Ex Situ Research Articles

Genetic diversity and structure of Hopea bilitonensis, an endemic Dipterocarp from Belitung Island, Indonesia

Hopea bilitonensis Ashton (Dipterocarpaceae) is a small tree endemic to some islands on the east coast of Sumatra, particularly Belitung Island, Indonesia, and the northwest of Malaysia. Forest fragmentation and degradation in Belitung Island could affect population and genetic diversity of this species. The main objectives of this study are to examine genetic diversity and structure of H. bilitonensis on Belitung Island, Sumatra. Multiplexed ISSR genotyping by sequencing (MIG-seq) is used for genotyping and SNP detection. Genetic diversity and structure were determined using STRUCTURE analysis. We found that genetic diversity of H. bilitonensis is lower in seedlings than in juvenile and mature individuals. In contrast, inbreeding coefficient is lower in mature individuals than in the seedlings and juveniles. Genetic clusters (K=3) are detected in the early regeneration stages. These genetic structure separates the population of seedling and juvenile stages more clearly than in the mature individuals. Representation of these genetic clusters is essential when collecting propagation material for ex-situ conservation and establishing new populations of this threatened species to ensure its sustainability.

Ethnobotanical study of wild edible plants in Dibatie district, Metekel zone, Benishangul Gumuz Regional State, western Ethiopia

BackgroundPlants deliver livelihood and food for millions of people in the world. Indeed, wild edible plants support rural communities in developing countries to overcome seasonal unfavorable conditions. In rural areas of Ethiopia, wild edible plants play an indispensable role in fighting food insecurity as emergency or supplementary foods. Hence, this research was aimed at studying the ethnobotanical assessment of wild edible plants in Dibatie district, Metekel zone, western Ethiopia.MethodsEthnobotanical data was collected using a semi-structured interview, field observation, focus group discussions, a market survey, and the ranking of selected plants. Besides, voucher specimens were collected and stored at the National Herbarium of Ethiopia. Descriptive statistics, preference ranking, direct matrix ranking, and familiarity index were computed for data analysis.ResultsThis study has documented 54 wild edible plant species belonging to 33 plant families and 46 genera. Of these, most (38.90%) had tree growth habits. Wild edible plants bear mostly fruits (72.20%) as edible parts. Local people usually consume these plants freshly raw as complementary foods, though some wild edibles require processing. They were mostly harvested in the January (31.48%) and May (27.78%) months, with the least collected in September (7.41%). Most wild edible plants (78.57%) were available in uncontrolled habitats, while others (21.43%) live in farmlands, home gardens, and as live fences. Out of the recorded plants, about 98% had additional uses besides their nutritional values.ConclusionWild edible plants assist the livelihoods of the local people in food security, agriculture, energy sources, construction, medicines, ecological services, aesthetics, income generation, and household utensils. Nevertheless, wild edible plants are recently threatened due to various anthropogenic factors in the study area. Thus, they need wise use and in-situ and ex-situ conservation measures from all the concerned bodies for sustainable use in the future.

Markers of fertility in reproductive microbiomes of male and female endangered black-footed ferrets (Mustela nigripes)

Reproductive microbiomes contribute to reproductive health and success in humans. Yet data on reproductive microbiomes, and links to fertility, are absent for most animal species. Characterizing these links is pertinent to endangered species, such as black-footed ferrets (Mustela nigripes), whose populations show reproductive dysfunction and rely on ex-situ conservation husbandry. To understand microbial contributions to animal reproductive success, we used 16S rRNA amplicon sequencing to characterize male (prepuce) and female (vaginal) microbiomes of 59 black-footed ferrets at two ex-situ facilities and in the wild. We analyzed variation in microbiome structure according to markers of fertility such as numbers of viable and non-viable offspring (females) and sperm concentration (males). Ferret vaginal microbiomes showed lower inter-individual variation compared to prepuce microbiomes. In both sexes, wild ferrets harbored potential soil bacteria, perhaps reflecting their fossorial behavior and exposure to natural soil microbiomes. Vaginal microbiomes of ex-situ females that produced non-viable litters had greater phylogenetic diversity and distinct composition compared to other females. In males, sperm concentration correlated with varying abundances of bacterial taxa (e.g., Lactobacillus), mirroring results in humans and highlighting intriguing dynamics. Characterizing reproductive microbiomes across host species is foundational for understanding microbial biomarkers of reproductive success and for augmenting conservation husbandry.

Angiosperm flora of Federal University Oye-Ekiti, Oye Campus – A preliminary checklist

This study investigated and documented the diversity of angiosperms found within the main campus of Federal University, Oye-Ekiti, Nigeria. Given the numerous and continuous threat to species availability through infrastructural development, the study was aimed at preparing a checklist of all plant species existing within the campus prior to complete development of the University area. Species enumeration was carried out using conventional methods as described by authors of related studies. A total of 150 species distributed in 47 families and 112 genera was recorded, comprising herbs, creepers, climbers, geophytes, lianas, shrubs and trees. The shrubs dominated the list, with a total of fifty-five species (36.7%). The families Euphorbiaceae and Asteraceae were the most represented with 10.7% and 9.3% occurrence, followed by Fabaceae (Papilionoideae) with 7.3% (11 species). Twenty-one (21) families were represented with 1 species each, an indication of their importance in ecosystem balancing and habitat restoration. With the current pace of infrastructural development within the University Campus, we suggest the immediate establishment of arboretum or botanical garden for ex-situ conservation of the plant genetic resources within the study area.

Visitor Perception on Urban Green Space and its Contribution for Human Well-being, A Case Study for Henarathgoda Botanic Garden


 
 
 Botanical gardens vary widely in design and purpose, but most are typically associated with Ex-situ conservation, education, or historical interpretation. Yet, few studies have shown that botanic garden visitors are often motivated by recreational and leisure interests. While visitor motivations and benefits have been the focus of a number of botanical garden and green space visitor studies. The main objective of the study was to investigate the perceptions of benefits for wellbeing of community, by visiting Henarathgoda Botanic Garden (HBG) and the Specific objectives were; to identify the range of different specialized visitors to the HBG, identify the activity patterns of community visitation associated with HBG and to examine visitor preferences/perceptions for various combinations of social, resource and management conditions, and study the different wellbeing benefits obtained by visiting the garden related to physical and aesthetics aspects. This study employed means-end theory to investigate the links visitors make between garden attributes, consequences, and values. Structured questionnaire based in-person interviews were conducted with garden visitors (n=200) and surveys took place representing whatsoever the community visiting the HBG during February to March 2022. The overall study resulted that the garden visitation has been drastically affected by COVID-19 pandemic situation before, during, and after the pandemic respectively. This study reveals that the main top two reasons for visiting the HBG of respondent were because of relaxation and pleasure. This shows HBG should provide a suitable place for recreational activities for visitors. Further, the highest percentage of the respondents (36%) were commented that HBG‘s management should consider improving the services providing and maintaining more facilities and need for the visitors. The most positive benefit wanted to gain responders by visiting HBG, which was understanding and enjoying nature in different garden sections established at HBG (42%).
 Keywords: Urban green spaces, Well-being benefits, Botanic garden, Psychological benefits, Questionnaire survey
 
 

Preferensi tenggeran burung di Pulau Jawa sebagai rujukan untuk konservasi ex-situ: Kajian pustaka

The rate of growth of society in Indonesia, especially on the island of Java, has meant that much land has been converted into residential areas. This causes several bird species to lose their habitat and become endangered, so conservation is needed as an effort to preserve them. This research aims to determine the perching preferences of birds on the island of Java, making it easier to create a living environment that suits their natural environment. This research uses qualitative methods through a literature review of literature sources, articles, and scientific journals. The IUCN Red List page is used to determine the scope of the aves species discussed in this article, namely the island of Java, and to find out the aves' habitat so that their perch preferences can be found. Three different categories of perches were obtained, namely texture, height, and perch diameter, with the assumption that these data could be translated to create artificial perches for ex-situ conservation.

Effect of Benzyl Amino Purine (BAP) on the Leaf Growth of Vanda limbata Blume Orchid In Vivo

<p><em>Vanda limbata</em> is a natural orchid with slow growth and is often taken directly from the forest in large quantities, threatening its sustainability. Ex-situ preservation efforts are needed for this orchid, one of which is through cultivation with the addition of Benzyl Amino Purin (BAP), known to increase shoot growth. This research aims to analyze the effect of BAP and determine its optimal concentration on the growth of <em>V. limbata</em> orchids. This research used a single-factor completely randomized design in BAP concentrations of 0, 25 and 50 mg l<sup>-1</sup> with 5 replications. The method used was spraying BAP solution once a week on all parts of adult <em>V. limbata</em> orchids aged ±2 years. The research was conducted for 3 months in the experimental garden with parameters observed in the form of the number of new leaves, elongation of old leaves, the length of new leaves, increase in the width of old leaves, the width of new leaves, stomatal density and the number of roots. The results showed that the most effective BAP concentration was 50 mg l<sup>-1</sup> for the number of new leaves which increased by 200%, the length of old leaves which increased by 168.66% and the length of new leaves which increased by 800%, but resulted in less stomatal density than the control. BAP can increase the growth of <em>V. limbata</em> orchid leaves. This research can be a recommendation for ex-situ conservation efforts that have the potential to be developed as a means of orchid cultivation.</p>

Vernalization affects the germination performance of the wetland endangered species Eleocharis carniolica

AbstractEleocharis carniolica W.D.J. Koch (Cyperaceae) is an endangered wetland spike rush mainly threatened by habitat loss and fragmentation. Understanding the germination ecology of this species is essential to perform successful conservation and restoration actions. In this study, we investigated the effect of vernalization (i.e. cold stratification), gibberellic acid (GA3) and chemical scarification on seed germination of E. carniolica from wild populations in northern Italy. The results showed that vernalization (i.e. 8-weeks at 4 °C) significantly improved germination probability, speed, and uniformity compared to non-stratified seeds. Gibberellic acid treatment alone or in combination with vernalization did not show a significant improvement in germination. Chemical scarification using sodium hypochlorite increased germination probability, with 8 h of scarification showing the highest success rate. However, 24-h scarification had a negative impact on germination. Overall, vernalization was found to be the most effective method to enhance germination in E. carniolica. These findings provide valuable insights into the seed germination ecology of this endangered species, aiding in its exsitu conservation, propagation, and in-situ restoration efforts. Moreover, they have important implications on future germination dynamics of this endangered species, especially with predicted climate change scenarios.

Near extinct Argyreia versicolor and rare Argyreia mekongensis are dependent on carpenter bee pollinators.

Argyreia versicolor and Argyreia mekongensis are extremely rare plant species. The former had not been seen for nearly 100 years until two individuals were found in Thailand in 2018, and only a handful of populations are known for the latter. The aims of this study were to examine the breeding systems of A. versicolor and A. mekongensis using pollination experiments and to determine their potential pollinators via floral observations. Our controlled pollination experiments uncovered the self-incompatibility of both species. Pollinator censuses indicated that females of two carpenter bee species, Xylocopa aestuans and Xylocopa latipes, were the predominant floral visitors for both Argyreia species. Our observations confirmed a harmonious match between the floral shape of both Argyreia species and the body sizes of these pollinators, ensuring effective pollen transfer and validating their role as putative pollinators. In line with the high frequency of pollinator visits observed, our controlled pollination experiments found no evidence of pollen limitation under field conditions. The findings of this study hold significance for the conservation of these endangered species, yet the situation is dire for A. versicolor, with one of the two individuals under study recently lost. Hence, it is crucial to intensify monitoring efforts for the species, aiming to identify additional individuals for potential inclusion in an ex-situ conservation program. Simultaneously, safeguarding the habitat of these plant species and their pollinators will be critical.

A decision framework to integrate in-situ and ex-situ management for species in the European Union

Zoos and aquaria in the European Union (EU) can play a crucial role in the conservation of EU species, as they currently hold nearly half (49%) of EU terrestrial vertebrates. In this study, we analyzed the species composition and population sizes of EU zoos and developed a framework to prioritize recommendations for additional ex-situ and in-situ interventions for 277 at-risk EU species. Our results showed that EU zoos currently hold 39% of threatened EU species, 27% of EU endemic species, 62% of EU species vulnerable to climate change, 20% of EU species listed by the Alliance for Zero Extinction (AZE), 25% of Evolutionary Distinct and Globally Endangered (EDGE) EU species, while only 5% are subject to ex-situ conservation. Using our framework, we found that additional captive breeding was recommended for 60-61%% of species while expanding protected areas was recommended for only 2–22%, as 217 out of 277 species already met habitat protection targets. Both interventions were recommended for up to 20% of species, while the remaining 18% required no interventions because captive populations and habitat protection fully met targets. Our flexible framework can support more effective integrated conservation planning decisions for EU species and help identify target species for further in-depth assessment by the IUCN Ex-situ guidelines.

Population status, habitat preferences and predictive current and future distributions of three endangered Silene species under changing climate.

One of the most crucial steps in the practical conservation of endangered endemic mountain plants is to address their population size status and habitat requirements concurrently with understanding their response to future global warming. Three endangered Silene species-Silene leucophylla Boiss., S. schimperiana Boiss., and S. oreosinaica Chowdhuri-in Egypt were the focus of the current study. These species were examined for population status change, habitat quality variables (topography, soil features, and threats), and predictive current and future distributions. To find population size changes, recent field surveys and historical records were compared. Using Random Forest (RF) and Canonical Correspondence Analysis (CCA), habitat preferences were assessed. To forecast present-day distribution and climate change response, an ensemble model was used. The results reported a continuous decline in the population size of the three species. Both RF and CCA addressed that elevation, soil texture (silt, sand, and clay fractions), soil moisture, habitat-type, chlorides, electric conductivity, and slope were among the important variables associated with habitat quality. The central northern sector of the Saint Catherine area is the hotspot location for the predictive current distribution of three species with suitable areas of 291.40, 293.10, and 58.29 km2 for S. leucophylla, S. schimperiana, and S. oreosinaica, respectively. Precipitation-related variables and elevation were the key predictors for the current distribution of three Silene species. In response to climate change scenarios, the three Silene species exhibited a gradual contraction in the predictive suitable areas with upward shifts by 2050 and 2070. The protection of these species and reintroduction to the predicted current and future climatically suitable areas are urgent priorities. Ex-situ conservation and raised surveillance, as well as fenced enclosures may catapult as promising and effective approaches to conserving such threatened species.

Grasses of Acharya Jagadish Chandra Bose Indian Botanic Garden (AJCBIBG), Howrah, India

Grasses being the members of the family Poaceae deserves special attention as it has an integral role in the lives of both human beings and animals. From ecological point of view their role is immense but at the same time grasses are neglected since time immemorial due to difficulties to work out scientifically. Realizing the very fact, the present study aimed at listing out the grasses growing in the Acharya Jagadish Chandra Bose Indian Botanic Garden (AJCBIBG), an old and historic garden as well as, an excellent centre for ex-situ conservation of plant wealth from across the globe. This communication deals with enumeration of the grasses (32 species) dwelled in the garden premises along with correct nomenclature and synonyms available if any. Concurrently, flowering and fruiting time, and notes are also provided wherever essential including utilitarian values.

Sperm morphology, and macro and microscopic description of male and female reproductive tract in the North African houbara bustard (Chlamydotis undulata)

Ex-situ conservation programs are useful tools for preserving endangered species, and knowledge of their reproductive physiology is essential for achieving conservation objectives in captive breeding programs. The North African houbara bustard (Chlamydotis undulata) is a vulnerable bird species bred in captivity to restore its natural populations. To better understand the reproductive biology of this species, we first described the anatomy and histology of the reproductive tracts of male and female North African houbara bustards. We described and classified the normal and abnormal sperm morphologies for the species. The reproductive tracts of males and females were collected during the breeding season (BS) (21 males and 14 females) and the non-breeding season (NBS) (four males and two females). Pictures and measurements of the testes and oviducts were taken, and testis colour was graded from 1 (black) to 6 (white). Organs were fixed and processed for histological analysis, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The testis, epididymis, oviduct size, testicular capsule thickness, seminiferous tubule (ST) diameter, and histological layers of the oviduct were compared between the BS and NBS groups. Sperm morphometry was evaluated using fixed ejaculates (n=22, from 22 males). Ejaculates (n=417, 166 males) were collected and fixed during the BS, and their morphology and morphometry were evaluated using phase-contrast microscopy. Ejaculate mixes (n=12) were fixed and analysed using SEM and TEM. Overall, the anatomical structure and histology of the male and female reproductive tracts were similar to those of other avian species. The testes were symmetric, and a regression of testis and oviduct size, the thickness of the mucosa and serosa of the oviduct, and ST diameter (p<0.001) were observed during NBS compared with those during BS. Sperm storage structures are located inside the oviduct at the uterovaginal junction and infundibulum. Melanin granules were observed in the interstitial space of the seminiferous epithelium, which explains the testis pigmentation. Melanin was also found in the ovaries and the uterus. Houbara sperm have an elongated nuclear form and a round-shaped acrosome lacking perforatorium and rostrum. Most analysed samples were limitrophe normospermic (normal= 68.5 ± 14.7%), houbara sperm morphology could be classified into eight categories: normal sperm, acrosome, head, midpiece, tail, and teratogenic defects, swollen head, and cytoplasmic droplet. This study provides the first anatomical and histological descriptions of the male and female reproductive tracts of the North African houbara bustard. Further investigation into the functional role of melanin in the testes is warranted.

Ex-situ Conservation of Palms with Special Reference to Endemic and IUCN Red List Species in Acharya Jagadish Chandra Bose Indian Botanic Garden, Howrah, India

Acharya Jagadish Chandra Bose Indian Botanical Garden (AJCBIBG) also known as Company Bagan, or Royal Botanic Garden and is one of the best landscaped gardens of the World. The garden serves as a hub for ex-situ conservation of plant species including native, endemic and threatened plants. The present paper deals with the holistic approach of palms diversity, endemism, conservation status, economic importance of different species of palms in AJCBIBG are discussed. AJCBIBG has 110 species of palms including 11 endemic and 46 IUCN Red listed species. Thus, it provides immense opportunities for research, education, also spread awareness about conservation, multiplication and utilization of palms to the society.

Analysis of Medicinal Plants and Traditional Knowledge Development in Ethiopia

As the review conducted Ethiopia has richened by medicinal plant species and traditional knowledge that have a significant role in the management of various human and livestock diseases. Medicinal plants are vastly used by the communities particularly rural communities to treat various human and livestock aliments.Medicinal plants also important for the development of modern medicine. Traditional knowledge is the accumulation of communities’ knowledge according to their cultures, beliefs, and experiences. Different parts of medicinal plants are used for traditional remedies in order to cure human beings and livestock. Traditional knowledge and medicinal plants are critically threatened and extinct due to overgrazing, charcoal production, deforestation, urbanization, agricultural expansion, and fire wood collection. Traditional medicinal plants and associated knowledge can be conserved using either in-situ or ex-situ conservation methods. Therefore, create awareness among the local people about the use of medicinal plants and the associated knowledge to ensure sustainable harvesting of medicinal plants. Conducting ethnobotanical research and documented medicinal plants and indigenous knowledges is very important for conserve.

Respon Eksplan Buku Durian Lahung (Durio dulcis) terhadap Konsentrasi BAP (Benzil Amino Purin) pada Media WPM (Woody Plant Medium)

Lahung is a genus of endemic Durio in Kalimantan which currently has vulnerable status and is rare. Therefore, ex-situ conservation of this plant has begun using tissue culture techniques. Several things that need to be considered in in vitro propagation are the right type of explant, planting mediun and PGR. Stem nodes are generally chosen as explant because their availability does not depend on the season and has a fast response in bud induction. WPM medium is e medium intended for woody plants such as durian lahung. BAP is a PGR from the cytokinin group which is quite avtive and the most widely used. This research aimed to determine the best response of the durian lahung stem node culture to the BAP concentration on WPM medium. This research was designed using a rancomized block design (RBD) with one factor in the form of BAP concentration consisting of six levels, i.e. 0.0, 0.5, 1.0, 1.5, 2.0 and 2.5 ppm. Each experimental unit was repeated four times and consisted of ten planting bottles for e total of 240 planting bottles. The variables observed were the percentage of live explant aged 1-8 WPP, callus growth time, percentage of explant forming callus aged 1-8 WAP, callus and texture callus, shoot growth time and number of shoots. The results showed that callus appeared in response of the durian lahung stem node to the BAP concentration treatments.

In vitro asymbiotic propagation of the vulnerable slipper orchid Cypripedium cordigerum D. Don

This paper presents an attempt to establish a protocol for the conservation of Cypripedium cordigerum D. Don a vulnerable species of orchid using in vitro symbiotic seed germination. The suitability of four orchids seed germination media [Terrestrial orchid medium (BM), modified terrestrial orchid seed germination medium (BM-1), Malmgren modified terrestrial orchid medium (MM), Knudson C medium (KC)] was tested using different photoperiods i.e. 24-h dark or 12-h light per day. The seed capsules were harvested at two different stages of development in order to determine the effect of capsule maturity on seed germination. The maximum percentage germination of seed from intact capsules in the dark was 65.00 ± 0.12% on the BM-1 medium. Release of brownish exudates was stopped by the addition of activated charcoal to the cultures. Seedlings developed in 26.17 ± 0.17 weeks. The current study is the first to report the ex-situ conservation of C. cordigerum.

Understanding the phenotypic variability of the olive ‘Galega vulgar’ fruits and oil-related traits across environments

‘Galega vulgar’, a genetically diverse variety, is the main olive tree variety across Portugal, being broadly cultivated across distinct environments. ‘Galega vulgar’ is presently being replaced by higher-yielding, although lower-quality, foreign varieties and efforts must be made to counteract this tendency, and to more efficient explore ‘Galega vulgar’ to the profit of olive tree producers, and the remaining market stakeholders. For that, in the present research, an evaluation of the influence of genetic and environmental factors and their interactions in the phenotypic variability of ‘Galega vulgar’ was performed, focused mainly on important traits to olive stakeholders (namely, fruit-related traits such as fruit and flesh weight and flesh-to-stone ratios and oil-related traits), towards the selection of the best representatives of the variability found. A three-year in-situ detailed agronomic characterization was conducted taking advantage of a previously defined ‘Galega vulgar’ collection composed of 595 trees from 95 clones, cultivated across all the Portuguese geographical districts where ‘Galega vulgar’ has representativeness (10 districts), considering thirteen phenotypic traits.The environment was the main contributor to the phenotypic variability observed on the ‘Galega vulgar’ variety. Significant differences for almost all the analysed traits were detected across years, trees, and geographical districts, with significant district-by-clone interactions. Of the two most frequent clones, clone C002 was the most stable across the geographical districts under study. The present work provides information on the phenotypic variability of ‘Galega vulgar’ important fruit and oil related traits across different environments, of utmost importance from the conservation, breeding, resilience, and production perspectives, considering the pressing need to adapt to climate change effects. In addition, since a significant geographic district x clone interaction was observed, the generated knowledge may now contribute to a better adjustment of the right clone for the right region in what respects new orchards establishment.A set of ‘Galega vulgar’ trees, primarily identified as belonging to the same clones, was selected due to their ‘residual’ phenotypic variability. These trees will contribute to broadening the genetic basis of a previously defined ex-situ conservation base ‘Galega vulgar’ collection of trees, to be trialled in a comparative field experiment, under the same environmental conditions. These experiments are needed for a more precise evaluation of the genetic potential of this variety, towards future genetic studies to clarify the complex genetic control of traditional olive breeding targeted traits and to develop molecular selection tools to support precision olive breeding.

The feeding behavior and food preferences of siamang (Symphalangus syndactylus Raffles, 1821) at Taman Safari Indonesia Bogor

Siamang (Symphalangus syndactylus Raffles, 1821) is a diurnal primate that belongs to the endangered category, according to the IUCN. Siamangs are classified as arboreal and frugivorous animals that selectively choose food. Siamang’s food preferences tend to be determined by the fruit availability in its habitat. One of the efforts to protect siamangs is ex-situ conservation. Feeding in ex-situ conservation should be adjusted to provide in their natural habitat. This study aims to identify siamang’s feeding behavior and food preferences in one of the ex-situ conservation facilities, namely Taman Safari Indonesia Bogor. The objects of observation consisted of two adult males, one adult female, and one sub-adult female. The methods used were instantaneous sampling, focal animal sampling, and ad-libitum sampling—statistical analysis using One-Way ANOVA and Two-Way ANOVA with Tukey’s test. The frequency and duration of siamang feeding behavior differed significantly between age classes, while no difference was based on sex. Food preferences among individuals are influenced by gender. Males prefer fruits such as apples and salak over vegetables, while females prefer soft-stemmed vegetables, namely water spinach and spinach.

Species composition of terrestrial gastropods in an urban conservation area: Cibinong Botanical Garden

A study conducted in the Cibinong Botanical Garden (CBG) aimed to reveal the diversity of terrestrial gastropods. The CBG is an ex-situ conservation area for plants and has become the green space in urban ecosystems. The area is located near the city centre and only a few kilometers away from the cement industry. Research of land snails in CBG was carried out in January 2020 and March 2023. Five plots of 100 m2 were observed using the time search method. In addition, 3L of soil and leaf litter per plot were sorted and sieved to collect micro snails. In a total of 131 specimens from four families were collected. We found a land snail species endemic to Java, Landouria sp. and invasive alien species, Allopeas gracile, Allopeas clavulinum, and Lissachatina fulica. Based on iNEXT analysis, the number of species found in CBG had reached the maximum limit (SC=1). Shannon index value was 1.76 which implies low diversity. Further research is needed to understand environmental factors impacting land snail diversity in CBG. Regardless of the presence of invasive alien species, the CBG is a suitable habitat for land snails and plays an important role as a conservation oasis in urban ecosystems.