Background: “Nuvan” is a trading name for Dichlorvos (DDVP) organophosphate insecticide. Nuvan contains76% DDVP and is accessible in India. Nuvan Insecticide is highly misused as orally ingested in suicide attemptsand is one of the leading causes of poisoning deaths in Indian rural areas.Methods: In this case study, we have diagnosed the four poisoning deaths by Nuvan-dichlorvos insecticidethrough Post-Mortem examination and toxicological analysis. The gastro-intestinal tract (GIT) was grosslyexamined. The solvent extraction method is used for withdrawing pesticide content from GIT. Toxicologicalanalyses were performed using thin-layer chromatography (TLC) using a spraying reagentResults: A Fluorescent turquoise blue liquid content was found throughout the stomach to the small intestine.Content had a notable odor of volatile organic compounds (VOCs). The mucosal walls of the stomach were highlycongested and hemorrhagic. Toxicological analyses done for all four cases showed a confirmatory detection ofdichlorvos.Conclusions: The simple gross examination of gastrointestinal viscera during post-mortem is supportingdiagnostic evidence for orally administered Nuvan insecticide. Solvent extraction method is a suitable techniquefor the extraction of pesticides from aqueous biological matrices. Thin Layer Chromatography is a simple andinexpensive technique for chemical confirmation and DDVP was detected.