Examination Of Punch Biopsies Research Articles

The morphological criteria of the course of the rosacea vascular form.

Background. Rosacea is a known skin disease of the central part of the face, affects people of both sexes and is manifested by the persistence of erythema . Modern literature sources allow attributing this disease to the group of angioproliferative diseases, which are based on the activation of inflammation and angiogenesis. Objective. The aim of the study was to determine the morphological diagnostic criteria for the prescription of the disease and the possible dependence of the histological features of the rosacea vascular forms on the age of the patients. Methods. A retrospective histological analysis of the material of 11 patients aged 21-63 years (mean 40 ± 12.49, median 36 years) with a clinical diagnosis of the vascular form of rosacea was performed in the work. Results. The authors structured the early and late histological characteristics of skin lesions in vascular forms of rosacea, identified the main significant diagnostic criteria for this subtype of the disease, and analyzed their dependence on the age of the patients. Conclusion. The vascular form rosacea or the erythematous-telangiectatic subtype of rosacea has a wide range of pathological features, which requires an individual diagnostic approach in the form of mandatory histological examination of punch biopsies to establish the rosacea subtype, the stage and the duration of the lesion.

Adaptation process of the skin graft to vaginal mucosa after McIndoe vaginoplasty

There are many methods of treatment of vaginal agenesis. In this study, patients who underwent McIndoe vaginoplasty were evaluated for development of vaginal mucosa. Vaginal pH measurements, smears, and punch biopsies were obtained from seven patients postoperatively at 3, 6, and 12 months after having McIndoe vaginoplasty. Neovaginal smears indicated no specific data for mucosal development at the 3rd and 6th month after the operation. However, Doderlein bacilli were determined at the 12th month. Histopathological examination of punch biopsies revealed dermal and subdermal thickening at the 3rd month, partial adaptation to mucosa at 6th month, and full adaptation to mucosa at 12th month. Also normal vaginal pH levels were recorded at the 12th month. As a result of the adaptation process, the split thickness skin graft acquired both histological and physiological characteristics of normal vaginal mucosa. Also, neovaginal epithelium was evaluated to be macroscopically similar to normal vaginal mucosa, 12 months after the operation.

Sebaceous hyperplasia mimicking lymphangioma

Sebaceous hyperplasia of forehead, presenting as pin-sized translucent vesicles in a young girl is presented. The lesion was clinically mistaken for lymphangioma circumscriptum. Microscopic examination of punch biopsy of skin helped to arrive at a proper diagnosis.

A longitudinal study by daily histological examination of punch biopsies in a patient with toxic epidermal necrolysis (TEN)

A longitudinal study by daily histological examination of punch biopsies in a patient with toxic epidermal necrolysis (TEN)