molecular-weight poly(propylene carbonate) (PPC) [number-average molecular mass (Mn): 80 000–100 000] is readily alcoholized into PPC macrodiols in the presence of 1,2-propanediol (PDO), 1,4-butanediol (BDO), or 1,6-hexanediol (HDO). The high-molecular-weight PPC and small amount of diols, such as PDO, BDO, or HDO, were stirred at elevated temperatures to convert the extremely viscous high-molecular-weight polymer to low-molecular-weight macrodiols with gel permeation chromatography-measured Mn of about 3000 Da. The chopping reaction of the high-molecular-weight PPC was studied in detail, such as the influences of the catalyst residue, the kinds of alcoholysis agents, reaction temperature, and time. The reaction mechanism of alcoholysis is proposed according to the experimental results. The results indicate that the presence of a trace residue of zinc catalyst (Zn-G-III) in PPC, excess diol feeding, and higher temperature can accelerate the alcoholysis. Moreover, different diols can produce different PPC macrodiols with varying end-capping. Finally, polycarbonate ether urethane can be successfully synthesized using as-synthesized PPC macrodiols and poly(propylene glycol) (Mn ≈ 3000) as the soft segment and 4,4′-diphenylmethane diisocyanate or BDO as the hard segment. The full evaluation for the synthesized PPC macrodiols demonstrates their potential applications in the polyurethane industry.

2016년도 제주지역 양식 넙치(Paralichthys olivaceus)에서 분리된 어병세균의 tetracycline 내성유전자 분포

Aquaculture practices to ensure greater production, such as high density breeding and excessive feeding, are become stressors that raise the prevalence of diseases. Accordingly, increasingly large volumes of antibiotics are used more frequently each year. Long term use antibiotics can generate resistant bacteria, which interrupt treatments and cause a potential transfer to human bodies. Thus, antibiotic resistance is of importance in public health. Tetracycline (Tc) is one of the typical medicines used in the aquaculture drugs, which has a wide range of application including gram-positive and gram-negative bacteria. In the examination of 153 strains isolated from olive flounder (Paralichthys olivaceus) farms located in Jeju in 2016, it turned out that a total of 84 strains were resistant to Tc or oxytetracycline (OTC). The extent to which the strains are resistant to Tc and OTC was confirmed through MIC test, mostly within the range of 25 to 100 ㎍/㎖. Twelve different types of tet genes were detected using single and multiplex PCR in the 84 Tc-resistant strains. The PCR was used to find tet(K), tet(M), tet(O), and tet(S), which are known to exist primarily in gram–positive strains. According to the results, tet(S) is the most dominant gene in 49 strains of Streptococcus parauberis, accounting for 63.2%. And there were two strains that have two different types of resistant genes. The multiplex PCR was used to detect tet(A), tet(B), tet(C), tet(D), tet(E), and tet(G), which are commonly found in gram-negative strains. Each of tet(B), tet(D), and tet(B)&(M) was found in a strain presumed to be Vibrio sp., and only tet(D) was found in 10 Edwardsiella tarda strains.

A new integrated heat pump option for heat upgrading in Cu-Cl cycle for hydrogen production

A potential cascaded vapor compression heat pump is proposed to address the high temperature heat demand in the copper chlorine (Cu-Cl) thermochemical cycle for hydrogen production. The configuration studied is a cuprous chloride CuCl vapor compression heat pump cascaded with a biphenyl (C6H5)2 heat pump. Such cascaded heat pumps is meant to upgrade heat from nuclear power plants with a heat input temperature of approximately 300°C or industrial waste heat to meet the Cu2OCl2 decomposition reactor heat demand. Energy and exergy analyses are performed to understand the performance of the heat pump. It is determined that the CuCl-biphenyl heat pump exhibits a high coefficient of performance for certain operating conditions relating to the compressor isentropic efficiency and the excess CuCl feed temperature. The base energetic and exergetic coefficient of performances of the CuCl-biphenyl heat pump are 1.76 and 1.15 respectively.

Study on the Mechanism of a Side Coupling Reaction during the Living Anionic Copolymerization of Styrene and 1-(Ethoxydimethylsilyphenyl)-1-phenylethylene (DPE-SiOEt).

A 1,1-diphenylethylene (DPE) derivative with an alkoxysilyl group (DPE-SiOEt) was synthesized. It was end-capped with poly(styryl)lithium (PSLi) and then copolymerized with styrene via living anionic polymerization (LAP) in a non-polar solvent at room temperature. The observed side coupling reaction was carefully investigated by end-capping the polymer. Changes in molecular weight support the plausibility of a mechanism involving living anionic species (PSLi or lithiated DPE-end-capped polystyrene, PSDLi) and the alkoxysilyl groups. Through a series of copolymerizations with different feed ratios, the kinetics of the side coupling reaction were also studied. The results showed that the side reactions could be controlled using an excess feed of DPE-SiOEt, a potentially useful strategy for the synthesis and application of well-defined alkoxysilyl-functionalized polymers via LAP.