Exact Replication Research Articles

Bioarchitectonic Nanophotonics by Replication and Systolic Miniaturization of Natural Forms.

The mimesis of biological mechanisms by artificial devices constitutes the modern, rapidly expanding, multidisciplinary biomimetics sector. In the broader bioinspiration perspective, however, bioarchitectures may perform independent functions without necessarily mimicking their biological generators. In this paper, we explore such Bioarchitectonic notions and demonstrate three-dimensional photonics by the exact replication of insect organs using ultra-porous silica aerogels. The subsequent conformal systolic transformation yields their miniaturized affine 'clones' having higher mass density and refractive index. Focusing on the paradigms of ommatidia, the compound eye of the hornet Vespa crabro flavofasciata and the microtrichia of the scarab Protaetia cuprea phoebe, we fabricate their aerogel replicas and derivative clones and investigate their photonic functionalities. Ultralight aerogel microlens arrays are proven to be functional photonic devices having a focal length f ~ 1000 μm and f-number f/30 in the visible spectrum. Stepwise systolic transformation yields denser and affine functional elements, ultimately fused silica clones, exhibiting strong focusing properties due to their very short focal length of f ~ 35 μm and f/3.5. The fabricated transparent aerogel and xerogel replicas of microtrichia demonstrate a remarkable optical waveguiding performance, delivering light to their sub-100 nm nanotips. Dense fused silica conical clones deliver light through sub-50 nm nanotips, enabling nanoscale light-matter interactions. Super-resolution bioarchitectonics offers new and alternative tools and promises novel developments and applications in nanophotonics and other nanotechnology sectors.

How Power Affects Moral Judgments: The Presence of Harm to Life Modifies the Association between Power and Moral Choices

Lammers and Stapel reported that high power increases deontological (rule-based) moral thinking, and low power increases utilitarian (outcome-based) moral thinking. However, the dilemmas were mild and did not involve harm to life. Here, we examined whether the presence or absence of harm to life affects the moral decisions of powerholders. To help establish the replicability and validity of the effects of power on moral judgments in the absence of harm to life, we first performed an exact replication of a study conducted by Lammers and Stapel, and this experiment was followed up by a similar study in an organizational context in China (Studies 1 and 2). Studies 3 and 4 investigated whether power and the presence/absence of harm to life interacted with preferences for deontological versus utilitarian moral judgments. Power consistently triggered deontological thinking. However, power differences in moral reasoning only emerged when there was no harm to life. Harm prompted deontological responses among control and powerless individuals, which nullified differences across the power conditions. The findings demarcate the generalizability of the association between power and a moral thinking style.

ChatGPT's Gastrointestinal Tumor Board Tango: A limping dance partner?

ObjectivesThis study aimed to assess the consistency and replicability of treatment recommendations provided by ChatGPT 3.5 compared to gastrointestinal tumor cases presented at multidisciplinary tumor boards (MTBs). It also aimed to distinguish between general and case-specific responses and investigated the precision of ChatGPT's recommendations in replicating exact treatment plans, particularly regarding chemotherapy regimens and follow-up protocols. Material and methodsA retrospective study was carried out on 115 cases of gastrointestinal malignancies, selected from 448 patients reviewed in MTB meetings. A senior resident fed patient data into ChatGPT 3.5 to produce treatment recommendations, which were then evaluated against the tumor board's decisions by senior oncology fellows. ResultsAmong the examined cases, ChatGPT 3.5 provided general information about the malignancy without considering individual patient characteristics in 19% of cases. However, only in 81% of cases, ChatGPT generated responses that were specific to the individual clinical scenarios. In the subset of case-specific responses, 83% of recommendations exhibited overall treatment strategy concordance between ChatGPT and MTB. However, the exact treatment concordance dropped to 65%, notably lower in recommending specific chemotherapy regimens. Cases recommended for surgery showed the highest concordance rates, while those involving chemotherapy recommendations faced challenges in precision. ConclusionsChatGPT 3.5 demonstrates potential in aligning conceptual approaches to treatment strategies with MTB guidelines. However, it falls short in accurately duplicating specific treatment plans, especially concerning chemotherapy regimens and follow-up procedures. Ethical concerns and challenges in achieving exact replication necessitate prudence when considering ChatGPT 3.5 for direct clinical decision-making in MTBs.

The Grammar of Persuasion: A Meta-Analytic Review Disconfirming the Role of Nouns as Linguistic Cues of Subsequent Behavior

Recent discoveries suggest that nouns, as a word class, exhibit a greater capacity to model real-life behavior. In some previous studies participants prompted with a noun label rather than a semantically corresponding verb ( be a voter vs. vote) were more likely to vote. However, many attempts to replicate the original results have shown unsuccessful. To shed light on the effectiveness of subtle linguistic manipulations, we conducted a meta-analysis of 28 effect sizes ( N = 13,376), being an exact or conceptual replications of original studies. Although the average effect was significant ( g = 0.12), the concurrent diagnostics indicated a low trust in the replicability or stability of the effect. Based on that, we cannot conclude that the greater effectiveness of nouns as cues has been reliably demonstrated. We discuss theoretical prospects involving the verb-action link as well as the self-verification motives.

The scope and limits of implicit bias training: An experimental study with Swedish social workers

Implicit bias reduction has become an increasingly popular feature of so-called ‘diversity training’ in both public and private organizations. It remains popular, despite a lack of robust evidence suggesting that it is possible to accomplish lasting changes to individual implicit bias. In addition, previous research relies almost entirely on laboratory experiments; almost nothing is known about the scope of these findings. The present study aims to engage theoretically and empirically in this debate. An implicit bias reduction intervention developed by psychologist Patricia Devine will be tested and approached from a sociological perspective. The study is based on an experiment setting with social workers at 13 Swedish social assistance units. Beyond testing the scope of this particular intervention, the study highlights some of the methodological problems that come with the unrealistic standards of exact replication within the social sciences. The results show that the intervention increased the participants’ awareness of prejudice and implicit bias, but it did not reduce the participants’ implicit biases. The scientific and social policy consequences of these findings are discussed.

Advancing OSCM scientific knowledge by replicating empirical findings: Step‐by‐step procedure and illustration for transformative replication endeavors

AbstractReplication endeavors contribute to the accumulation of scientific evidence about previously reported findings and are crucial for scientific progress. Replication studies are, however, often discouraged and rarely published in the operations and supply chain management (OSCM) discipline. In this article, we offer a framework for replications consisting of two complementary tables. This framework recognizes two types of replications already defined in the literature (i.e., The Exact (EXT) Replication and the Methods‐Only (MTD) Replication) and adds to these two new types (i.e., the Bounded‐Conceptual‐Extension (BCE) Replication and the Transformative (TRF) Replication). The framework clarifies what constitutes replications, forms of replication endeavors, and their purposes. Importantly, we also differentiate replication endeavors from reproducibility tests, robustness checks, and post hoc analyses. Moreover, we describe a seven‐step procedure to guide the design, execution, and presentation of replication endeavors, illustrating these steps by conducting a TRF Replication that incorporates, at the same time, a BCE Replication and an MTD Replication of Polyviou et al. (2018). The proposed framework and seven‐step procedure hopefully motivate OSCM scholars to embrace replications as valuable scientific endeavors that can yield corroborating evidence to bolster confidence in previously reported findings and, better yet, provide new nuanced findings to advance precise scientific understanding of past and new OSCM phenomena.

Role of foreign banks in promoting financial inclusion: “A time series analysis of five permanent members of UN security council”

PurposeFinancial Inclusion is an important determinant of bringing inclusive growth which is promoted by the foreign bank entry as it increases the market competition and brings about greater outreach. Foreign Bank Entry has been considered an impediment towards domestic markets which is misunderstood. Foreign Banks not only bring higher assets but also provide credit to government-owned enterprises, Small and Medium Enterprises, and Micro Credit. Most of the developed countries have understood this phenomenon and have achieved significant financial inclusion by liberalizing their banking industry. It is essential to conceptualize and measure the magnitude of factors, determinants, and antecedents for foreign bank entry promoting financial inclusion. MethodologyAn empirical research methodology has been adopted using the Normality test, data diagnostics for Regression, Unit Root Test, Bounds Co-Integration Test, Long Run Coefficients, and ARDL to explore the determinants of the entrance of foreign banks to promote financial inclusion. Data has been taken from 5 developed countries, United Nations Security Council's permanent members with Veto Powers including (United Kingdom, United States, Russian Federation, France, and China) for 15 years from 2004–2018, making it a sample size of 75 (n = 75) for each of the eight variables under study. FindingsThe results confirm that the foreign bank entry has a significant effect at the level and 1st order difference on the financial inclusion. OriginalityThis study is the original work of the authors and is not the exact replication of any prior study; this study provides empirical evidence and discusses the literature on Financial Inclusion and Foreign Bank Entry.

Assessing the effects of pandemic risk on cooperation and social norms using a before-after Covid-19 comparison in two long-term experiments

How does threat from disease shape our cooperative actions and the social norms that guide such behaviour? To study these questions, we draw on a collective-risk social dilemma experiment that we ran before the emergence of the Covid-19 pandemic (Wave 1, 2018) and compare this to its exact replication, sampling from the same population, that we conducted during the first wave of the pandemic (Wave 2, 2020). Tightness-looseness theory predicts and evidence generally supports that both cooperation and accompanying social norms should increase, yet, we mostly did not find this. Contributions, the probability of reaching the threshold (cooperation), and the contents of the social norm (how much people should contribute) remained similar across the waves, although the strength of these social norms were slightly greater in Wave 2. We also study whether the results from Wave 1 that should not be affected by the pandemic—the relationship between social norms and cooperation and specific behavioural types—replicate in Wave 2 and find that these results generally hold. Overall, our work demonstrates that social norms are important drivers of cooperation, yet, communicable diseases, at least in the short term, have little or no effects on either.

Assessment of stakeholders' perspectives on infill buildings in the historical settings of an eastern India state – Odisha

PurposeThe infill design approach for heritage settings is a challenging task, and it draws the attention of design professionals and residents. The extant literature has advocated for a contextual design approach for new buildings in heritage sites. However, the degree of contextualism for a new building in heritage sites is subjective, and it varies between exact replication and contrast scales. This study aims to evaluate an appropriate design approach for historic precincts of Odisha, an eastern state of India.Design/methodology/approachTwo prime eastern heritage sites (Puri and Ekamra Kshetra) are selected as cases in this study. This research methodology involves identifying key architectural elements from both sites and a questionnaire (prepared by design experts) based on interviewing 400 residents and 36 design professionals on their aesthetic preferences for the different architectural styles and elements. The questionnaire was prepared by the design experts based on the identified architectural styles and elements of both sites. Descriptive statistics and correlation analysis are used to measure the significance of design approaches and elements.FindingsThis study's outcome confirms that most of the respondents (design professionals and residents) prefer the replication design approach with traditional architectural elements of Odisha. Also, documentation of the chronological development of architectural styles and elements of heritage sites of Odisha is done in this research.Research limitations/implicationsThis study has a few limitations: first, the land use characters (mixed, residential, commercial, etc.) of buildings in the heritage precinct are not considered in this research; second, this research has not included the financial aspect of infill design and last, the impact of respondents' socioeconomic factors on their aesthetic perceptions is not considered in this research.Practical implicationsThe development authorities can use the outcomes of this research to implement a design strategy for infill buildings in the historical sites of Odisha.Social implicationsThis research article has documented traditional architectural elements of two prime heritage sites of India.Originality/valueTo date, no quantitative research has been done on infill design approaches in any Indian heritage precincts. This is the first quantitative research on the perception of stakeholders and users on the infill design in historical settings of Eastern India. This research has identified key architectural styles, elements and materials of the heritage sites.

Mixed-integer programming models for irregular strip packing based on vertical slices and feasibility cuts

The irregular strip-packing problem, also known as nesting or marker making, is defined as the automatic computation of a non-overlapping placement of a set of non-convex polygons onto a rectangular strip of fixed width and unbounded length, such that the strip length is minimized. Nesting methods based on heuristics are a mature technology, and currently, the only practical solution to this problem. However, recent performance gains of the Mixed-Integer Programming (MIP) solvers, together with the known limitations of the heuristics methods, have encouraged the exploration of exact optimization models for nesting during the last decade. Despite the research effort, there is room to improve the efficiency of the current family of exact MIP models for nesting. In order to bridge this gap, this work introduces a new family of continuous MIP models based on a novel formulation of the NoFit-Polygon Covering Model (NFP-CM), called NFP-CM based on Vertical Slices (NFP-CM-VS). Our new family of MIP models is based on a new convex decomposition of the feasible space of relative placements between pieces into vertical slices, together with a new family of valid inequalities, symmetry breakings, and variable eliminations derived from the former convex decomposition. Our experiments show that our new NFP-CM-VS models outperform the current state-of-the-art MIP models. Ten instances are solved up to optimality within one hour for the first time, including one with 27 pieces. Finally, we provide a detailed reproducibility protocol and dataset as supplementary material to allow the exact replication of our models, experiments, and results.

Gut microbiota and carcinogenesis: actual aspects

The microbiota, together with the host, form a symbiotic relationship in which the microbiota plays a key role in maintaining the homeostasis of the human body, performing a number of significant functions such as energy metabolism, maturation and maintenance of the immune system, vitamin synthesis, regulation of bile acid reabsorption in the intestine, and much more. Scientific research in recent years has made a significant contribution to understanding the complex relationship between the microbiota and a range of human pathologies, including malignant neoplasms.
 The review considers the mechanisms of the possible influence of bacteria on the development and progression of cancer with an emphasis on the procarcinogenic properties of the microbiota. The most important factor in the mechanism of influence of the microbiota on carcinogenesis are toxins produced by microorganisms that induce direct damage to host cell DNA, causing DNA mutations, disruption of its exact replication, and also provoke an imbalance in the proliferation and apoptosis of host cells, their rapid aging and oncogenesis. The probable mechanisms of participation of microorganisms in the development of cancer through the activation of TLRs and NLRs receptors, which have a tumor-activating effect, are considered. A brief review is given on the mechanisms of carcinogenesis associated with the metabolic activity of the microbiota due to the processes of regulation of the production of secondary bile acids, activation of pro-carcinogenic compounds: phenols, ethanol, sulfides, ammonia, nitrosamines. The influence of the microbiota on the metabolism of sex hormones and the development of hormone-dependent cancers mediated by the mechanisms of enterohepatic circulation and estrogen deconjugation is described.
 The study of the carcinogenic mechanisms of action of the microbiota in the host organism opens up prospects for the development of new successful personalized approaches to the diagnosis, treatment, and prevention of cancer. Changing the composition of the microbiota should become a way to fight cancer, along with surgical treatment, chemotherapy, radiation therapy, targeted therapy and immunotherapy.

Sharing neurophysiology data from the Allen Brain Observatory.

Nullius in verba ('trust no one'), chosen as the motto of the Royal Society in 1660, implies that independently verifiable observations-rather than authoritative claims-are a defining feature of empirical science. As the complexity of modern scientific instrumentation has made exact replications prohibitive, sharing data is now essential for ensuring the trustworthiness of one's findings. While embraced in spirit by many, in practice open data sharing remains the exception in contemporary systems neuroscience. Here, we take stock of the Allen Brain Observatory, an effort to share data and metadata associated with surveys of neuronal activity in the visual system of laboratory mice. Data from these surveys have been used to produce new discoveries, to validate computational algorithms, and as a benchmark for comparison with other data, resulting in over 100 publications and preprints to date. We distill some of the lessons learned about open surveys and data reuse, including remaining barriers to data sharing and what might be done to address these.

The composite effect reveals that human (but not other primate) faces are special to humans.

Recognising faces is widely believed to be achieved using "special" neural and cognitive mechanisms that depend on "holistic" processing, which are not used when recognising other kinds of objects. An important, but largely unaddressed, question is how much like a Human face a stimulus needs to be to engage this "special" mechanism(s). In the current study, we attempted to answer this question in 3 ways. In Experiments 1 and 2 we examined the extent to which the disproportionate inversion effect for human faces extends to the faces of other species (including a range of other primates). Results suggested that the faces of other primates engage the mechanism responsible for the inversion effect approximately as well as that mechanism is engaged by Human faces, but that non-primate faces engage the mechanism less well. And so primate faces, in general, seem to produce a disproportionate inversion effect. In Experiment 3 we examined the extent to which the Composite effect extends to the faces of a range of other primates, and found no compelling evidence of a composite effect for the faces of any other primate. The composite effect was exclusive to Human faces. Because these data differ so dramatically from a previously reported study asking similar questions Taubert (2009), we also (in Experiment 4) ran an exact replication of Taubert's Experiment 2, which reported on both Inversion and Composite effects in a range of species. We were unable to reproduce the pattern of data reported by Taubert. Overall, the results suggest that the disproportionate inversion effect extends to all of the faces of the non-human primates tested, but that the composite effect is exclusive to Human faces.

The competitive advantage of sanctioning institutions revisited: A multilab replication.

Is peer sanctioning a sustainable solution to the problem of human cooperation? We conducted an exact multilab replication (N = 1,008; 7 labs × 12 groups × 12 participants) of an experiment by Gürerk, Irlenbusch, and Rockenbach published in Science in 2006 (Gürerk Ö, Irlenbusch B, Rockenbach B. The competitive advantage of sanctioning institutions. 2006. Science. 312(5770):108-111). In GIR2006 (N = 84; 1 lab × 7 groups × 12 participants), groups that allowed members to reward cooperators and punish defectors were found to outgrow and outperform groups without a peer-sanctioning institution. We find GIR2006 replicated in accordance with all preregistered replication criteria in five of the seven labs we sampled. There, the majority of participants joined groups with a sanctioning institution, and participants cooperated and profited more on average than in groups without a sanctioning institution. In the two other labs, results were weaker but still favored sanctioning institutions. These findings establish the competitive advantage of sanctioning institutions as a robust phenomenon within the European context.

An investigation of mental rotation in infancy using change detection

Two experiments were conducted to examine mental rotation in 6- to 12-month-old infants (N = 166) using a change detection task. These experiments were replications of Lauer and Lourenco (Lauer et al., 2015; Lauer & Lourenco, 2016), using identical stimuli and variations of their procedure, including an exact replication conducted in a laboratory setting (Experiment 1), and an online assessment using Lookit (Scott et al.,2017; Scott & Schulz, 2017) (Experiment 2). Both experiments failed to replicate the results of the original study; in neither experiment did infants’ behavior provide evidence that they mentally rotated the object. Results are discussed in terms of the robustness of mental rotation in infancy and about limits in our experimental procedures for uncovering perceptual and cognitive abilities in infants.

Beyond replication: An exact replication study of Łodzikowski (2021)

AbstractReplication studies have become an emerging line of research in recent decades, including in computer-assisted language learning (CALL). Exact replication, which closely follows a study’s protocol, is rare as it is hard to recreate results without establishing a highly controlled environment. However, using data available online, we were able to conduct an exact replication of Łodzikowski’s (2021) study, which reported on the use of an allophonic transcription tool by 55 Polish learners of English. Allophonic features are used by native speakers to produce acoustic variants of the same phoneme. The original study offered learners an allophonic transcription tool, examined how they used it and considered its association with phonological awareness. This study extended the original research by addressing the limitations of its regression and transcription analyses. Our findings allowed us to offer several suggestions on (1) how an allophonic transcription tool can be better designed to help learners, (2) how CALL researchers can acquire more data for more useful research and (3) why more replication studies are needed in CALL.

Pure Verbal Autopalinacousis

AbstractIntroductionPalinacousis, the phenomenon in which sounds are internally perseverated or repeated has not heretofore been reported occurring exclusively to an individual’s own voice.MethodsA 52-year-old woman started to experience auditory hallucinations of her voice at 3 years old when she began talking out loud. The auditory hallucinations of her own voice, yclept autopalinacousis, consisting of one to three words, were affectively neutral, rarely disruptive, and unchanged by psychiatric medications. During these palinacoustic phenomena, she would hear the last few words she had spoken out loud repeat inside her head in both ears. When the autopalinacousis occurred, the words were repeated just once. Sound quality was an exact replication of how it was originally spoken. She only experienced the palinacousis to her own voice and never to any other sounds. For the palinacousis to occur, she had to verbally state the words loud enough for her to hear. If she spoke out loud but could not hear her own voice, via occlusion of her external auditory canal or presence of loud noise, the internal auditory repetition would not occur. However, after the auditory stimulus was sensed, nothing could reduce the palinacousis. The palinacousis could occur if she read out loud, but not if she read silently. The frequency of the autopalinacousis ranged from a few times a week to several times a day and was associated with reduced sleep, but unaffected by mood, psychiatric medications, or headaches. Five months prior to her psychiatric hospitalization, she began to experience paranoid delusions, decreased sleep, increased activity, rapid speech, and auditory hallucinations of one male and two female voices. In contrast to autopalinacousis, these auditory hallucinations consisted of full phrases or sentences, were affectively charged, intrusive, and diminished by psychiatric medications. No palinacousis occurred with the hallucinated voices.ResultsAbnormalities: Mental Status Examination: Speech: pressured. Oriented x2. Memory: ability to remember 5 digits forwards and 2 digits backwards. She is not able to spell with word “world.” Calculation ability: poor. MRI of brain with and without contrast: normal.DiscussionIn cases where patients with psychotic illness experience palinacousis, the palinacousis always appear after the psychotic illness has already manifested, anywhere from less than a year to 15 years later. Our patient’s palinacousis presented almost 5 decades before the onset of her auditory hallucinations and paranoid delusions. Furthermore, her palinacousis only occurred to her own spoken voice and never to any other voices. In those who present with auditory hallucinations, query as to the presence and characteristics of palinacousis is warranted.FundingNo Funding

Robustness of the cognitive gains in 7-month-old bilingual infants: A close multi-center replication of Kovács and Mehler (2009).

We present an exact replication of Experiment 2 from Kovács and Mehler's 2009 study, which showed that 7-month-old infants who are raised bilingually exhibit a cognitive advantage. In the experiment, a sound cue, following an AAB or ABB pattern, predicted the appearance of a visual stimulus on the screen. The stimulus appeared on one side of the screen for nine trials and then switched to the other side. In the original experiment, both mono- and bilingual infants anticipated where the visual stimulus would appear during pre-switch trials. However, during post-switch trials, only bilingual children anticipated that the stimulus would appear on the other side of the screen. The authors took this as evidence of a cognitive advantage. Using the exact same materials in combination with novel analysis techniques (Bayesian analyses, mixed effects modeling and cluster based permutation analyses), we assessed the robustness of these findings in four babylabs (N=98). Our results did not replicate the original findings: although anticipatory looks increased slightly during post-switch trials for both groups, bilingual infants were not better switchers than monolingual infants. After the original experiment, we presented additional trials to examine whether infants associated sound patterns with cued locations, for which we did not find any evidence either. The results highlight the importance of multicenter replications and more fine-grained statistical analyses to better understand child development. HIGHLIGHTS: We carried out an exact replication across four baby labs of the high-impact study by Kovács and Mehler (2009). We did not replicate the findings of the original study, calling into question the robustness of the claim that bilingual infants have enhanced cognitive abilities. After the original experiment, we presented additional trials to examine whether infants correctly associated sound patterns with cued locations, for which we did not find any evidence. The use of novel analysis techniques (Bayesian analyses, mixed effects modeling and cluster based permutation analyses) allowed us to draw better-informed conclusions.

Does stress consistently favor habits over goal-directed behaviors? Data from two preregistered exact replication studies

Instrumental learning is controlled by two distinct parallel systems: goal-directed (action-outcome) and habitual (stimulus-response) processes. Seminal research by Schwabe and Wolf (2009, 2010) has demonstrated that stress renders behavior more habitual by decreasing goal-directed control. More recent studies yielded equivocal evidence for a stress-induced shift towards habitual responding, yet these studies used different paradigms to evaluate instrumental learning or used different stressors. Here, we performed exact replications of the original studies by exposing participants to an acute stressor either before (cf. Schwabe and Wolf, 2009) or directly after (cf. Schwabe and Wolf, 2010) an instrumental learning phase in which they had learned that distinct actions led to distinct, rewarding food outcomes (i.e., instrumental learning). Then, following an outcome devaluation phase in which one of the food outcomes was consumed until participants were satiated, action-outcome associations were tested in extinction. Despite successful instrumental learning and outcome devaluation and increased subjective and physiological stress levels following stress exposure, the stress and no-stress groups in both replication studies responded indifferently to valued and devalued outcomes. That is, non-stressed participants failed to demonstrate goal-directed behavioral control, thereby rendering the critical test of a shift from goal-directed to habitual control in the stress group inapt. Several reasons for these replication failures are discussed, including the rather indiscriminate devaluation of outcomes that may have contributed to indifferent responding during extinction, which emphasize the need to further our understanding of the boundary conditions in research aimed at demonstrating a stress-induced shift towards habitual control.

Advances and Challenges in Spatial Transcriptomics for Developmental Biology.

Development from single cells to multicellular tissues and organs involves more than just the exact replication of cells, which is known as differentiation. The primary focus of research into the mechanism of differentiation has been differences in gene expression profiles between individual cells. However, it has predominantly been conducted at low throughput and bulk levels, challenging the efforts to understand molecular mechanisms of differentiation during the developmental process in animals and humans. During the last decades, rapid methodological advancements in genomics facilitated the ability to study developmental processes at a genome-wide level and finer resolution. Particularly, sequencing transcriptomes at single-cell resolution, enabled by single-cell RNA-sequencing (scRNA-seq), was a breath-taking innovation, allowing scientists to gain a better understanding of differentiation and cell lineage during the developmental process. However, single-cell isolation during scRNA-seq results in the loss of the spatial information of individual cells and consequently limits our understanding of the specific functions of the cells performed by different spatial regions of tissues or organs. This greatly encourages the emergence of the spatial transcriptomic discipline and tools. Here, we summarize the recent application of scRNA-seq and spatial transcriptomic tools for developmental biology. We also discuss the limitations of current spatial transcriptomic tools and approaches, as well as possible solutions and future prospects.