Ewes In Late Pregnancy Research Articles

Validation of targeted selective treatment (TST) methodology for gastrointestinal parasites of adult sheep in different physiological states

We aimed to validate a targeted selective treatment (TST) methodology for treating parasitic gastrointestinal infections in ewes in different physiological states using parasitological and hematological parameters. Forty ewes were monitored from December 2021 to June 2022 and evaluated during various physiological stages in their life cycle. Before starting the experiment, a fecal egg count (FEC) reduction test was performed to evaluate the efficacy of the anthelmintic (AH) treatment. Weekly assessments were performed based on the Famacha© (F) system and body condition score (BCS), and ewes were subjected to AH treatment when necessary, with their physiological states recorded. Ewes were treated when they presented F ≥ 3, BCS ≤ 2.0 (when F = 2), or submandibular edema. Parasitological, i.e., FEC, and hematological, i.e., hematocrit (Ht), parameters were evaluated monthly to determine the efficiency of the TST methodology. Comparisons between the mean Ht and FEC values in ewes subjected to AH treatment and untreated ewes were performed using analysis of variance, followed by Tukey’s test. Spearman’s correlation was performed to determine the correlation between the variables, i.e., F scores, BCS, Ht, and FEC. All tests were performed at a significance level of 5 %. During the experimental period, 1138 evaluations were performed. The main reason for AH treatment was F ≥ 3. Ewes in early pregnancy, lactation and late pregnancy received comparatively more AH treatments than the other physiological states. Ewes in late pregnancy and lactation exhibited lower mean Ht values (23.5 % and 22.9 %) and higher mean FEC values (3269 and 1426) compared with those in early pregnancy (30.2 % and 727 EPG). In addition, a statistically significant difference was observed in the Ht and FEC values of ewes that presented submandibular edema (P < 0.001) compared with those that did not exhibit submandibular edema. The genus Haemonchus sp. showed a 96.4 % prevalence in coprocultures. A positive correlation existed between F scores and FEC (r = 0.3819) and a negative correlation between F scores and Ht (r = −0.4728). Ewes that needed AH treatment had lower mean Ht values than ewes that did not need the treatment (19.2 % × 29.3 %; P < 0.001) and higher mean FEC values (8747 × 1163; P < 0.001), confirming that these ewes needed AH treatment. The TST methodology based on F scores, BCS, and submandibular edema could effectively identify individuals in the herd needing AH treatment, identifying 13 % additional cases requiring treatment than using only the F score criterion.

When to shear dairy ewes: before breeding, during pregnancy or let them unshorn?

Shearing dairy ewes may improve their fitness and heat tolerance during late pregnancy, with positive effects on lambs and lactation performances. To test this hypothesis, two Mediterranean breeds (MN, Manchega, n = 43; LC, Lacaune, n = 28), differing in lactation performances and fleece traits, were submitted to three shearing strategies: (i) shorn before breeding (SBB), (ii) shorn at day 100 of pregnancy (S100) and (iii) unshorn (CO). Ewes were bred in spring and gestated during summer. Fleece traits and respiratory rate were measured on pregnant (107–121 days) resting ewes at different barn temperatures. Blood and colostrum were sampled at lambing. Ewes suckled their lambs (28 days) and were machine-milked until 180 days of lactation. Lamb and ewe weights, and condition score of the ewes, were recorded throughout the experiment. Milk yield was assessed during suckling (fortnightly) and milking (daily), and milk was sampled for composition (fortnightly). Fleece extension and wool weight at S100 were 13 and 45% greater in MN than in LC ewes, respectively, but the ewe’s respiration rate at late pregnancy, between 20 and 25 °C, did not vary among shearing treatments nor breeds. Nevertheless, S100 ewes had a 37% lower respiration rate than SBB and CO ewes at 28 °C. At lambing, SBB and S100 ewes had 86% higher glycaemia than CO in MN, but LC ewes did not vary. Shearing treatment had no effect in plasma insulin, β-hydroxybutyrate or non-esterified fatty acids (NEFAs) at lambing in both breeds. Lamb’s birthweight and growth during suckling did not differ by shearing treatment in both breeds. Colostrum composition and milk yield during suckling were not modified by shearing treatment in either breed, although S100 suckling milk composition increased in MN (protein and casein, 6%) and LC ewes (total solids, 8%; fat, 18%), compared to CO and SBB. No effects were detected in milk yield or composition during milking, but S100 tended to yield 28% more milk than CO in the LC ewes. The S100 treatment improved the body reserves in late pregnancy, when compared to CO and SBB in both breeds. In conclusion, shearing dairy ewes in late pregnancy was a recommendable management practice under summer conditions, because alleviated heat stress and improved the weight of the ewes, without detrimental effects on lambing and lactational performances, the last tending to increase milk yield in high-yielding dairy ewes.

Changes in Sheep Behavior before Lambing

The aim of this study was to assess the duration and frequency of behavioral observations of pregnant ewes as they approached lambing. An understanding of behavioral changes before birth may provide opportunities for enhanced visual monitoring at this critical stage in the animal’s life. Behavioral observations for 17 ewes in late pregnancy were recorded during two separate time periods, which were 4 to 6 weeks before lambing and before giving birth. It was normal farm procedure for the sheep to come indoors for 6 weeks of close monitoring before lambing. The behaviors of standing, lying, walking, shuffling and contraction behaviors were recorded for each animal during both time periods. Over both time periods, the ewes spent a large proportion of their time either lying (0.40) or standing (0.42), with a higher frequency of standing (0.40) and shuffling (0.28) bouts than other behaviors. In the time period before giving birth, the frequency of lying and contraction bouts increased and the standing and walking bouts decreased, with a higher frequency of walking bouts in ewes that had an assisted lambing. The monitoring of behavioral patterns, such as lying and contractions, could be used as an alert to the progress of parturition.

Advances in managing pregnancy toxaemia in sheep

Background: Ovine pregnancy toxaemia (OPT) is a metabolic disorder of ewes in late pregnancy. Cases are typically seen in ewes carrying multiple fetuses, hence the alternative name of ‘twin lamb disease’. The disease has been well known for many years. Despite this, the toll inflicted by the disease remains considerable, due both to the poor prognosis of clinically affected animals, even with appropriate treatment, and the impact of the provoking factors of under‐nutrition and concomitant disease on ewe morbidity, lamb survival and flock productivity. Cases of OPT are an indication of shortcomings in the management of late‐pregnancy ewes.Aim of the article: This article provides an update on the aetiopathogenesis, diagnosis, treatment and prevention of OPT, drawing on recently published studies.

Impact of supplementing feed restricted ewes’ diet with propylene glycol, monensin sodium and rumen-protected choline chloride during late pregnancy on blood biochemical indices, body condition score and body weight

The purpose of this study was to investigate the effects of supplementing feed restricted diet with propylene glycol, monensin sodium and rumen-protected choline chloride on blood metabolic status, body condition score (BCS) and body weight of ewes in late pregnancy. At the final six weeks of pregnancy, 48 pregnant Ghezel ewes (body weight 65.53 ± 6.90 kg) were randomly allocated into six groups (n = 8) as follows: control (CG, ad libitum); feed restriction (FR, 60 % of the dry matter intake offered to CG group); feed restriction + propylene glycol (67 g/d) (FR-PG); feed restriction + propylene glycol + monensin sodium (30 mg/d) (FR-MS); feed restriction + propylene glycol + rumen-protected choline chloride (6 g/d) (FR-RPC); feed restriction + propylene glycol + monensin sodium + rumen-protected choline chloride (FR-PMC). Blood samples were collected on days 90, 117, 124, 135 and 140 of pregnancy, at lambing and days 7 and 17 after lambing for analysis of glucose, triglyceride, total cholesterol, total protein, albumin, urea, non-esterified fatty acids (NEFA), ß-hydroxybutyrate (BHBA), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and insulin using the automated biochemical analyzer and ELISA microplate reader. Body weight and BCS of ewes were recorded on days 90, 110, 130 and 140 of pregnancy, at lambing and also day 7 after lambing. All statistical analyses were carried out in a completely randomized design using the MIXED procedure of SAS v.9.3. Results of analysis revealed that pre-parturient administration of rumen-protected choline chloride combined with propylene glycol and monensin sodium elevated both pre- and postpartum concentrations of insulin (P < 0.01), glucose (P < 0.001), triglyceride (P < 0.05), total cholesterol (P < 0.05), total protein (P < 0.01) and albumin (P < 0.01) compared with other treatments. The weight and BCS loss of these ewes were also lower during the pre- and postpartum periods (P < 0.05). In this study, except for day 124 of pregnancy, when the liver enzyme levels of the choline chloride supplemented ewes were numerically low, nutritional treatments did not affect the concentrations of these enzymes at other sampling points (P> 0.05). Regarding BHBA, NEFA and urea, FR and FR-MS ewes had a higher level of these parameters than other groups (P < 0.05). It was concluded that although Ghezel ewes showed some ability in tolerating late gestational feed restriction mainly through lipomobilization, supplementing the restricted diet with rumen-protected choline chloride combined with propylene glycol and monensin sodium during the final trimester of pregnancy could result in beneficial effects on metabolic status and finally good health and condition of animals.

The distinctive short-term response of late-pregnant prolific ewes to propylene glycol or glycerol drenching

Pregnancy toxemia is the most frequent metabolic disorder of ewes in late pregnancy. Although propylene glycol (PG) and glycerol (GLY) are common glucogenic supplements for treating pregnancy toxemia in ewes, the relative benefit of these 2 supplements is not entirely clear. Therefore, the objectives of the present study were to determine the changes during 24 h in key blood metabolites and insulin in response to PG or GLY drenching in prolific ewes. To this end, 36 multiparous late-pregnant Afec-Assaf ewes (∼132.4 d pregnant) bearing 2 to 4 fetuses, divided into 2 blocks (18 ewes in each block), with a blood β-hydroxybutyrate (BHB) concentration of 0.5 to 1.6 mmol/L were included. Ewes were divided into 3 groups (12 ewes each; 6 ewes in each experimental day), according to their BHB levels, expected litter size, body weight, and body condition score, and were drenched with the following: (1) control group (CTL), 55 mL of water; (2) PG, 106 mL of PG (100% PG, 448 calories); or (3) GLY, 108 mL of Koforin 80 (80% GL; 448 calories). Blood samples were taken before drenching and every hour after drenching for 24 h. Plasma concentration of glucose, BHB, nonesterified fatty acids, lactate, glycerol, and insulin were determined. Because there were no effects of treatments after 12 h in the first block, the data were analyzed for 12 h after drenching rather than 24 h. The plasma glucose concentration during the first 5 h after drenching was the highest in the GLY, BHB concentration was the lowest in the PG, and the nonesterified fatty acid levels were lower in the PG compared with the CTL ewes during the first 5 h after drenching. However, glucose concentration was higher in the PG ewes at 9, 11, and 12 h after drenching than in CTL or GLY ewes. The mean lactate concentration in plasma for 12 h was 2.5- and 1.9-fold higher in the PG compared with the CTL and GLY ewes, respectively, and except at 11 h after drenching, it was significantly higher at each time point. The insulin concentration was higher in the GLY than in both other groups at 2 to 5 h after drenching. These results suggest that during the first few hours after drenching the effect of PG was more effective in reducing the BHB concentration, whereas the GLY effect was more effective in enhancing glucose concentration. The increased concentration in lactate following PG treatment suggests that the PG contribution to gluconeogenesis is mediated through its metabolism to lactate. In contrast, the lack of an effect on lactate, and the faster increase in blood glucose in response to GLY suggest that GLY has a more advanced entry point to gluconeogenesis, which influences the immediate response in enhancing the glucose blood concentration.

Acute-phase proteins, cortisol and haematological parameters in ewes during the periparturient period.

Numerous subclinical diseases in sheep occur in the periparturient period and involve inflammatory processes; therefore, determining markers, such as acute-phase proteins (APPs), can allow an early diagnosis. Therefore, the objective of the present study was to assess changes in the plasma concentration of APPs and cortisol in clinically healthy ewes in the periparturient period for use in future studies. At the same time, haematological parameters were monitored. We showed that plasma APPs and cortisol concentrations were significantly higher in pregnant ewes than before insemination. A gradual increase in the SAA concentration was observed from the 14th day before to the day of parturition, while Hp was reduced from 2weeks before up to 2weeks after delivery. A significant increase in the Fb concentration was detected from the 14th day before to the 1st week after delivery. The cortisol concentration did not undergo significant changes in the periparturient period. We found an increase in the SAA and Fb concentrations and decrease in Hp in the periparturient period. The direction of the change in APPs of healthy ewes in the current study may be related to their distinct regulatory mechanisms during pregnancy. The APPs are usually altered during infection, inflammation, neoplasia, stress and trauma; therefore, knowing their reference values could help lead to an early diagnosis of subclinical forms of some diseases and pregnancy complications in ewes. The haematological analysis showed that ewes in late pregnancy and postpartum compared to dry period were under metabolic stress related to pregnancy and lactation.

Effect of Slow-Release Pellets of Selenium and Iodine on Performance and Some Blood Metabolites of Pregnant Moghani Ewes and Their Lambs.

The need for minerals during pregnancy in mammals increases by progress of gestation due to embryo development. Therefore, the supply of trace minerals for pregnant ewes in late pregnancy may be of vital importance. The present study was carried out to evaluate the effect of selenium and iodine release slowly on the performance and some blood parameters of pregnant ewes and their lambs. Experiments were performed on 40 pregnant ewes from 3 to 4years old in the last month of pregnancy with a weighing average of 52 ± 5kg and a factorial experiment (2 × 2) in a completely randomized design with 4 treatments and 10 replicates used for 120days (30days before and 90days after parturition of ewes). Experimental treatments were containing the following: (1) control, (2) ewe receiving bolus containing iodine, (3) ewe receiving bolus containing iodine and selenium, and (4) ewe receiving bolus containing selenium. One month before the expected birth date, the pellets will be fed to each of the livestock by bolus gun. During the test period, ewes will not receive any other mineral supplements and will be kept in a group in a herd. Ewes were blood sampled at 10days prepartum and 30 and 60days postpartum. The results showed that body weight on birth and weaning of lambs and average daily gain of lambs were highest in bloused animals that were related to the slow-release selenium tablet lambs (P < 0.05). Lambs born to mothers receiving 0.3mgday-1 of selenium per day had a keratin phosphokinase concentration of T4 concentration and T4 to T3 lower than that of zero selenium (P < 0.05). Daily gain, glutathione peroxidase activity, and T4 concentration at iodine 0.4mgday-1 were significantly higher than iodine level (P < 0.05). The adding of slow- release pellets of iodine and selenium improved the immune system.

Ewe nutrition and colostrum

To reduce the use of prophylactic antibiotics, nutrition of the ewe in late pregnancy needs to be addressed in order to maximise colostrum production and minimise neonatal mortality. A key aspect is the correct balance of energy and protein provided to the rumen micro-organisms. The volume and timing of colostrum administration is also essential as lambs cannot absorb colostrum after the first 36 hours of life and the immunoglobulin G concentration in colostrum decreases at a rate of 3.3 mg/kg/hour after parturition up to 23 hours at which the level becomes zero. Undernutrition decreases the quality and quantity of colostrum, and decreases the maternal bond with the lamb, which decreases the suckling of the ewe and, therefore, reduces the amount of colostrum the lamb ingests. Vet involvement through monitoring the metabolic profiles of the ewes can be beneficial in managing the nutrition of ewes in late pregnancy and the quality of colostrum produced.

Calcium and magnesium status of pregnant ewes grazing southern Australian pastures

During pregnancy, ewes graze pastures that may be marginal in calcium (Ca) and magnesium (Mg), and may also be low in sodium (Na) and high in potassium (K), with a high dietary cation–anion difference. Such pastures may increase susceptibility to hypocalcaemia and hypomagnesaemia, leading to lamb losses. Clinical hypocalcaemia and hypomagnesaemia do occur in Australian sheep; however, it is unknown whether subclinical forms of these disorders compromise ewe or lamb health and survival. The present study monitored the Ca and Mg status of ewes in late pregnancy, so as to evaluate the risk of subclinical mineral disorders in ewes grazing typical southern Australian pastures. Calcium and Mg concentrations in pasture, ewe plasma and urine were monitored in 15 flocks in southern Australia. Mineral concentrations in pasture did not indicate a widespread risk of Ca or Mg deficiency; however, urinary pH and Ca and Mg concentrations in the plasma and urine of the pregnant ewes were not entirely consistent with the expectations from pasture analysis. Urine pH was above 7 on all properties and 87.5% of properties had a mean Ca in urine below the adequate concentration of 1 µmol/mosmol. The mean plasma Ca concentration was below adequate (&lt;90 mg/L) on only one farm but five farms had more than 20% of ewes with below adequate Ca in plasma. In addition, although average farm concentrations of plasma and urine Mg indicated adequate Mg status of the ewes (&gt;18 mg/L), more than 20% of ewes on six farms had below adequate concentrations of plasma Mg. Only one-third of the farms had 100% of ewes measured with adequate concentrations of plasma Ca (4/15) or Mg (5/15). The mineral concentrations in pre-lambing blood and urine samples suggested that a significant number of animals grazing southern Australian pastures during winter may be at risk of subclinical hypocalcaemia and hypomagnesaemia.

The effect of organic selenium in feeding of ewes in late pregnancy on selenium transfer to progeny

ABSTRACT The objective of this study was to determine the effect of supplemented organic selenium at different levels to concentrate feed of Norduz ewes in late pregnancy on maternal serum, placenta, colostrum, and offspring serum concentrations. This study was conducted using two-year-old 35 Norduz ewes. Ewes were randomly assigned to one of three dietary treatments for 57 days prepartum and at the seven days postpartum. Group 1 was fed a standard pregnant sheep concentrate basal diet without any additional Se [...]

Can the amount of digestible undegraded protein offered to ewes during late pregnancy affect the performance and immune response of their offspring to gastrointestinal nematodes?

Maternal nutrition during pregnancy is a major environmental influence on foetal development with consequent effects on postnatal performance. We hypothesised that the level of intake of digestible undegraded protein (DUP) by the dam in late pregnancy would impact on the effectiveness of the immune response by offspring to gastrointestinal nematode infection. Eighty-five twin/triplet-bearing ewes, which were indoors from mid-pregnancy, were randomly assigned to 4 treatment groups for the final 6 weeks of pregnancy. Treatments were silage plus one of two iso-energetic and iso-nitrogenous concentrates (differing in DUP concentration; 29 and 94g/kg DM) offered at one of two feed levels (18/30 and 24/35kg in total for twin/triplet-bearing ewes, respectively). Ewes with triplets had one lamb removed at birth so that all ewes nursed 2 lambs when put to pasture as one flock in a 5-paddock rotational grazing system; all lambs were slaughtered after 29 weeks. Faecal egg count (FEC) and levels of serum IgA and IgE specific for Teladorsagia circumcincta were assessed for all lambs at various time points between 10 weeks of age and slaughter. Animal performance (live weight, live-weight gain, carcass weight) was recorded for all lambs. Worm burden at slaughter was determined for a sample of 12 lambs from each treatment. Nematodirus spp. FEC, ‘other strongyles’ FEC, and serum IgA and IgE specific for T. circumcincta were unaffected either by the concentration of DUP in the concentrate or by the level of concentrate offered to ewes in late pregnancy (P>0.1). Likewise, the dietary regime of the dams had no effect on lamb performance (P>0.1). It is concluded that increasing the DUP intake of ewes in late pregnancy had no effect on the immune response of their offspring to gastrointestinal nematode infection acquired through grazing naturally infected pasture.

Can the amount of digestible undegraded protein offered to ewes in late pregnancy affect the periparturient change in resistance to gastrointestinal nematodes?

Ewes experience a temporary decline in resistance to gastrointestinal nematodes (GIN) during the periparturient period, characterised by a rise in faecal egg count (FEC) that represents a major source of pasture contamination for naïve progeny. The aim of this study was to assess the effect of level of supplementation with digestible undegraded protein (DUP) during the last 6 weeks of pregnancy on periparturient FEC and the performance of ewes with a naturally acquired parasite infection. Eighty-five Belclare and Belclare x Scottish Blackface twin/triplet-bearing ewes were allocated to 1 of 4 dietary groups representing the combination of 2 concentrates (DUP concentration 29 and 94g/kg dry matter) with 2 levels of concentrate during the final 6 weeks of gestation (18 and 30kg in total for ewes with twins; 24 and 35kg for ewes with triplets). All ewes were housed during the pre-partum feeding period and offered grass silage ad libitum; food intake was recorded daily. The intake of DUP varied from 26 to 72g/d among treatments and was reflected in variation of 0.76 to 1.20 in metabolizable protein supply as a proportion of requirements. After lambing, ewes and lambs grazed on permanent sheep pasture, without concentrate supplementation, until weaning (14 weeks post lambing). The variables studied, from week 6 pre-lambing up to week 10 post-lambing, included: FEC, serum pepsinogen concentration, body weight (BW) and body condition score (BCS). The effect of week (relative to lambing date) on FEC was highly significant (P<0.001). However, diet did not influence FEC (P>0.05) at any stage either pre- or post-partum. Pepsinogen concentration also varied with time but was not influenced by dietary treatment (P>0.05). The changes in BW and BCS from 6 weeks before lambing to weaning were not affected by the concentration of DUP in the supplement but ewes on treatments involving the higher level of supplementation lost less BW and BCS (P<0.001). The results of this study indicate that the level of DUP supplementation during the last 6 weeks of pregnancy does not affect FEC, BW or BCS of housed ewes with a naturally acquired GIN infection.

Blood biochemical indicators and concentration of thyroid hormones in heavily pregnant and lactating ewes depending on selenium supplementation

The aim of this research was to determine the effect of dietary selenium supplementation (organic and inorganic) on the biochemical indices and thyroid hormone concentration in the blood of heavily pregnant and lactating ewes. Research was conducted on 30 pregnant ewes of the Merinolandschaf breed divided into 3 groups, and lasted for four months (two months on ewes in late pregnancy and two months on ewes during lactation). The first group was given a basal diet without the addition of selenium. The feed mixture of group 2 was supplemented with 0.3 mg/kg of organic sources of selenium, and group 3 with the same amount of inorganic sources of selenium. In lactating ewes, organic and inorganic selenium lead to a significant (P&lt; 0.05;P&lt; 0.01) increase in urea concentration. At the same time, in lactating ewes inorganic selenium had a significant decreasing effect (P&lt; 0.05;P&lt; 0.01) on cholesterol, low-density lipoprotein and triglycerides. Organic selenium in heavily pregnant ewes significantly (P&lt; 0.01) increased aspartate aminotransferase activity in comparison to group 1 and 3. In lactating ewes significant (P&lt; 0.05) differences in aspartate aminotransferase activity were determined between groups 2 and 3. With selenium supplementation a significant (P&lt; 0.01;P&lt; 0.05) increase concentration of Se was determined (for heavily pregnant and lactating ewes, respectively), as well as Na, Cl, Fe (lactating ewes), and a decrease of K (high pregnant ewes). Organic selenium had an effect on the increase of triiodothyronine in lactating ewes. We can conclude that selenium supplementation contributes to good health and condition of ewes in the demanding period of lactation.

PHYSIOLOGICAL RESPONSES AND LACTATIONAL PERFORMANCE OF FARAFRA AND SAIDI SHEEP AS AFFECTED BY SUPPLEMENTATION OF PROBIOTIC WITH NATURAL ANTIOXIDANTS

A total of 88 ewes were used in this study in two trails (44 per each trail) to investigate the effects of supplementation of probiotic abbreviated as (Effective Microorganisms with Medicinal Herbs-EMMH) on physiological responses during thermonuteral and heat stress conditions and milk yield of two local breeds (Farafra and Saidi). The studied probiotic consisted of mixed culture of two strains of fungi (Trichoderma reesei and Aspergillus oryzae) with dry live baker's yeast (Saccharomyces cervisiae) and the mixed culture was enriched with natural antioxidants of herbal medicine plants. In the first trail, 22 Farafra and 22 Saidi ewes were used during summer, along mating and pregnancy seasons until parturition. In the second trail, 22 Farafra and 22 Saidi ewes in late pregnancy were used. Ewes of every breed were divided homogonously into two equal groups (11 ewes per each). Group (I) fed basal diet (concentrate feed mixture + rice straw). Group (II) fed basal diet + 3 kg EMMH/ton. Reproductive traits were estrus duration, conception rate (%) and gestational period, number of lambs born, number of twins and birth weight was recorded. Thermal responses, gas exchange parameters and heat production measurement were determined during thermoneutral (comfort) conditions and during heat stress condition (after exposing animals to direct sun for 2 hours). Also, climatological data were recorded periodically. Daily and total milk yield were measured along the lactation season using lambs sucking technique. Body weight gain of suckling lambs, weaning weight and survival rate were recorded. Also, values of heamatocrit % (Ht), total protein (TP) sodium (Na), Potassium (K) and total antioxidant capacity (TAC) were determined in ewes. In thermonuteral conditions, EMMH supplemented group had significantly (P< 0.05) lower rectal temperature than control group in Farafra ewes. Whereas, in heat stress conditions, rectal and skin temperature significantly (P< 0.05) decreased in EMMH supplemented group in both breeds. Respiration rate was significantly (P< 0.05) lower in supplemented group than control in Farafra ewes only. Tidal volume was significantly (P< 0.05) higher in EMMH group. While, metabolic rate and heat production values were significantly (P< 0.05) decreased in EMMH supplemented group. Average body weight and body weight change of ewes significantly (P <0.05) improved by EMMH supplementation. Conception rate was improved in EMMH group compared to control in both breeds (90.9% vs. 72.7% in Farafra ewes) and (81.8 vs. 72.7% in Saidi ewes). Furthermore, average litter size per ewe, number of lambs born per group, number of twins born per group were improved by EMMH supplementation. Total milk yield, average daily milk yield per ewe, weaning weight and total gain of lambs were significantly (P<0.05) improved by EMMH supplementation in both breeds. Also, survival rate was improved in EMMH group in both breeds. Values of total protein concentration (TP) and total antioxidant capacity (TAC) was significantly (P <0.05) higher in EMMH supplemented group in both Farafra and Saidi. From the present results, it can be concluded that supplementation of EMMH probiotic had a favorable effect in improving physiological responses of animals during heat stress and can ameliorate the adverse effects of heat stress. Also, it led to improve lactational performance of ewes and the performance of their suckling lambs.

Production responses of reproducing ewes to a by-product-based diet inoculated with the probiotic Bacillus amyloliquefaciens strain H57

The potential application of the spore-forming probiotic Bacillus amyloliquefaciens strain H57 (H57) as a novel probiotic for ruminants was evaluated in reproducing ewes. Performance responses were determined by delivering H57 in a pelleted diet based mainly on palm kernel meal (PKM) and sorghum grain. PKM is an agro-industrial by-product with a reputation for poor palatability and the availability of the starch in sorghum grain can be limited in ruminants. The hypothesis was that H57 improves the feeding value of a relatively low quality concentrate diet. Twenty-four first-parity white Dorper ewes were fed PKM-based pellets manufactured with or without H57 (109 cfu/kg pellet) in late pregnancy. During this phase of late pregnancy, the H57 ewes ate 17% more dry matter (1019 vs 874 g/day, P = 0.03), gained more weight (194 vs 30 g/day, P = 0.008) and retained more nitrogen (6.13 vs 3.34 g/day, P = 0.01), but produced lambs with a similar birthweight (4.1 vs 4.2 kg, P = 0.73). Rumen fluid collected from H57 ewes in late pregnancy had higher pH (7.1 vs 6.8, P = 0.07), acetate : propionate ratio (3.4 vs 2.7, P = 0.04), lower ammonia (69 vs 147 mmol/L, P = 0.001) and total volatile fatty acid concentrations (40 vs 61 mg/L, P = 0.02). The digestibility of dry matter, organic matter and fibre were similar between the two groups. The lambs of the H57 ewes grew faster than those of the Control ewes for the first 21 days of lactation (349 vs 272 g/day, P = 0.03), but not thereafter. H57 can improve feed intake and maternal liveweight gain in late pregnancy of first-parity ewes fed a diet based on PKM.

Growth and Haemato-Biochemical Response in Lambs in Relation to Supplementary Feeding of Ewes in Late Pregnancy

A study was undertaken to elucidate the effect of concentrate supplementation (eight weeks before the expected date of lambing) to native ewes in coastal Odisha on lamb growth and haemato-biochemical profile. Forty pregnant ewes (12 months old) were randomly distributed into two groups (T-1 and T-2). Ewes of T-1 were maintained on grazing only while ewes of T-2 were supplemented with concentrate mixture @ 200 g/sheep/day along with grazing. Significantly (P<0.01) higher birth weight and growth was observed in T-2 group lambs. The lambs in T-2 showed significantly (P<0.05) higher haemoglobin, packed cell volume, blood glucose, total protein and globulin values in the pre-weaning period than those in T-1. It was concluded that pre-partum concentrate feeding @ 200 g/day/ewe to the pregnant ewe improved birth weight and growth rate of lambs.

L-Arginine Modulates Maternal Hormonal Profiles and Neonatal Traits during Two Stages of Pregnancy in Sheep

A 2 × 2 factorial arrangement was designed to test effects of supplementation of a low (L, 75 mg/kg BW) vs. high (H, 150 mg/kg BW) L-arginine given at early (first 56 days) vs. late (last 56 days) pregnancy on maternal hormones and neonatal traits. Thirty Najdi pregnant ewes were randomly allocated into 6 groups. Ewes in G1 and G2 served as controls (C), given 50 ml saline at either early (CE) or late (CL) pregnancy, respectively. G3 and G4 ewes in early pregnancy received low (LE) and high L-arginine (HE), respectively. G5 and G6 ewes in late pregnancy received low (LL) and high (HL) L-arginine, respectively. A weekly blood sample was collected from initiation of the treatment till parturition. Serum growth hormone (GH), insulin-like growth factor-I (IGF-I), insulin, progesterone (P4) and estradiol 17 β (E2) profiles were determined. Neonatal traits were also determined. Insulin was higher (P 0.05). Lamb survival rates at birth in LE ewes were highest (100%) compared to other treatments. In conclusion, supplementing pregnant ewes with low dosage of L-arginine at early stage of gestation increased lamb birth weight and survival, and improved maternal health.

Grazing crops: implications for reproducing sheep

Integration of crops and livestock has been revitalised in Australia, initially as an opportunity to increase cropping within the high-rainfall grazing zones, and more recently, to improve enterprise diversification and profitability across the low-, medium- and high-rainfall, and mixed-farming zones. Young crops are highly digestible (&gt;80% dry matter digestibility, DMD) with a high energy density (&gt;12 MJ/kg DM) and, in much of southern Australia, fill a winter feed gap. The quality and time of feed availability also coincide with the high nutrient requirements of ewes in late pregnancy and lactation. In Western Australia and South Australia, young crops are available for lactating ewes and young growing lambs (autumn lambing). For the smaller proportion of growers who lamb later in winter, young crops are available for the last 1–2 months of pregnancy. In the later-lambing states of New South Wales and Victoria, crops may be grazed by ewes at any stage of pregnancy and lactation and/or by young lambs. In Tasmania, crops are more likely to be available during early–mid-gestation. Limited studies on feed budgeting with grazing crops have indicated that ewes can maintain or even increase liveweight, with a much lower level of feed on offer than would be required with traditional pastures (&lt;500 kg DM/ha). This has the potential to increase whole-farm stocking rates and/or reduce fetal mortality, increase lamb birthweight and survival and improve lifetime production. Maintaining or increasing ewe liveweight during pregnancy and lactation may also result in heavier ewes the following year and higher ovulation rates. Pregnancy and lactation are also periods of increased susceptibility to metabolic disturbances. The composition of young crops increases this susceptibility. Pregnancy toxaemia, hypocalcaemia and hypomagnesaemia can influence ewe health and fetal survival. Chronic acidosis and excessive ammonia absorption from rapid introduction of pregnant ewes onto young crops may risk appetite loss and increase susceptibility to pregnancy toxaemia. Low magnesium and sodium combined with high potassium increases the risk of grass tetany. Most young crops (except canola) also have a tetany index &gt;2.2, indicating a high risk of grass tetany. The elevated potassium also contributes to a high dietary cation–anion difference of approximately +49 mEq/100 g DM and this may cause metabolic alkalosis and hypocalcaemia. Pregnancy toxaemia, hypocalcaemia and grass tetany are all potential causes of increased ewe mortality. Pregnancy and/or lactation outcomes will also be influenced by a deficiency of trace elements. Grazing young crops in areas with a history of selenium, copper, iodine and cobalt deficiency will increase susceptibility to deficiency by increasing growth and feed intake. In conclusion, the grazing of young growing crops presents new opportunities for increased production and stocking rates in the mixed-farming zones. The value of this feed source is well recognised by some producers. While growing crops have a highly productive potential, they also come with an increased risk of a range metabolic disturbances and nutritional imbalances. These risks can be minimised by regular monitoring of livestock and crop biomass and the provision of mineral supplements.

Effect of vitamin E supplementation to ewes in late pregnancy on the rate of stillborn lambs

This study evaluated the effect of supplemental vitamin E to ewes in late pregnancy on the rate of stillborn lambs. Ewes in 19 flocks in 5 regions of Norway were daily supplemented the 6–7 weeks before average expected lambing date with either (1) 360IU of vitamin E (supplemented), or (2) placebo (control). The daily supplement was given in addition to the daily basal diet of forage, concentrate and mixture of minerals and vitamins in each flock, assuming that forage contained on average 40mg α-tocopherol/kg DM. Information about the basal diets was collected via analyses of forage samples and questionnaires. Blood was collected from a sample of ewes in each flock 1 week pre-treatment (7–8 weeks before lambing), and from some flocks 1–2 weeks after initiation of supplementation (5–6 weeks before lambing) and 1–2 weeks after lambing. The body condition score (BCS) of the ewes was assessed 4–6 weeks before lambing. Mean content (±SD) of α-tocopherol in the forage of the 19 flocks was 25±17mg/kg DM. Mean daily intake of vitamin E of the ewes from the basal diet was 153±44IU. Vitamin E supplementation increased serum concentration of α-tocopherol (P=0.0002) 5–6 weeks before lambing. After lambing there was a higher ratio of α-tocopherol to cholesterol in serum of supplemented ewes compared with control ewes (P=0.02). Ewes with one or more stillborn lambs had lower serum α-tocopherol concentrations than ewes without stillborn lambs in litters with ≥3 lambs (P=0.01). For ewes with ≥3 lambs there was a significant lower stillbirth rate for vitamin E supplemented than for control ewes (P=0.0004), while there was no effect on the stillbirth rate for ewes having ≤2 lambs. Ewes with low BCS had a higher stillbirth rate than ewes with medium or high BCS (P=0.001). The results of this study indicate that daily supplementation of vitamin E during the last 6–7 weeks before lambing decreases the stillbirth rate of ewes having ≥3 lambs.